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Protein

Thalianol synthase

Gene

THAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts oxidosqualene to thalianol.2 Publications

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = thalianol.1 Publication

GO - Molecular functioni

  • thalianol synthase activity Source: TAIR

GO - Biological processi

  • root development Source: TAIR
  • thalianol metabolic process Source: TAIR
  • tricyclic triterpenoid biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Thalianol synthase (EC:5.4.99.31)
Short name:
AtTHAS1
Alternative name(s):
Pentacyclic triterpene synthase 4
Short name:
AtPEN4
Gene namesi
Name:THAS1
Synonyms:PEN4
Ordered Locus Names:At5g48010
ORF Names:MDN11.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G48010.

Pathology & Biotechi

Disruption phenotypei

Loss of thalianol production in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003661391 – 766Thalianol synthaseAdd BLAST766

Proteomic databases

PaxDbiQ9FI37.
PRIDEiQ9FI37.

Expressioni

Tissue specificityi

Expressed primarily in the root epidermis.1 Publication

Gene expression databases

GenevisibleiQ9FI37. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G48010.2.

Structurei

3D structure databases

ProteinModelPortaliQ9FI37.
SMRiQ9FI37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati149 – 190PFTB 1Add BLAST42
Repeati520 – 561PFTB 2Add BLAST42
Repeati646 – 687PFTB 3Add BLAST42

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 3 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ9FI37.
KOiK15821.
OMAiREYIWIG.
OrthoDBiEOG093611X6.
PhylomeDBiQ9FI37.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FI37-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRLRTGPKA GEDTHLFTTN NYAGRQIWEF DANAGSPQEI AEVEDARHKF
60 70 80 90 100
SDNTSRFKTT ADLLWRMQFL REKKFEQKIP RVIIEDARKI KYEDAKTALK
110 120 130 140 150
RGLLYFTALQ ADDGHWPAEN SGPNFYTPPF LICLYITGHL EKIFTPEHVK
160 170 180 190 200
ELLRHIYNMQ NEDGGWGLHV ESHSVMFCTV INYVCLRIVG EEVGHDDQRN
210 220 230 240 250
GCAKAHKWIM DHGGATYTPL IGKALLSVLG VYDWSGCNPI PPEFWLLPSS
260 270 280 290 300
FPVNGGTLWI YLRDTFMGLS YLYGKKFVAP PTPLILQLRE ELYPEPYAKI
310 320 330 340 350
NWTQTRNRCG KEDLYYPRSF LQDLFWKSVH MFSESILDRW PLNKLIRQRA
360 370 380 390 400
LQSTMALIHY HDESTRYITG GCLPKAFHML ACWIEDPKSD YFKKHLARVR
410 420 430 440 450
EYIWIGEDGL KIQSFGSQLW DTALSLHALL DGIDDHDVDD EIKTTLVKGY
460 470 480 490 500
DYLKKSQITE NPRGDHFKMF RHKTKGGWTF SDQDQGWPVS DCTAESLECC
510 520 530 540 550
LFFESMPSEL IGKKMDVEKL YDAVDYLLYL QSDNGGIAAW QPVEGKAWLE
560 570 580 590 600
WLSPVEFLED TIVEYEYVEC TGSAIAALTQ FNKQFPGYKN VEVKRFITKA
610 620 630 640 650
AKYIEDMQTV DGSWYGNWGV CFIYGTFFAV RGLVAAGKTY SNCEAIRKAV
660 670 680 690 700
RFLLDTQNPE GGWGESFLSC PSKKYTPLKG NSTNVVQTAQ ALMVLIMGDQ
710 720 730 740 750
MERDPLPVHR AAQVLINSQL DNGDFPQQEI MGTFMRTVML HFPTYRNTFS
760
LWALTHYTHA LRRLLP
Length:766
Mass (Da):88,203
Last modified:March 3, 2009 - v2
Checksum:i32D8D3E73A9E0B2C
GO
Isoform 2 (identifier: Q9FI37-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     551-566: WLSPVEFLEDTIVEYE → LLNIMIFR

Note: No experimental confirmation available.
Show »
Length:758
Mass (Da):87,253
Checksum:i93E1EBCD1F537444
GO

Sequence cautioni

The sequence BAB11065 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15H → R in BAE99758 (Ref. 4) Curated1
Sequence conflicti755T → A in BAE99758 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036570551 – 566WLSPV…IVEYE → LLNIMIFR in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY327541 mRNA. Translation: AAP92117.1.
AB017064 Genomic DNA. Translation: BAB11065.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95609.1.
CP002688 Genomic DNA. Translation: AED95610.1.
AK227776 mRNA. Translation: BAE99758.1.
RefSeqiNP_001078733.1. NM_001085264.2. [Q9FI37-1]
NP_199612.3. NM_124175.4. [Q9FI37-2]
UniGeneiAt.29870.

Genome annotation databases

EnsemblPlantsiAT5G48010.2; AT5G48010.2; AT5G48010. [Q9FI37-1]
GeneIDi834852.
GrameneiAT5G48010.2; AT5G48010.2; AT5G48010.
KEGGiath:AT5G48010.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY327541 mRNA. Translation: AAP92117.1.
AB017064 Genomic DNA. Translation: BAB11065.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95609.1.
CP002688 Genomic DNA. Translation: AED95610.1.
AK227776 mRNA. Translation: BAE99758.1.
RefSeqiNP_001078733.1. NM_001085264.2. [Q9FI37-1]
NP_199612.3. NM_124175.4. [Q9FI37-2]
UniGeneiAt.29870.

3D structure databases

ProteinModelPortaliQ9FI37.
SMRiQ9FI37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G48010.2.

Proteomic databases

PaxDbiQ9FI37.
PRIDEiQ9FI37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G48010.2; AT5G48010.2; AT5G48010. [Q9FI37-1]
GeneIDi834852.
GrameneiAT5G48010.2; AT5G48010.2; AT5G48010.
KEGGiath:AT5G48010.

Organism-specific databases

TAIRiAT5G48010.

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ9FI37.
KOiK15821.
OMAiREYIWIG.
OrthoDBiEOG093611X6.
PhylomeDBiQ9FI37.

Miscellaneous databases

PROiQ9FI37.

Gene expression databases

GenevisibleiQ9FI37. AT.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEN4_ARATH
AccessioniPrimary (citable) accession number: Q9FI37
Secondary accession number(s): Q0WSZ0, Q7XJ43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Constitutes with three contiguous genes an operon-like gene cluster that is involved in the thalianol pathway.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.