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Protein

Abscisic acid 8'-hydroxylase 3

Gene

CYP707A3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of postgermination growth.3 Publications

Catalytic activityi

+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibited by tetcyclcis, but not by metyrapone.1 Publication

Kineticsi

  1. KM=1.3 µM for (+)-ABA1 Publication

    Pathway:iabscisic acid degradation

    This protein is involved in the pathway abscisic acid degradation, which is part of Plant hormone degradation.
    View all proteins of this organism that are known to be involved in the pathway abscisic acid degradation and in Plant hormone degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi411 – 4111Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    • (+)-abscisic acid 8'-hydroxylase activity Source: TAIR
    • heme binding Source: InterPro
    • iron ion binding Source: InterPro

    GO - Biological processi

    • abscisic acid catabolic process Source: TAIR
    • response to red or far red light Source: TAIR
    • response to water deprivation Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT5G45340-MONOMER.
    ARA:GQT-1434-MONOMER.
    MetaCyc:AT5G45340-MONOMER.
    BRENDAi1.14.13.93. 399.
    ReactomeiREACT_311425. RA biosynthesis pathway.
    REACT_335713. Vitamins.
    SABIO-RKQ9FH76.
    UniPathwayiUPA00093.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Abscisic acid 8'-hydroxylase 3 (EC:1.14.13.93)
    Short name:
    ABA 8'-hydroxylase 3
    Alternative name(s):
    Cytochrome P450 707A3
    Gene namesi
    Name:CYP707A3
    Ordered Locus Names:At5g45340
    ORF Names:K9E15.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G45340.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants are not affected in seed germination, but show a restricted greening rate after germination and increase the drought resistance.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi411 – 4111C → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Abscisic acid 8'-hydroxylase 3PRO_0000288641Add
    BLAST

    Proteomic databases

    PRIDEiQ9FH76.

    Expressioni

    Tissue specificityi

    Mainly expressed in flower buds, flowers, rosette leaves and roots. Lower expression in mature siliques and inflorescence stems. Not expressed in dry seeds.2 Publications

    Developmental stagei

    Up-regulated 12 hours after imbibition.1 Publication

    Inductioni

    By abscisic acid, brassinosteroid or gibberellin treatments, by salt or osmotic stresses, and by dehydration and rehydration. Expression regulated by phytochrome B.4 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G45340.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FH76.
    SMRiQ9FH76. Positions 34-461.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000237614.
    KOiK09843.
    OMAiWTADVIQ.
    PhylomeDBiQ9FH76.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9FH76-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF
    60 70 80 90 100
    QLYSQDPNVF FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL
    110 120 130 140 150
    FKPTFPASKE RMLGKQAIFF HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE
    160 170 180 190 200
    SIAQESLNSW DGTQLNTYQE MKTYTFNVAL ISILGKDEVY YREDLKRCYY
    210 220 230 240 250
    ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR QNPSSHTDLL
    260 270 280 290 300
    GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA
    310 320 330 340 350
    VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA
    360 370 380 390 400
    VEDVEYEGYL IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT
    410 420 430 440 450
    FMPFGSGIHS CPGNELAKLE ISVLIHHLTT KYRWSIVGPS DGIQYGPFAL
    460
    PQNGLPIALE RKP
    Length:463
    Mass (Da):52,367
    Last modified:March 1, 2001 - v1
    Checksum:iCCD17293F553F812
    GO
    Isoform 2 (identifier: Q9FH76-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         434-447: WSIVGPSDGIQYGP → LVHLQNDNSPFGN
         448-463: Missing.

    Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
    Show »
    Length:446
    Mass (Da):50,599
    Checksum:i281DA0613C096FD3
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei434 – 44714WSIVG…IQYGP → LVHLQNDNSPFGN in isoform 2. CuratedVSP_025739Add
    BLAST
    Alternative sequencei448 – 46316Missing in isoform 2. CuratedVSP_025740Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB122150 mRNA. Translation: BAD16630.1.
    AB020744 Genomic DNA. Translation: BAB10255.1.
    CP002688 Genomic DNA. Translation: AED95234.1.
    CP002688 Genomic DNA. Translation: AED95235.1.
    AY065065 mRNA. Translation: AAL57698.1.
    AY102136 mRNA. Translation: AAM26703.1.
    RefSeqiNP_199347.2. NM_123902.2. [Q9FH76-2]
    NP_851136.1. NM_180805.3. [Q9FH76-1]
    UniGeneiAt.9625.

    Genome annotation databases

    EnsemblPlantsiAT5G45340.1; AT5G45340.1; AT5G45340. [Q9FH76-1]
    GeneIDi834570.
    KEGGiath:AT5G45340.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB122150 mRNA. Translation: BAD16630.1.
    AB020744 Genomic DNA. Translation: BAB10255.1.
    CP002688 Genomic DNA. Translation: AED95234.1.
    CP002688 Genomic DNA. Translation: AED95235.1.
    AY065065 mRNA. Translation: AAL57698.1.
    AY102136 mRNA. Translation: AAM26703.1.
    RefSeqiNP_199347.2. NM_123902.2. [Q9FH76-2]
    NP_851136.1. NM_180805.3. [Q9FH76-1]
    UniGeneiAt.9625.

    3D structure databases

    ProteinModelPortaliQ9FH76.
    SMRiQ9FH76. Positions 34-461.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G45340.1.

    Chemistry

    BindingDBiQ9FH76.
    ChEMBLiCHEMBL4538.

    Proteomic databases

    PRIDEiQ9FH76.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G45340.1; AT5G45340.1; AT5G45340. [Q9FH76-1]
    GeneIDi834570.
    KEGGiath:AT5G45340.

    Organism-specific databases

    GeneFarmi1253. 94.
    TAIRiAT5G45340.

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000237614.
    KOiK09843.
    OMAiWTADVIQ.
    PhylomeDBiQ9FH76.

    Enzyme and pathway databases

    UniPathwayiUPA00093.
    BioCyciARA:AT5G45340-MONOMER.
    ARA:GQT-1434-MONOMER.
    MetaCyc:AT5G45340-MONOMER.
    BRENDAi1.14.13.93. 399.
    ReactomeiREACT_311425. RA biosynthesis pathway.
    REACT_335713. Vitamins.
    SABIO-RKQ9FH76.

    Miscellaneous databases

    PROiQ9FH76.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid."
      Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., Mizutani M.
      Plant Physiol. 134:1439-1449(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism."
      Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.
      EMBO J. 23:1647-1656(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, FUNCTION, MUTAGENESIS OF CYS-411, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    6. "CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana."
      Umezawa T., Okamoto M., Kushiro T., Nambara E., Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y., Shinozaki K.
      Plant J. 46:171-182(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
    7. "Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism."
      Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E.
      Plant J. 48:354-366(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY PHYTOCHROME B.

    Entry informationi

    Entry nameiABAH3_ARATH
    AccessioniPrimary (citable) accession number: Q9FH76
    Secondary accession number(s): Q3E8G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: March 1, 2001
    Last modified: July 22, 2015
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.