Q9FGY1 (BXL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-D-xylosidase 1
Short name=AtBXL1
EC=3.2.1.-

Alternative name(s):
Alpha-L-arabinofuranosidase
EC=3.2.1.55

Gene names

Name:	BXL1
Synonyms:	XYL1
Ordered Locus Names:	At5g49360
ORF Names:	K7J8.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pectic arabinan modification in mucilage secretory cells. Acts also as a beta-D-xylosidase during the remodeling of xylans in vascular development. Ref.4 Ref.5 Ref.7
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. Ref.5
Subcellular location	Secreted › extracellular space › extracellular matrix Probable.
Tissue specificity	Expressed in leaves, stems, seedlings, roots, inflorescences, siliques and developing seeds. Expressed in the vasculature of the roots, leaves, flowers and silique. Expressed in tissues undergoing secondary cell wall thickening such as protoxylem, metaxylem, intrafascicular cambium and fibers. Ref.4 Ref.5 Ref.7
Induction	By sugar starvation. Ref.6
Disruption phenotype	Delayed seeds germination resulting from an altered mucilage composition. No visible growth defects; probably due to partial redundancy with BXL2. Bxl1/bxl2 double mutants have shortened siliques and curled leaf edges. Ref.7
Miscellaneous	Might be processed at the C-terminus.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.
Biophysicochemical properties	Kinetic parameters: KM=1.2 mM for p-nitrophenyl-beta-D-xylopyranoside Ref.5 pH dependence: Optimum pH is 4.9. Temperature dependence: Optimum temperature is 55 degrees Celsius.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro seed coat development Inferred from mutant phenotype Ref.7. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR plant-type cell wall Inferred from direct assay PubMed 17526915. Source: TAIR proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 774	744	Beta-D-xylosidase 1		PRO_0000384056
Sites
	Active site	296	1	By similarity
Amino acid modifications
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	658	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FGY1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 77478F0F28A5DA51	FASTA	774	83,524
	10 20 30 40 50 60 MSCYNKALLI GNKVVVILVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC RANVPIHVRV 70 80 90 100 110 120 QDLLGRLTLQ EKIRNLVNNA AAVPRLGIGG YEWWSEALHG ISDVGPGAKF GGAFPGATSF 130 140 150 160 170 180 PQVITTAASF NQSLWEEIGR VVSDEARAMY NGGVAGLTYW SPNVNILRDP RWGRGQETPG 190 200 210 220 230 240 EDPIVAAKYA ASYVRGLQGT AAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTQQDL 250 260 270 280 290 300 EDTYNVPFKS CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV 310 320 330 340 350 360 DVFFNQQHYT STPEEAAARS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND INLALANTLT 370 380 390 400 410 420 VQMRLGMFDG NLGPYANLGP RDVCTPAHKH LALEAAHQGI VLLKNSARSL PLSPRRHRTV 430 440 450 460 470 480 AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG ISRYARTLHQ AGCAGVACKG NQGFGAAEAA 490 500 510 520 530 540 AREADATVLV MGLDQSIEAE TRDRTGLLLP GYQQDLVTRV AQASRGPVIL VLMSGGPIDV 550 560 570 580 590 600 TFAKNDPRVA AIIWAGYPGQ AGGAAIANII FGAANPGGKL PMTWYPQDYV AKVPMTVMAM 610 620 630 640 650 660 RASGNYPGRT YRFYKGPVVF PFGFGLSYTT FTHSLAKSPL AQLSVSLSNL NSANTILNSS 670 680 690 700 710 720 SHSIKVSHTN CNSFPKMPLH VEVSNTGEFD GTHTVFVFAE PPINGIKGLG VNKQLIAFEK 730 740 750 760 770 VHVMAGAKQT VQVDVDACKH LGVVDEYGKR RIPMGEHKLH IGDLKHTILV QPQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development." Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L. Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis." Minic Z., Rihouey C., Do C.T., Lerouge P., Jouanin L. Plant Physiol. 135:867-878(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[6]	"Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis." Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N. Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY SUGAR STARVATION.
[7]	"AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase required for pectic arabinan modification in arabidopsis mucilage secretory cells." Arsovski A.A., Popma T.M., Haughn G.W., Carpita N.C., McCann M.C., Western T.L. Plant Physiol. 150:1219-1234(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023034 Genomic DNA. Translation: BAB09906.1. CP002688 Genomic DNA. Translation: AED95802.1. AY120767 mRNA. Translation: AAM53325.1.
IPI	IPI00528268.
RefSeq	NP_199747.1. NM_124313.2.
UniGene	At.22961. At.28192. At.47570.
3D structure databases
ProteinModelPortal	Q9FGY1.
SMR	Q9FGY1. Positions 93-427, 476-769.
ModBase	Search...
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Proteomic databases
PaxDb	Q9FGY1.
PRIDE	Q9FGY1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G49360.1; AT5G49360.1; AT5G49360.
GeneID	834996.
KEGG	ath:AT5G49360.
Organism-specific databases
TAIR	At5g49360.
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HOG000031216.
InParanoid	Q9FGY1.
OMA	IATPKHI.
PhylomeDB	Q9FGY1.
ProtClustDB	CLSN2686817.
Enzyme and pathway databases
BRENDA	3.2.1.37. 399.
Gene expression databases
ArrayExpress	Q9FGY1.
Genevestigator	Q9FGY1.
Family and domain databases
Gene3D	3.20.20.300. 1 hit. 3.40.50.1700. 1 hit.
InterPro	IPR026891. Fn3-like. IPR026892. Glyco_hydro_3. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR30620. PTHR30620. 1 hit.
Pfam	PF14310. Fn3-like. 1 hit. PF00933. Glyco_hydro_3. 1 hit. PF01915. Glyco_hydro_3_C. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
SUPFAM	SSF52279. Glyco_hydro_3_C. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BXL1_ARATH

Accession

Primary (citable) accession number: Q9FGY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program