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Protein

Abscisic acid receptor PYL8

Gene

PYL8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) in an ABA-independent manner but more efficiently when activated by ABA. Confers enhanced sensitivity to ABA (PubMed:19407143, PubMed:19624469, PubMed:19769575, PubMed:19889877, PubMed:23844015, PubMed:21658606). Can be activated by both (-)-ABA and (+)-ABA (PubMed:23844015).6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ABABy similarity1
Sitei62Involved in ABA bindingBy similarity1
Sitei88Involved in interactions with PP2CsBy similarity1
Sitei108Involved in ABA bindingBy similarity1
Binding sitei141ABABy similarity1
Sitei152Involved in interactions with PP2CsBy similarity1
Sitei160Involved in ABA bindingBy similarity1
Sitei163Involved in ABA bindingBy similarity1

GO - Molecular functioni

  • abscisic acid binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • protein phosphatase inhibitor activity Source: UniProtKB
  • receptor activity Source: UniProtKB

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB
  • positive regulation of abscisic acid-activated signaling pathway Source: TAIR
  • regulation of protein serine/threonine phosphatase activity Source: GO_Central
  • response to abscisic acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor, Receptor

Keywords - Biological processi

Abscisic acid signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Abscisic acid receptor PYL8
Alternative name(s):
ABI1-binding protein 1
PYR1-like protein 8
Regulatory components of ABA receptor 3
Gene namesi
Name:PYL8
Synonyms:ABIP1, RCAR3
Ordered Locus Names:At5g53160
ORF Names:MFH8.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G53160.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003917431 – 188Abscisic acid receptor PYL8Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 157ReversibleBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9FGM1.
PRIDEiQ9FGM1.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FGM1. baseline and differential.
GenevisibleiQ9FGM1. AT.

Interactioni

Subunit structurei

Monomer (PubMed:21658606). Homodimer. Binds ABA on one subunit only (By similarity). interacts with ABI1 and HAB1, and possibly with other PP2Cs (PubMed:19624469, PubMed:19769575, PubMed:19874541). Binds to CARs protein in an ABA-independent manner, both at the plasma membrane and in the nucleus. Interacts directly with CAR1 and CAR4 (PubMed:25465408).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HAB1Q9CAJ03EBI-2429535,EBI-2309302

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20642. 19 interactors.
IntActiQ9FGM1. 4 interactors.
MINTiMINT-8299474.
STRINGi3702.AT5G53160.2.

Structurei

3D structure databases

ProteinModelPortaliQ9FGM1.
SMRiQ9FGM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 176START-likeAdd BLAST152
Regioni89 – 94ABA bindingBy similarity6
Regioni116 – 122ABA bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi85 – 89Gate loopBy similarity5
Motifi115 – 117Latch loopBy similarity3

Domaini

Upon interaction with ABA, the 'latch' and 'gate' loops change in conformation leading to a tight dimerization and the creation a surface that enables the receptor to dock into and inhibit the PP2C active site.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IIK9. Eukaryota.
ENOG4111HU5. LUCA.
HOGENOMiHOG000238422.
InParanoidiQ9FGM1.
KOiK14496.
OMAiHSEMING.
OrthoDBiEOG09360OHL.
PhylomeDBiQ9FGM1.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR019587. Polyketide_cyclase/dehydratase.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF10604. Polyketide_cyc2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FGM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANGIENLT NPNQEREFIR RHHKHELVDN QCSSTLVKHI NAPVHIVWSL
60 70 80 90 100
VRRFDQPQKY KPFISRCVVK GNMEIGTVRE VDVKSGLPAT RSTERLELLD
110 120 130 140 150
DNEHILSIRI VGGDHRLKNY SSIISLHPET IEGRIGTLVI ESFVVDVPEG
160 170 180
NTKDETCYFV EALIKCNLKS LADISERLAV QDTTESRV
Length:188
Mass (Da):21,397
Last modified:March 1, 2001 - v1
Checksum:iD3436ADAC890AEED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6I → F in BAF00266 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025622 Genomic DNA. Translation: BAB08419.1.
CP002688 Genomic DNA. Translation: AED96316.1.
BT003112 mRNA. Translation: AAO24544.1.
AK228324 mRNA. Translation: BAF00266.1.
RefSeqiNP_200128.1. NM_124695.4. [Q9FGM1-1]
UniGeneiAt.24684.
At.72721.

Genome annotation databases

EnsemblPlantsiAT5G53160.2; AT5G53160.2; AT5G53160. [Q9FGM1-1]
GeneIDi835397.
GrameneiAT5G53160.2; AT5G53160.2; AT5G53160.
KEGGiath:AT5G53160.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025622 Genomic DNA. Translation: BAB08419.1.
CP002688 Genomic DNA. Translation: AED96316.1.
BT003112 mRNA. Translation: AAO24544.1.
AK228324 mRNA. Translation: BAF00266.1.
RefSeqiNP_200128.1. NM_124695.4. [Q9FGM1-1]
UniGeneiAt.24684.
At.72721.

3D structure databases

ProteinModelPortaliQ9FGM1.
SMRiQ9FGM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20642. 19 interactors.
IntActiQ9FGM1. 4 interactors.
MINTiMINT-8299474.
STRINGi3702.AT5G53160.2.

Proteomic databases

PaxDbiQ9FGM1.
PRIDEiQ9FGM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G53160.2; AT5G53160.2; AT5G53160. [Q9FGM1-1]
GeneIDi835397.
GrameneiAT5G53160.2; AT5G53160.2; AT5G53160.
KEGGiath:AT5G53160.

Organism-specific databases

TAIRiAT5G53160.

Phylogenomic databases

eggNOGiENOG410IIK9. Eukaryota.
ENOG4111HU5. LUCA.
HOGENOMiHOG000238422.
InParanoidiQ9FGM1.
KOiK14496.
OMAiHSEMING.
OrthoDBiEOG09360OHL.
PhylomeDBiQ9FGM1.

Miscellaneous databases

PROiQ9FGM1.

Gene expression databases

ExpressionAtlasiQ9FGM1. baseline and differential.
GenevisibleiQ9FGM1. AT.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR019587. Polyketide_cyclase/dehydratase.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF10604. Polyketide_cyc2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYL8_ARATH
AccessioniPrimary (citable) accession number: Q9FGM1
Secondary accession number(s): Q0WRI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.