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Q9FGY1

- BXL1_ARATH

UniProt

Q9FGY1 - BXL1_ARATH

Protein

Beta-D-xylosidase 1

Gene

BXL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Involved in pectic arabinan modification in mucilage secretory cells. Acts also as a beta-D-xylosidase during the remodeling of xylans in vascular development.3 Publications

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

    Kineticsi

    1. KM=1.2 mM for p-nitrophenyl-beta-D-xylopyranoside1 Publication

    pH dependencei

    Optimum pH is 4.9.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei296 – 2961By similarity

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. seed coat development Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G49360-MONOMER.
    BRENDAi3.2.1.37. 399.
    SABIO-RKQ9FGY1.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 1 (EC:3.2.1.-)
    Short name:
    AtBXL1
    Alternative name(s):
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Gene namesi
    Name:BXL1
    Synonyms:XYL1
    Ordered Locus Names:At5g49360
    ORF Names:K7J8.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G49360.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. plant-type cell wall Source: TAIR
    3. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Delayed seeds germination resulting from an altered mucilage composition. No visible growth defects; probably due to partial redundancy with BXL2. Bxl1 and bxl2 double mutants have shortened siliques and curled leaf edges.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 774744Beta-D-xylosidase 1PRO_0000384056Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi658 – 6581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9FGY1.
    PRIDEiQ9FGY1.

    Expressioni

    Tissue specificityi

    Expressed in leaves, stems, seedlings, roots, inflorescences, siliques and developing seeds. Expressed in the vasculature of the roots, leaves, flowers and silique. Expressed in tissues undergoing secondary cell wall thickening such as protoxylem, metaxylem, intrafascicular cambium and fibers.3 Publications

    Inductioni

    By sugar starvation.1 Publication

    Gene expression databases

    GenevestigatoriQ9FGY1.

    Interactioni

    Protein-protein interaction databases

    BioGridi20242. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FGY1.
    SMRiQ9FGY1. Positions 59-769.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1472.
    HOGENOMiHOG000031216.
    InParanoidiQ9FGY1.
    OMAiNSDVTET.
    PhylomeDBiQ9FGY1.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FGY1-1 [UniParc]FASTAAdd to Basket

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    MSCYNKALLI GNKVVVILVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC    50
    RANVPIHVRV QDLLGRLTLQ EKIRNLVNNA AAVPRLGIGG YEWWSEALHG 100
    ISDVGPGAKF GGAFPGATSF PQVITTAASF NQSLWEEIGR VVSDEARAMY 150
    NGGVAGLTYW SPNVNILRDP RWGRGQETPG EDPIVAAKYA ASYVRGLQGT 200
    AAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTQQDL EDTYNVPFKS 250
    CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV 300
    DVFFNQQHYT STPEEAAARS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND 350
    INLALANTLT VQMRLGMFDG NLGPYANLGP RDVCTPAHKH LALEAAHQGI 400
    VLLKNSARSL PLSPRRHRTV AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG 450
    ISRYARTLHQ AGCAGVACKG NQGFGAAEAA AREADATVLV MGLDQSIEAE 500
    TRDRTGLLLP GYQQDLVTRV AQASRGPVIL VLMSGGPIDV TFAKNDPRVA 550
    AIIWAGYPGQ AGGAAIANII FGAANPGGKL PMTWYPQDYV AKVPMTVMAM 600
    RASGNYPGRT YRFYKGPVVF PFGFGLSYTT FTHSLAKSPL AQLSVSLSNL 650
    NSANTILNSS SHSIKVSHTN CNSFPKMPLH VEVSNTGEFD GTHTVFVFAE 700
    PPINGIKGLG VNKQLIAFEK VHVMAGAKQT VQVDVDACKH LGVVDEYGKR 750
    RIPMGEHKLH IGDLKHTILV QPQL 774
    Length:774
    Mass (Da):83,524
    Last modified:March 1, 2001 - v1
    Checksum:i77478F0F28A5DA51
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023034 Genomic DNA. Translation: BAB09906.1.
    CP002688 Genomic DNA. Translation: AED95802.1.
    AY120767 mRNA. Translation: AAM53325.1.
    RefSeqiNP_199747.1. NM_124313.2.
    UniGeneiAt.22961.
    At.28192.
    At.47570.

    Genome annotation databases

    EnsemblPlantsiAT5G49360.1; AT5G49360.1; AT5G49360.
    GeneIDi834996.
    KEGGiath:AT5G49360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023034 Genomic DNA. Translation: BAB09906.1 .
    CP002688 Genomic DNA. Translation: AED95802.1 .
    AY120767 mRNA. Translation: AAM53325.1 .
    RefSeqi NP_199747.1. NM_124313.2.
    UniGenei At.22961.
    At.28192.
    At.47570.

    3D structure databases

    ProteinModelPortali Q9FGY1.
    SMRi Q9FGY1. Positions 59-769.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20242. 2 interactions.

    Protein family/group databases

    CAZyi GH3. Glycoside Hydrolase Family 3.

    Proteomic databases

    PaxDbi Q9FGY1.
    PRIDEi Q9FGY1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G49360.1 ; AT5G49360.1 ; AT5G49360 .
    GeneIDi 834996.
    KEGGi ath:AT5G49360.

    Organism-specific databases

    TAIRi AT5G49360.

    Phylogenomic databases

    eggNOGi COG1472.
    HOGENOMi HOG000031216.
    InParanoidi Q9FGY1.
    OMAi NSDVTET.
    PhylomeDBi Q9FGY1.

    Enzyme and pathway databases

    BioCyci ARA:AT5G49360-MONOMER.
    BRENDAi 3.2.1.37. 399.
    SABIO-RK Q9FGY1.

    Gene expression databases

    Genevestigatori Q9FGY1.

    Family and domain databases

    Gene3Di 3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProi IPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR30620. PTHR30620. 1 hit.
    Pfami PF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00133. GLHYDRLASE3.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
      Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
      Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. "Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
      Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
      Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    6. "Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis."
      Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N.
      Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY SUGAR STARVATION.
    7. "AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase required for pectic arabinan modification in arabidopsis mucilage secretory cells."
      Arsovski A.A., Popma T.M., Haughn G.W., Carpita N.C., McCann M.C., Western T.L.
      Plant Physiol. 150:1219-1234(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiBXL1_ARATH
    AccessioniPrimary (citable) accession number: Q9FGY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Might be processed at the C-terminus.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3