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Protein

KDEL-tailed cysteine endopeptidase CEP1

Gene

CEP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses protease activity in vitro (PubMed:25035401). Involved in the final stage of developmental programmed cell death and in intercalation of new cells. Cleaves extensins, thus probably supporting the final cell collapse (PubMed:21632425). During the compatible interaction with the biotrophic powdery mildew fungus Erysiphe cruciferarum, involved in the control of late epidermal cell death that limits growth and susceptibility to the parasite (PubMed:24605116). During anther development, involved in tapetal programmed cell death (PCD), leading to degeneration of tapetal cells and functional pollen formation (PubMed:25035401).3 Publications

Enzyme regulationi

Inhibited by leupeptin and the cysteine protease inhibitor E-64.1 Publication

Kineticsi

  1. KM=13.92 µM for Z-Phe-Arg-NHMec1 Publication
  2. KM=159.62 µM for Z-Arg-NHMec1 Publication
  1. Vmax=235.14 pmol/sec/mg enzyme toward Z-Phe-Arg-NHMec1 Publication
  2. Vmax=537.93 pmol/sec/mg enzyme toward Z-Arg-NHMec1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei150PROSITE-ProRule annotation1
Active sitei286PROSITE-ProRule annotation1
Active sitei307PROSITE-ProRule annotation1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • anther wall tapetum development Source: TAIR
  • defense response to fungus Source: UniProtKB
  • plant-type hypersensitive response Source: TAIR
  • pollen development Source: TAIR
  • programmed cell death involved in cell development Source: TAIR
  • proteolysis involved in cellular protein catabolic process Source: GO_Central

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processPlant defense

Protein family/group databases

MEROPSiI29.003

Names & Taxonomyi

Protein namesi
Recommended name:
KDEL-tailed cysteine endopeptidase CEP1 (EC:3.4.22.-)
Alternative name(s):
Cysteine proteinase CP56
Short name:
AtCP56
Gene namesi
Name:CEP11 Publication
Synonyms:CP561 Publication
Ordered Locus Names:At5g50260Imported
ORF Names:K6A12.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G50260
TAIRilocus:2157712 AT5G50260

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Vacuole

Pathology & Biotechi

Disruption phenotypei

Reduced male fertility due to impaired pollen development and abnormal pollen exine (PubMed:25035401). Enhanced susceptibility to powdery mildew caused by the biotrophic ascomycete Erysiphe cruciferarum (PubMed:24605116).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000043632121 – 125Activation peptideCuratedAdd BLAST105
ChainiPRO_0000403789126 – 361KDEL-tailed cysteine endopeptidase CEP1Add BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi147 ↔ 189By similarity
Disulfide bondi181 ↔ 222By similarity
Disulfide bondi280 ↔ 332By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ9FGR9

PTM databases

iPTMnetiQ9FGR9

Expressioni

Tissue specificityi

Expressed in roots, stems, flowers, buds and green siliques. Found within columella, lateral root cap cells, and in the endodermis, the cortex and the epidermis during lateral root formation. Expressed in the abscission zones of the flower organs.1 Publication

Developmental stagei

Expressed when organs wither and separate from the fruit or the green silique. Expressed in unpollinated, degrading ovules (PubMed:21632425). Expressed specifically in the tapetum from stages 5 to 11 of anther development (PubMed:25035401).2 Publications

Gene expression databases

ExpressionAtlasiQ9FGR9 baseline and differential
GenevisibleiQ9FGR9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G50260.1

Structurei

3D structure databases

ProteinModelPortaliQ9FGR9
SMRiQ9FGR9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi358 – 361Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
HOGENOMiHOG000230773
InParanoidiQ9FGR9
KOiK16292
OMAiQHYRKSG
OrthoDBiEOG09360FGY
PhylomeDBiQ9FGR9

Family and domain databases

InterProiView protein in InterPro
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FGR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFIVLALC MLMVLETTKG LDFHNKDVES ENSLWELYER WRSHHTVARS
60 70 80 90 100
LEEKAKRFNV FKHNVKHIHE TNKKDKSYKL KLNKFGDMTS EEFRRTYAGS
110 120 130 140 150
NIKHHRMFQG EKKATKSFMY ANVNTLPTSV DWRKNGAVTP VKNQGQCGSC
160 170 180 190 200
WAFSTVVAVE GINQIRTKKL TSLSEQELVD CDTNQNQGCN GGLMDLAFEF
210 220 230 240 250
IKEKGGLTSE LVYPYKASDE TCDTNKENAP VVSIDGHEDV PKNSEDDLMK
260 270 280 290 300
AVANQPVSVA IDAGGSDFQF YSEGVFTGRC GTELNHGVAV VGYGTTIDGT
310 320 330 340 350
KYWIVKNSWG EEWGEKGYIR MQRGIRHKEG LCGIAMEASY PLKNSNTNPS
360
RLSLDSLKDE L
Length:361
Mass (Da):40,708
Last modified:March 1, 2001 - v1
Checksum:iA622234BC3E7367A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367092 mRNA Translation: ADO14465.1
AB024031 Genomic DNA Translation: BAB09397.1
CP002688 Genomic DNA Translation: AED95919.1
AY091087 mRNA Translation: AAM13907.1
RefSeqiNP_568722.1, NM_124405.3
UniGeneiAt.7918

Genome annotation databases

EnsemblPlantsiAT5G50260.1; AT5G50260.1; AT5G50260
GeneIDi835091
GrameneiAT5G50260.1; AT5G50260.1; AT5G50260
KEGGiath:AT5G50260

Similar proteinsi

Entry informationi

Entry nameiCEP1_ARATH
AccessioniPrimary (citable) accession number: Q9FGR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health