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Protein

Ubiquitin-conjugating enzyme E2 27

Gene

UBC27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 27 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 27
Ubiquitin carrier protein 27
Gene namesi
Name:UBC27
Synonyms:UBC1
Ordered Locus Names:At5g50870
ORF Names:K3K7.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G50870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451921 – 192Ubiquitin-conjugating enzyme E2 27Add BLAST192

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Inductioni

Up-regulated by syringolin, a cell death-inducing chemical.1 Publication

Gene expression databases

ExpressionAtlasiQ9FI61 baseline and differential
GenevisibleiQ9FI61 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20405, 2 interactors
IntActiQ9FI61, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliQ9FI61
SMRiQ9FI61
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 192UBAPROSITE-ProRule annotationAdd BLAST40

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233455
InParanoidiQ9FI61
KOiK04649
PhylomeDBiQ9FI61

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR015940 UBA
IPR009060 UBA-like_sf
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00627 UBA, 1 hit
PF00179 UQ_con, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FI61-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIDFSRIQKE LQDCERNQDS SGIRVCPKSD NLTRLTGTIP GPIGTPYEGG
60 70 80 90 100
TFQIDITMPD GYPFEPPKMQ FSTKVWHPNI SSQSGAICLD ILKDQWSPAL
110 120 130 140 150
TLKTALVSIQ ALLSAPEPKD PQDAVVAEQY MKNYQVFVST ARYWTETFAK
160 170 180 190
KSSLEEKVKR LVEMGFGDAQ VRSAIESSGG DENLALEKLC SA
Length:192
Mass (Da):21,254
Last modified:March 1, 2001 - v1
Checksum:i154FF8B581059646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027040 mRNA Translation: AAY44866.1
DQ677667 mRNA Translation: ABG85247.1
AB017063 Genomic DNA Translation: BAB08733.1
CP002688 Genomic DNA Translation: AED96003.1
AY065303 mRNA Translation: AAL38779.1
AY096429 mRNA Translation: AAM20069.1
Z25704 mRNA Translation: CAA81015.1
RefSeqiNP_199900.1, NM_124465.6 [Q9FI61-1]
UniGeneiAt.6957

Genome annotation databases

EnsemblPlantsiAT5G50870.1; AT5G50870.1; AT5G50870 [Q9FI61-1]
GeneIDi835159
GrameneiAT5G50870.1; AT5G50870.1; AT5G50870 [Q9FI61-1]
KEGGiath:AT5G50870

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC27_ARATH
AccessioniPrimary (citable) accession number: Q9FI61
Secondary accession number(s): Q42045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health