Q9FHE1 (GSTT3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase T3
Short name=AtGSTT3
EC=2.5.1.18

Alternative name(s):
GST class-theta member 3
Glutathione S-transferase 10C

Gene names

Name:	GSTT3
Synonyms:	GST10C
Ordered Locus Names:	At5g41220
ORF Names:	K1O13.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Nucleus Ref.4.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain. Contains 1 Myb-like domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Nucleus
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxic substance Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.5. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.5. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Glutathione S-transferase T3		PRO_0000413576
Regions
	Domain	1 – 82	82	GST N-terminal
	Domain	89 – 232	144	GST C-terminal
	Domain	265 – 336	72	Myb-like
	Region	11 – 12	2	Glutathione binding By similarity
	Region	40 – 41	2	Glutathione binding By similarity
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FHE1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E85F66525F6C7AF5	FASTA	590	67,555
	10 20 30 40 50 60 MKLKVYADRM SQPSRAVLIF CKVNEIQFDE ILIYLANRQQ LSPEFKDINP MGKVPAIVDG 70 80 90 100 110 120 KLKLSESHAI LIYLSSAYPS VVDHWYPTDL SKRARIHSVL DWHHTNLRPG AAGYVLNSVL 130 140 150 160 170 180 GPALGLPLNP KAAAEAEQLL TKSLTTLDTF WLKGNAMFLL GSNQPSIADL SLVCELTQLQ 190 200 210 220 230 240 VLDDKDRLRL LSPHKNVEQW IENTRKATMP HFDEVHEVLF RAKDRCQKQR EMATASKPGP 250 260 270 280 290 300 QSKIIQFSTI GEKSDDPNLV QNTTDRRKHR RKWSRAEDAI LISAWLNTSK DPIVDNEHKA 310 320 330 340 350 360 CAFWKRIGAY FNNSASLANL PKREPSHCKQ RWSKLNDKVC KFVGCYDQAL NQRSSGQSED 370 380 390 400 410 420 DVFQVAYQVY TNNYKSNFTL EHAWRELRHS KKWCSLYPFE NSKGGGSSKR TKLNNGDRVY 430 440 450 460 470 480 SSSSNPESVP IALDEEEQVM DLPLGVKSSK QKEKKVATII TIEEREADSG SRLENLWVLD 490 500 510 520 530 540 EEEQVMDRPL GVKSLEQKEN KVAPKPTIEE REAADSRSRL ENLWALKEKE EREADSRSRL 550 560 570 580 590 ENLWALKEKD IEEQKKLTRM EVLKSLLGRT TDQLSEKEDI LKNKLIDEML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 249-590. Strain: cv. Columbia.
[4]	"Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily." Dixon D.P., Hawkins T., Hussey P.J., Edwards R. J. Exp. Bot. 60:1207-1218(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB019225 Genomic DNA. Translation: BAB11098.1. CP002688 Genomic DNA. Translation: AED94655.1. AK222098 mRNA. Translation: BAD95009.1.
IPI	IPI00541941.
RefSeq	NP_198938.1. NM_123487.3.
UniGene	At.30245.
3D structure databases
ProteinModelPortal	Q9FHE1.
SMR	Q9FHE1. Positions 1-224.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G41220.1-P.
Proteomic databases
PRIDE	Q9FHE1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G41220.1; AT5G41220.1; AT5G41220.
GeneID	834124.
KEGG	ath:AT5G41220.
Organism-specific databases
TAIR	At5g41220.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000148624.
InParanoid	Q9FHE1.
PhylomeDB	Q9FHE1.
ProtClustDB	CLSN2686230.
Gene expression databases
ArrayExpress	Q9FHE1.
Genevestigator	Q9FHE1.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR009057. Homeodomain-like. IPR017877. Myb-like_dom. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. PS50090. MYB_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTT3_ARATH

Accession

Primary (citable) accession number: Q9FHE1
Secondary accession number(s): Q56WE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program