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Q9FH80 (BAM8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-amylase 8
EC=3.2.1.2
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 2
Gene names
Name:BAM8
Synonyms:BMY2
Ordered Locus Names:At5g45300
ORF Names:K9E15.8
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 14 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FH80-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FH80-2)

The sequence of this isoform differs from the canonical sequence as follows:
     160-161: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Beta-amylase 8
PRO_0000393422

Regions

Region624 – 6252Substrate binding By similarity

Sites

Active site4291Proton donor By similarity
Active site6231Proton acceptor By similarity
Binding site2961Substrate By similarity
Binding site3361Substrate By similarity
Binding site5371Substrate By similarity

Natural variations

Alternative sequence160 – 1612Missing in isoform 2.
VSP_038980

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: D591C143B130EC7B

FASTA68977,160
        10         20         30         40         50         60 
MHTLNNTITT TTGSQDPNLD PIPDPDQFPN RNRNQPQSRR PRGFAAAAAA ASIAPTENDV 

        70         80         90        100        110        120 
NNGNIAGIGG GEGSSGGGGG GGGKGKRERE KEKERTKLRE RHRRAITSRM LAGLRQYGNF 

       130        140        150        160        170        180 
PLPARADMND VIAALAREAG WSVEADGTTY RQSQQPNHVV QFPTRSIESP LSSSTLKNCA 

       190        200        210        220        230        240 
KAAIESQQHS VLRNDEKLAP VSLDSIGIAE SDHPGNGRYT SVSPITSVGC LEANQLIQDV 

       250        260        270        280        290        300 
HSAEQCNDFT ESFYVPVYAM LPVGIIDNFG QLVDPEGVRQ ELSYMKSLNV DGVVIDCWWG 

       310        320        330        340        350        360 
IVEGWNPQKY VWSGYRELFN LIRDFKLKLQ VVMAFHEYGG NASGNVMISL PQWVLKIGKD 

       370        380        390        400        410        420 
NPDIFFTDRE GRRSFECLNW SIDKERVLHG RTGIEVYFDF MRSFRSEFDD LFVEGLITAV 

       430        440        450        460        470        480 
EIGLGASGEL KYPSFPERMG WIYPGIGEFQ CYDKYSQLSL QKEAKSRGFT FWGKGPENAG 

       490        500        510        520        530        540 
QYSSHPHETV FFQERGEYDS YYGRFFLNWY SQLLIGHAEN VLSLANLAFE ETKIIVKIPA 

       550        560        570        580        590        600 
IYWSYKTASH AAELTAGYYN PSNRDGYSLV FETLKKYSVT VKFVCPGPQM SPNAHEEALA 

       610        620        630        640        650        660 
DPEGLSWQVI NAAWDKGLQI GGENAITCFD RDGCMRLIDI AKPRNHPDGY HFSFFTYRQP 

       670        680 
SPLVQGSTCF PDLDYFIKRM HGDIRDKQF

« Hide

Isoform 2 [UniParc].

Checksum: 2E718FDFDF70EDAC
Show »

FASTA68776,933

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts."
Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C.
Plant Cell 20:1040-1058(2008) [PubMed: 18390594] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB020744 Genomic DNA. Translation: BAB10251.1.
CP002688 Genomic DNA. Translation: AED95229.1.
CP002688 Genomic DNA. Translation: AED95230.1.
AK117140 mRNA. Translation: BAC41818.1.
BT006482 mRNA. Translation: AAP21290.1.
IPIIPI00517397.
IPI00656647.
RefSeqNP_001032014.1. NM_001036937.1.
NP_199343.1. NM_123898.2.
UniGeneAt.30019.

3D structure databases

HSSPHSSP built from PDB template 1FA2 based on UniProtKB P10537.
ProteinModelPortalQ9FH80.
SMRQ9FH80. Positions 252-684.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9FH80. 1 interaction.

Protein family/group databases

CAZyGH14. Glycoside Hydrolase Family 14.

Proteomic databases

PRIDEQ9FH80.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G45300.1; AT5G45300.1; AT5G45300.
GeneID834566.
GenomeReviewsGene locus AT5G45300 in contig BA000015_GR.
KEGGath:AT5G45300.
NMPDRfig|3702.1.peg.26304.

Organism-specific databases

TAIRAt5g45300.

Phylogenomic databases

GeneTreeEPGT00070000029015.
HOGENOMHBG747772.
InParanoidQ9FH80.
OMAVVMAFHE.
PhylomeDBQ9FH80.
ProtClustDBPLN02705.

Gene expression databases

GenevestigatorQ9FH80.

Family and domain databases

InterProIPR008540. BZR1.
IPR001554. Glyco_hydro_14.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF05687. DUF822. 1 hit.
PF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSPR00750. BETAAMYLASE.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00506. BETA_AMYLASE_1. False negative.
PS00679. BETA_AMYLASE_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBAM8_ARATH
AccessionPrimary (citable) accession number: Q9FH80
Secondary accession number(s): Q2V314
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 1, 2001
Last modified: January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents