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Q9FGY1

- BXL1_ARATH

UniProt

Q9FGY1 - BXL1_ARATH

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Protein

Beta-D-xylosidase 1

Gene

BXL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pectic arabinan modification in mucilage secretory cells. Acts also as a beta-D-xylosidase during the remodeling of xylans in vascular development.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=1.2 mM for p-nitrophenyl-beta-D-xylopyranoside1 Publication

pH dependencei

Optimum pH is 4.9.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei296 – 2961By similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. seed coat development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G49360-MONOMER.
BRENDAi3.2.1.37. 399.
SABIO-RKQ9FGY1.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-D-xylosidase 1 (EC:3.2.1.-)
Short name:
AtBXL1
Alternative name(s):
Alpha-L-arabinofuranosidase (EC:3.2.1.55)
Gene namesi
Name:BXL1
Synonyms:XYL1
Ordered Locus Names:At5g49360
ORF Names:K7J8.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G49360.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. plant-type cell wall Source: TAIR
  3. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Delayed seeds germination resulting from an altered mucilage composition. No visible growth defects; probably due to partial redundancy with BXL2. Bxl1 and bxl2 double mutants have shortened siliques and curled leaf edges.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 774744Beta-D-xylosidase 1PRO_0000384056Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi658 – 6581N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FGY1.
PRIDEiQ9FGY1.

Expressioni

Tissue specificityi

Expressed in leaves, stems, seedlings, roots, inflorescences, siliques and developing seeds. Expressed in the vasculature of the roots, leaves, flowers and silique. Expressed in tissues undergoing secondary cell wall thickening such as protoxylem, metaxylem, intrafascicular cambium and fibers.3 Publications

Inductioni

By sugar starvation.1 Publication

Gene expression databases

GenevestigatoriQ9FGY1.

Interactioni

Protein-protein interaction databases

BioGridi20242. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9FGY1.
SMRiQ9FGY1. Positions 59-769.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000031216.
InParanoidiQ9FGY1.
OMAiNSDVTET.
PhylomeDBiQ9FGY1.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FGY1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCYNKALLI GNKVVVILVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC
60 70 80 90 100
RANVPIHVRV QDLLGRLTLQ EKIRNLVNNA AAVPRLGIGG YEWWSEALHG
110 120 130 140 150
ISDVGPGAKF GGAFPGATSF PQVITTAASF NQSLWEEIGR VVSDEARAMY
160 170 180 190 200
NGGVAGLTYW SPNVNILRDP RWGRGQETPG EDPIVAAKYA ASYVRGLQGT
210 220 230 240 250
AAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTQQDL EDTYNVPFKS
260 270 280 290 300
CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV
310 320 330 340 350
DVFFNQQHYT STPEEAAARS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND
360 370 380 390 400
INLALANTLT VQMRLGMFDG NLGPYANLGP RDVCTPAHKH LALEAAHQGI
410 420 430 440 450
VLLKNSARSL PLSPRRHRTV AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG
460 470 480 490 500
ISRYARTLHQ AGCAGVACKG NQGFGAAEAA AREADATVLV MGLDQSIEAE
510 520 530 540 550
TRDRTGLLLP GYQQDLVTRV AQASRGPVIL VLMSGGPIDV TFAKNDPRVA
560 570 580 590 600
AIIWAGYPGQ AGGAAIANII FGAANPGGKL PMTWYPQDYV AKVPMTVMAM
610 620 630 640 650
RASGNYPGRT YRFYKGPVVF PFGFGLSYTT FTHSLAKSPL AQLSVSLSNL
660 670 680 690 700
NSANTILNSS SHSIKVSHTN CNSFPKMPLH VEVSNTGEFD GTHTVFVFAE
710 720 730 740 750
PPINGIKGLG VNKQLIAFEK VHVMAGAKQT VQVDVDACKH LGVVDEYGKR
760 770
RIPMGEHKLH IGDLKHTILV QPQL
Length:774
Mass (Da):83,524
Last modified:March 1, 2001 - v1
Checksum:i77478F0F28A5DA51
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023034 Genomic DNA. Translation: BAB09906.1.
CP002688 Genomic DNA. Translation: AED95802.1.
AY120767 mRNA. Translation: AAM53325.1.
RefSeqiNP_199747.1. NM_124313.2.
UniGeneiAt.22961.
At.28192.
At.47570.

Genome annotation databases

EnsemblPlantsiAT5G49360.1; AT5G49360.1; AT5G49360.
GeneIDi834996.
KEGGiath:AT5G49360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023034 Genomic DNA. Translation: BAB09906.1 .
CP002688 Genomic DNA. Translation: AED95802.1 .
AY120767 mRNA. Translation: AAM53325.1 .
RefSeqi NP_199747.1. NM_124313.2.
UniGenei At.22961.
At.28192.
At.47570.

3D structure databases

ProteinModelPortali Q9FGY1.
SMRi Q9FGY1. Positions 59-769.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20242. 2 interactions.

Protein family/group databases

CAZyi GH3. Glycoside Hydrolase Family 3.

Proteomic databases

PaxDbi Q9FGY1.
PRIDEi Q9FGY1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G49360.1 ; AT5G49360.1 ; AT5G49360 .
GeneIDi 834996.
KEGGi ath:AT5G49360.

Organism-specific databases

TAIRi AT5G49360.

Phylogenomic databases

eggNOGi COG1472.
HOGENOMi HOG000031216.
InParanoidi Q9FGY1.
OMAi NSDVTET.
PhylomeDBi Q9FGY1.

Enzyme and pathway databases

BioCyci ARA:AT5G49360-MONOMER.
BRENDAi 3.2.1.37. 399.
SABIO-RK Q9FGY1.

Gene expression databases

Genevestigatori Q9FGY1.

Family and domain databases

Gene3Di 3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProi IPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR30620. PTHR30620. 1 hit.
Pfami PF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view ]
PRINTSi PR00133. GLHYDRLASE3.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
    Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
    Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis."
    Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L.
    Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  6. "Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis."
    Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N.
    Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY SUGAR STARVATION.
  7. "AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase required for pectic arabinan modification in arabidopsis mucilage secretory cells."
    Arsovski A.A., Popma T.M., Haughn G.W., Carpita N.C., McCann M.C., Western T.L.
    Plant Physiol. 150:1219-1234(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiBXL1_ARATH
AccessioniPrimary (citable) accession number: Q9FGY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Might be processed at the C-terminus.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3