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Q9FH76

- ABAH3_ARATH

UniProt

Q9FH76 - ABAH3_ARATH

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Protein
Abscisic acid 8'-hydroxylase 3
Gene
CYP707A3, At5g45340, K9E15.12
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of postgermination growth.3 Publications

Catalytic activityi

+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.

Cofactori

Heme group By similarity.

Enzyme regulationi

Inhibited by tetcyclcis, but not by metyrapone.1 Publication

Kineticsi

  1. KM=1.3 µM for (+)-ABA1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi411 – 4111Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. (+)-abscisic acid 8'-hydroxylase activity Source: TAIR
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. abscisic acid catabolic process Source: TAIR
  2. response to red or far red light Source: TAIR
  3. response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G45340-MONOMER.
ARA:GQT-1434-MONOMER.
MetaCyc:AT5G45340-MONOMER.
SABIO-RKQ9FH76.
UniPathwayiUPA00093.

Names & Taxonomyi

Protein namesi
Recommended name:
Abscisic acid 8'-hydroxylase 3 (EC:1.14.13.93)
Short name:
ABA 8'-hydroxylase 3
Alternative name(s):
Cytochrome P450 707A3
Gene namesi
Name:CYP707A3
Ordered Locus Names:At5g45340
ORF Names:K9E15.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G45340.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Plants are not affected in seed germination, but show a restricted greening rate after germination and increase the drought resistance.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi411 – 4111C → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Abscisic acid 8'-hydroxylase 3
PRO_0000288641Add
BLAST

Proteomic databases

PRIDEiQ9FH76.

Expressioni

Tissue specificityi

Mainly expressed in flower buds, flowers, rosette leaves and roots. Lower expression in mature siliques and inflorescence stems. Not expressed in dry seeds.2 Publications

Developmental stagei

Up-regulated 12 hours after imbibition.1 Publication

Inductioni

By abscisic acid, brassinosteroid or gibberellin treatments, by salt or osmotic stresses, and by dehydration and rehydration. Expression regulated by phytochrome B.4 Publications

Gene expression databases

GenevestigatoriQ9FH76.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ9FH76.
SMRiQ9FH76. Positions 34-461.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000237614.
InParanoidiQ9FH76.
KOiK09843.
OMAiILANEEP.
PhylomeDBiQ9FH76.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FH76-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF    50
QLYSQDPNVF FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL 100
FKPTFPASKE RMLGKQAIFF HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE 150
SIAQESLNSW DGTQLNTYQE MKTYTFNVAL ISILGKDEVY YREDLKRCYY 200
ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR QNPSSHTDLL 250
GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA 300
VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA 350
VEDVEYEGYL IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT 400
FMPFGSGIHS CPGNELAKLE ISVLIHHLTT KYRWSIVGPS DGIQYGPFAL 450
PQNGLPIALE RKP 463
Length:463
Mass (Da):52,367
Last modified:March 1, 2001 - v1
Checksum:iCCD17293F553F812
GO
Isoform 2 (identifier: Q9FH76-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-447: WSIVGPSDGIQYGP → LVHLQNDNSPFGN
     448-463: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Show »
Length:446
Mass (Da):50,599
Checksum:i281DA0613C096FD3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei434 – 44714WSIVG…IQYGP → LVHLQNDNSPFGN in isoform 2.
VSP_025739Add
BLAST
Alternative sequencei448 – 46316Missing in isoform 2.
VSP_025740Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB122150 mRNA. Translation: BAD16630.1.
AB020744 Genomic DNA. Translation: BAB10255.1.
CP002688 Genomic DNA. Translation: AED95234.1.
CP002688 Genomic DNA. Translation: AED95235.1.
AY065065 mRNA. Translation: AAL57698.1.
AY102136 mRNA. Translation: AAM26703.1.
RefSeqiNP_199347.2. NM_123902.2. [Q9FH76-2]
NP_851136.1. NM_180805.3. [Q9FH76-1]
UniGeneiAt.9625.

Genome annotation databases

EnsemblPlantsiAT5G45340.1; AT5G45340.1; AT5G45340. [Q9FH76-1]
GeneIDi834570.
KEGGiath:AT5G45340.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB122150 mRNA. Translation: BAD16630.1 .
AB020744 Genomic DNA. Translation: BAB10255.1 .
CP002688 Genomic DNA. Translation: AED95234.1 .
CP002688 Genomic DNA. Translation: AED95235.1 .
AY065065 mRNA. Translation: AAL57698.1 .
AY102136 mRNA. Translation: AAM26703.1 .
RefSeqi NP_199347.2. NM_123902.2. [Q9FH76-2 ]
NP_851136.1. NM_180805.3. [Q9FH76-1 ]
UniGenei At.9625.

3D structure databases

ProteinModelPortali Q9FH76.
SMRi Q9FH76. Positions 34-461.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q9FH76.
ChEMBLi CHEMBL4538.

Proteomic databases

PRIDEi Q9FH76.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G45340.1 ; AT5G45340.1 ; AT5G45340 . [Q9FH76-1 ]
GeneIDi 834570.
KEGGi ath:AT5G45340.

Organism-specific databases

GeneFarmi 1253. 94.
TAIRi AT5G45340.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000237614.
InParanoidi Q9FH76.
KOi K09843.
OMAi ILANEEP.
PhylomeDBi Q9FH76.

Enzyme and pathway databases

UniPathwayi UPA00093 .
BioCyci ARA:AT5G45340-MONOMER.
ARA:GQT-1434-MONOMER.
MetaCyc:AT5G45340-MONOMER.
SABIO-RK Q9FH76.

Gene expression databases

Genevestigatori Q9FH76.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid."
    Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., Mizutani M.
    Plant Physiol. 134:1439-1449(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism."
    Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.
    EMBO J. 23:1647-1656(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION, MUTAGENESIS OF CYS-411, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  6. "CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana."
    Umezawa T., Okamoto M., Kushiro T., Nambara E., Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y., Shinozaki K.
    Plant J. 46:171-182(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
  7. "Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism."
    Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E.
    Plant J. 48:354-366(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY PHYTOCHROME B.

Entry informationi

Entry nameiABAH3_ARATH
AccessioniPrimary (citable) accession number: Q9FH76
Secondary accession number(s): Q3E8G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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