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Protein

Beta-amylase 8

Gene

BAM8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei296SubstrateBy similarity1
Binding sitei336SubstrateBy similarity1
Active sitei429Proton donorBy similarity1
Binding sitei537SubstrateBy similarity1
Active sitei623Proton acceptorBy similarity1

GO - Molecular functioni

  • beta-amylase activity Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • polysaccharide catabolic process Source: UniProtKB-KW
  • regulation of shoot system development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amylase 8 (EC:3.2.1.2)
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 2
Gene namesi
Name:BAM8
Synonyms:BMY2
Ordered Locus Names:At5g45300
ORF Names:K9E15.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G45300.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003934221 – 689Beta-amylase 8Add BLAST689

Proteomic databases

PaxDbiQ9FH80.
PRIDEiQ9FH80.

PTM databases

iPTMnetiQ9FH80.

Expressioni

Gene expression databases

GenevisibleiQ9FH80. AT.

Interactioni

Protein-protein interaction databases

BioGridi19815. 1 interactor.
IntActiQ9FH80. 1 interactor.
STRINGi3702.AT5G45300.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FH80.
SMRiQ9FH80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni624 – 625Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 14 family.Curated

Phylogenomic databases

eggNOGiENOG410IMY0. Eukaryota.
ENOG410YA7C. LUCA.
HOGENOMiHOG000238755.
InParanoidiQ9FH80.
OMAiMAFHEYG.
OrthoDBiEOG093604F0.
PhylomeDBiQ9FH80.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR008540. BES1_N.
IPR001554. Glyco_hydro_14.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF05687. BES1_N. 1 hit.
PF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FH80-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTLNNTITT TTGSQDPNLD PIPDPDQFPN RNRNQPQSRR PRGFAAAAAA
60 70 80 90 100
ASIAPTENDV NNGNIAGIGG GEGSSGGGGG GGGKGKRERE KEKERTKLRE
110 120 130 140 150
RHRRAITSRM LAGLRQYGNF PLPARADMND VIAALAREAG WSVEADGTTY
160 170 180 190 200
RQSQQPNHVV QFPTRSIESP LSSSTLKNCA KAAIESQQHS VLRNDEKLAP
210 220 230 240 250
VSLDSIGIAE SDHPGNGRYT SVSPITSVGC LEANQLIQDV HSAEQCNDFT
260 270 280 290 300
ESFYVPVYAM LPVGIIDNFG QLVDPEGVRQ ELSYMKSLNV DGVVIDCWWG
310 320 330 340 350
IVEGWNPQKY VWSGYRELFN LIRDFKLKLQ VVMAFHEYGG NASGNVMISL
360 370 380 390 400
PQWVLKIGKD NPDIFFTDRE GRRSFECLNW SIDKERVLHG RTGIEVYFDF
410 420 430 440 450
MRSFRSEFDD LFVEGLITAV EIGLGASGEL KYPSFPERMG WIYPGIGEFQ
460 470 480 490 500
CYDKYSQLSL QKEAKSRGFT FWGKGPENAG QYSSHPHETV FFQERGEYDS
510 520 530 540 550
YYGRFFLNWY SQLLIGHAEN VLSLANLAFE ETKIIVKIPA IYWSYKTASH
560 570 580 590 600
AAELTAGYYN PSNRDGYSLV FETLKKYSVT VKFVCPGPQM SPNAHEEALA
610 620 630 640 650
DPEGLSWQVI NAAWDKGLQI GGENAITCFD RDGCMRLIDI AKPRNHPDGY
660 670 680
HFSFFTYRQP SPLVQGSTCF PDLDYFIKRM HGDIRDKQF
Length:689
Mass (Da):77,160
Last modified:March 1, 2001 - v1
Checksum:iD591C143B130EC7B
GO
Isoform 2 (identifier: Q9FH80-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-161: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:687
Mass (Da):76,933
Checksum:i2E718FDFDF70EDAC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038980160 – 161Missing in isoform 2. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020744 Genomic DNA. Translation: BAB10251.1.
CP002688 Genomic DNA. Translation: AED95229.1.
CP002688 Genomic DNA. Translation: AED95230.1.
AK117140 mRNA. Translation: BAC41818.1.
BT006482 mRNA. Translation: AAP21290.1.
RefSeqiNP_001032014.1. NM_001036937.1. [Q9FH80-2]
NP_199343.1. NM_123898.3. [Q9FH80-1]
UniGeneiAt.30019.

Genome annotation databases

EnsemblPlantsiAT5G45300.1; AT5G45300.1; AT5G45300. [Q9FH80-1]
GeneIDi834566.
GrameneiAT5G45300.1; AT5G45300.1; AT5G45300.
KEGGiath:AT5G45300.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020744 Genomic DNA. Translation: BAB10251.1.
CP002688 Genomic DNA. Translation: AED95229.1.
CP002688 Genomic DNA. Translation: AED95230.1.
AK117140 mRNA. Translation: BAC41818.1.
BT006482 mRNA. Translation: AAP21290.1.
RefSeqiNP_001032014.1. NM_001036937.1. [Q9FH80-2]
NP_199343.1. NM_123898.3. [Q9FH80-1]
UniGeneiAt.30019.

3D structure databases

ProteinModelPortaliQ9FH80.
SMRiQ9FH80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19815. 1 interactor.
IntActiQ9FH80. 1 interactor.
STRINGi3702.AT5G45300.1.

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

PTM databases

iPTMnetiQ9FH80.

Proteomic databases

PaxDbiQ9FH80.
PRIDEiQ9FH80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45300.1; AT5G45300.1; AT5G45300. [Q9FH80-1]
GeneIDi834566.
GrameneiAT5G45300.1; AT5G45300.1; AT5G45300.
KEGGiath:AT5G45300.

Organism-specific databases

TAIRiAT5G45300.

Phylogenomic databases

eggNOGiENOG410IMY0. Eukaryota.
ENOG410YA7C. LUCA.
HOGENOMiHOG000238755.
InParanoidiQ9FH80.
OMAiMAFHEYG.
OrthoDBiEOG093604F0.
PhylomeDBiQ9FH80.

Miscellaneous databases

PROiQ9FH80.

Gene expression databases

GenevisibleiQ9FH80. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR008540. BES1_N.
IPR001554. Glyco_hydro_14.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF05687. BES1_N. 1 hit.
PF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBAM8_ARATH
AccessioniPrimary (citable) accession number: Q9FH80
Secondary accession number(s): Q2V314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.