Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9FHX5

- E1310_ARATH

UniProt

Q9FHX5 - E1310_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 10

Gene

At5g42100

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei266 – 2661NucleophileBy similarity
    Active sitei330 – 3301NucleophileBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell communication Source: TAIR
    3. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT5G42100-MONOMER.
    ARA:GQT-196-MONOMER.

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 10 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 10
    Short name:
    (1->3)-beta-glucanase 10
    Beta-1,3-endoglucanase 10
    Short name:
    Beta-1,3-glucanase 10
    Gene namesi
    Ordered Locus Names:At5g42100
    ORF Names:MJC20.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G42100.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: TAIR
    2. anchored component of plasma membrane Source: TAIR
    3. cell wall Source: TAIR
    4. endoplasmic reticulum Source: TAIR
    5. extracellular region Source: UniProtKB-KW
    6. plant-type cell wall Source: TAIR
    7. plasma membrane Source: TAIR
    8. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 401375Glucan endo-1,3-beta-glucosidase 10PRO_0000251267Add
    BLAST
    Propeptidei402 – 42524Removed in mature formSequence AnalysisPRO_0000251268Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis
    Lipidationi401 – 4011GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9FHX5.
    PRIDEiQ9FHX5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FHX5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FHX5.
    SMRiQ9FHX5. Positions 27-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi370 – 3734Poly-Gly

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG328711.
    HOGENOMiHOG000238220.
    InParanoidiQ9FHX5.
    OMAiILDYHAR.
    PhylomeDBiQ9FHX5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9FHX5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASSSLQSLF SLFCLALFSL PLIVSSIGIN YGQVANNLPP PKNVIPLLKS    50
    VGATKVKLYD ADPQALRAFA GSGFELTVAL GNEYLAQMSD PIKAQGWVKE 100
    NVQAYLPNTK IVAIVVGNEV LTSNQSALTA ALFPAMQSIH GALVDCGLNK 150
    QIFVTTAHSL AILDVSYPPS ATSFRRDLLG SLTPILDFHV KTGSPILINA 200
    YPFFAYEENP KHVSLDFVLF QPNQGFTDPG SNFHYDNMLF AQVDAVYHAL 250
    DAVGISYKKV PIVVSETGWP SNGDPQEVGA TCDNARKYNG NLIKMMMSKK 300
    MRTPIRPECD LTIFVFALFN ENMKPGPTSE RNYGLFNPDG TPVYSLGIKT 350
    SSTHSSGSGS SNSTGGSSSG GGGNTGGSSS GGGIYQPVTG NPSPDYMSIS 400
    SAGGKGRFVE CVLFFFLLCI IKLRL 425
    Length:425
    Mass (Da):45,358
    Last modified:March 1, 2001 - v1
    Checksum:i972C1FC722408DA8
    GO
    Isoform 2 (identifier: Q9FHX5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         404-423: GKGRFVECVLFFFLLCIIKL → VTFSTLHFICCFTLTIFVDI

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:425
    Mass (Da):45,333
    Checksum:i12CEA28040320871
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei404 – 42320GKGRF…CIIKL → VTFSTLHFICCFTLTIFVDI in isoform 2. CuratedVSP_020752Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017067 Genomic DNA. Translation: BAB08443.1.
    CP002688 Genomic DNA. Translation: AED94767.1.
    BT008863 mRNA. Translation: AAP68302.1.
    AY054690 mRNA. Translation: AAK96881.1.
    AY084866 mRNA. Translation: AAM61429.1.
    RefSeqiNP_199025.1. NM_123575.3. [Q9FHX5-1]
    UniGeneiAt.67156.
    At.7792.

    Genome annotation databases

    EnsemblPlantsiAT5G42100.1; AT5G42100.1; AT5G42100. [Q9FHX5-1]
    GeneIDi834215.
    KEGGiath:AT5G42100.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017067 Genomic DNA. Translation: BAB08443.1 .
    CP002688 Genomic DNA. Translation: AED94767.1 .
    BT008863 mRNA. Translation: AAP68302.1 .
    AY054690 mRNA. Translation: AAK96881.1 .
    AY084866 mRNA. Translation: AAM61429.1 .
    RefSeqi NP_199025.1. NM_123575.3. [Q9FHX5-1 ]
    UniGenei At.67156.
    At.7792.

    3D structure databases

    ProteinModelPortali Q9FHX5.
    SMRi Q9FHX5. Positions 27-346.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi Q9FHX5.
    PRIDEi Q9FHX5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G42100.1 ; AT5G42100.1 ; AT5G42100 . [Q9FHX5-1 ]
    GeneIDi 834215.
    KEGGi ath:AT5G42100.

    Organism-specific databases

    TAIRi AT5G42100.

    Phylogenomic databases

    eggNOGi NOG328711.
    HOGENOMi HOG000238220.
    InParanoidi Q9FHX5.
    OMAi ILDYHAR.
    PhylomeDBi Q9FHX5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G42100-MONOMER.
    ARA:GQT-196-MONOMER.

    Gene expression databases

    Genevestigatori Q9FHX5.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
      DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
      Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
      Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
      Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
      J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiE1310_ARATH
    AccessioniPrimary (citable) accession number: Q9FHX5
    Secondary accession number(s): Q3E8I9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3