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Protein

Beta-D-xylosidase 1

Gene

BXL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pectic arabinan modification in mucilage secretory cells. Acts also as a beta-D-xylosidase during the remodeling of xylans in vascular development.3 Publications

Miscellaneous

Might be processed at the C-terminus.

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=1.2 mM for p-nitrophenyl-beta-D-xylopyranoside1 Publication

    pH dependencei

    Optimum pH is 4.9.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei296By similarity1

    GO - Molecular functioni

    • alpha-L-arabinofuranosidase activity Source: TAIR

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • seed coat development Source: TAIR

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G49360-MONOMER
    BRENDAi3.2.1.37 399
    SABIO-RKiQ9FGY1

    Protein family/group databases

    CAZyiGH3 Glycoside Hydrolase Family 3

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 1 (EC:3.2.1.-)
    Short name:
    AtBXL1
    Alternative name(s):
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Gene namesi
    Name:BXL1
    Synonyms:XYL1
    Ordered Locus Names:At5g49360
    ORF Names:K7J8.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi

    Organism-specific databases

    AraportiAT5G49360
    TAIRilocus:2157994 AT5G49360

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Delayed seeds germination resulting from an altered mucilage composition. No visible growth defects; probably due to partial redundancy with BXL2. Bxl1 and bxl2 double mutants have shortened siliques and curled leaf edges.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 30Sequence analysisAdd BLAST30
    ChainiPRO_000038405631 – 774Beta-D-xylosidase 1Add BLAST744

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi658N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9FGY1
    PRIDEiQ9FGY1

    Expressioni

    Tissue specificityi

    Expressed in leaves, stems, seedlings, roots, inflorescences, siliques and developing seeds. Expressed in the vasculature of the roots, leaves, flowers and silique. Expressed in tissues undergoing secondary cell wall thickening such as protoxylem, metaxylem, intrafascicular cambium and fibers.3 Publications

    Inductioni

    By sugar starvation.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9FGY1 baseline and differential
    GenevisibleiQ9FGY1 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi20242, 3 interactors
    STRINGi3702.AT5G49360.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FGY1
    SMRiQ9FGY1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IESU Eukaryota
    ENOG410JBDH Eukaryota
    COG1472 LUCA
    HOGENOMiHOG000031216
    InParanoidiQ9FGY1
    OMAiAKVPMTI
    OrthoDBiEOG0936046Q
    PhylomeDBiQ9FGY1

    Family and domain databases

    Gene3Di2.60.40.10, 1 hit
    3.20.20.300, 1 hit
    3.40.50.1700, 1 hit
    InterProiView protein in InterPro
    IPR026891 Fn3-like
    IPR002772 Glyco_hydro_3_C
    IPR036881 Glyco_hydro_3_C_sf
    IPR001764 Glyco_hydro_3_N
    IPR036962 Glyco_hydro_3_N_sf
    IPR017853 Glycoside_hydrolase_SF
    IPR013783 Ig-like_fold
    PfamiView protein in Pfam
    PF14310 Fn3-like, 1 hit
    PF00933 Glyco_hydro_3, 1 hit
    PF01915 Glyco_hydro_3_C, 1 hit
    PRINTSiPR00133 GLHYDRLASE3
    SMARTiView protein in SMART
    SM01217 Fn3_like, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit
    SSF52279 SSF52279, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FGY1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCYNKALLI GNKVVVILVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC
    60 70 80 90 100
    RANVPIHVRV QDLLGRLTLQ EKIRNLVNNA AAVPRLGIGG YEWWSEALHG
    110 120 130 140 150
    ISDVGPGAKF GGAFPGATSF PQVITTAASF NQSLWEEIGR VVSDEARAMY
    160 170 180 190 200
    NGGVAGLTYW SPNVNILRDP RWGRGQETPG EDPIVAAKYA ASYVRGLQGT
    210 220 230 240 250
    AAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTQQDL EDTYNVPFKS
    260 270 280 290 300
    CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV
    310 320 330 340 350
    DVFFNQQHYT STPEEAAARS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND
    360 370 380 390 400
    INLALANTLT VQMRLGMFDG NLGPYANLGP RDVCTPAHKH LALEAAHQGI
    410 420 430 440 450
    VLLKNSARSL PLSPRRHRTV AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG
    460 470 480 490 500
    ISRYARTLHQ AGCAGVACKG NQGFGAAEAA AREADATVLV MGLDQSIEAE
    510 520 530 540 550
    TRDRTGLLLP GYQQDLVTRV AQASRGPVIL VLMSGGPIDV TFAKNDPRVA
    560 570 580 590 600
    AIIWAGYPGQ AGGAAIANII FGAANPGGKL PMTWYPQDYV AKVPMTVMAM
    610 620 630 640 650
    RASGNYPGRT YRFYKGPVVF PFGFGLSYTT FTHSLAKSPL AQLSVSLSNL
    660 670 680 690 700
    NSANTILNSS SHSIKVSHTN CNSFPKMPLH VEVSNTGEFD GTHTVFVFAE
    710 720 730 740 750
    PPINGIKGLG VNKQLIAFEK VHVMAGAKQT VQVDVDACKH LGVVDEYGKR
    760 770
    RIPMGEHKLH IGDLKHTILV QPQL
    Length:774
    Mass (Da):83,524
    Last modified:March 1, 2001 - v1
    Checksum:i77478F0F28A5DA51
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB023034 Genomic DNA Translation: BAB09906.1
    CP002688 Genomic DNA Translation: AED95802.1
    AY120767 mRNA Translation: AAM53325.1
    RefSeqiNP_199747.1, NM_124313.3
    UniGeneiAt.22961
    At.28192
    At.47570

    Genome annotation databases

    EnsemblPlantsiAT5G49360.1; AT5G49360.1; AT5G49360
    GeneIDi834996
    GrameneiAT5G49360.1; AT5G49360.1; AT5G49360
    KEGGiath:AT5G49360

    Similar proteinsi

    Entry informationi

    Entry nameiBXL1_ARATH
    AccessioniPrimary (citable) accession number: Q9FGY1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: March 1, 2001
    Last modified: April 25, 2018
    This is version 100 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

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