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Protein

KDEL-tailed cysteine endopeptidase CEP1

Gene

CEP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses protease activity in vitro (PubMed:25035401). Involved in the final stage of developmental programmed cell death and in intercalation of new cells. Cleaves extensins, thus probably supporting the final cell collapse (PubMed:21632425). During the compatible interaction with the biotrophic powdery mildew fungus Erysiphe cruciferarum, involved in the control of late epidermal cell death that limits growth and susceptibility to the parasite (PubMed:24605116). During anther development, involved in tapetal programmed cell death (PCD), leading to degeneration of tapetal cells and functional pollen formation (PubMed:25035401).3 Publications

Enzyme regulationi

Inhibited by leupeptin and the cysteine protease inhibitor E-64.1 Publication

Kineticsi

  1. KM=13.92 µM for Z-Phe-Arg-NHMec1 Publication
  2. KM=159.62 µM for Z-Arg-NHMec1 Publication
  1. Vmax=235.14 pmol/sec/mg enzyme toward Z-Phe-Arg-NHMec1 Publication
  2. Vmax=537.93 pmol/sec/mg enzyme toward Z-Arg-NHMec1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei150 – 1501PROSITE-ProRule annotation
Active sitei286 – 2861PROSITE-ProRule annotation
Active sitei307 – 3071PROSITE-ProRule annotation

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • anther wall tapetum development Source: TAIR
  • defense response Source: UniProtKB-KW
  • developmental programmed cell death Source: TAIR
  • pollen development Source: TAIR
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT5G50260-MONOMER.

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
KDEL-tailed cysteine endopeptidase CEP1 (EC:3.4.22.-)
Alternative name(s):
Cysteine proteinase CP56
Short name:
AtCP56
Gene namesi
Name:CEP11 Publication
Synonyms:CP561 Publication
Ordered Locus Names:At5g50260Imported
ORF Names:K6A12.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G50260.

Subcellular locationi

  • Endoplasmic reticulum PROSITE-ProRule annotation1 Publication
  • Vacuole 1 Publication

  • Note: During anther development, detected as a proenzyme in precursor protease vesicles in tapetum cells and then transported to the vacuole and transformed into the mature enzyme before rupture of the vacuole.1 Publication

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB-SubCell
  • extracellular space Source: GO_Central
  • lysosome Source: GO_Central
  • plant-type cell wall Source: TAIR
  • plant-type vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Vacuole

Pathology & Biotechi

Disruption phenotypei

Reduced male fertility due to impaired pollen development and abnormal pollen exine (PubMed:25035401). Enhanced susceptibility to powdery mildew caused by the biotrophic ascomycete Erysiphe cruciferarum (PubMed:24605116).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 125105Activation peptideCuratedPRO_0000436321Add
BLAST
Chaini126 – 361236KDEL-tailed cysteine endopeptidase CEP1PRO_0000403789Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi147 ↔ 189By similarity
Disulfide bondi181 ↔ 222By similarity
Disulfide bondi280 ↔ 332By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ9FGR9.
PRIDEiQ9FGR9.

PTM databases

iPTMnetiQ9FGR9.

Expressioni

Tissue specificityi

Expressed in roots, stems, flowers, buds and green siliques. Found within columella, lateral root cap cells, and in the endodermis, the cortex and the epidermis during lateral root formation. Expressed in the abscission zones of the flower organs.1 Publication

Developmental stagei

Expressed when organs wither and separate from the fruit or the green silique. Expressed in unpollinated, degrading ovules (PubMed:21632425). Expressed specifically in the tapetum from stages 5 to 11 of anther development (PubMed:25035401).2 Publications

Gene expression databases

GenevisibleiQ9FGR9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G50260.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FGR9.
SMRiQ9FGR9. Positions 31-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi358 – 3614Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230773.
InParanoidiQ9FGR9.
KOiK16292.
OMAiFMYANVN.
PhylomeDBiQ9FGR9.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FGR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFIVLALC MLMVLETTKG LDFHNKDVES ENSLWELYER WRSHHTVARS
60 70 80 90 100
LEEKAKRFNV FKHNVKHIHE TNKKDKSYKL KLNKFGDMTS EEFRRTYAGS
110 120 130 140 150
NIKHHRMFQG EKKATKSFMY ANVNTLPTSV DWRKNGAVTP VKNQGQCGSC
160 170 180 190 200
WAFSTVVAVE GINQIRTKKL TSLSEQELVD CDTNQNQGCN GGLMDLAFEF
210 220 230 240 250
IKEKGGLTSE LVYPYKASDE TCDTNKENAP VVSIDGHEDV PKNSEDDLMK
260 270 280 290 300
AVANQPVSVA IDAGGSDFQF YSEGVFTGRC GTELNHGVAV VGYGTTIDGT
310 320 330 340 350
KYWIVKNSWG EEWGEKGYIR MQRGIRHKEG LCGIAMEASY PLKNSNTNPS
360
RLSLDSLKDE L
Length:361
Mass (Da):40,708
Last modified:March 1, 2001 - v1
Checksum:iA622234BC3E7367A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367092 mRNA. Translation: ADO14465.1.
AB024031 Genomic DNA. Translation: BAB09397.1.
CP002688 Genomic DNA. Translation: AED95919.1.
AY091087 mRNA. Translation: AAM13907.1.
RefSeqiNP_568722.1. NM_124405.2.
UniGeneiAt.7918.

Genome annotation databases

EnsemblPlantsiAT5G50260.1; AT5G50260.1; AT5G50260.
GeneIDi835091.
GrameneiAT5G50260.1; AT5G50260.1; AT5G50260.
KEGGiath:AT5G50260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367092 mRNA. Translation: ADO14465.1.
AB024031 Genomic DNA. Translation: BAB09397.1.
CP002688 Genomic DNA. Translation: AED95919.1.
AY091087 mRNA. Translation: AAM13907.1.
RefSeqiNP_568722.1. NM_124405.2.
UniGeneiAt.7918.

3D structure databases

ProteinModelPortaliQ9FGR9.
SMRiQ9FGR9. Positions 31-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G50260.1.

Protein family/group databases

MEROPSiI29.003.

PTM databases

iPTMnetiQ9FGR9.

Proteomic databases

PaxDbiQ9FGR9.
PRIDEiQ9FGR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G50260.1; AT5G50260.1; AT5G50260.
GeneIDi835091.
GrameneiAT5G50260.1; AT5G50260.1; AT5G50260.
KEGGiath:AT5G50260.

Organism-specific databases

TAIRiAT5G50260.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230773.
InParanoidiQ9FGR9.
KOiK16292.
OMAiFMYANVN.
PhylomeDBiQ9FGR9.

Enzyme and pathway databases

BioCyciARA:AT5G50260-MONOMER.

Miscellaneous databases

PROiQ9FGR9.

Gene expression databases

GenevisibleiQ9FGR9. AT.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Function analysis of an Arabidopsis cysteine proteinase gene AtCP56 and the promoter ProCP56 in anther development."
    Lv X., Li F., Lu H.
    Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "KDEL-tailed cysteine endopeptidases involved in programmed cell death, intercalation of new cells, and dismantling of extensin scaffolds."
    Helm M., Schmid M., Hierl G., Terneus K., Tan L., Lottspeich F., Kieliszewski M.J., Gietl C.
    Am. J. Bot. 95:1049-1062(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Endoplasmic reticulum KDEL-tailed cysteine endopeptidase 1 of Arabidopsis (AtCEP1) is involved in pathogen defense."
    Hoewing T., Huesmann C., Hoefle C., Nagel M.K., Isono E., Hueckelhoven R., Gietl C.
    Front. Plant Sci. 5:58-58(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  7. "The cysteine protease CEP1, a key executor involved in tapetal programmed cell death, regulates pollen development in Arabidopsis."
    Zhang D., Liu D., Lv X., Wang Y., Xun Z., Liu Z., Li F., Lu H.
    Plant Cell 26:2939-2961(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCEP1_ARATH
AccessioniPrimary (citable) accession number: Q9FGR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.