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Reviewed, UniProtKB/Swiss-Prot Q9FHN4 (PME60_ARATH)

Last modified October 13, 2009. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable pectinesterase/pectinesterase inhibitor 60
Including the following 2 domains:
    1- Recommended name:
            Pectinesterase inhibitor 60
        Alternative name(s):
            Pectin methylesterase inhibitor 60
    2- Recommended name:
            Pectinesterase 60
                Short name=PE 60
              EC=3.1.1.11
        Alternative name(s):
            Pectin methylesterase 60
              Short name=AtPME60
Gene names
Name: PME60
Synonyms: ARATH60
Ordered Locus Names: At5g51500
ORF Names: K17N15.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length540 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.

Catalytic activity

Pectin + n H2O = n methanol + pectate.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subcellular location

Secreted › cell wall By similarity.

Tissue specificity

Expressed in siliques. Ref.3

Developmental stage

Expressed during late developmental phases of siliques. Ref.3

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Sequence similarities

In the N-terminal section; belongs to the PMEI family.

In the C-terminal section; belongs to the pectinesterase family.

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Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell wall
Secreted
   DomainSignal
   Molecular functionAspartyl esterase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcell wall modification

Inferred from electronic annotation. Source: InterPro

   Cellular componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionaspartyl esterase activity

Inferred from electronic annotation. Source: UniProtKB-KW

enzyme inhibitor activity

Inferred from electronic annotation. Source: InterPro

pectinesterase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 540509Probable pectinesterase/pectinesterase inhibitor 60
PRO_0000370179

Regions

Region32 – 185154Pectinesterase inhibitor 60
Region225 – 526302Pectinesterase 60

Sites

Active site3551Proton donor; for pectinesterase activity By similarity
Active site3761Nucleophile; for pectinesterase activity By similarity
Binding site3021Substrate; for pectinesterase activity By similarity
Binding site3321Substrate; for pectinesterase activity By similarity
Binding site4441Substrate; for pectinesterase activity By similarity
Binding site4461Substrate; for pectinesterase activity By similarity
Site3541Transition state stabilizer By similarity

Amino acid modifications

Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1201N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1951N-linked (GlcNAc...) Potential
Disulfide bond369 ↔ 389 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9FHN4-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: B33E471B758CA5D8

FASTA54059,648
        10         20         30         40         50         60 
MNIMMVQNIS FLSLHLLLIL LLCLRPLTTV ADGNSTNIDG WCDKTPYPYP CKRYFIKHSG 

        70         80         90        100        110        120 
FRLPTQISEF RVLLVEAAMD RAVSAWDKLT NSSKNCTDFK KQAVLADCIN LYGDTVMQLN 

       130        140        150        160        170        180 
RTLQGVSSKT GRRCTDFDAQ TWLSTALTNT ETCRRGSSDL NVSDFTTPIV SNTKISHLIS 

       190        200        210        220        230        240 
NCLAVNGALL TAGKNDSTTG DSKGFPTWVS RKERRLLQLQ SVRANLVVAK DGSGHFKTVQ 

       250        260        270        280        290        300 
AAIDVAGRRK VTSGRFVIYV KRGIYQENLN VRLNNDNIML VGDGMRYTII TGGRSVKGGY 

       310        320        330        340        350        360 
TTYSSATAGI EGLHFIAKGI AFQNTAGPAK GQAVALRSSS DLSIFYRCSI EGYQDTLMVH 

       370        380        390        400        410        420 
SQRQFYRECY IYGTVDFIFG NAAVVFQNCI ILPRLPLKGQ ANVITAQGRT DLFQNTGISI 

       430        440        450        460        470        480 
HNSIIIPAPD LKPVVRSVKT YMGRPWMMYS RTVVLKTYID SVVSPVGWSP WTKGSTYGLD 

       490        500        510        520        530        540 
TLFYAEYKNI GPASSTRWRV RWKGFHVLSK ASDASAFSVG KFIAGTAWLP GSGIPFTSEL

« Hide

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References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Pectin methylesterases: sequence-structural features and phylogenetic relationships."
Markovic O., Janecek S.
Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[3]"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana."
Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J.
Planta 224:782-791(2006) [PubMed: 16622707] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

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Cross-references

Sequence databases

AB018109 Genomic DNA. Translation: BAB08666.1.
IPIIPI00546622.
RefSeqNP_199963.1.
UniGeneAt.29676

3D structure databases

HSSPHSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ModBaseSearch...

Genome annotation databases

GeneID835224.
GenomeReviewsGene locus AT5G51500 in contig BA000015_GR.
KEGGath:AT5G51500.
NMPDRfig|3702.1.peg.27020.

Organism-specific databases

GeneFarm304. 8.
TAIRAt5g51500.

Gene expression databases

GenevestigatorQ9FHN4.

Family and domain databases

InterProIPR012334. Pectin_lyas_fold.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
PfamPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
TIGRFAMsTIGR01614. PME_inhib. 1 hit.
PROSITEPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePME60_ARATH
AccessionPrimary (citable) accession number: Q9FHN4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: October 13, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents