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Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361NADBy similarity
Binding sitei237 – 2371NADBy similarity
Binding sitei242 – 2421SpermidineBy similarity
Binding sitei284 – 2841NAD; via amide nitrogenBy similarity
Binding sitei289 – 2891SpermidineBy similarity
Active sitei330 – 3301NucleophileBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1085NADBy similarity
Nucleotide bindingi130 – 1323NADBy similarity
Nucleotide bindingi309 – 3102NADBy similarity
Nucleotide bindingi343 – 3442NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT5G05920-MONOMER.
ARA:GQT-45-MONOMER.
ReactomeiR-ATH-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
Ordered Locus Names:At5g05920
ORF Names:K18J17_7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G05920.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Deoxyhypusine synthasePRO_0000134473Add
BLAST

Proteomic databases

PaxDbiQ9FI94.
PRIDEiQ9FI94.

PTM databases

iPTMnetiQ9FI94.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FI94. baseline.
GenevisibleiQ9FI94. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05920.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FI94.
SMRiQ9FI94. Positions 27-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1362Spermidine bindingBy similarity
Regioni315 – 3173Spermidine bindingBy similarity
Regioni324 – 3307Spermidine bindingBy similarity

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
HOGENOMiHOG000228838.
InParanoidiQ9FI94.
OMAiSWGKIKE.
PhylomeDBiQ9FI94.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FI94-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDDRVFSSV HSTVFKESES LEGKCDKIEG YDFNQGVDYP KLMRSMLTTG
60 70 80 90 100
FQASNLGEAI DVVNQMLDWR LADETTVAED CSEEEKNPSF RESVKCKIFL
110 120 130 140 150
GFTSNLVSSG VRDTIRYLVQ HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF
160 170 180 190 200
SLPGAYLRSK GLNRIGNLLV PNDNYCKFED WIIPIFDEML KEQKEENVLW
210 220 230 240 250
TPSKLLARLG KEINNESSYL YWAYKMNIPV FCPGLTDGSL GDMLYFHSFR
260 270 280 290 300
TSGLIIDVVQ DIRAMNGEAV HANPKKTGMI ILGGGLPKHH ICNANMMRNG
310 320 330 340 350
ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL
360
LVAETFATKR DQTCESKT
Length:368
Mass (Da):41,064
Last modified:March 1, 2001 - v1
Checksum:i491D9DF362DAB002
GO

Sequence cautioni

The sequence AAK32840.1 differs from that shown. Reason: Frameshift at position 68. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291E → Q in AAG53642 (PubMed:11278418).Curated
Sequence conflicti67 – 671Missing in AAK32840 (PubMed:14593172).Curated
Sequence conflicti309 – 3091T → P in AAG53642 (PubMed:11278418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296078 mRNA. Translation: AAG53642.2.
AB017060 Genomic DNA. Translation: BAB10797.1.
CP002688 Genomic DNA. Translation: AED90939.1.
AF361828 mRNA. Translation: AAK32840.1. Frameshift.
AY056066 mRNA. Translation: AAL06966.1.
AY087829 mRNA. Translation: AAM65382.1.
RefSeqiNP_196211.1. NM_120674.3. [Q9FI94-1]
UniGeneiAt.9443.

Genome annotation databases

EnsemblPlantsiAT5G05920.1; AT5G05920.1; AT5G05920. [Q9FI94-1]
GeneIDi830477.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296078 mRNA. Translation: AAG53642.2.
AB017060 Genomic DNA. Translation: BAB10797.1.
CP002688 Genomic DNA. Translation: AED90939.1.
AF361828 mRNA. Translation: AAK32840.1. Frameshift.
AY056066 mRNA. Translation: AAL06966.1.
AY087829 mRNA. Translation: AAM65382.1.
RefSeqiNP_196211.1. NM_120674.3. [Q9FI94-1]
UniGeneiAt.9443.

3D structure databases

ProteinModelPortaliQ9FI94.
SMRiQ9FI94. Positions 27-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G05920.1.

PTM databases

iPTMnetiQ9FI94.

Proteomic databases

PaxDbiQ9FI94.
PRIDEiQ9FI94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G05920.1; AT5G05920.1; AT5G05920. [Q9FI94-1]
GeneIDi830477.

Organism-specific databases

TAIRiAT5G05920.

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
HOGENOMiHOG000228838.
InParanoidiQ9FI94.
OMAiSWGKIKE.
PhylomeDBiQ9FI94.

Enzyme and pathway databases

UniPathwayiUPA00354.
BioCyciARA:AT5G05920-MONOMER.
ARA:GQT-45-MONOMER.
ReactomeiR-ATH-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ9FI94.

Gene expression databases

ExpressionAtlasiQ9FI94. baseline.
GenevisibleiQ9FI94. AT.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato."
    Wang T.-W., Lu L., Wang D., Thompson J.E.
    J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiDHYS_ARATH
AccessioniPrimary (citable) accession number: Q9FI94
Secondary accession number(s): Q9ASU5, Q9C5Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: March 1, 2001
Last modified: February 17, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.