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Q9FI61

- UBC27_ARATH

UniProt

Q9FI61 - UBC27_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 27

Gene

UBC27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G50870-MONOMER.
ARA:GQT-2083-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 27 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 27
Gene namesi
Name:UBC27
Synonyms:UBC1
Ordered Locus Names:At5g50870
ORF Names:K3K7.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G50870.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Ubiquitin-conjugating enzyme E2 27PRO_0000345192Add
BLAST

Proteomic databases

PaxDbiQ9FI61.
PRIDEiQ9FI61.

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Inductioni

Up-regulated by syringolin, a cell death-inducing chemical.1 Publication

Gene expression databases

ExpressionAtlasiQ9FI61. baseline.
GenevestigatoriQ9FI61.

Interactioni

Protein-protein interaction databases

BioGridi20405. 1 interaction.
IntActiQ9FI61. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9FI61.
SMRiQ9FI61. Positions 2-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 19240UBAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ9FI61.
KOiK04649.
PhylomeDBiQ9FI61.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FI61) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIDFSRIQKE LQDCERNQDS SGIRVCPKSD NLTRLTGTIP GPIGTPYEGG
60 70 80 90 100
TFQIDITMPD GYPFEPPKMQ FSTKVWHPNI SSQSGAICLD ILKDQWSPAL
110 120 130 140 150
TLKTALVSIQ ALLSAPEPKD PQDAVVAEQY MKNYQVFVST ARYWTETFAK
160 170 180 190
KSSLEEKVKR LVEMGFGDAQ VRSAIESSGG DENLALEKLC SA
Length:192
Mass (Da):21,254
Last modified:March 1, 2001 - v1
Checksum:i154FF8B581059646
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027040 mRNA. Translation: AAY44866.1.
DQ677667 mRNA. Translation: ABG85247.1.
AB017063 Genomic DNA. Translation: BAB08733.1.
CP002688 Genomic DNA. Translation: AED96003.1.
AY065303 mRNA. Translation: AAL38779.1.
AY096429 mRNA. Translation: AAM20069.1.
Z25704 mRNA. Translation: CAA81015.1.
RefSeqiNP_199900.1. NM_124465.5. [Q9FI61-1]
UniGeneiAt.6957.

Genome annotation databases

EnsemblPlantsiAT5G50870.1; AT5G50870.1; AT5G50870. [Q9FI61-1]
GeneIDi835159.
KEGGiath:AT5G50870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027040 mRNA. Translation: AAY44866.1 .
DQ677667 mRNA. Translation: ABG85247.1 .
AB017063 Genomic DNA. Translation: BAB08733.1 .
CP002688 Genomic DNA. Translation: AED96003.1 .
AY065303 mRNA. Translation: AAL38779.1 .
AY096429 mRNA. Translation: AAM20069.1 .
Z25704 mRNA. Translation: CAA81015.1 .
RefSeqi NP_199900.1. NM_124465.5. [Q9FI61-1 ]
UniGenei At.6957.

3D structure databases

ProteinModelPortali Q9FI61.
SMRi Q9FI61. Positions 2-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20405. 1 interaction.
IntActi Q9FI61. 1 interaction.

Proteomic databases

PaxDbi Q9FI61.
PRIDEi Q9FI61.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G50870.1 ; AT5G50870.1 ; AT5G50870 . [Q9FI61-1 ]
GeneIDi 835159.
KEGGi ath:AT5G50870.

Organism-specific databases

TAIRi AT5G50870.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
InParanoidi Q9FI61.
KOi K04649.
PhylomeDBi Q9FI61.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G50870-MONOMER.
ARA:GQT-2083-MONOMER.

Miscellaneous databases

PROi Q9FI61.

Gene expression databases

ExpressionAtlasi Q9FI61. baseline.
Genevestigatori Q9FI61.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view ]
SMARTi SM00165. UBA. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEi PS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
  2. "Functional differentiation of ubiquitin-interacting factors from Arabidopsis."
    Fu H.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The Arabidopsis thaliana transcribed genome: the GDR cDNA program."
    Hoefte H.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-192.
    Strain: cv. Columbia.
    Tissue: Seedling.

Entry informationi

Entry nameiUBC27_ARATH
AccessioniPrimary (citable) accession number: Q9FI61
Secondary accession number(s): Q42045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3