Q9FH90 (IX14H_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,4-xylosyltransferase IRX14H
EC=2.4.2.-

Alternative name(s):
Protein IRREGULAR XYLEM 14 homolog
Xylan xylosyltransferase IRX14H

Gene names

Name:	IRX14H
Ordered Locus Names:	At5g67230
ORF Names:	K21H1.19

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the elongation of glucuronoxylan xylosyl backbone. Ref.4 Ref.5
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable Ref.4.
Tissue specificity	Expressed in developing interfascicular fibers and xylem cells in stems and developing secondary xylem in roots. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glucuronoxylan biosynthetic process Inferred from mutant phenotype Ref.4. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: InterPro xylosyltransferase activity Inferred from mutant phenotype Ref.4. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Probable beta-1,4-xylosyltransferase IRX14H		PRO_0000407566
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 54	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	55 – 492	438	Lumenal Potential
Amino acid modifications
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	314	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FH90 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BF967F6485610232	FASTA	492	55,349
	10 20 30 40 50 60 MKLSVFRLSY WNRRGSSFRS SPSLDPSFDG KSPSSVFWFV IHGLCCLISL ILGFRFSHLV 70 80 90 100 110 120 LFFLFSTSVT NLYTTPFLFA GNGGVSQLLR LKPLETATNS TVKKNSRVVV GRHGIRIRPW 130 140 150 160 170 180 PHPNPIEVLR AHQLLVRVQK EQKSMYGVRS PRTVIVVTPT YVRTFQALHL TGVMHSLMLV 190 200 210 220 230 240 PYDLVWIVVE AGGITNETAS FIAKSGLKTI HLGFDQKMPN TWEDRHKLET KMRLHALRVV 250 260 270 280 290 300 REKKLDGIVM FADDSNMHSM ELFDEIQTVK WFGALSVGIL AHSGNADELS SILKNEQGKN 310 320 330 340 350 360 KEKPSMPIQG PSCNSSEKLV GWHIFNTQPY AKKTAVYIDE KAPVMPSKME WSGFVLNSRL 370 380 390 400 410 420 LWKESLDDKP AWVKDLSLLD DGYAEIESPL SLVKDPSMVE PLGSCGRRVL LWWLRVEARA 430 440 450 460 470 480 DSKFPPGWII KSPLEITVPS KRTPWPDSSS ELPAAAIKEA KSNSKPRVSK SKSYKEKQEP 490 KAFDGVKVSA TS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone." Lee C., Teng Q., Huang W., Zhong R., Ye Z.H. Plant Physiol. 153:526-541(2010) [PubMed: 20335400] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of glycosyltransferase genes reveals critical contributions to biosynthesis of the hemicellulose glucuronoxylan." Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P., Marchant A. Plant Physiol. 153:542-554(2010) [PubMed: 20424005] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020742 Genomic DNA. Translation: BAB10957.1. CP002688 Genomic DNA. Translation: AED98316.1. AY070076 mRNA. Translation: AAL49771.1.
IPI	IPI00539729.
RefSeq	NP_201524.1. NM_126123.4.
UniGene	At.28408. At.71151.
3D structure databases
HSSP	HSSP built from PDB template 1KWS based on UniProtKB O94766.
ProteinModelPortal	Q9FH90.
SMR	Q9FH90. Positions 153-419.
ModBase	Search...
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Proteomic databases
PRIDE	Q9FH90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G67230.1; AT5G67230.1; AT5G67230.
GeneID	836858.
GenomeReviews	Gene locus AT5G67230 in contig BA000015_GR.
KEGG	ath:AT5G67230.
NMPDR	fig|3702.1.peg.28834.
Organism-specific databases
TAIR	At5g67230.
Phylogenomic databases
eggNOG	KOG1476.
GeneTree	EPGT00070000029587.
HOGENOM	HBG596464.
InParanoid	Q9FH90.
OMA	PLETATN.
PhylomeDB	Q9FH90.
ProtClustDB	CLSN2716060.
Gene expression databases
ArrayExpress	Q9FH90.
Genevestigator	Q9FH90.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IX14H_ARATH

Accession

Primary (citable) accession number: Q9FH90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	March 1, 2001
Last modified:	September 21, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program