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Protein

UDP-glycosyltransferase 76C1

Gene

UGT76C1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the N-glucosylation of cytokinins. Catalyzes the formation of both the 7-N and the 9-N-glucosides.1 Publication

Enzyme regulationi

Inhibited by olomoucine and 3-isobutyl-1-methylxanthine.

Kineticsi

  1. KM=0.24 mM for trans-zeatin (at 30 degrees Celsius and pH 7.0)1 Publication
  2. KM=0.16 mM for dihydrozeatin (at 30 degrees Celsius and pH 7.0)1 Publication
  3. KM=0.13 mM for N6-isopentenyladenine (at 30 degrees Celsius and pH 7.0)1 Publication
  4. KM=0.09 mM for N6-benzyladenine (at 30 degrees Celsius and pH 7.0)1 Publication
  5. KM=0.21 mM for kinetin (at 30 degrees Celsius and pH 7.0)1 Publication
  1. Vmax=4.06 nmol/sec/mg enzyme with trans-zeatin as substrate1 Publication
  2. Vmax=6.34 nmol/sec/mg enzyme with dihydrozeatin as substrate1 Publication
  3. Vmax=18.67 nmol/sec/mg enzyme with N6-isopentenyladenine as substrate1 Publication
  4. Vmax=15.93 nmol/sec/mg enzyme with N6-benzyladenine as substrate1 Publication
  5. Vmax=9.40 nmol/sec/mg enzyme with kinetin as substrate1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei279UDP-glucoseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT5G05870-MONOMER
BRENDAi2.4.1.118 399
SABIO-RKiQ9FI99

Protein family/group databases

CAZyiGT1 Glycosyltransferase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 76C1 (EC:2.4.1.-)
Alternative name(s):
Cytokinin-N-glucosyltransferase 1
Gene namesi
Name:UGT76C1
Ordered Locus Names:At5g05870
ORF Names:K18J17.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G05870
TAIRilocus:2153614 AT5G05870

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741531 – 464UDP-glycosyltransferase 76C1Add BLAST464

Proteomic databases

PaxDbiQ9FI99

Expressioni

Gene expression databases

ExpressionAtlasiQ9FI99 baseline and differential
GenevisibleiQ9FI99 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05870.1

Structurei

3D structure databases

ProteinModelPortaliQ9FI99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni338 – 340UDP-glucose bindingBy similarity3
Regioni355 – 363UDP-glucose bindingBy similarity9
Regioni377 – 380UDP-glucose bindingBy similarity4

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG1192 Eukaryota
COG1819 LUCA
HOGENOMiHOG000237564
InParanoidiQ9FI99
KOiK13493
OMAiIHDVPAS
OrthoDBiEOG09360BKY
PhylomeDBiQ9FI99

Family and domain databases

InterProiView protein in InterPro
IPR002213 UDP_glucos_trans
PfamiView protein in Pfam
PF00201 UDPGT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FI99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKRNERQVI LFPLPLQGCI NPMLQLAKIL YSRGFSITII HTRFNAPKSS
60 70 80 90 100
DHPLFTFLQI RDGLSESQTQ SRDLLLQLTL LNNNCQIPFR ECLAKLIKPS
110 120 130 140 150
SDSGTEDRKI SCVIDDSGWV FTQSVAESFN LPRFVLCAYK FSFFLGHFLV
160 170 180 190 200
PQIRREGFLP VPDSEADDLV PEFPPLRKKD LSRIMGTSAQ SKPLDAYLLK
210 220 230 240 250
ILDATKPASG IIVMSCKELD HDSLAESNKV FSIPIFPIGP FHIHDVPASS
260 270 280 290 300
SSLLEPDQSC IPWLDMRETR SVVYVSLGSI ASLNESDFLE IACGLRNTNQ
310 320 330 340 350
SFLWVVRPGS VHGRDWIESL PSGFMESLDG KGKIVRWAPQ LDVLAHRATG
360 370 380 390 400
GFLTHNGWNS TLESICEGVP MICLPCKWDQ FVNARFISEV WRVGIHLEGR
410 420 430 440 450
IERREIERAV IRLMVESKGE EIRGRIKVLR DEVRRSVKQG GSSYRSLDEL
460
VDRISIIIEP LVPT
Length:464
Mass (Da):52,360
Last modified:March 1, 2001 - v1
Checksum:i522E2121F643896D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017060 Genomic DNA Translation: BAB10792.1
CP002688 Genomic DNA Translation: AED90934.1
BT006473 mRNA Translation: AAP21281.1
AK228311 mRNA Translation: BAF00254.1
RefSeqiNP_196206.1, NM_120669.4
UniGeneiAt.32941

Genome annotation databases

EnsemblPlantsiAT5G05870.1; AT5G05870.1; AT5G05870
GeneIDi830472
GrameneiAT5G05870.1; AT5G05870.1; AT5G05870
KEGGiath:AT5G05870

Similar proteinsi

Entry informationi

Entry nameiU76C1_ARATH
AccessioniPrimary (citable) accession number: Q9FI99
Secondary accession number(s): Q0WRJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health