Q9FI31 (XTH20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 20
Short name=At-XTH20
Short name=XTH-20
EC=2.4.1.207

Gene names

Name:	XTH20
Ordered Locus Names:	At5g48070
ORF Names:	MDN11.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Root specific. Ref.5
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 282	256	Xyloglucan endotransglucosylase/hydrolase protein 20		PRO_0000011820
Sites
	Active site	104	1	Nucleophile By similarity
	Active site	108	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FI31 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FBB8C587F6C9A3C3	FASTA	282	32,437
	10 20 30 40 50 60 MVSFCGRRFA FLIIFLFAAQ YERVYAGSFH KDVQIHWGDG RGKILDNVGN LLSLSLDKFS 70 80 90 100 110 120 GSGFQSHQEF LYGKVEVQMK LVPGNSAGTV TTFYLKSPGT TWDEIDFEFL GNISGHPYTL 130 140 150 160 170 180 HTNVYTKGTG DKEQQFHLWF DPTVDFHTYC IIWNPQRVIF TIDGIPIREF KNSEALGVPF 190 200 210 220 230 240 PKHQPMRLYA SLWEAEHWAT RGGLEKTDWS KAPFTAFYRN YNVDACVWSN GKSSCSANSS 250 260 270 280 WFTQVLDFKG KNRVKWAQRK YMVYNYCTDK KRFPQGAPPE CS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis." Yokoyama R., Nishitani K. Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature." Rose J.K.C., Braam J., Fry S.C., Nishitani K. Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

XTH-World

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017064 Genomic DNA. Translation: BAB11071.1. CP002688 Genomic DNA. Translation: AED95616.1. BT012361 mRNA. Translation: AAS77486.1. AK221454 mRNA. Translation: BAD94531.1.
IPI	IPI00545426.
RefSeq	NP_199618.1. NM_124181.3.
UniGene	At.42985.
3D structure databases
ProteinModelPortal	Q9FI31.
SMR	Q9FI31. Positions 47-281.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G48070.1; AT5G48070.1; AT5G48070.
GeneID	834859.
KEGG	ath:AT5G48070.
Organism-specific databases
TAIR	At5g48070.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
InParanoid	Q9FI31.
KO	K08235.
OMA	FTIDGIP.
PhylomeDB	Q9FI31.
ProtClustDB	CLSN2679615.
Gene expression databases
Genevestigator	Q9FI31.
GermOnline	AT5G48070. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008264. Beta_glucanase. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
PRINTS	PR00737. GLHYDRLASE16.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH20_ARATH

Accession

Primary (citable) accession number: Q9FI31
Secondary accession number(s): Q56Y69

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program