Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9FGY1 (BXL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-D-xylosidase 1

Short name=AtBXL1
EC=3.2.1.-
Alternative name(s):
Alpha-L-arabinofuranosidase
EC=3.2.1.55
Gene names
Name:BXL1
Synonyms:XYL1
Ordered Locus Names:At5g49360
ORF Names:K7J8.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length774 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pectic arabinan modification in mucilage secretory cells. Acts also as a beta-D-xylosidase during the remodeling of xylans in vascular development. Ref.4 Ref.5 Ref.7

Catalytic activity

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. Ref.5

Subcellular location

Secretedextracellular spaceextracellular matrix Probable.

Tissue specificity

Expressed in leaves, stems, seedlings, roots, inflorescences, siliques and developing seeds. Expressed in the vasculature of the roots, leaves, flowers and silique. Expressed in tissues undergoing secondary cell wall thickening such as protoxylem, metaxylem, intrafascicular cambium and fibers. Ref.4 Ref.5 Ref.7

Induction

By sugar starvation. Ref.6

Disruption phenotype

Delayed seeds germination resulting from an altered mucilage composition. No visible growth defects; probably due to partial redundancy with BXL2. Bxl1 and bxl2 double mutants have shortened siliques and curled leaf edges. Ref.7

Miscellaneous

Might be processed at the C-terminus.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family.

Biophysicochemical properties

Kinetic parameters:

KM=1.2 mM for p-nitrophenyl-beta-D-xylopyranoside Ref.5

pH dependence:

Optimum pH is 4.9.

Temperature dependence:

Optimum temperature is 55 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 774744Beta-D-xylosidase 1
PRO_0000384056

Sites

Active site2961 By similarity

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation6581N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9FGY1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 77478F0F28A5DA51

FASTA77483,524
        10         20         30         40         50         60 
MSCYNKALLI GNKVVVILVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC RANVPIHVRV 

        70         80         90        100        110        120 
QDLLGRLTLQ EKIRNLVNNA AAVPRLGIGG YEWWSEALHG ISDVGPGAKF GGAFPGATSF 

       130        140        150        160        170        180 
PQVITTAASF NQSLWEEIGR VVSDEARAMY NGGVAGLTYW SPNVNILRDP RWGRGQETPG 

       190        200        210        220        230        240 
EDPIVAAKYA ASYVRGLQGT AAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTQQDL 

       250        260        270        280        290        300 
EDTYNVPFKS CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV 

       310        320        330        340        350        360 
DVFFNQQHYT STPEEAAARS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND INLALANTLT 

       370        380        390        400        410        420 
VQMRLGMFDG NLGPYANLGP RDVCTPAHKH LALEAAHQGI VLLKNSARSL PLSPRRHRTV 

       430        440        450        460        470        480 
AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG ISRYARTLHQ AGCAGVACKG NQGFGAAEAA 

       490        500        510        520        530        540 
AREADATVLV MGLDQSIEAE TRDRTGLLLP GYQQDLVTRV AQASRGPVIL VLMSGGPIDV 

       550        560        570        580        590        600 
TFAKNDPRVA AIIWAGYPGQ AGGAAIANII FGAANPGGKL PMTWYPQDYV AKVPMTVMAM 

       610        620        630        640        650        660 
RASGNYPGRT YRFYKGPVVF PFGFGLSYTT FTHSLAKSPL AQLSVSLSNL NSANTILNSS 

       670        680        690        700        710        720 
SHSIKVSHTN CNSFPKMPLH VEVSNTGEFD GTHTVFVFAE PPINGIKGLG VNKQLIAFEK 

       730        740        750        760        770 
VHVMAGAKQT VQVDVDACKH LGVVDEYGKR RIPMGEHKLH IGDLKHTILV QPQL 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]"Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[6]"Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis."
Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N.
Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY SUGAR STARVATION.
[7]"AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase required for pectic arabinan modification in arabidopsis mucilage secretory cells."
Arsovski A.A., Popma T.M., Haughn G.W., Carpita N.C., McCann M.C., Western T.L.
Plant Physiol. 150:1219-1234(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023034 Genomic DNA. Translation: BAB09906.1.
CP002688 Genomic DNA. Translation: AED95802.1.
AY120767 mRNA. Translation: AAM53325.1.
RefSeqNP_199747.1. NM_124313.2.
UniGeneAt.22961.
At.28192.
At.47570.

3D structure databases

ProteinModelPortalQ9FGY1.
SMRQ9FGY1. Positions 59-769.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20242. 2 interactions.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Proteomic databases

PaxDbQ9FGY1.
PRIDEQ9FGY1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G49360.1; AT5G49360.1; AT5G49360.
GeneID834996.
KEGGath:AT5G49360.

Organism-specific databases

TAIRAT5G49360.

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000031216.
InParanoidQ9FGY1.
OMANSDVTET.
PhylomeDBQ9FGY1.

Enzyme and pathway databases

BioCycARA:AT5G49360-MONOMER.
BRENDA3.2.1.37. 399.
SABIO-RKQ9FGY1.

Gene expression databases

GenevestigatorQ9FGY1.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR30620. PTHR30620. 1 hit.
PfamPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSPR00133. GLHYDRLASE3.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBXL1_ARATH
AccessionPrimary (citable) accession number: Q9FGY1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names