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Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136NADBy similarity1
Binding sitei237NADBy similarity1
Binding sitei242SpermidineBy similarity1
Binding sitei284NAD; via amide nitrogenBy similarity1
Binding sitei289SpermidineBy similarity1
Active sitei330NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 108NADBy similarity5
Nucleotide bindingi130 – 132NADBy similarity3
Nucleotide bindingi309 – 310NADBy similarity2
Nucleotide bindingi343 – 344NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • embryo sac development Source: TAIR
  • peptidyl-lysine modification to peptidyl-hypusine Source: GO_Central

Keywordsi

Molecular functionTransferase
Biological processHypusine biosynthesis
LigandNAD

Enzyme and pathway databases

BioCyciARA:AT5G05920-MONOMER
ReactomeiR-ATH-204626 Hypusine synthesis from eIF5A-lysine
UniPathwayiUPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
Ordered Locus Names:At5g05920
ORF Names:K18J17_7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G05920
TAIRilocus:2153664 AT5G05920

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344731 – 368Deoxyhypusine synthaseAdd BLAST368

Proteomic databases

PaxDbiQ9FI94
PRIDEiQ9FI94

PTM databases

iPTMnetiQ9FI94

Expressioni

Gene expression databases

ExpressionAtlasiQ9FI94 baseline and differential
GenevisibleiQ9FI94 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05920.1

Structurei

3D structure databases

ProteinModelPortaliQ9FI94
SMRiQ9FI94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 136Spermidine bindingBy similarity2
Regioni315 – 317Spermidine bindingBy similarity3
Regioni324 – 330Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924 Eukaryota
COG1899 LUCA
HOGENOMiHOG000228838
InParanoidiQ9FI94
KOiK00809
OMAiGWEDSTM
OrthoDBiEOG09360CE8
PhylomeDBiQ9FI94

Family and domain databases

Gene3Di3.40.910.101 hit
InterProiView protein in InterPro
IPR002773 Deoxyhypusine_synthase
IPR036982 Deoxyhypusine_synthase_sf
IPR029035 DHS-like_NAD/FAD-binding_dom
PANTHERiPTHR11703 PTHR11703, 1 hit
PfamiView protein in Pfam
PF01916 DS, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00321 dhys, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FI94-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDDRVFSSV HSTVFKESES LEGKCDKIEG YDFNQGVDYP KLMRSMLTTG
60 70 80 90 100
FQASNLGEAI DVVNQMLDWR LADETTVAED CSEEEKNPSF RESVKCKIFL
110 120 130 140 150
GFTSNLVSSG VRDTIRYLVQ HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF
160 170 180 190 200
SLPGAYLRSK GLNRIGNLLV PNDNYCKFED WIIPIFDEML KEQKEENVLW
210 220 230 240 250
TPSKLLARLG KEINNESSYL YWAYKMNIPV FCPGLTDGSL GDMLYFHSFR
260 270 280 290 300
TSGLIIDVVQ DIRAMNGEAV HANPKKTGMI ILGGGLPKHH ICNANMMRNG
310 320 330 340 350
ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL
360
LVAETFATKR DQTCESKT
Length:368
Mass (Da):41,064
Last modified:March 1, 2001 - v1
Checksum:i491D9DF362DAB002
GO

Sequence cautioni

The sequence AAK32840 differs from that shown. Reason: Frameshift at position 68.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29E → Q in AAG53642 (PubMed:11278418).Curated1
Sequence conflicti67Missing in AAK32840 (PubMed:14593172).Curated1
Sequence conflicti309T → P in AAG53642 (PubMed:11278418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296078 mRNA Translation: AAG53642.2
AB017060 Genomic DNA Translation: BAB10797.1
CP002688 Genomic DNA Translation: AED90939.1
AF361828 mRNA Translation: AAK32840.1 Frameshift.
AY056066 mRNA Translation: AAL06966.1
AY087829 mRNA Translation: AAM65382.1
RefSeqiNP_196211.1, NM_120674.4 [Q9FI94-1]
UniGeneiAt.9443

Genome annotation databases

EnsemblPlantsiAT5G05920.1; AT5G05920.1; AT5G05920 [Q9FI94-1]
GeneIDi830477
GrameneiAT5G05920.1; AT5G05920.1; AT5G05920 [Q9FI94-1]
KEGGiath:AT5G05920

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDHYS_ARATH
AccessioniPrimary (citable) accession number: Q9FI94
Secondary accession number(s): Q9ASU5, Q9C5Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome