65-kDa microtubule-associated protein 3 - Arabidopsis thaliana (Mouse-ear cress)

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Q9FHM4 (MA653_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 58. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
65-kDa microtubule-associated protein 3
Short name=AtMAP65-3

Alternative name(s):
Protein PLEIADE

Gene names

Name:	MAP65-3
Synonyms:	PLE
Ordered Locus Names:	At5g51600
ORF Names:	K17N15.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-associated protein that plays a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. Essential for the cytokinesis, especially in roots, by maintaining the integrity of the overlapped microtubules in the phragmoplast. Required during root morphogenesis. Needed for giant cell development during root knot nematode infection, where cytokinesis is initiated but not completed. Ref.4 Ref.5 Ref.6 Ref.8
Subunit structure	Forms a dimer By similarity. Binds to microtubules (MT) during cell division. Bundles polymerized MT via the formation of 25-nm crossbridges with centrally located endocytic MT, and midline phragmoplast MT. Ref.6 Ref.7
Subcellular location	Nucleus. Cytoplasm. Cytoplasm › cytoskeleton › phragmoplast. Note: Locates only to the mitotic MT arrays. Present in MT cortical arrays just before mitosis. Associates to MT in preprophase band, during anaphase, and in phragmoplast, including midzone. Distributed diffusely through the cytoplasm during metaphase only. At the end of cytokinesis, present only at the cell periphery, forming a ring around the newly formed cell plate. During root knot nematode infection, targeted to the giant cell mini cell plate. Ref.6 Ref.7 Ref.8 Ref.9
Tissue specificity	Expressed in all tissues enriched in dividing cells, such as the root and shoot apical meristem, foliar primordia, and young leaves, and embryos. Ref.8
Developmental stage	Present in dividing tissues during all stages of embryonic development. Ref.8
Disruption phenotype	Irregular root expansion. Defects in karyokinesis and cytokinesis. Cytokinesis defects before the embryo dermatogen stage and in roots. Embryos with enlarged nuclei, often containing multiple nucleoli. Some abnormal stomata with pores attached to a single side of the mother cell. Long root hairs. In roots, multinucleated cells, cell wall stubs, and synchronized cell divisions in incompletely separated cells. Normal anaphase spindle, but distorted phragmoplast. Abnormal formation of nematode-mediated giant cells leading to impaired maturation of nematode larvae. Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the MAP65/ASE1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytokinesis by cell plate formation Inferred from mutant phenotype Ref.5 Ref.8. Source: TAIR formation by symbiont of syncytium involving giant cell for nutrient acquisition from host Inferred from mutant phenotype Ref.8. Source: TAIR microtubule polymerization Inferred from direct assay Ref.9. Source: TAIR mitosis Inferred from electronic annotation. Source: UniProtKB-KW nuclear division Inferred from mutant phenotype Ref.8. Source: TAIR response to nematode Inferred from mutant phenotype Ref.8. Source: UniProtKB
Cellular_component	cortical microtubule Inferred from direct assay Ref.9. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell phragmoplast Inferred from direct assay Ref.7 Ref.8 Ref.9. Source: TAIR preprophase band Inferred from direct assay Ref.9. Source: TAIR
Molecular_function	microtubule binding Inferred from direct assay Ref.9. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 707

707

65-kDa microtubule-associated protein 3

PRO_0000395474

Regions

Coiled coil

49 – 84

Potential

Coiled coil

157 – 179

Potential

Coiled coil

269 – 289

Potential

Coiled coil

354 – 374

Potential

Coiled coil

464 – 486

Potential

Sites

Site

410

Microtubule binding By similarity

Site

421

Microtubule binding

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

504

Phosphoserine By similarity

Modified residue

528

Phosphoserine By similarity

Experimental info

Mutagenesis

421

A → V in ple-4; impaired microtubule binding. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q9FHM4 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 30EAF1E5BCC6BB9A
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FASTA

707

80,302

        10         20         30         40         50         60 
MASVQKDPIL QVETTCGSLL FELQIIWDEV GETETDRDQM LLELERECLE VYRRKVDQAN 

        70         80         90        100        110        120 
RCRAQLRQAI ADAEAQLAAI CSAMGERPVH IRQSDQSVGS LKQELGRILP ELEEMQKRKV 

       130        140        150        160        170        180 
ERRNQFIVVM EQIDSITNDI KGQGELVHSE PLIDETNLSM RKLEELHCQL QVLQKEKIDR 

