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Protein

Chaperone protein ClpC1, chloroplastic

Gene

CLPC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that hydrolyzes ATP and is associated with the chloroplast protein import apparatus. May function as the motor for chloroplast protein translocation, as translocation requires ATP hydrolysis in the stroma. May interact with a ClpP-like protease involved in degradation of denatured proteins in the chloroplast. Involved in the regulation of chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase (CAO) protein in response to the accumulation of chlorophyll b. Involved in leaf iron homeostasis.8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi302 – 309ATPSequence analysis8
Nucleotide bindingi645 – 652ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent peptidase activity Source: TAIR

GO - Biological processi

  • chloroplast organization Source: TAIR
  • protein import into chloroplast stroma Source: TAIR
  • protein targeting to chloroplast Source: TAIR
  • regulation of chlorophyll biosynthetic process Source: TAIR
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transport
LigandATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.9.1.2 the chloroplast envelope protein translocase (cept or tic-toc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpC1, chloroplastic1 Publication
Alternative name(s):
ATP-dependent Clp protease ATP-binding subunit ClpC homolog 1
Casein lytic proteinase C1
Protein DE-REGULATED CAO ACCUMULATION 1
Protein IRON-RESCUED MUTANT 1
Gene namesi
Name:CLPC11 Publication
Synonyms:DCA1, HSP93-V1 Publication, IRM1
Ordered Locus Names:At5g50920Imported
ORF Names:K3K7.7Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT5G50920
TAIRilocus:2157383 AT5G50920

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

Small plants with chlorotic leaves, aberrant chloroplast biogenesis and inefficient chloroplast import of both photosynthetic and non-photosynthetic preproteins (PubMed:15516497, PubMed:15563614, Ref. 11, PubMed:15659100, PubMed:17376159, PubMed:17291312, PubMed:20382967). Clpc1 and clpc2 double mutants are embryo lethal when homozygous (PubMed:17376159).7 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi773G → R in irm1; iron deficiency chlorosis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38ChloroplastSequence analysisAdd BLAST38
ChainiPRO_000041257539 – 929Chaperone protein ClpC1, chloroplasticAdd BLAST891

Proteomic databases

PaxDbiQ9FI56
PRIDEiQ9FI56

Expressioni

Tissue specificityi

Highly expressed in rosette leaves. Expressed in roots, stems and inflorescences (PubMed:11982939, PubMed:15659100, PubMed:20382967). Expressed in photosynthetic green tissues with high levels in young, developing leaf tissues (PubMed:23898032).4 Publications

Inductioni

By cold and salt stresses. Not induced by heat stress.2 Publications

Gene expression databases

ExpressionAtlasiQ9FI56 baseline and differential
GenevisibleiQ9FI56 AT

Interactioni

Subunit structurei

Homodimer (PubMed:14593120). May form hexamer and interact with Clp core (PubMed:14593120). Interacts (via N-terminus) with CLPS1 (PubMed:23898032). Interacts with CLPF (PubMed:26419670).3 Publications

Protein-protein interaction databases

BioGridi20411, 2 interactors
IntActiQ9FI56, 1 interactor
STRINGi3702.AT5G50920.1

Structurei

Secondary structure

1929
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi100 – 115Combined sources16
Helixi123 – 132Combined sources10
Helixi137 – 144Combined sources8
Helixi149 – 160Combined sources12
Helixi175 – 190Combined sources16
Beta strandi194 – 196Combined sources3
Helixi198 – 208Combined sources11
Helixi212 – 219Combined sources8
Helixi224 – 237Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GUIX-ray1.20A94-238[»]
ProteinModelPortaliQ9FI56
SMRiQ9FI56
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini511 – 546UVRPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni257 – 504IBy similarityAdd BLAST248
Regioni571 – 762IIBy similarityAdd BLAST192

Domaini

The N-terminal domain (91-234) is important for membrane association and is essential for the in vivo functions, but not for the ATPase activity.1 Publication

Sequence similaritiesi

Belongs to the ClpA/ClpB family. ClpC subfamily.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1051 Eukaryota
COG0542 LUCA
HOGENOMiHOG000218210
InParanoidiQ9FI56
KOiK03696
OMAiFHSKVRQ
OrthoDBiEOG09360210
PhylomeDBiQ9FI56

