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Q9FI61

- UBC27_ARATH

UniProt

Q9FI61 - UBC27_ARATH

Protein

Ubiquitin-conjugating enzyme E2 27

Gene

UBC27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G50870-MONOMER.
    ARA:GQT-2083-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 27 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 27
    Gene namesi
    Name:UBC27
    Synonyms:UBC1
    Ordered Locus Names:At5g50870
    ORF Names:K3K7.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G50870.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 192192Ubiquitin-conjugating enzyme E2 27PRO_0000345192Add
    BLAST

    Proteomic databases

    PaxDbiQ9FI61.
    PRIDEiQ9FI61.

    Expressioni

    Tissue specificityi

    Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

    Inductioni

    Up-regulated by syringolin, a cell death-inducing chemical.1 Publication

    Gene expression databases

    GenevestigatoriQ9FI61.

    Interactioni

    Protein-protein interaction databases

    BioGridi20405. 1 interaction.
    IntActiQ9FI61. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FI61.
    SMRiQ9FI61. Positions 2-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 19240UBAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiQ9FI61.
    KOiK04649.
    PhylomeDBiQ9FI61.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00627. UBA. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view]
    SMARTiSM00165. UBA. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEiPS50030. UBA. 1 hit.
    PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FI61-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIDFSRIQKE LQDCERNQDS SGIRVCPKSD NLTRLTGTIP GPIGTPYEGG    50
    TFQIDITMPD GYPFEPPKMQ FSTKVWHPNI SSQSGAICLD ILKDQWSPAL 100
    TLKTALVSIQ ALLSAPEPKD PQDAVVAEQY MKNYQVFVST ARYWTETFAK 150
    KSSLEEKVKR LVEMGFGDAQ VRSAIESSGG DENLALEKLC SA 192
    Length:192
    Mass (Da):21,254
    Last modified:March 1, 2001 - v1
    Checksum:i154FF8B581059646
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027040 mRNA. Translation: AAY44866.1.
    DQ677667 mRNA. Translation: ABG85247.1.
    AB017063 Genomic DNA. Translation: BAB08733.1.
    CP002688 Genomic DNA. Translation: AED96003.1.
    AY065303 mRNA. Translation: AAL38779.1.
    AY096429 mRNA. Translation: AAM20069.1.
    Z25704 mRNA. Translation: CAA81015.1.
    RefSeqiNP_199900.1. NM_124465.5. [Q9FI61-1]
    UniGeneiAt.6957.

    Genome annotation databases

    EnsemblPlantsiAT5G50870.1; AT5G50870.1; AT5G50870. [Q9FI61-1]
    GeneIDi835159.
    KEGGiath:AT5G50870.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027040 mRNA. Translation: AAY44866.1 .
    DQ677667 mRNA. Translation: ABG85247.1 .
    AB017063 Genomic DNA. Translation: BAB08733.1 .
    CP002688 Genomic DNA. Translation: AED96003.1 .
    AY065303 mRNA. Translation: AAL38779.1 .
    AY096429 mRNA. Translation: AAM20069.1 .
    Z25704 mRNA. Translation: CAA81015.1 .
    RefSeqi NP_199900.1. NM_124465.5. [Q9FI61-1 ]
    UniGenei At.6957.

    3D structure databases

    ProteinModelPortali Q9FI61.
    SMRi Q9FI61. Positions 2-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20405. 1 interaction.
    IntActi Q9FI61. 1 interaction.

    Proteomic databases

    PaxDbi Q9FI61.
    PRIDEi Q9FI61.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G50870.1 ; AT5G50870.1 ; AT5G50870 . [Q9FI61-1 ]
    GeneIDi 835159.
    KEGGi ath:AT5G50870.

    Organism-specific databases

    TAIRi AT5G50870.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi Q9FI61.
    KOi K04649.
    PhylomeDBi Q9FI61.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G50870-MONOMER.
    ARA:GQT-2083-MONOMER.

    Miscellaneous databases

    PROi Q9FI61.

    Gene expression databases

    Genevestigatori Q9FI61.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00627. UBA. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SMARTi SM00165. UBA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEi PS50030. UBA. 1 hit.
    PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
    2. "Functional differentiation of ubiquitin-interacting factors from Arabidopsis."
      Fu H.
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
      DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The Arabidopsis thaliana transcribed genome: the GDR cDNA program."
      Hoefte H.
      Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-192.
      Strain: cv. Columbia.
      Tissue: Seedling.

    Entry informationi

    Entry nameiUBC27_ARATH
    AccessioniPrimary (citable) accession number: Q9FI61
    Secondary accession number(s): Q42045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3