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Q9FH03 (BGL12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 12

Short name=AtBGLU12
EC=3.2.1.21
Gene names
Name:BGLU12
Ordered Locus Names:At5g42260
ORF Names:K5J14.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 22430844. Source: TAIR

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 507485Beta-glucosidase 12
PRO_0000389575

Regions

Region466 – 4672Substrate binding By similarity

Sites

Active site2001Proton donor By similarity
Active site4141Nucleophile By similarity
Binding site501Substrate By similarity
Binding site1541Substrate By similarity
Binding site1991Substrate By similarity
Binding site3441Substrate By similarity
Binding site4591Substrate By similarity

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation2261N-linked (GlcNAc...) Potential
Glycosylation3581N-linked (GlcNAc...) Potential
Disulfide bond219 ↔ 227 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FH03 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 7F7197A3D50D45C4

FASTA50756,966
        10         20         30         40         50         60 
MRTIYLSLLV FIIVLALNEV MAKKHSSTPK LRRSDFPEDF IFGAATSAYQ VEGAAHEDGR 

        70         80         90        100        110        120 
GPSIWDTFSE KYPEKIKDGS NGSIASDSYH LYKEDVGLLH QIGFDAYRFS ISWSRILPRE 

       130        140        150        160        170        180 
NLKGGINQAG IDYYNNLINE LLSKGIKPFA TIFHWDTPQS LEDAYGGFLG AEIVNDFRDY 

       190        200        210        220        230        240 
ADICFKNFGD RVKHWMTLNE PLTVVQQGYV AGVMAPGRCS KFTNPNCTAG NGATEPYIVG 

       250        260        270        280        290        300 
HNLILAHGEA VKVYREKYKA SQKGQVGIAL NAGWNLPYSE SAEDRLAAAR AMAFTFDYFM 

       310        320        330        340        350        360 
EPLVTGKYPI DMVNYVKGGR LPTFTAKQSK MLKGSYDFIG RNYYSSSYAK DVPCSSENVT 

       370        380        390        400        410        420 
LFSDPCASVT GEREGVPIGP KAASDWLLIY PKGIRDLLLY AKYKFKDPVM YITENGRDEA 

       430        440        450        460        470        480 
STGKIDLKDS ERIDYYAQHL KMVQDAISIG ANVKGFFAWS LLDNFEWATG YAVRFGLVYV 

       490        500 
DFNGGRKRYP KKSAKWFKKL LNEKKKN 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023032 Genomic DNA. Translation: BAB10199.1.
CP002688 Genomic DNA. Translation: AED94789.1.
DQ056704 mRNA. Translation: AAY78850.1.
RefSeqNP_199041.1. NM_123591.1.
UniGeneAt.55316.

3D structure databases

ProteinModelPortalQ9FH03.
SMRQ9FH03. Positions 29-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G26140.1-P.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEQ9FH03.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G42260.1; AT5G42260.1; AT5G42260.
GeneID834231.
KEGGath:AT5G42260.

Organism-specific databases

TAIRAT5G42260.

Phylogenomic databases

HOGENOMHOG000088630.
InParanoidQ9FH03.
KOK01188.
OMASAKWFKK.
PhylomeDBQ9FH03.

Enzyme and pathway databases

BioCycARA:AT5G42260-MONOMER.

Gene expression databases

GenevestigatorQ9FH03.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL12_ARATH
AccessionPrimary (citable) accession number: Q9FH03
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names