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Protein

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

Gene

PNG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).By similarity

Catalytic activityi

Hydrolysis of an N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi193ZincBy similarity1
Metal bindingi196ZincBy similarity1
Metal bindingi225ZincBy similarity1
Metal bindingi228ZincBy similarity1
Active sitei251NucleophileBy similarity1
Active sitei278By similarity1
Active sitei295By similarity1

GO - Molecular functioni

GO - Biological processi

  • glycoprotein catabolic process Source: GO_Central
  • protein deglycosylation Source: GO_Central
  • protein quality control for misfolded or incompletely synthesized proteins Source: GO_Central
  • response to microbial phytotoxin Source: TAIR
  • response to ozone Source: TAIR
  • response to salicylic acid Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G49570-MONOMER
ReactomeiR-ATH-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (EC:3.5.1.52)
Alternative name(s):
Peptide:N-glycanase
Short name:
AtPNG1
Gene namesi
Name:PNG1
Ordered Locus Names:At5g49570
ORF Names:K6M13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G49570
TAIRilocus:2157869 AT5G49570

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002489811 – 721Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidaseAdd BLAST721

Proteomic databases

PaxDbiQ9FGY9

PTM databases

iPTMnetiQ9FGY9

Expressioni

Gene expression databases

ExpressionAtlasiQ9FGY9 baseline and differential
GenevisibleiQ9FGY9 AT

Interactioni

Protein-protein interaction databases

BioGridi20265, 1 interactor
STRINGi3702.AT5G49570.1

Structurei

3D structure databases

ProteinModelPortaliQ9FGY9
SMRiQ9FGY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0909 Eukaryota
ENOG410XP69 LUCA
HOGENOMiHOG000285938
InParanoidiQ9FGY9
KOiK01456
OMAiIRSSKWE
OrthoDBiEOG0936037Y
PhylomeDBiQ9FGY9

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR018325 Rad4/PNGase_transGLS-fold
IPR002931 Transglutaminase-like
PfamiView protein in Pfam
PF03835 Rad4, 1 hit
SMARTiView protein in SMART
SM00460 TGc, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FGY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVARKFVVRH EDSSFDVDYN TEDGLEVLRF LIFSLTLVPP EEQKIVAEDD
60 70 80 90 100
NRLVSDESDL ASLSERLRLV SVGEDSVENS DAEMLKSDEE LARMLQAEED
110 120 130 140 150
AIMFQQFVAA RDNGEFEGRI RPYVSQVLMY EDPVRQDAAR KTVPKDELEE
160 170 180 190 200
KALVSLAKEG NFEPSKEERD YAFLLQLLFW FKKSFRWVNE PPCDFCGNKT
210 220 230 240 250
IGQGMGNPLT SELAYGANRV EIYRCTMCPT TTRFPRYNDP LKLVETKKGR
260 270 280 290 300
CGEWANCFTL YCRTFGYDSR LIMDFTDHVW TECYSHSLKR WIHLDPCEGV
310 320 330 340 350
YDKPMLYEKG WNKKLNYVIA ISKDGVCDVT KRYTKKWHEV LSRRTLTTES
360 370 380 390 400
SLQDGLRTLT RERRRSLMFE SLSKLELRDR NEQEELERNL HSADNASVSL
410 420 430 440 450
PGRQSGDREW RIMRSEFGSD ENSSVSSSSC PVRKCVDDHV TNIYDSFLPI
460 470 480 490 500
LTQFVEDGLP VARTNEVLKM IKQVLVDLKN APYKTRKARL TLDSDNSSSF
510 520 530 540 550
PEQFLPALGD LLLALSLKSE RDTNGKSVTI SVDGKLTKTA IALPVALDAL
560 570 580 590 600
RELVADLSKY QNLNKDSLSF PLVKQNRVCS GSVLASGEEL PSGIATAAFD
610 620 630 640 650
GIQESKWEEP NGAKGCWIVY KTLYNQMHQL IAYELMSAND APERDPKDWI
660 670 680 690 700
LEGSNDGGST WCVLDKQTSQ VFEERFQRKS YKITTPGFQA NLFRFRFLSV
710 720
RDVNSTSRLQ LGSIDLYRSH Q
Length:721
Mass (Da):82,446
Last modified:March 1, 2001 - v1
Checksum:iBD516DD961C0CC91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti346L → P in AAO24593 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023033 Genomic DNA Translation: BAB10770.1
CP002688 Genomic DNA Translation: AED95831.1
AY140065 mRNA Translation: AAM98206.1
BT003161 mRNA Translation: AAO24593.1
BT003398 mRNA Translation: AAO30061.1
AK228156 mRNA Translation: BAF00112.1
RefSeqiNP_199768.1, NM_124335.3
UniGeneiAt.27656
At.29778

Genome annotation databases

EnsemblPlantsiAT5G49570.1; AT5G49570.1; AT5G49570
GeneIDi835019
GrameneiAT5G49570.1; AT5G49570.1; AT5G49570
KEGGiath:AT5G49570

Similar proteinsi

Entry informationi

Entry nameiPNG1_ARATH
AccessioniPrimary (citable) accession number: Q9FGY9
Secondary accession number(s): Q84WJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health