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Protein

Ubiquitin-conjugating enzyme E2 27

Gene

UBC27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 27 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 27
Ubiquitin carrier protein 27
Gene namesi
Name:UBC27
Synonyms:UBC1
Ordered Locus Names:At5g50870
ORF Names:K3K7.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G50870.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451921 – 192Ubiquitin-conjugating enzyme E2 27Add BLAST192

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Inductioni

Up-regulated by syringolin, a cell death-inducing chemical.1 Publication

Gene expression databases

ExpressionAtlasiQ9FI61. baseline and differential.
GenevisibleiQ9FI61. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20405. 1 interactor.
IntActiQ9FI61. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ9FI61.
SMRiQ9FI61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 192UBAPROSITE-ProRule annotationAdd BLAST40

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233455.
InParanoidiQ9FI61.
KOiK04649.
PhylomeDBiQ9FI61.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FI61-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIDFSRIQKE LQDCERNQDS SGIRVCPKSD NLTRLTGTIP GPIGTPYEGG
60 70 80 90 100
TFQIDITMPD GYPFEPPKMQ FSTKVWHPNI SSQSGAICLD ILKDQWSPAL
110 120 130 140 150
TLKTALVSIQ ALLSAPEPKD PQDAVVAEQY MKNYQVFVST ARYWTETFAK
160 170 180 190
KSSLEEKVKR LVEMGFGDAQ VRSAIESSGG DENLALEKLC SA
Length:192
Mass (Da):21,254
Last modified:March 1, 2001 - v1
Checksum:i154FF8B581059646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027040 mRNA. Translation: AAY44866.1.
DQ677667 mRNA. Translation: ABG85247.1.
AB017063 Genomic DNA. Translation: BAB08733.1.
CP002688 Genomic DNA. Translation: AED96003.1.
AY065303 mRNA. Translation: AAL38779.1.
AY096429 mRNA. Translation: AAM20069.1.
Z25704 mRNA. Translation: CAA81015.1.
RefSeqiNP_199900.1. NM_124465.6. [Q9FI61-1]
UniGeneiAt.6957.

Genome annotation databases

EnsemblPlantsiAT5G50870.1; AT5G50870.1; AT5G50870. [Q9FI61-1]
GeneIDi835159.
GrameneiAT5G50870.1; AT5G50870.1; AT5G50870.
KEGGiath:AT5G50870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027040 mRNA. Translation: AAY44866.1.
DQ677667 mRNA. Translation: ABG85247.1.
AB017063 Genomic DNA. Translation: BAB08733.1.
CP002688 Genomic DNA. Translation: AED96003.1.
AY065303 mRNA. Translation: AAL38779.1.
AY096429 mRNA. Translation: AAM20069.1.
Z25704 mRNA. Translation: CAA81015.1.
RefSeqiNP_199900.1. NM_124465.6. [Q9FI61-1]
UniGeneiAt.6957.

3D structure databases

ProteinModelPortaliQ9FI61.
SMRiQ9FI61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20405. 1 interactor.
IntActiQ9FI61. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G50870.1; AT5G50870.1; AT5G50870. [Q9FI61-1]
GeneIDi835159.
GrameneiAT5G50870.1; AT5G50870.1; AT5G50870.
KEGGiath:AT5G50870.

Organism-specific databases

TAIRiAT5G50870.

Phylogenomic databases

HOGENOMiHOG000233455.
InParanoidiQ9FI61.
KOiK04649.
PhylomeDBiQ9FI61.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

PROiQ9FI61.

Gene expression databases

ExpressionAtlasiQ9FI61. baseline and differential.
GenevisibleiQ9FI61. AT.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC27_ARATH
AccessioniPrimary (citable) accession number: Q9FI61
Secondary accession number(s): Q42045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.