Q9FI61 (UBC27_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 27
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 27

Gene names

Name:	UBC27
Synonyms:	UBC1
Ordered Locus Names:	At5g50870
ORF Names:	K3K7.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Expressed in seeds, pistils, siliques, hypocotyls and leaves. Ref.1
Induction	Up-regulated by syringolin, a cell death-inducing chemical. Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family. Contains 1 UBA domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FI61-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Ubiquitin-conjugating enzyme E2 27		PRO_0000345192
Regions
	Domain	153 – 192	40	UBA
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 154FF8B581059646	FASTA	192	21,254
	10 20 30 40 50 60 MIDFSRIQKE LQDCERNQDS SGIRVCPKSD NLTRLTGTIP GPIGTPYEGG TFQIDITMPD 70 80 90 100 110 120 GYPFEPPKMQ FSTKVWHPNI SSQSGAICLD ILKDQWSPAL TLKTALVSIQ ALLSAPEPKD 130 140 150 160 170 180 PQDAVVAEQY MKNYQVFVST ARYWTETFAK KSSLEEKVKR LVEMGFGDAQ VRSAIESSGG 190 DENLALEKLC SA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
[2]	"Functional differentiation of ubiquitin-interacting factors from Arabidopsis." Fu H. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"The Arabidopsis thaliana transcribed genome: the GDR cDNA program." Hoefte H. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-192. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ027040 mRNA. Translation: AAY44866.1. DQ677667 mRNA. Translation: ABG85247.1. AB017063 Genomic DNA. Translation: BAB08733.1. CP002688 Genomic DNA. Translation: AED96003.1. AY065303 mRNA. Translation: AAL38779.1. AY096429 mRNA. Translation: AAM20069.1. Z25704 mRNA. Translation: CAA81015.1.
IPI	IPI00532771.
RefSeq	NP_199900.1. NM_124465.5.
UniGene	At.6957.
3D structure databases
HSSP	HSSP built from PDB template 2BEP based on UniProtKB P61085.
ProteinModelPortal	Q9FI61.
SMR	Q9FI61. Positions 2-191.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FI61. 1 interaction.
Proteomic databases
PaxDb	Q9FI61.
PRIDE	Q9FI61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G50870.1; AT5G50870.1; AT5G50870.
GeneID	835159.
KEGG	ath:AT5G50870.
Organism-specific databases
TAIR	At5g50870.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
InParanoid	Q9FI61.
KO	K04649.
PhylomeDB	Q9FI61.
ProtClustDB	CLSN2687200.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9FI61.
Genevestigator	Q9FI61.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. IPR015940. UBA/transl_elong_EF1B_N_euk. IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00627. UBA. 1 hit. PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00165. UBA. 1 hit. [Graphical view]
SUPFAM	SSF46934. UBA_like. 1 hit. SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS50030. UBA. 1 hit. PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC27_ARATH

Accession

Primary (citable) accession number: Q9FI61
Secondary accession number(s): Q42045

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program