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Q9FHX5

- E1310_ARATH

UniProt

Q9FHX5 - E1310_ARATH

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Protein
Glucan endo-1,3-beta-glucosidase 10
Gene
At5g42100, MJC20.21
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei266 – 2661Nucleophile By similarity
Active sitei330 – 3301Nucleophile By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell communication Source: TAIR
  3. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT5G42100-MONOMER.
ARA:GQT-196-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 10 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 10
Short name:
(1->3)-beta-glucanase 10
Beta-1,3-endoglucanase 10
Short name:
Beta-1,3-glucanase 10
Gene namesi
Ordered Locus Names:At5g42100
ORF Names:MJC20.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G42100.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: TAIR
  2. anchored component of plasma membrane Source: TAIR
  3. cell wall Source: TAIR
  4. endoplasmic reticulum Source: TAIR
  5. extracellular region Source: UniProtKB-KW
  6. plant-type cell wall Source: TAIR
  7. plasma membrane Source: TAIR
  8. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Chaini27 – 401375Glucan endo-1,3-beta-glucosidase 10
PRO_0000251267Add
BLAST
Propeptidei402 – 42524Removed in mature form Reviewed prediction
PRO_0000251268Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi362 – 3621N-linked (GlcNAc...) Reviewed prediction
Lipidationi401 – 4011GPI-anchor amidated serine Reviewed prediction

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9FHX5.
PRIDEiQ9FHX5.

Expressioni

Gene expression databases

GenevestigatoriQ9FHX5.

Structurei

3D structure databases

ProteinModelPortaliQ9FHX5.
SMRiQ9FHX5. Positions 27-346.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi370 – 3734Poly-Gly

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG328711.
HOGENOMiHOG000238220.
InParanoidiQ9FHX5.
OMAiILDYHAR.
PhylomeDBiQ9FHX5.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FHX5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASSSLQSLF SLFCLALFSL PLIVSSIGIN YGQVANNLPP PKNVIPLLKS    50
VGATKVKLYD ADPQALRAFA GSGFELTVAL GNEYLAQMSD PIKAQGWVKE 100
NVQAYLPNTK IVAIVVGNEV LTSNQSALTA ALFPAMQSIH GALVDCGLNK 150
QIFVTTAHSL AILDVSYPPS ATSFRRDLLG SLTPILDFHV KTGSPILINA 200
YPFFAYEENP KHVSLDFVLF QPNQGFTDPG SNFHYDNMLF AQVDAVYHAL 250
DAVGISYKKV PIVVSETGWP SNGDPQEVGA TCDNARKYNG NLIKMMMSKK 300
MRTPIRPECD LTIFVFALFN ENMKPGPTSE RNYGLFNPDG TPVYSLGIKT 350
SSTHSSGSGS SNSTGGSSSG GGGNTGGSSS GGGIYQPVTG NPSPDYMSIS 400
SAGGKGRFVE CVLFFFLLCI IKLRL 425
Length:425
Mass (Da):45,358
Last modified:March 1, 2001 - v1
Checksum:i972C1FC722408DA8
GO
Isoform 2 (identifier: Q9FHX5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-423: GKGRFVECVLFFFLLCIIKL → VTFSTLHFICCFTLTIFVDI

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:425
Mass (Da):45,333
Checksum:i12CEA28040320871
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei404 – 42320GKGRF…CIIKL → VTFSTLHFICCFTLTIFVDI in isoform 2.
VSP_020752Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017067 Genomic DNA. Translation: BAB08443.1.
CP002688 Genomic DNA. Translation: AED94767.1.
BT008863 mRNA. Translation: AAP68302.1.
AY054690 mRNA. Translation: AAK96881.1.
AY084866 mRNA. Translation: AAM61429.1.
RefSeqiNP_199025.1. NM_123575.3. [Q9FHX5-1]
UniGeneiAt.67156.
At.7792.

Genome annotation databases

EnsemblPlantsiAT5G42100.1; AT5G42100.1; AT5G42100. [Q9FHX5-1]
GeneIDi834215.
KEGGiath:AT5G42100.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017067 Genomic DNA. Translation: BAB08443.1 .
CP002688 Genomic DNA. Translation: AED94767.1 .
BT008863 mRNA. Translation: AAP68302.1 .
AY054690 mRNA. Translation: AAK96881.1 .
AY084866 mRNA. Translation: AAM61429.1 .
RefSeqi NP_199025.1. NM_123575.3. [Q9FHX5-1 ]
UniGenei At.67156.
At.7792.

3D structure databases

ProteinModelPortali Q9FHX5.
SMRi Q9FHX5. Positions 27-346.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbi Q9FHX5.
PRIDEi Q9FHX5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G42100.1 ; AT5G42100.1 ; AT5G42100 . [Q9FHX5-1 ]
GeneIDi 834215.
KEGGi ath:AT5G42100.

Organism-specific databases

TAIRi AT5G42100.

Phylogenomic databases

eggNOGi NOG328711.
HOGENOMi HOG000238220.
InParanoidi Q9FHX5.
OMAi ILDYHAR.
PhylomeDBi Q9FHX5.

Enzyme and pathway databases

BioCyci ARA:AT5G42100-MONOMER.
ARA:GQT-196-MONOMER.

Gene expression databases

Genevestigatori Q9FHX5.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE1310_ARATH
AccessioniPrimary (citable) accession number: Q9FHX5
Secondary accession number(s): Q3E8I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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