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
IPR001943 UVR_dom
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PF02151 UVR, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit
PS50151 UVR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FI56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMATRVLAQ STPPSLACYQ RNVPSRGSGR SRRSVKMMCS QLQVSGLRMQ
60 70 80 90 100
GFMGLRGNNA LDTLGKSRQD FHSKVRQAMN VPKGKASRFT VKAMFERFTE
110 120 130 140 150
KAIKVIMLAQ EEARRLGHNF VGTEQILLGL IGEGTGIAAK VLKSMGINLK
160 170 180 190 200
DARVEVEKII GRGSGFVAVE IPFTPRAKRV LELSLEEARQ LGHNYIGSEH
210 220 230 240 250
LLLGLLREGE GVAARVLENL GADPSNIRTQ VIRMVGENNE VTANVGGGSS
260 270 280 290 300
SNKMPTLEEY GTNLTKLAEE GKLDPVVGRQ PQIERVVQIL GRRTKNNPCL
310 320 330 340 350
IGEPGVGKTA IAEGLAQRIA SGDVPETIEG KKVITLDMGL LVAGTKYRGE
360 370 380 390 400
FEERLKKLME EIRQSDEIIL FIDEVHTLIG AGAAEGAIDA ANILKPALAR
410 420 430 440 450
GELQCIGATT LDEYRKHIEK DPALERRFQP VKVPEPTVDE TIQILKGLRE
460 470 480 490 500
RYEIHHKLRY TDESLVAAAQ LSYQYISDRF LPDKAIDLID EAGSRVRLRH
510 520 530 540 550
AQVPEEAREL EKELRQITKE KNEAVRGQDF EKAGTLRDRE IELRAEVSAI
560 570 580 590 600
QAKGKEMSKA ESETGEEGPM VTESDIQHIV SSWTGIPVEK VSTDESDRLL
610 620 630 640 650
KMEETLHKRI IGQDEAVKAI SRAIRRARVG LKNPNRPIAS FIFSGPTGVG
660 670 680 690 700
KSELAKALAA YYFGSEEAMI RLDMSEFMER HTVSKLIGSP PGYVGYTEGG
710 720 730 740 750
QLTEAVRRRP YTVVLFDEIE KAHPDVFNMM LQILEDGRLT DSKGRTVDFK
760 770 780 790 800
NTLLIMTSNV GSSVIEKGGR RIGFDLDYDE KDSSYNRIKS LVTEELKQYF
810 820 830 840 850
RPEFLNRLDE MIVFRQLTKL EVKEIADILL KEVFERLKKK EIELQVTERF
860 870 880 890 900
KERVVDEGYN PSYGARPLRR AIMRLLEDSM AEKMLAREIK EGDSVIVDVD
910 920
AEGNVTVLNG GSGTPTTSLE EQEDSLPVA
Length:929
Mass (Da):103,453
Last modified:March 1, 2001 - v1
Checksum:i438DEA514125F0BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40 – 48SQLQVSGLR → IIFNVWLP in AAC04687 (PubMed:11982939).Curated9
Sequence conflicti187 – 188EA → AT in AAC04687 (PubMed:11982939).Curated2
Sequence conflicti507A → V in AAC04687 (PubMed:11982939).Curated1
Sequence conflicti685K → T in AAC04687 (PubMed:11982939).Curated1
Sequence conflicti690P → L in AAC04687 (PubMed:11982939).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022909 mRNA Translation: AAC04687.1
AB017063 Genomic DNA Translation: BAB08738.1
CP002688 Genomic DNA Translation: AED96011.1
AY102125 mRNA Translation: AAM26692.1
AK227173 mRNA Translation: BAE99213.1
PIRiT52292
RefSeqiNP_568746.1, NM_124471.4
UniGeneiAt.24774
At.74761
At.74764
At.75059

Genome annotation databases

EnsemblPlantsiAT5G50920.1; AT5G50920.1; AT5G50920
GeneIDi835165
GrameneiAT5G50920.1; AT5G50920.1; AT5G50920
KEGGiath:AT5G50920

Entry informationi

Entry nameiCLPC1_ARATH
AccessioniPrimary (citable) accession number: Q9FI56
Secondary accession number(s): O48931
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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