Q9FHX5 (E1310_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase 10
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase 10
Short name=(1->3)-beta-glucanase 10
Beta-1,3-endoglucanase 10
Short name=Beta-1,3-glucanase 10

Gene names

Ordered Locus Names:	At5g42100
ORF Names:	MJC20.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › cell wall Potential. Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Plant defense
Cellular component	Cell membrane Cell wall Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell communication Inferred from mutant phenotype PubMed 17270015. Source: TAIR defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to plasma membrane Inferred from direct assay Ref.5. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 17270015. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plant-type cell wall Inferred from direct assay PubMed 16287169. Source: TAIR plasmodesma Inferred from direct assay PubMed 17270015PubMed 21533090. Source: TAIR
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from mutant phenotype PubMed 17270015. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FHX5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FHX5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     404-423: GKGRFVECVLFFFLLCIIKL → VTFSTLHFICCFTLTIFVDI

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 401	375	Glucan endo-1,3-beta-glucosidase 10		PRO_0000251267
	Propeptide	402 – 425	24	Removed in mature form Potential		PRO_0000251268
Regions
	Compositional bias	370 – 373	4	Poly-Gly
Sites
	Active site	266	1	Nucleophile By similarity
	Active site	330	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	401	1	GPI-anchor amidated serine Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	404 – 423	20	GKGRF…CIIKL → VTFSTLHFICCFTLTIFVDI in isoform 2.		VSP_020752

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 972C1FC722408DA8	FASTA	425	45,358
	10 20 30 40 50 60 MASSSLQSLF SLFCLALFSL PLIVSSIGIN YGQVANNLPP PKNVIPLLKS VGATKVKLYD 70 80 90 100 110 120 ADPQALRAFA GSGFELTVAL GNEYLAQMSD PIKAQGWVKE NVQAYLPNTK IVAIVVGNEV 130 140 150 160 170 180 LTSNQSALTA ALFPAMQSIH GALVDCGLNK QIFVTTAHSL AILDVSYPPS ATSFRRDLLG 190 200 210 220 230 240 SLTPILDFHV KTGSPILINA YPFFAYEENP KHVSLDFVLF QPNQGFTDPG SNFHYDNMLF 250 260 270 280 290 300 AQVDAVYHAL DAVGISYKKV PIVVSETGWP SNGDPQEVGA TCDNARKYNG NLIKMMMSKK 310 320 330 340 350 360 MRTPIRPECD LTIFVFALFN ENMKPGPTSE RNYGLFNPDG TPVYSLGIKT SSTHSSGSGS 370 380 390 400 410 420 SNSTGGSSSG GGGNTGGSSS GGGIYQPVTG NPSPDYMSIS SAGGKGRFVE CVLFFFLLCI IKLRL
	Isoform 2 [UniParc]. Checksum: 12CEA28040320871	FASTA	425	45,333
	10 20 30 40 50 60 MASSSLQSLF SLFCLALFSL PLIVSSIGIN YGQVANNLPP PKNVIPLLKS VGATKVKLYD 70 80 90 100 110 120 ADPQALRAFA GSGFELTVAL GNEYLAQMSD PIKAQGWVKE NVQAYLPNTK IVAIVVGNEV 130 140 150 160 170 180 LTSNQSALTA ALFPAMQSIH GALVDCGLNK QIFVTTAHSL AILDVSYPPS ATSFRRDLLG 190 200 210 220 230 240 SLTPILDFHV KTGSPILINA YPFFAYEENP KHVSLDFVLF QPNQGFTDPG SNFHYDNMLF 250 260 270 280 290 300 AQVDAVYHAL DAVGISYKKV PIVVSETGWP SNGDPQEVGA TCDNARKYNG NLIKMMMSKK 310 320 330 340 350 360 MRTPIRPECD LTIFVFALFN ENMKPGPTSE RNYGLFNPDG TPVYSLGIKT SSTHSSGSGS 370 380 390 400 410 420 SNSTGGSSSG GGGNTGGSSS GGGIYQPVTG NPSPDYMSIS SAGVTFSTLH FICCFTLTIF VDIRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017067 Genomic DNA. Translation: BAB08443.1. CP002688 Genomic DNA. Translation: AED94767.1. BT008863 mRNA. Translation: AAP68302.1. AY054690 mRNA. Translation: AAK96881.1. AY084866 mRNA. Translation: AAM61429.1.
IPI	IPI00523319. IPI00526574.
RefSeq	NP_199025.1. NM_123575.3.
UniGene	At.67156. At.7792.
3D structure databases
ProteinModelPortal	Q9FHX5.
SMR	Q9FHX5. Positions 27-346.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PaxDb	Q9FHX5.
PRIDE	Q9FHX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G42100.1; AT5G42100.1; AT5G42100.
GeneID	834215.
KEGG	ath:AT5G42100.
Organism-specific databases
TAIR	At5g42100.
Phylogenomic databases
eggNOG	NOG328711.
HOGENOM	HOG000238220.
InParanoid	Q9FHX5.
OMA	PSSAYFR.
PhylomeDB	Q9FHX5.
ProtClustDB	CLSN2680350.
Gene expression databases
ArrayExpress	Q9FHX5.
Genevestigator	Q9FHX5.
GermOnline	AT5G42100. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1310_ARATH

Accession

Primary (citable) accession number: Q9FHX5
Secondary accession number(s): Q3E8I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program