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Protein

UDP-glycosyltransferase 76C2

Gene

UGT76C2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the N-glucosylation of cytokinins. Catalyzes the formation of both the 7-N and the 9-N-glucosides.1 Publication

Enzyme regulationi

Inhibited by olomoucine and 3-isobutyl-1-methylxanthine.

Kineticsi

  1. KM=0.22 mM for trans-zeatin (at 30 degrees Celsius and pH 7.0)1 Publication
  2. KM=0.22 mM for dihydrozeatin (at 30 degrees Celsius and pH 7.0)1 Publication
  3. KM=0.07 mM for N6-isopentenyladenine (at 30 degrees Celsius and pH 7.0)1 Publication
  4. KM=0.04 mM for N6-benzyladenine (at 30 degrees Celsius and pH 7.0)1 Publication
  5. KM=0.07 mM for kinetin (at 30 degrees Celsius and pH 7.0)1 Publication
  1. Vmax=5.91 nmol/sec/mg enzyme with trans-zeatin as substrate1 Publication
  2. Vmax=10.78 nmol/sec/mg enzyme with dihydrozeatin as substrate1 Publication
  3. Vmax=10.42 nmol/sec/mg enzyme with N6-isopentenyladenine as substrate1 Publication
  4. Vmax=7.90 nmol/sec/mg enzyme with N6-benzyladenine as substrate1 Publication
  5. Vmax=3.63 nmol/sec/mg enzyme with kinetin as substrate1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei272UDP-glucoseBy similarity1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway involved in stomatal movement Source: TAIR
  • cellular response to water deprivation Source: TAIR
  • cytokinin metabolic process Source: TAIR
  • regulation of anthocyanin catabolic process Source: TAIR
  • seed development Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT5G05860-MONOMER
BRENDAi2.4.1.118 399
SABIO-RKQ9FIA0

Protein family/group databases

CAZyiGT1 Glycosyltransferase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 76C2 (EC:2.4.1.-)
Alternative name(s):
Cytokinin-N-glucosyltransferase 2
Gene namesi
Name:UGT76C2
Ordered Locus Names:At5g05860
ORF Names:MJJ3.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G05860
TAIRilocus:2166444 AT5G05860

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741541 – 450UDP-glycosyltransferase 76C2Add BLAST450

Proteomic databases

PaxDbiQ9FIA0
PRIDEiQ9FIA0

Expressioni

Gene expression databases

ExpressionAtlasiQ9FIA0 baseline and differential
GenevisibleiQ9FIA0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05860.1

Structurei

3D structure databases

ProteinModelPortaliQ9FIA0
SMRiQ9FIA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni331 – 333UDP-glucose bindingBy similarity3
Regioni348 – 356UDP-glucose bindingBy similarity9
Regioni370 – 373UDP-glucose bindingBy similarity4

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG1192 Eukaryota
COG1819 LUCA
HOGENOMiHOG000237564
InParanoidiQ9FIA0
KOiK13493
OMAiIRWAPQQ
OrthoDBiEOG09360BKY
PhylomeDBiQ9FIA0

Family and domain databases

InterProiView protein in InterPro
IPR002213 UDP_glucos_trans
PfamiView protein in Pfam
PF00201 UDPGT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FIA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKRNGLRV ILFPLPLQGC INPMLQLANI LHVRGFSITV IHTRFNAPKA
60 70 80 90 100
SSHPLFTFLQ IPDGLSETEI QDGVMSLLAQ INLNAESPFR DCLRKVLLES
110 120 130 140 150
KESERVTCLI DDCGWLFTQS VSESLKLPRL VLCTFKATFF NAYPSLPLIR
160 170 180 190 200
TKGYLPVSES EAEDSVPEFP PLQKRDLSKV FGEFGEKLDP FLHAVVETTI
210 220 230 240 250
RSSGLIYMSC EELEKDSLTL SNEIFKVPVF AIGPFHSYFS ASSSSLFTQD
260 270 280 290 300
ETCILWLDDQ EDKSVIYVSL GSVVNITETE FLEIACGLSN SKQPFLWVVR
310 320 330 340 350
PGSVLGAKWI EPLSEGLVSS LEEKGKIVKW APQQEVLAHR ATGGFLTHNG
360 370 380 390 400
WNSTLESICE GVPMICLPGG WDQMLNSRFV SDIWKIGIHL EGRIEKKEIE
410 420 430 440 450
KAVRVLMEES EGNKIRERMK VLKDEVEKSV KQGGSSFQSI ETLANHILLL
Length:450
Mass (Da):50,425
Last modified:March 1, 2001 - v1
Checksum:iB04568214C4CC857
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017060, AB005237 Genomic DNA Translation: BAB10791.1
CP002688 Genomic DNA Translation: AED90933.1
AY045617 mRNA Translation: AAK73975.1
AY143896 mRNA Translation: AAN28835.1
RefSeqiNP_196205.1, NM_120668.4
UniGeneiAt.25866
At.32941

Genome annotation databases

EnsemblPlantsiAT5G05860.1; AT5G05860.1; AT5G05860
GeneIDi830471
GrameneiAT5G05860.1; AT5G05860.1; AT5G05860
KEGGiath:AT5G05860

Similar proteinsi

Entry informationi

Entry nameiU76C2_ARATH
AccessioniPrimary (citable) accession number: Q9FIA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health