Q9FI53 (FUM2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase 2, chloroplastic
Short name=Fumarase 2
EC=4.2.1.2

Gene names

Name:	FUM2
Ordered Locus Names:	At5g50950
ORF Names:	K3K7.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Subcellular location	Plastid › chloroplast Potential.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro nitrate assimilation Inferred from mutant phenotype PubMed 20202172. Source: TAIR response to salt stress Inferred from expression pattern PubMed 17916636. Source: TAIR tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay PubMed 20202172. Source: TAIR mitochondrion Inferred from direct assay PubMed 11743115PubMed 14671022. Source: TAIR tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular_function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FI53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FI53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     472-499: HAAMKLGVLTSEEFDTLVVPEKMIGPSD → VNNKLLTFSSLNKSEFKPIFSKRKHVHVCYNIFVVLFWI

Note: Derived from EST data. No experimental confirmation available.

Isoform 3 (identifier: Q9FI53-3)

The sequence of this isoform differs from the canonical sequence as follows:
     210-214: SFEFK → VTHLM
     215-499: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 499		Fumarate hydratase 2, chloroplastic		PRO_0000010330
Regions
	Region	164 – 167	4	B site By similarity
	Region	174 – 176	3	Substrate binding By similarity
Sites
	Binding site	136	1	Substrate By similarity
Natural variations
	Alternative sequence	210 – 214	5	SFEFK → VTHLM in isoform 3.		VSP_011356
	Alternative sequence	215 – 499	285	Missing in isoform 3.		VSP_011357
	Alternative sequence	472 – 499	28	HAAMK…IGPSD → VNNKLLTFSSLNKSEFKPIF SKRKHVHVCYNIFVVLFWI in isoform 2.		VSP_011355

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A84422EB032FCB4E	FASTA	499	54,084
	10 20 30 40 50 60 MAALTMQFEG EKKNVSEVAD VTLKQEDEQQ ERRSYSTPFR EERDTFGPIQ VPSDKLWGAQ 70 80 90 100 110 120 TQRSLQNFEI GGDRERMPEP IVRAFGVLKK CAAKVNMEYG LDPMIGEAIM EAAQEVAEGK 130 140 150 160 170 180 LNDHFPLVVW QTGSGTQSNM NANEVIANRA AEILGHKRGE KIVHPNDHVN RSQSSNDTFP 190 200 210 220 230 240 TVMHIAAATE ITSRLIPSLK NLHSSLESKS FEFKDIVKIG RTHTQDATPL TLGQEFGGYA 250 260 270 280 290 300 TQVEYGLNRV ACTLPRIYQL AQGGTAVGTG LNTKKGFDVK IAAAVAEETN LPFVTAENKF 310 320 330 340 350 360 EALAAHDACV ETSGSLNTIA TSLMKIANDI RFLGSGPRCG LGELSLPENE PGSSIMPGKV 370 380 390 400 410 420 NPTQCEALTM VCAQVMGNHV AVTIGGSNGH FELNVFKPVI ASALLHSIRL IADASASFEK 430 440 450 460 470 480 NCVRGIEANR ERISKLLHES LMLVTSLNPK IGYDNAAAVA KRAHKEGCTL KHAAMKLGVL 490 TSEEFDTLVV PEKMIGPSD
	Isoform 2 [UniParc]. Checksum: 4B192A14B3DD12D9	FASTA	510	55,766
	10 20 30 40 50 60 MAALTMQFEG EKKNVSEVAD VTLKQEDEQQ ERRSYSTPFR EERDTFGPIQ VPSDKLWGAQ 70 80 90 100 110 120 TQRSLQNFEI GGDRERMPEP IVRAFGVLKK CAAKVNMEYG LDPMIGEAIM EAAQEVAEGK 130 140 150 160 170 180 LNDHFPLVVW QTGSGTQSNM NANEVIANRA AEILGHKRGE KIVHPNDHVN RSQSSNDTFP 190 200 210 220 230 240 TVMHIAAATE ITSRLIPSLK NLHSSLESKS FEFKDIVKIG RTHTQDATPL TLGQEFGGYA 250 260 270 280 290 300 TQVEYGLNRV ACTLPRIYQL AQGGTAVGTG LNTKKGFDVK IAAAVAEETN LPFVTAENKF 310 320 330 340 350 360 EALAAHDACV ETSGSLNTIA TSLMKIANDI RFLGSGPRCG LGELSLPENE PGSSIMPGKV 370 380 390 400 410 420 NPTQCEALTM VCAQVMGNHV AVTIGGSNGH FELNVFKPVI ASALLHSIRL IADASASFEK 430 440 450 460 470 480 NCVRGIEANR ERISKLLHES LMLVTSLNPK IGYDNAAAVA KRAHKEGCTL KVNNKLLTFS 490 500 510 SLNKSEFKPI FSKRKHVHVC YNIFVVLFWI
	Isoform 3 [UniParc]. Checksum: B56D413A83C88E25	FASTA	214	23,885
	10 20 30 40 50 60 MAALTMQFEG EKKNVSEVAD VTLKQEDEQQ ERRSYSTPFR EERDTFGPIQ VPSDKLWGAQ 70 80 90 100 110 120 TQRSLQNFEI GGDRERMPEP IVRAFGVLKK CAAKVNMEYG LDPMIGEAIM EAAQEVAEGK 130 140 150 160 170 180 LNDHFPLVVW QTGSGTQSNM NANEVIANRA AEILGHKRGE KIVHPNDHVN RSQSSNDTFP 190 200 210 TVMHIAAATE ITSRLIPSLK NLHSSLESKV THLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017063 Genomic DNA. Translation: BAB08741.1. CP002688 Genomic DNA. Translation: AED96014.1. CP002688 Genomic DNA. Translation: AED96015.1. AY052197 mRNA. Translation: AAK97668.1. AY072393 mRNA. Translation: AAL62385.1. AY113070 mRNA. Translation: AAM47378.1.
IPI	IPI00525062. IPI00526175. IPI00546845.
RefSeq	NP_199908.1. NM_124474.3. NP_851166.1. NM_180835.2.
UniGene	At.6963.
3D structure databases
ProteinModelPortal	Q9FI53.
SMR	Q9FI53. Positions 39-497.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FI53. 1 interaction.
Proteomic databases
PaxDb	Q9FI53.
PRIDE	Q9FI53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G50950.2; AT5G50950.2; AT5G50950.
GeneID	835168.
KEGG	ath:AT5G50950.
Organism-specific databases
GeneFarm	4371. 440.
TAIR	At5g50950.
Phylogenomic databases
eggNOG	COG0114.
HOGENOM	HOG000061736.
InParanoid	Q9FI53.
KO	K01679.
OMA	SFRVHCV.
PhylomeDB	Q9FI53.
ProtClustDB	PLN00134.
Enzyme and pathway databases
BioCyc	ARA:AT5G50950-MONOMER. MetaCyc:AT5G50950-MONOMER.
UniPathway	UPA00223; UER01007.
Gene expression databases
Genevestigator	Q9FI53.
GermOnline	AT5G50950. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
InterPro	IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUM2_ARATH

Accession

Primary (citable) accession number: Q9FI53
Secondary accession number(s): Q8VY72

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program