       190        200        210        220        230        240 
VETIRKHLCT LYSHCSVLGM DFNEVVGQVN PTLSDPEGPR SLSDHTIEKL GAAVQKLMEV 

       250        260        270        280        290        300 
KIQRMQRLQD LATTMLELWN LMDTPIEEQQ EYQHITCNIA ASEHEITEAN SLSEDFIKYV 

       310        320        330        340        350        360 
EAEVVRLDEV KASKMKELVL KKRSELEEIC RKTHLLPVSD SAIDQTIVAI ESGIVDATMV 

       370        380        390        400        410        420 
LEHLEQHISK IKEEALSRKE ILERVEKWLS ACDEESWLEE YNRDDNRYNA GRGAHLTLKR 

       430        440        450        460        470        480 
AEKARNLVTK LPGMVEALAS KTIVWEQENG IEFLYDGIRL LSMLEEYNIL RQEREEEHRR 

       490        500        510        520        530        540 
QRDQKKLQGQ LIAEQEALYG SKPSPSKPLG GKKAPRMSTG GASNRRLSLG AAMHQTPKPN 

       550        560        570        580        590        600 
KKADHRHNDG ALSNGRRGLD IAGLPSRKQS MNPSEMLQSP LVRKPFSPIS TTVVASKANI 

       610        620        630        640        650        660 
ATTTTQQLPK NNAVNEISSF ATPIKNNNIL RNLEEEKMMT MMMQTPKNVA AMIPIPSTPA 

       670        680        690        700 
TVSVPMHTAP TPFTNNARLM SEKPEVVEYS FEERRLAFML QSECRLV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"The plant cytoskeleton: recent advances in the study of the plant microtubule-associated proteins MAP-65, MAP-190 and the Xenopus MAP215-like protein, MOR1." Hussey P.J., Hawkins T.J., Igarashi H., Kaloriti D., Smertenko A. Plant Mol. Biol. 50:915-924(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Cytokinesis-defective mutants of Arabidopsis." Soellner R., Glaesser G., Wanner G., Somerville C.R., Juergens G., Assaad F.F. Plant Physiol. 129:678-690(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]	"Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis." Mueller S., Fuchs E., Ovecka M., Wysocka-Diller J., Benfey P.N., Hauser M.-T. Plant Physiol. 130:312-324(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ALA-421, DISRUPTION PHENOTYPE.
[6]	"The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function." Mueller S., Smertenko A., Wagner V., Heinrich M., Hussey P.J., Hauser M.-T. Curr. Biol. 14:412-417(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION WITH MICROTUBULES.
[7]	"Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins." Van Damme D., Bouget F.-Y., Van Poucke K., Inze D., Geelen D. Plant J. 40:386-398(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[8]	"MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis." Caillaud M.-C., Lecomte P., Jammes F., Quentin M., Pagnotta S., Andrio E., de Almeida Engler J., Marfaing N., Gounon P., Abad P., Favery B. Plant Cell 20:423-437(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[9]	"The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes." Smertenko A.P., Kaloriti D., Chang H.-Y., Fiserova J., Opatrny Z., Hussey P.J. Plant Cell 20:3346-3358(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018109 Genomic DNA. Translation: BAB08676.1. CP002688 Genomic DNA. Translation: AED96102.1.
IPI	IPI00537287.
RefSeq	NP_199973.1. NM_124539.3.
UniGene	At.29670.
3D structure databases
ProteinModelPortal	Q9FHM4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FHM4. 1 interaction.
STRING	3702.AT5G51600.1-P.
Proteomic databases
PaxDb	Q9FHM4.
PRIDE	Q9FHM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G51600.1; AT5G51600.1; AT5G51600.
GeneID	835234.
KEGG	ath:AT5G51600.
Organism-specific databases
TAIR	At5g51600.
Phylogenomic databases
eggNOG	NOG298643.
HOGENOM	HOG000238749.
InParanoid	Q9FHM4.
KO	K16732.
OMA	HYDIDSA.
PhylomeDB	Q9FHM4.
ProtClustDB	CLSN2687413.
Gene expression databases
Genevestigator	Q9FHM4.
Family and domain databases
InterPro	IPR007145. MAP65_Ase1_PRC1. [Graphical view]
PANTHER	PTHR19321. PTHR19321. 1 hit.
Pfam	PF03999. MAP65_ASE1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MA653_ARATH

Accession

Primary (citable) accession number: Q9FHM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents