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Protein

Beta-D-xylosidase 1

Gene

BXL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pectic arabinan modification in mucilage secretory cells. Acts also as a beta-D-xylosidase during the remodeling of xylans in vascular development.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=1.2 mM for p-nitrophenyl-beta-D-xylopyranoside1 Publication

    pH dependencei

    Optimum pH is 4.9.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei296 – 2961By similarity

    GO - Molecular functioni

    • alpha-L-arabinofuranosidase activity Source: TAIR

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • seed coat development Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G49360-MONOMER.
    BRENDAi3.2.1.37. 399.
    SABIO-RKQ9FGY1.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 1 (EC:3.2.1.-)
    Short name:
    AtBXL1
    Alternative name(s):
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Gene namesi
    Name:BXL1
    Synonyms:XYL1
    Ordered Locus Names:At5g49360
    ORF Names:K7J8.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G49360.

    Subcellular locationi

    GO - Cellular componenti

    • apoplast Source: TAIR
    • plant-type cell wall Source: TAIR
    • proteinaceous extracellular matrix Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Delayed seeds germination resulting from an altered mucilage composition. No visible growth defects; probably due to partial redundancy with BXL2. Bxl1 and bxl2 double mutants have shortened siliques and curled leaf edges.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 774744Beta-D-xylosidase 1PRO_0000384056Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi658 – 6581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9FGY1.
    PRIDEiQ9FGY1.

    Expressioni

    Tissue specificityi

    Expressed in leaves, stems, seedlings, roots, inflorescences, siliques and developing seeds. Expressed in the vasculature of the roots, leaves, flowers and silique. Expressed in tissues undergoing secondary cell wall thickening such as protoxylem, metaxylem, intrafascicular cambium and fibers.3 Publications

    Inductioni

    By sugar starvation.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi20242. 2 interactions.
    STRINGi3702.AT5G49360.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FGY1.
    SMRiQ9FGY1. Positions 59-769.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1472.
    HOGENOMiHOG000031216.
    InParanoidiQ9FGY1.
    OMAiPYANLGP.
    PhylomeDBiQ9FGY1.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FGY1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCYNKALLI GNKVVVILVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC
    60 70 80 90 100
    RANVPIHVRV QDLLGRLTLQ EKIRNLVNNA AAVPRLGIGG YEWWSEALHG
    110 120 130 140 150
    ISDVGPGAKF GGAFPGATSF PQVITTAASF NQSLWEEIGR VVSDEARAMY
    160 170 180 190 200
    NGGVAGLTYW SPNVNILRDP RWGRGQETPG EDPIVAAKYA ASYVRGLQGT
    210 220 230 240 250
    AAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTQQDL EDTYNVPFKS
    260 270 280 290 300
    CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV
    310 320 330 340 350
    DVFFNQQHYT STPEEAAARS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND
    360 370 380 390 400
    INLALANTLT VQMRLGMFDG NLGPYANLGP RDVCTPAHKH LALEAAHQGI
    410 420 430 440 450
    VLLKNSARSL PLSPRRHRTV AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG
    460 470 480 490 500
    ISRYARTLHQ AGCAGVACKG NQGFGAAEAA AREADATVLV MGLDQSIEAE
    510 520 530 540 550
    TRDRTGLLLP GYQQDLVTRV AQASRGPVIL VLMSGGPIDV TFAKNDPRVA
    560 570 580 590 600
    AIIWAGYPGQ AGGAAIANII FGAANPGGKL PMTWYPQDYV AKVPMTVMAM
    610 620 630 640 650
    RASGNYPGRT YRFYKGPVVF PFGFGLSYTT FTHSLAKSPL AQLSVSLSNL
    660 670 680 690 700
    NSANTILNSS SHSIKVSHTN CNSFPKMPLH VEVSNTGEFD GTHTVFVFAE
    710 720 730 740 750
    PPINGIKGLG VNKQLIAFEK VHVMAGAKQT VQVDVDACKH LGVVDEYGKR
    760 770
    RIPMGEHKLH IGDLKHTILV QPQL
    Length:774
    Mass (Da):83,524
    Last modified:March 1, 2001 - v1
    Checksum:i77478F0F28A5DA51
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB023034 Genomic DNA. Translation: BAB09906.1.
    CP002688 Genomic DNA. Translation: AED95802.1.
    AY120767 mRNA. Translation: AAM53325.1.
    RefSeqiNP_199747.1. NM_124313.2.
    UniGeneiAt.22961.
    At.28192.
    At.47570.

    Genome annotation databases

    EnsemblPlantsiAT5G49360.1; AT5G49360.1; AT5G49360.
    GeneIDi834996.
    KEGGiath:AT5G49360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB023034 Genomic DNA. Translation: BAB09906.1.
    CP002688 Genomic DNA. Translation: AED95802.1.
    AY120767 mRNA. Translation: AAM53325.1.
    RefSeqiNP_199747.1. NM_124313.2.
    UniGeneiAt.22961.
    At.28192.
    At.47570.

    3D structure databases

    ProteinModelPortaliQ9FGY1.
    SMRiQ9FGY1. Positions 59-769.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi20242. 2 interactions.
    STRINGi3702.AT5G49360.1.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Proteomic databases

    PaxDbiQ9FGY1.
    PRIDEiQ9FGY1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G49360.1; AT5G49360.1; AT5G49360.
    GeneIDi834996.
    KEGGiath:AT5G49360.

    Organism-specific databases

    TAIRiAT5G49360.

    Phylogenomic databases

    eggNOGiCOG1472.
    HOGENOMiHOG000031216.
    InParanoidiQ9FGY1.
    OMAiPYANLGP.
    PhylomeDBiQ9FGY1.

    Enzyme and pathway databases

    BioCyciARA:AT5G49360-MONOMER.
    BRENDAi3.2.1.37. 399.
    SABIO-RKQ9FGY1.

    Miscellaneous databases

    PROiQ9FGY1.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
      Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
      Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
      Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
      Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    6. "Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis."
      Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N.
      Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY SUGAR STARVATION.
    7. "AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase required for pectic arabinan modification in arabidopsis mucilage secretory cells."
      Arsovski A.A., Popma T.M., Haughn G.W., Carpita N.C., McCann M.C., Western T.L.
      Plant Physiol. 150:1219-1234(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiBXL1_ARATH
    AccessioniPrimary (citable) accession number: Q9FGY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: March 1, 2001
    Last modified: June 24, 2015
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Might be processed at the C-terminus.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.