Reviewed, UniProtKB/Swiss-Prot Q9FHW7 (SKP1B_ARATH)

Names and origin

Protein names

Recommended name:
    SKP1-like protein 1B
Alternative name(s):
    SKP1-like 2
    UFO-binding protein 2

Gene names

Name:	SKP1B
Synonyms:	ASK2, UIP2
Ordered Locus Names:	At5g42190
ORF Names:	MJC20.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF E3 ubiquitin ligase complex composed of SKP1, CUL1, RBX1 (RBX1A or RBX1B) and F-box proteins such as UFO, EID1, ADO1/ZTL, ADO2/LKP2, ADO3/FKF1, COI1 and TIR1. The SKP1B subunit of the SCF E3 ubiquitin ligase complex can probably interact directly with KIN10, KIN11 and the proteasome subunit PAD1. May also interact with SKIP1, SKIP2, SKIP3, SKIP4, SKIP6 and FIB1/SKIP7, AFR, ORE9, EBF1 and EBF2.
Subcellular location	NucleusBy similarity.
Tissue specificity	Expressed in tips, cortical layer and epidermis of roots. Detected in whole seedling, vascular tissues, pith and vascular bundle of young stem, leaves, inflorescence meristem, young floral buds and organ primordia, flowers, developing seeds and through the valve of siliques. Expressed in male meiocytes, pollen, embryo and endosperm.
Developmental stage	Expressed during all stages of embryogenesis.
Sequence similarities	Belongs to the SKP1 family.

Ontologies

Keywords
Biological process	Auxin signaling pathway Chromosome partition Ethylene signaling pathway Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Ref.9 Ref.11 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q0WRC9	2	EBI-604076,EBI-604757
	Q65967	3	EBI-604076,EBI-848577	From a different organism.
	Q9LU91	2	EBI-604076,EBI-604313
ADO1	Q94BT6	2	EBI-604076,EBI-300691
ADO3	Q9C9W9	2	EBI-604076,EBI-401228
At2g01620/T8O11.21	Q9ZU90	2	EBI-604076,EBI-604427
COI1	O04197	5	EBI-604076,EBI-401159
EBF1	Q9SKK0	3	EBI-604076,EBI-401198
EID1	Q8LEA8	2	EBI-604076,EBI-687388
FBL20	Q9M0U9	2	EBI-604076,EBI-604728
FBP7	Q9XI00	2	EBI-604076,EBI-591127
FBW2	Q9ZPE4	2	EBI-604076,EBI-604740
FBX3	O49279	3	EBI-604076,EBI-591174
GID2	Q9STX3	1	EBI-604076,EBI-619033
PP2A13	Q9LEX0	3	EBI-604076,EBI-604261
PP2A14	Q9FJ80	2	EBI-604076,EBI-604303
SKIP1	Q9FDX1	2	EBI-604076,EBI-604228
T21E18.16	Q9LND7	2	EBI-604076,EBI-591078
TIR1	Q570C0	4	EBI-604076,EBI-307183
UFO	Q39090	2	EBI-604076,EBI-590758
virF	P15597	4	EBI-604076,EBI-605118	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 171	171	SKP1-like protein 1B

