Q9FGR9 (CEP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KDEL-tailed cysteine endopeptidase CEP1
EC=3.4.22.-

Alternative name(s):
Cysteine proteinase CP56
Short name=AtCP56

Gene names

Name:	CEP1
Synonyms:	CP56
Ordered Locus Names:	At5g50260
ORF Names:	K6A12.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the final stage of developmental programmed cell death and in intercalation of new cells. Cleaves extensins, thus probably supporing the final cell collapse By similarity. Ref.5
Subcellular location	Endoplasmic reticulum Probable.
Tissue specificity	Expressed in roots, stems, flowers, buds and green siliques. Found within columella, lateral root cap cells, and in the endodermis, the cortex and the epidermis during lateral root formation. Expressed in the abscission zones of the flower organs. Ref.5
Developmental stage	Expressed when organs wither and separate from the fruit or the green silique. Expressed in unpollinated, degrading ovules. Ref.5
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 361	341	KDEL-tailed cysteine endopeptidase CEP1		PRO_0000403789
Regions
	Motif	358 – 361	4	Prevents secretion from ER Potential
Sites
	Active site	150	1	By similarity
	Active site	286	1	By similarity
	Active site	307	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FGR9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A622234BC3E7367A	FASTA	361	40,708
	10 20 30 40 50 60 MKRFIVLALC MLMVLETTKG LDFHNKDVES ENSLWELYER WRSHHTVARS LEEKAKRFNV 70 80 90 100 110 120 FKHNVKHIHE TNKKDKSYKL KLNKFGDMTS EEFRRTYAGS NIKHHRMFQG EKKATKSFMY 130 140 150 160 170 180 ANVNTLPTSV DWRKNGAVTP VKNQGQCGSC WAFSTVVAVE GINQIRTKKL TSLSEQELVD 190 200 210 220 230 240 CDTNQNQGCN GGLMDLAFEF IKEKGGLTSE LVYPYKASDE TCDTNKENAP VVSIDGHEDV 250 260 270 280 290 300 PKNSEDDLMK AVANQPVSVA IDAGGSDFQF YSEGVFTGRC GTELNHGVAV VGYGTTIDGT 310 320 330 340 350 360 KYWIVKNSWG EEWGEKGYIR MQRGIRHKEG LCGIAMEASY PLKNSNTNPS RLSLDSLKDE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Function analysis of an Arabidopsis cysteine proteinase gene AtCP56 and the promoter ProCP56 in anther development." Lv X., Li F., Lu H. Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"KDEL-tailed cysteine endopeptidases involved in programmed cell death, intercalation of new cells, and dismantling of extensin scaffolds." Helm M., Schmid M., Hierl G., Terneus K., Tan L., Lottspeich F., Kieliszewski M.J., Gietl C. Am. J. Bot. 95:1049-1062(2008) Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HM367092 mRNA. Translation: ADO14465.1. AB024031 Genomic DNA. Translation: BAB09397.1. CP002688 Genomic DNA. Translation: AED95919.1. AY091087 mRNA. Translation: AAM13907.1.
IPI	IPI00516991.
RefSeq	NP_568722.1. NM_124405.2.
UniGene	At.7918.
3D structure databases
HSSP	HSSP built from PDB template 1S4V based on UniProtKB O65039.
ProteinModelPortal	Q9FGR9.
SMR	Q9FGR9. Positions 31-350.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G50260.1-P.
Protein family/group databases
MEROPS	I29.003.
Proteomic databases
PaxDb	Q9FGR9.
PRIDE	Q9FGR9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G50260.1; AT5G50260.1; AT5G50260.
GeneID	835091.
KEGG	ath:AT5G50260.
Organism-specific databases
TAIR	At5g50260.
Phylogenomic databases
eggNOG	COG4870.
HOGENOM	HOG000230773.
InParanoid	Q9FGR9.
KO	K16292.
OMA	WHSKKYH.
PhylomeDB	Q9FGR9.
ProtClustDB	CLSN2689970.
Gene expression databases
Genevestigator	Q9FGR9.
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEP1_ARATH

Accession

Primary (citable) accession number: Q9FGR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program