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Q9FHX5 (E1310_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase 10

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase 10
Short name=(1->3)-beta-glucanase 10
Beta-1,3-endoglucanase 10
Short name=Beta-1,3-glucanase 10
Gene names
Ordered Locus Names:At5g42100
ORF Names:MJC20.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Secretedcell wall Potential. Cell membrane; Lipid-anchorGPI-anchor; Extracellular side.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FHX5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FHX5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     404-423: GKGRFVECVLFFFLLCIIKL → VTFSTLHFICCFTLTIFVDI
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 401375Glucan endo-1,3-beta-glucosidase 10
PRO_0000251267
Propeptide402 – 42524Removed in mature form Potential
PRO_0000251268

Regions

Compositional bias370 – 3734Poly-Gly

Sites

Active site2661Nucleophile By similarity
Active site3301Nucleophile By similarity

Amino acid modifications

Lipidation4011GPI-anchor amidated serine Potential
Glycosylation1241N-linked (GlcNAc...) Potential
Glycosylation3621N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence404 – 42320GKGRF…CIIKL → VTFSTLHFICCFTLTIFVDI in isoform 2.
VSP_020752

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 972C1FC722408DA8

FASTA42545,358
        10         20         30         40         50         60 
MASSSLQSLF SLFCLALFSL PLIVSSIGIN YGQVANNLPP PKNVIPLLKS VGATKVKLYD 

        70         80         90        100        110        120 
ADPQALRAFA GSGFELTVAL GNEYLAQMSD PIKAQGWVKE NVQAYLPNTK IVAIVVGNEV 

       130        140        150        160        170        180 
LTSNQSALTA ALFPAMQSIH GALVDCGLNK QIFVTTAHSL AILDVSYPPS ATSFRRDLLG 

       190        200        210        220        230        240 
SLTPILDFHV KTGSPILINA YPFFAYEENP KHVSLDFVLF QPNQGFTDPG SNFHYDNMLF 

       250        260        270        280        290        300 
AQVDAVYHAL DAVGISYKKV PIVVSETGWP SNGDPQEVGA TCDNARKYNG NLIKMMMSKK 

       310        320        330        340        350        360 
MRTPIRPECD LTIFVFALFN ENMKPGPTSE RNYGLFNPDG TPVYSLGIKT SSTHSSGSGS 

       370        380        390        400        410        420 
SNSTGGSSSG GGGNTGGSSS GGGIYQPVTG NPSPDYMSIS SAGGKGRFVE CVLFFFLLCI 


IKLRL 

« Hide

Isoform 2 [UniParc].

Checksum: 12CEA28040320871
Show »

FASTA42545,333

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
[6]"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017067 Genomic DNA. Translation: BAB08443.1.
CP002688 Genomic DNA. Translation: AED94767.1.
BT008863 mRNA. Translation: AAP68302.1.
AY054690 mRNA. Translation: AAK96881.1.
AY084866 mRNA. Translation: AAM61429.1.
RefSeqNP_199025.1. NM_123575.3.
UniGeneAt.67156.
At.7792.

3D structure databases

ProteinModelPortalQ9FHX5.
SMRQ9FHX5. Positions 27-346.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbQ9FHX5.
PRIDEQ9FHX5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G42100.1; AT5G42100.1; AT5G42100. [Q9FHX5-1]
GeneID834215.
KEGGath:AT5G42100.

Organism-specific databases

TAIRAT5G42100.

Phylogenomic databases

eggNOGNOG328711.
HOGENOMHOG000238220.
InParanoidQ9FHX5.
OMAILDYHAR.
PhylomeDBQ9FHX5.
ProtClustDBCLSN2680350.

Enzyme and pathway databases

BioCycARA:AT5G42100-MONOMER.
ARA:GQT-196-MONOMER.

Gene expression databases

GenevestigatorQ9FHX5.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE1310_ARATH
AccessionPrimary (citable) accession number: Q9FHX5
Secondary accession number(s): Q3E8I9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names