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Protein

KDEL-tailed cysteine endopeptidase CEP1

Gene

CEP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the final stage of developmental programmed cell death and in intercalation of new cells. Cleaves extensins, thus probably supporting the final cell collapse.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei150 – 1501PROSITE-ProRule annotation
Active sitei286 – 2861PROSITE-ProRule annotation
Active sitei307 – 3071PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: TAIR

GO - Biological processi

  1. anther wall tapetum development Source: TAIR
  2. developmental programmed cell death Source: TAIR
  3. pollen development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciARA:AT5G50260-MONOMER.
ReactomeiREACT_319570. MHC class II antigen presentation.
REACT_352268. Endosomal/Vacuolar pathway.

Protein family/group databases

MEROPSiC01.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
KDEL-tailed cysteine endopeptidase CEP1 (EC:3.4.22.-)
Alternative name(s):
Cysteine proteinase CP56
Short name:
AtCP56
Gene namesi
Name:CEP11 Publication
Synonyms:CP561 Publication
Ordered Locus Names:At5g50260Imported
ORF Names:K6A12.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G50260.

Subcellular locationi

  1. Endoplasmic reticulum PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
  2. plant-type cell wall Source: TAIR
  3. plant-type vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 361341KDEL-tailed cysteine endopeptidase CEP1PRO_0000403789Add
BLAST

Proteomic databases

PaxDbiQ9FGR9.
PRIDEiQ9FGR9.

Expressioni

Tissue specificityi

Expressed in roots, stems, flowers, buds and green siliques. Found within columella, lateral root cap cells, and in the endodermis, the cortex and the epidermis during lateral root formation. Expressed in the abscission zones of the flower organs.1 Publication

Developmental stagei

Expressed when organs wither and separate from the fruit or the green silique. Expressed in unpollinated, degrading ovules.1 Publication

Gene expression databases

GenevestigatoriQ9FGR9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G50260.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FGR9.
SMRiQ9FGR9. Positions 31-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi358 – 3614Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4870.
HOGENOMiHOG000230773.
InParanoidiQ9FGR9.
KOiK16292.
OMAiPENNENA.
PhylomeDBiQ9FGR9.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FGR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFIVLALC MLMVLETTKG LDFHNKDVES ENSLWELYER WRSHHTVARS
60 70 80 90 100
LEEKAKRFNV FKHNVKHIHE TNKKDKSYKL KLNKFGDMTS EEFRRTYAGS
110 120 130 140 150
NIKHHRMFQG EKKATKSFMY ANVNTLPTSV DWRKNGAVTP VKNQGQCGSC
160 170 180 190 200
WAFSTVVAVE GINQIRTKKL TSLSEQELVD CDTNQNQGCN GGLMDLAFEF
210 220 230 240 250
IKEKGGLTSE LVYPYKASDE TCDTNKENAP VVSIDGHEDV PKNSEDDLMK
260 270 280 290 300
AVANQPVSVA IDAGGSDFQF YSEGVFTGRC GTELNHGVAV VGYGTTIDGT
310 320 330 340 350
KYWIVKNSWG EEWGEKGYIR MQRGIRHKEG LCGIAMEASY PLKNSNTNPS
360
RLSLDSLKDE L
Length:361
Mass (Da):40,708
Last modified:March 1, 2001 - v1
Checksum:iA622234BC3E7367A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367092 mRNA. Translation: ADO14465.1.
AB024031 Genomic DNA. Translation: BAB09397.1.
CP002688 Genomic DNA. Translation: AED95919.1.
AY091087 mRNA. Translation: AAM13907.1.
RefSeqiNP_568722.1. NM_124405.2.
UniGeneiAt.7918.

Genome annotation databases

EnsemblPlantsiAT5G50260.1; AT5G50260.1; AT5G50260.
GeneIDi835091.
KEGGiath:AT5G50260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367092 mRNA. Translation: ADO14465.1.
AB024031 Genomic DNA. Translation: BAB09397.1.
CP002688 Genomic DNA. Translation: AED95919.1.
AY091087 mRNA. Translation: AAM13907.1.
RefSeqiNP_568722.1. NM_124405.2.
UniGeneiAt.7918.

3D structure databases

ProteinModelPortaliQ9FGR9.
SMRiQ9FGR9. Positions 31-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G50260.1-P.

Protein family/group databases

MEROPSiC01.A03.

Proteomic databases

PaxDbiQ9FGR9.
PRIDEiQ9FGR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G50260.1; AT5G50260.1; AT5G50260.
GeneIDi835091.
KEGGiath:AT5G50260.

Organism-specific databases

TAIRiAT5G50260.

Phylogenomic databases

eggNOGiCOG4870.
HOGENOMiHOG000230773.
InParanoidiQ9FGR9.
KOiK16292.
OMAiPENNENA.
PhylomeDBiQ9FGR9.

Enzyme and pathway databases

BioCyciARA:AT5G50260-MONOMER.
ReactomeiREACT_319570. MHC class II antigen presentation.
REACT_352268. Endosomal/Vacuolar pathway.

Miscellaneous databases

PROiQ9FGR9.

Gene expression databases

GenevestigatoriQ9FGR9.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Function analysis of an Arabidopsis cysteine proteinase gene AtCP56 and the promoter ProCP56 in anther development."
    Lv X., Li F., Lu H.
    Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "KDEL-tailed cysteine endopeptidases involved in programmed cell death, intercalation of new cells, and dismantling of extensin scaffolds."
    Helm M., Schmid M., Hierl G., Terneus K., Tan L., Lottspeich F., Kieliszewski M.J., Gietl C.
    Am. J. Bot. 95:1049-1062(2008)
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCEP1_ARATH
AccessioniPrimary (citable) accession number: Q9FGR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.