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Q9FI31

- XTH20_ARATH

UniProt

Q9FI31 - XTH20_ARATH

Protein

Xyloglucan endotransglucosylase/hydrolase protein 20

Gene

XTH20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei104 – 1041NucleophilePROSITE-ProRule annotation
    Active sitei108 – 1081Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT5G48070-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein 20 (EC:2.4.1.207)
    Short name:
    At-XTH20
    Short name:
    XTH-20
    Gene namesi
    Name:XTH20
    Ordered Locus Names:At5g48070
    ORF Names:MDN11.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G48070.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 282256Xyloglucan endotransglucosylase/hydrolase protein 20PRO_0000011820Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9FI31.

    Expressioni

    Tissue specificityi

    Root specific.1 Publication

    Gene expression databases

    GenevestigatoriQ9FI31.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FI31.
    SMRiQ9FI31. Positions 47-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiQ9FI31.
    KOiK08235.
    OMAiRFAFLII.
    PhylomeDBiQ9FI31.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FI31-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSFCGRRFA FLIIFLFAAQ YERVYAGSFH KDVQIHWGDG RGKILDNVGN    50
    LLSLSLDKFS GSGFQSHQEF LYGKVEVQMK LVPGNSAGTV TTFYLKSPGT 100
    TWDEIDFEFL GNISGHPYTL HTNVYTKGTG DKEQQFHLWF DPTVDFHTYC 150
    IIWNPQRVIF TIDGIPIREF KNSEALGVPF PKHQPMRLYA SLWEAEHWAT 200
    RGGLEKTDWS KAPFTAFYRN YNVDACVWSN GKSSCSANSS WFTQVLDFKG 250
    KNRVKWAQRK YMVYNYCTDK KRFPQGAPPE CS 282
    Length:282
    Mass (Da):32,437
    Last modified:March 1, 2001 - v1
    Checksum:iFBB8C587F6C9A3C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017064 Genomic DNA. Translation: BAB11071.1.
    CP002688 Genomic DNA. Translation: AED95616.1.
    BT012361 mRNA. Translation: AAS77486.1.
    AK221454 mRNA. Translation: BAD94531.1.
    RefSeqiNP_199618.1. NM_124181.3.
    UniGeneiAt.42985.

    Genome annotation databases

    EnsemblPlantsiAT5G48070.1; AT5G48070.1; AT5G48070.
    GeneIDi834859.
    KEGGiath:AT5G48070.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017064 Genomic DNA. Translation: BAB11071.1 .
    CP002688 Genomic DNA. Translation: AED95616.1 .
    BT012361 mRNA. Translation: AAS77486.1 .
    AK221454 mRNA. Translation: BAD94531.1 .
    RefSeqi NP_199618.1. NM_124181.3.
    UniGenei At.42985.

    3D structure databases

    ProteinModelPortali Q9FI31.
    SMRi Q9FI31. Positions 47-281.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PRIDEi Q9FI31.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G48070.1 ; AT5G48070.1 ; AT5G48070 .
    GeneIDi 834859.
    KEGGi ath:AT5G48070.

    Organism-specific databases

    TAIRi AT5G48070.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi Q9FI31.
    KOi K08235.
    OMAi RFAFLII.
    PhylomeDBi Q9FI31.

    Enzyme and pathway databases

    BioCyci ARA:AT5G48070-MONOMER.

    Gene expression databases

    Genevestigatori Q9FI31.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
      DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
      Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH20_ARATH
    AccessioniPrimary (citable) accession number: Q9FI31
    Secondary accession number(s): Q56Y69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3