Reviewed, UniProtKB/Swiss-Prot Q9FH76 (ABAH3_ARATH)

Names and origin

Protein names

Recommended name:
    Abscisic acid 8'-hydroxylase 3
      Short name=ABA 8'-hydroxylase 3
    EC=1.14.13.93
Alternative name(s):
    Cytochrome P450 707A3

Gene names

Name:	CYP707A3
Ordered Locus Names:	At5g45340
ORF Names:	K9E15.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of postgermination growth. Ref.1 Ref.4 Ref.5
Catalytic activity	+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Enzyme regulation	Inhibited by tetcyclcis, but not by metyrapone. Ref.4
Pathway	Plant hormone degradation; abscisic acid degradation.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Mainly expressed in flower buds, flowers, rosette leaves and roots. Lower expression in mature siliques and inflorescence stems. Not expressed in dry seeds. Ref.1 Ref.4
Developmental stage	Up-regulated 12 hours after imbibition. Ref.4
Induction	By abscisic acid, brassinosteroid or gibberellin treatments, by salt or osmotic stresses, and by dehydration and rehydration. Expression regulated by phytochrome B. Ref.1 Ref.4 Ref.5 Ref.6
Disruption phenotype	Plants are not affected in seed germination, but show a restricted greening rate after germination and increase the drought resistance. Ref.5
Sequence similarities	Belongs to the cytochrome P450 family.
Biophysicochemical properties	Kinetic parameters: KM=1.3 µM for (+)-ABA

Ontologies

Keywords
Biological process	Stress response
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	abscisic acid catabolic process Ref.5 Traceable author statement. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to red or far red light Ref.6 Inferred from expression pattern. Source: TAIR response to water deprivation Ref.5 Inferred from mutant phenotype. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	(+)-abscisic acid 8'-hydroxylase activity Ref.1 Inferred from direct assay. Source: TAIR electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FH76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FH76-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-447: WSIVGPSDGIQYGP → LVHLQNDNSPFGN
     448-463: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Abscisic acid 8'-hydroxylase 3		PRO_0000288641
Regions
	Transmembrane	6 – 26	21	Potential
Sites
	Metal binding	411	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	434 – 447	14	WSIVG…IQYGP → LVHLQNDNSPFGN in isoform 2.		VSP_025739
	Alternative sequence	448 – 463	16	Missing in isoform 2.		VSP_025740
Experimental info
	Mutagenesis	411	1	C → A: Loss of activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: CCD17293F553F812	FASTA	463	52,367
	10 20 30 40 50 60 MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF QLYSQDPNVF 70 80 90 100 110 120 FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL FKPTFPASKE RMLGKQAIFF 130 140 150 160 170 180 HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE SIAQESLNSW DGTQLNTYQE MKTYTFNVAL 190 200 210 220 230 240 ISILGKDEVY YREDLKRCYY ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR 250 260 270 280 290 300 QNPSSHTDLL GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA 310 320 330 340 350 360 VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA VEDVEYEGYL 370 380 390 400 410 420 IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT FMPFGSGIHS CPGNELAKLE 430 440 450 460 ISVLIHHLTT KYRWSIVGPS DGIQYGPFAL PQNGLPIALE RKP
	Isoform 2. Checksum: 281DA0613C096FD3	FASTA	446	50,599
	10 20 30 40 50 60 MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF QLYSQDPNVF 70 80 90 100 110 120 FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL FKPTFPASKE RMLGKQAIFF 130 140 150 160 170 180 HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE SIAQESLNSW DGTQLNTYQE MKTYTFNVAL 190 200 210 220 230 240 ISILGKDEVY YREDLKRCYY ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR 250 260 270 280 290 300 QNPSSHTDLL GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA 310 320 330 340 350 360 VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA VEDVEYEGYL 370 380 390 400 410 420 IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT FMPFGSGIHS CPGNELAKLE 430 440 ISVLIHHLTT KYRLVHLQND NSPFGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid." Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., Mizutani M. Plant Physiol. 134:1439-1449(2004) [PubMed: 15064374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism." Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E. EMBO J. 23:1647-1656(2004) [PubMed: 15044947] [Abstract] Cited for: IDENTIFICATION, FUNCTION, MUTAGENESIS OF CYS-411, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[5]	"CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana." Umezawa T., Okamoto M., Kushiro T., Nambara E., Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y., Shinozaki K. Plant J. 46:171-182(2006) [PubMed: 16623881] [Abstract] Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
[6]	"Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism." Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E. Plant J. 48:354-366(2006) [PubMed: 17010113] [Abstract] Cited for: INDUCTION BY PHYTOCHROME B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB122150 mRNA. Translation: BAD16630.1. AB020744 Genomic DNA. Translation: BAB10255.1. AY065065 mRNA. Translation: AAL57698.1. AY102136 mRNA. Translation: AAM26703.1.
IPI	IPI00521616. IPI00541379.
RefSeq	NP_199347.2. NP_851136.1.
UniGene	At.9625
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	834570.
GenomeReviews	Gene locus AT5G45340 in contig BA000015_GR.
KEGG	ath:AT5G45340.
NMPDR	fig|3702.1.peg.26309.
Organism-specific databases
GeneFarm	1253. 94.
TAIR	At5g45340.
Phylogenomic databases
OMA	Q9FH76. HIESIAQ.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G45340-MON.
BRENDA	1.14.13.93. 302.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABAH3_ARATH

Accession

Primary (citable) accession number: Q9FH76
Secondary accession number(s): Q3E8G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)