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Reviewed, UniProtKB/Swiss-Prot Q9FI53 (FUM2_ARATH)

Last modified February 9, 2010. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Fumarate hydratase 2, chloroplastic
      Short name=Fumarase 2
    EC=4.2.1.2
Gene names
Name: FUM2
Ordered Locus Names: At5g50950
ORF Names: K3K7.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

Subcellular location

Plastidchloroplast Potential.

Miscellaneous

There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FI53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FI53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     472-499: HAAMKLGVLTSEEFDTLVVPEKMIGPSD → VNNKLLTFSSLNKSEFKPIFSKRKHVHVCYNIFVVLFWI
Note: Derived from EST data. No experimental confirmation available.
Isoform 3 (identifier: Q9FI53-3)

The sequence of this isoform differs from the canonical sequence as follows:
     210-214: SFEFK → VTHLM
     215-499: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 499Fumarate hydratase 2, chloroplasticPRO_0000010330

Regions

Region164 – 1674B site By similarity
Region174 – 1763Substrate binding By similarity

Sites

Binding site1361Substrate By similarity

Natural variations

Alternative sequence210 – 2145SFEFK → VTHLM in isoform 3.
VSP_011356
Alternative sequence215 – 499285Missing in isoform 3.
VSP_011357
Alternative sequence472 – 49928HAAMK…IGPSD → VNNKLLTFSSLNKSEFKPIF SKRKHVHVCYNIFVVLFWI in isoform 2.
VSP_011355

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: A84422EB032FCB4E

FASTA49954,084
        10         20         30         40         50         60 
MAALTMQFEG EKKNVSEVAD VTLKQEDEQQ ERRSYSTPFR EERDTFGPIQ VPSDKLWGAQ 

        70         80         90        100        110        120 
TQRSLQNFEI GGDRERMPEP IVRAFGVLKK CAAKVNMEYG LDPMIGEAIM EAAQEVAEGK 

       130        140        150        160        170        180 
LNDHFPLVVW QTGSGTQSNM NANEVIANRA AEILGHKRGE KIVHPNDHVN RSQSSNDTFP 

       190        200        210        220        230        240 
TVMHIAAATE ITSRLIPSLK NLHSSLESKS FEFKDIVKIG RTHTQDATPL TLGQEFGGYA 

       250        260        270        280        290        300 
TQVEYGLNRV ACTLPRIYQL AQGGTAVGTG LNTKKGFDVK IAAAVAEETN LPFVTAENKF 

       310        320        330        340        350        360 
EALAAHDACV ETSGSLNTIA TSLMKIANDI RFLGSGPRCG LGELSLPENE PGSSIMPGKV 

       370        380        390        400        410        420 
NPTQCEALTM VCAQVMGNHV AVTIGGSNGH FELNVFKPVI ASALLHSIRL IADASASFEK 

       430        440        450        460        470        480 
NCVRGIEANR ERISKLLHES LMLVTSLNPK IGYDNAAAVA KRAHKEGCTL KHAAMKLGVL 

       490 
TSEEFDTLVV PEKMIGPSD 

« Hide

Isoform 2.

Checksum: 4B192A14B3DD12D9
Show »

FASTA51055,766
Isoform 3.

Checksum: B56D413A83C88E25
Show »

FASTA21423,885

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017063 Genomic DNA. Translation: BAB08741.1.
AY052197 mRNA. Translation: AAK97668.1.
AY072393 mRNA. Translation: AAL62385.1.
AY113070 mRNA. Translation: AAM47378.1.
IPIIPI00525062.
IPI00526175.
IPI00546845.
RefSeqNP_199908.1.
UniGeneAt.6963

3D structure databases

SMRQ9FI53. Positions 39-497.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9FI53.

Proteomic databases

PRIDEQ9FI53.

Genome annotation databases

GeneID835168.
GenomeReviewsGene locus AT5G50950 in contig BA000015_GR.
NMPDRfig|3702.1.peg.26958.

Organism-specific databases

GeneFarm4371. 440.
TAIRAt5g50950.

Phylogenomic databases

eggNOGKOG1317.
HOGENOMHBG284369.
InParanoidQ9FI53.
OMAPANKFRG.
PhylomeDBQ9FI53.

Enzyme and pathway databases

BRENDA4.2.1.2. 302.

Gene expression databases

ArrayExpressQ9FI53.
GenevestigatorQ9FI53.
GermOnlineAT5G50950. Arabidopsis thaliana.

Family and domain databases

InterProIPR005677. Fum_hydII.
IPR018951. Fumarase_C_C.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
IPR008948. L-Aspartase-like.
[Graphical view]
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
TIGRFAMsTIGR00979. fumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFUM2_ARATH
AccessionPrimary (citable) accession number: Q9FI53
Secondary accession number(s): Q8VY72
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2001
Last modified: February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents