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Protein

Serine/threonine-protein kinase BSK8

Gene

BSK8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable serine/threonine kinase that acts as positive regulator of brassinosteroid (BR) signaling downstream of the receptor kinase BRI1. Functions redundantly with BSK3, BSK4, BSK6 and BSK7 (PubMed:23496207). Involved in the regulation of sucrose-phosphate synthase 1 (SPS1) in the context of sucrose resuply after starvation. Activates BSL2, a phosphatase that may dephosphorylate SPS1, leading to the activation of SPS1 (PubMed:24924143).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71ATPCombined sources1 Publication1
Binding sitei87ATPPROSITE-ProRule annotationCombined sources1 Publication1
Active sitei181Proton acceptorPROSITE-ProRule annotation1
Binding sitei205ATPCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 73ATPPROSITE-ProRule annotation9
Nucleotide bindingi133 – 135ATPCombined sources1 Publication3
Nucleotide bindingi185 – 186ATPCombined sources1 Publication2

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: UniProtKB
  • cell surface receptor signaling pathway Source: GO_Central
  • protein phosphorylation Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processBrassinosteroid signaling pathway
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BSK8Curated (EC:2.7.11.1Curated)
Alternative name(s):
Brassinosteroid-signaling kinase 81 Publication
Gene namesi
Name:BSK81 Publication
Ordered Locus Names:At5g41260Imported
ORF Names:K1O13.5Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT5G41260
TAIRilocus:2155115 AT5G41260

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20S → A: Phosphorylation null-mimic mutant. 1 Publication1
Mutagenesisi20S → D: Constitutive phosphorylation-mimic mutant. 1 Publication1
Mutagenesisi213S → A: Phosphorylation null-mimic mutant. 1 Publication1
Mutagenesisi213S → A: Slightly reduces BSK8 protein phosphorylation. 1 Publication1
Mutagenesisi213S → D: Constitutive phosphorylation-mimic mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003248452 – 487Serine/threonine-protein kinase BSK8Add BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei20PhosphoserineCombined sources1 Publication1
Modified residuei213Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by BRI1, ASK7/BIN2 and ASK9/BIL2.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9FHD7
PRIDEiQ9FHD7

PTM databases

iPTMnetiQ9FHD7
SwissPalmiQ9FHD7

Expressioni

Gene expression databases

ExpressionAtlasiQ9FHD7 differential
GenevisibleiQ9FHD7 AT

Interactioni

Subunit structurei

Interacts with ASK7/BIN2, BSK1, BSK5, BSK6 and BSK11 (PubMed:23496207). Interacts with BSL2 (PubMed:24924143).2 Publications

Protein-protein interaction databases

BioGridi19378, 9 interactors
STRINGi3702.AT5G41260.1

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi46 – 52Combined sources7
Turni53 – 56Combined sources4
Helixi58 – 60Combined sources3
Beta strandi63 – 65Combined sources3
Beta strandi67 – 70Combined sources4
Beta strandi73 – 77Combined sources5
Beta strandi83 – 88Combined sources6
Helixi91 – 93Combined sources3
Helixi97 – 108Combined sources12
Beta strandi118 – 122Combined sources5
Helixi124 – 126Combined sources3
Beta strandi129 – 133Combined sources5
Helixi140 – 145Combined sources6
Beta strandi148 – 150Combined sources3
Helixi154 – 173Combined sources20
Beta strandi186 – 189Combined sources4
Beta strandi195 – 198Combined sources4
Helixi199 – 202Combined sources4
Beta strandi205 – 209Combined sources5
Helixi216 – 218Combined sources3
Helixi221 – 226Combined sources6
Helixi231 – 247Combined sources17
Helixi253 – 261Combined sources9
Helixi265 – 268Combined sources4
Helixi271 – 273Combined sources3
Helixi279 – 292Combined sources14
Helixi297 – 299Combined sources3
Helixi303 – 310Combined sources8
Helixi311 – 313Combined sources3
Helixi321 – 325Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I92X-ray1.60A40-328[»]
4I93X-ray1.50A/B40-328[»]
4I94X-ray1.80A/B40-328[»]
ProteinModelPortaliQ9FHD7
SMRiQ9FHD7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 325Protein kinasePROSITE-ProRule annotationAdd BLAST267

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IGD2 Eukaryota
ENOG410XQTV LUCA
HOGENOMiHOG000239695
InParanoidiQ9FHD7
KOiK14500
OMAiWESQAMK
OrthoDBiEOG093606TW
PhylomeDBiQ9FHD7

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FHD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCEVSKLSA LCCVSESGRS NPDVTGLDEE GRGESNDLPQ FREFSIETIR
60 70 80 90 100
NATSGFAAEN IVSEHGERAP NVVYKGKLEN QRRIAVKRFN RKSWPDSRQF
110 120 130 140 150
LEEAKAVGQL RNHRMANLLG CCYEDEERLL IAEFMPNETL AKHLFHWESQ
160 170 180 190 200
PMKWAMRLRV ALHIAQALEY CTSKGRALYH DLNAYRVLFD DDANPRLSCF
210 220 230 240 250
GLMKNSRDGK SYSTNLAFTP PEYLRTGRVT PESVIYSFGT LLLDLLSGKH
260 270 280 290 300
IPPSHALDLI RDRNIQMLMD SGLEGQFSSD DGTELIRLAS RCLQYEPRER
310 320 330 340 350
PNPKSLVSAM IPLQKDLEIA SHQLLGVPNS ATTTALSPLG EACLRSDLTA
360 370 380 390 400
IHEIIEKLGY KDDEGATTEL SFQMWTDQMQ DTLVFKKKGD SAFRHKDFAK
410 420 430 440 450
AIECYSQFIE VGTMGSPTVH ARQSLCYLMN DMPREALNNA MQAQVISPAW
460 470 480
HIASYLQAVA LSALGQENEA HTALKDGAML ESKRNPL
Length:487
Mass (Da):54,625
Last modified:March 1, 2001 - v1
Checksum:i7619F9C5B8ED5334
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286I → V in BAC42231 (PubMed:11910074).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019225 Genomic DNA Translation: BAB11102.1
CP002688 Genomic DNA Translation: AED94658.1
BT029016 mRNA Translation: ABI93925.1
AK117571 mRNA Translation: BAC42231.1
RefSeqiNP_198942.1, NM_123491.4
UniGeneiAt.43892

Genome annotation databases

EnsemblPlantsiAT5G41260.1; AT5G41260.1; AT5G41260
GeneIDi834127
GrameneiAT5G41260.1; AT5G41260.1; AT5G41260
KEGGiath:AT5G41260

Entry informationi

Entry nameiBSK8_ARATH
AccessioniPrimary (citable) accession number: Q9FHD7
Secondary accession number(s): Q8GYJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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