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Q9FH76 (ABAH3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Abscisic acid 8'-hydroxylase 3

Short name=ABA 8'-hydroxylase 3
EC=1.14.13.93
Alternative name(s):
Cytochrome P450 707A3
Gene names
Name:CYP707A3
Ordered Locus Names:At5g45340
ORF Names:K9E15.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of postgermination growth. Ref.1 Ref.5 Ref.6

Catalytic activity

+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.

Cofactor

Heme group By similarity.

Enzyme regulation

Inhibited by tetcyclcis, but not by metyrapone. Ref.5

Pathway

Plant hormone degradation; abscisic acid degradation.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Mainly expressed in flower buds, flowers, rosette leaves and roots. Lower expression in mature siliques and inflorescence stems. Not expressed in dry seeds. Ref.1 Ref.5

Developmental stage

Up-regulated 12 hours after imbibition. Ref.5

Induction

By abscisic acid, brassinosteroid or gibberellin treatments, by salt or osmotic stresses, and by dehydration and rehydration. Expression regulated by phytochrome B. Ref.1 Ref.5 Ref.6 Ref.7

Disruption phenotype

Plants are not affected in seed germination, but show a restricted greening rate after germination and increase the drought resistance. Ref.6

Sequence similarities

Belongs to the cytochrome P450 family.

Biophysicochemical properties

Kinetic parameters:

KM=1.3 µM for (+)-ABA Ref.1

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FH76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FH76-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-447: WSIVGPSDGIQYGP → LVHLQNDNSPFGN
     448-463: Missing.
Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463Abscisic acid 8'-hydroxylase 3
PRO_0000288641

Regions

Transmembrane6 – 2621Helical; Potential

Sites

Metal binding4111Iron (heme axial ligand) By similarity

Natural variations

Alternative sequence434 – 44714WSIVG…IQYGP → LVHLQNDNSPFGN in isoform 2.
VSP_025739
Alternative sequence448 – 46316Missing in isoform 2.
VSP_025740

Experimental info

Mutagenesis4111C → A: Loss of activity. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: CCD17293F553F812

FASTA46352,367
        10         20         30         40         50         60 
MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF QLYSQDPNVF 

        70         80         90        100        110        120 
FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL FKPTFPASKE RMLGKQAIFF 

       130        140        150        160        170        180 
HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE SIAQESLNSW DGTQLNTYQE MKTYTFNVAL 

       190        200        210        220        230        240 
ISILGKDEVY YREDLKRCYY ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR 

       250        260        270        280        290        300 
QNPSSHTDLL GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA 

       310        320        330        340        350        360 
VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA VEDVEYEGYL 

       370        380        390        400        410        420 
IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT FMPFGSGIHS CPGNELAKLE 

       430        440        450        460 
ISVLIHHLTT KYRWSIVGPS DGIQYGPFAL PQNGLPIALE RKP 

« Hide

Isoform 2 [UniParc].

Checksum: 281DA0613C096FD3
Show »

FASTA44650,599

References

« Hide 'large scale' references
[1]"Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid."
Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., Mizutani M.
Plant Physiol. 134:1439-1449(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism."
Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.
EMBO J. 23:1647-1656(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, FUNCTION, MUTAGENESIS OF CYS-411, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[6]"CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana."
Umezawa T., Okamoto M., Kushiro T., Nambara E., Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y., Shinozaki K.
Plant J. 46:171-182(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
[7]"Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism."
Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E.
Plant J. 48:354-366(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY PHYTOCHROME B.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB122150 mRNA. Translation: BAD16630.1.
AB020744 Genomic DNA. Translation: BAB10255.1.
CP002688 Genomic DNA. Translation: AED95234.1.
CP002688 Genomic DNA. Translation: AED95235.1.
AY065065 mRNA. Translation: AAL57698.1.
AY102136 mRNA. Translation: AAM26703.1.
RefSeqNP_199347.2. NM_123902.2.
NP_851136.1. NM_180805.3.
UniGeneAt.9625.

3D structure databases

ProteinModelPortalQ9FH76.
SMRQ9FH76. Positions 34-459.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ9FH76.
ChEMBLCHEMBL4538.

Proteomic databases

PRIDEQ9FH76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G45340.1; AT5G45340.1; AT5G45340. [Q9FH76-1]
GeneID834570.
KEGGath:AT5G45340.

Organism-specific databases

GeneFarm1253. 94.
TAIRAT5G45340.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000237614.
InParanoidQ9FH76.
KOK09843.
OMAAVIGHLM.
PhylomeDBQ9FH76.
ProtClustDBPLN02196.

Enzyme and pathway databases

BioCycARA:AT5G45340-MONOMER.
ARA:GQT-1434-MONOMER.
MetaCyc:AT5G45340-MONOMER.
SABIO-RKQ9FH76.
UniPathwayUPA00093.

Gene expression databases

GenevestigatorQ9FH76.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABAH3_ARATH
AccessionPrimary (citable) accession number: Q9FH76
Secondary accession number(s): Q3E8G7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names