Sequences

	Sequence		Length	Mass (Da)
	Q9FHW7-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9695CC9F57FC935F	FASTA	171	19,097
	10 20 30 40 50 60 MSTVRKITLK SSDGENFEID EAVALESQTI KHMIEDDCTD NGIPLPNVTS KILSKVIEYC 70 80 90 100 110 120 KRHVEAAEKS ETTADAAAAT TTTTVASGSS DEDLKTWDSE FIKVDQGTLF DLILAANYLN 130 140 150 160 170 IKGLLDLTCQ TVADMIKGKT PEEIRKTFNI KNDFTPEEEE EVRRENQWAF E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an F-box protein required for normal patterning and growth in the floral meristem." Samach A., Klenz J.E., Kohalmi S.E., Risseeuw E., Haughn G.W., Crosby W.L. Plant J. 20:433-445(1999) [PubMed: 10607296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH UFO. Strain: cv. Columbia.
[2]	"Overexpression of Arabidopsis thaliana SKP1 homologues in yeast inactivates the Mig1 repressor by destabilising the F-box protein Grr1." Schouten J., de Kam R.J., Fetter K., Hoge J.H.C. Mol. Gen. Genet. 263:309-319(2000) [PubMed: 10778750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana." Gray W.M., del Pozo J.C., Walker L., Hobbie L., Risseeuw E., Banks T., Crosby W.L., Yang M., Ma H., Estelle M. Genes Dev. 13:1678-1691(1999) [PubMed: 10398681] [Abstract] Cited for: FUNCTION, INTERACTION WITH TIR1.
[6]	"EID1, an F-box protein involved in phytochrome A-specific light signaling." Dieterle M., Zhou Y.-C., Schaefer E., Funk M., Kretsch T. Genes Dev. 15:939-944(2001) [PubMed: 11316788] [Abstract] Cited for: INTERACTION WITH EID1.
[7]	"The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects." Lechner E., Xie D., Grava S., Pigaglio E., Planchais S., Murray J.A.H., Parmentier Y., Mutterer J., Dubreucq B., Shen W.-H., Genschik P. J. Biol. Chem. 277:50069-50080(2002) [PubMed: 12381738] [Abstract] Cited for: IDENTIFICATION IN A SCF COMPLEX.
[8]	"The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis." Xu L., Liu F., Lechner E., Genschik P., Crosby W.L., Ma H., Peng W., Huang D., Xie D. Plant Cell 14:1919-1935(2002) [PubMed: 12172031] [Abstract] Cited for: INTERACTION WITH COI1, IDENTIFICATION IN A SCF(COI1) COMPLEX.
[9]	"COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis." Devoto A., Nieto-Rostro M., Xie D., Ellis C., Harmston R., Patrick E., Davis J., Sherratt L., Coleman M., Turner J.G. Plant J. 32:457-466(2002) [PubMed: 12445118] [Abstract] Cited for: INTERACTION WITH COI1, IDENTIFICATION IN A SCF(COI1) COMPLEX.
[10]	"Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis." Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H. Plant Physiol. 133:203-217(2003) [PubMed: 12970487] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[11]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH ADO1; ADO2 AND ADO3.
[12]	"The ASK1 and ASK2 genes are essential for Arabidopsis early development." Liu F., Ni W., Griffith M.E., Huang Z., Chang C., Peng W., Ma H., Xie D. Plant Cell 16:5-20(2004) [PubMed: 14688296] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[13]	"Formation of an SCF(ZTL) complex is required for proper regulation of circadian timing." Han L., Mason M., Risseeuw E.P., Crosby W.L., Somers D.E. Plant J. 40:291-301(2004) [PubMed: 15447654] [Abstract] Cited for: INTERACTION WITH ADO1.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	U97021 mRNA. Translation: AAC63110.1. Different initiation. AF059295 mRNA. Translation: AAC14445.1. AB017067 Genomic DNA. Translation: BAB08452.1. BT004798 mRNA. Translation: AAO44064.1.
RefSeq	NP_568603.1.
UniGene	At.71056
3D structure databases
HSSP	HSSP built from PDB template 1FS1 based on UniProtKB P34991.
SMR	Q9FHW7. Positions 6-171.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FHW7.
Genome annotation databases
GeneID	834224.
GenomeReviews	Gene locus AT5G42190 in contig BA000015_GR.
KEGG	ath:AT5G42190.
NMPDR	fig|3702.1.peg.25940.
Organism-specific databases
GeneFarm	4982.
TAIR	At5g42190.
Gene expression databases
GermOnline	AT5G42190. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011333. BTB/POZ_fold. IPR016897. E3_ubiquit_lig_SCF_Skp. IPR001232. Skp1_comp. IPR016072. Skp1_comp_dimer. IPR016073. Skp1_comp_POZ. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
PANTHER	PTHR11165. Skp1. 1 hit.
Pfam	PF01466. Skp1. 1 hit. PF03931. Skp1_POZ. 1 hit. [Graphical view]
PIRSF	PIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMART	SM00512. Skp1. 1 hit. [Graphical view]
ProDom	Q9FHW7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SKP1B_ARATH

Accession

Primary (citable) accession number: Q9FHW7
Secondary accession number(s): O65283

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2001
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)