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Protein

UDP-glycosyltransferase 76C2

Gene

UGT76C2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the N-glucosylation of cytokinins. Catalyzes the formation of both the 7-N and the 9-N-glucosides.1 Publication

Enzyme regulationi

Inhibited by olomoucine and 3-isobutyl-1-methylxanthine.

Kineticsi

  1. KM=0.22 mM for trans-zeatin (at 30 degrees Celsius and pH 7.0)1 Publication
  2. KM=0.22 mM for dihydrozeatin (at 30 degrees Celsius and pH 7.0)1 Publication
  3. KM=0.07 mM for N6-isopentenyladenine (at 30 degrees Celsius and pH 7.0)1 Publication
  4. KM=0.04 mM for N6-benzyladenine (at 30 degrees Celsius and pH 7.0)1 Publication
  5. KM=0.07 mM for kinetin (at 30 degrees Celsius and pH 7.0)1 Publication
  1. Vmax=5.91 nmol/sec/mg enzyme with trans-zeatin as substrate1 Publication
  2. Vmax=10.78 nmol/sec/mg enzyme with dihydrozeatin as substrate1 Publication
  3. Vmax=10.42 nmol/sec/mg enzyme with N6-isopentenyladenine as substrate1 Publication
  4. Vmax=7.90 nmol/sec/mg enzyme with N6-benzyladenine as substrate1 Publication
  5. Vmax=3.63 nmol/sec/mg enzyme with kinetin as substrate1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei272 – 2721UDP-glucoseBy similarity

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway involved in stomatal movement Source: TAIR
  • cellular response to water deprivation Source: TAIR
  • cytokinin metabolic process Source: TAIR
  • flavonoid biosynthetic process Source: GO_Central
  • flavonoid glucuronidation Source: GO_Central
  • regulation of anthocyanin catabolic process Source: TAIR
  • seed development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT5G05860-MONOMER.
BRENDAi2.4.1.118. 399.
SABIO-RKQ9FIA0.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 76C2 (EC:2.4.1.-)
Alternative name(s):
Cytokinin-N-glucosyltransferase 2
Gene namesi
Name:UGT76C2
Ordered Locus Names:At5g05860
ORF Names:MJJ3.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G05860.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450UDP-glycosyltransferase 76C2PRO_0000074154Add
BLAST

Proteomic databases

PaxDbiQ9FIA0.
PRIDEiQ9FIA0.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FIA0. baseline and differential.
GenevisibleiQ9FIA0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05860.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FIA0.
SMRiQ9FIA0. Positions 10-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni331 – 3333UDP-glucose bindingBy similarity
Regioni348 – 3569UDP-glucose bindingBy similarity
Regioni370 – 3734UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
HOGENOMiHOG000237564.
InParanoidiQ9FIA0.
KOiK13493.
OMAiPMICLPG.
OrthoDBiEOG09360BKY.
PhylomeDBiQ9FIA0.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FIA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKRNGLRV ILFPLPLQGC INPMLQLANI LHVRGFSITV IHTRFNAPKA
60 70 80 90 100
SSHPLFTFLQ IPDGLSETEI QDGVMSLLAQ INLNAESPFR DCLRKVLLES
110 120 130 140 150
KESERVTCLI DDCGWLFTQS VSESLKLPRL VLCTFKATFF NAYPSLPLIR
160 170 180 190 200
TKGYLPVSES EAEDSVPEFP PLQKRDLSKV FGEFGEKLDP FLHAVVETTI
210 220 230 240 250
RSSGLIYMSC EELEKDSLTL SNEIFKVPVF AIGPFHSYFS ASSSSLFTQD
260 270 280 290 300
ETCILWLDDQ EDKSVIYVSL GSVVNITETE FLEIACGLSN SKQPFLWVVR
310 320 330 340 350
PGSVLGAKWI EPLSEGLVSS LEEKGKIVKW APQQEVLAHR ATGGFLTHNG
360 370 380 390 400
WNSTLESICE GVPMICLPGG WDQMLNSRFV SDIWKIGIHL EGRIEKKEIE
410 420 430 440 450
KAVRVLMEES EGNKIRERMK VLKDEVEKSV KQGGSSFQSI ETLANHILLL
Length:450
Mass (Da):50,425
Last modified:March 1, 2001 - v1
Checksum:iB04568214C4CC857
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017060, AB005237 Genomic DNA. Translation: BAB10791.1.
CP002688 Genomic DNA. Translation: AED90933.1.
AY045617 mRNA. Translation: AAK73975.1.
AY143896 mRNA. Translation: AAN28835.1.
RefSeqiNP_196205.1. NM_120668.3.
UniGeneiAt.25866.
At.32941.

Genome annotation databases

EnsemblPlantsiAT5G05860.1; AT5G05860.1; AT5G05860.
GeneIDi830471.
GrameneiAT5G05860.1; AT5G05860.1; AT5G05860.
KEGGiath:AT5G05860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017060, AB005237 Genomic DNA. Translation: BAB10791.1.
CP002688 Genomic DNA. Translation: AED90933.1.
AY045617 mRNA. Translation: AAK73975.1.
AY143896 mRNA. Translation: AAN28835.1.
RefSeqiNP_196205.1. NM_120668.3.
UniGeneiAt.25866.
At.32941.

3D structure databases

ProteinModelPortaliQ9FIA0.
SMRiQ9FIA0. Positions 10-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G05860.1.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbiQ9FIA0.
PRIDEiQ9FIA0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G05860.1; AT5G05860.1; AT5G05860.
GeneIDi830471.
GrameneiAT5G05860.1; AT5G05860.1; AT5G05860.
KEGGiath:AT5G05860.

Organism-specific databases

TAIRiAT5G05860.

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
HOGENOMiHOG000237564.
InParanoidiQ9FIA0.
KOiK13493.
OMAiPMICLPG.
OrthoDBiEOG09360BKY.
PhylomeDBiQ9FIA0.

Enzyme and pathway databases

BioCyciMetaCyc:AT5G05860-MONOMER.
BRENDAi2.4.1.118. 399.
SABIO-RKQ9FIA0.

Miscellaneous databases

PROiQ9FIA0.

Gene expression databases

ExpressionAtlasiQ9FIA0. baseline and differential.
GenevisibleiQ9FIA0. AT.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiU76C2_ARATH
AccessioniPrimary (citable) accession number: Q9FIA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.