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Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136NADBy similarity1
Binding sitei237NADBy similarity1
Binding sitei242SpermidineBy similarity1
Binding sitei284NAD; via amide nitrogenBy similarity1
Binding sitei289SpermidineBy similarity1
Active sitei330NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 108NADBy similarity5
Nucleotide bindingi130 – 132NADBy similarity3
Nucleotide bindingi309 – 310NADBy similarity2
Nucleotide bindingi343 – 344NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT5G05920-MONOMER.
ReactomeiR-ATH-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
Ordered Locus Names:At5g05920
ORF Names:K18J17_7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G05920.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344731 – 368Deoxyhypusine synthaseAdd BLAST368

Proteomic databases

PaxDbiQ9FI94.
PRIDEiQ9FI94.

PTM databases

iPTMnetiQ9FI94.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FI94. differential.
GenevisibleiQ9FI94. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05920.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FI94.
SMRiQ9FI94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 136Spermidine bindingBy similarity2
Regioni315 – 317Spermidine bindingBy similarity3
Regioni324 – 330Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
HOGENOMiHOG000228838.
InParanoidiQ9FI94.
KOiK00809.
OMAiTTAVPWD.
OrthoDBiEOG09360CE8.
PhylomeDBiQ9FI94.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FI94-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDDRVFSSV HSTVFKESES LEGKCDKIEG YDFNQGVDYP KLMRSMLTTG
60 70 80 90 100
FQASNLGEAI DVVNQMLDWR LADETTVAED CSEEEKNPSF RESVKCKIFL
110 120 130 140 150
GFTSNLVSSG VRDTIRYLVQ HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF
160 170 180 190 200
SLPGAYLRSK GLNRIGNLLV PNDNYCKFED WIIPIFDEML KEQKEENVLW
210 220 230 240 250
TPSKLLARLG KEINNESSYL YWAYKMNIPV FCPGLTDGSL GDMLYFHSFR
260 270 280 290 300
TSGLIIDVVQ DIRAMNGEAV HANPKKTGMI ILGGGLPKHH ICNANMMRNG
310 320 330 340 350
ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL
360
LVAETFATKR DQTCESKT
Length:368
Mass (Da):41,064
Last modified:March 1, 2001 - v1
Checksum:i491D9DF362DAB002
GO

Sequence cautioni

The sequence AAK32840 differs from that shown. Reason: Frameshift at position 68.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29E → Q in AAG53642 (PubMed:11278418).Curated1
Sequence conflicti67Missing in AAK32840 (PubMed:14593172).Curated1
Sequence conflicti309T → P in AAG53642 (PubMed:11278418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296078 mRNA. Translation: AAG53642.2.
AB017060 Genomic DNA. Translation: BAB10797.1.
CP002688 Genomic DNA. Translation: AED90939.1.
AF361828 mRNA. Translation: AAK32840.1. Frameshift.
AY056066 mRNA. Translation: AAL06966.1.
AY087829 mRNA. Translation: AAM65382.1.
RefSeqiNP_196211.1. NM_120674.4. [Q9FI94-1]
UniGeneiAt.9443.

Genome annotation databases

EnsemblPlantsiAT5G05920.1; AT5G05920.1; AT5G05920. [Q9FI94-1]
GeneIDi830477.
GrameneiAT5G05920.1; AT5G05920.1; AT5G05920.
KEGGiath:AT5G05920.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296078 mRNA. Translation: AAG53642.2.
AB017060 Genomic DNA. Translation: BAB10797.1.
CP002688 Genomic DNA. Translation: AED90939.1.
AF361828 mRNA. Translation: AAK32840.1. Frameshift.
AY056066 mRNA. Translation: AAL06966.1.
AY087829 mRNA. Translation: AAM65382.1.
RefSeqiNP_196211.1. NM_120674.4. [Q9FI94-1]
UniGeneiAt.9443.

3D structure databases

ProteinModelPortaliQ9FI94.
SMRiQ9FI94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G05920.1.

PTM databases

iPTMnetiQ9FI94.

Proteomic databases

PaxDbiQ9FI94.
PRIDEiQ9FI94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G05920.1; AT5G05920.1; AT5G05920. [Q9FI94-1]
GeneIDi830477.
GrameneiAT5G05920.1; AT5G05920.1; AT5G05920.
KEGGiath:AT5G05920.

Organism-specific databases

TAIRiAT5G05920.

Phylogenomic databases

eggNOGiKOG2924. Eukaryota.
COG1899. LUCA.
HOGENOMiHOG000228838.
InParanoidiQ9FI94.
KOiK00809.
OMAiTTAVPWD.
OrthoDBiEOG09360CE8.
PhylomeDBiQ9FI94.

Enzyme and pathway databases

UniPathwayiUPA00354.
BioCyciARA:AT5G05920-MONOMER.
ReactomeiR-ATH-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ9FI94.

Gene expression databases

ExpressionAtlasiQ9FI94. differential.
GenevisibleiQ9FI94. AT.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
InterProiIPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHYS_ARATH
AccessioniPrimary (citable) accession number: Q9FI94
Secondary accession number(s): Q9ASU5, Q9C5Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.