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Protein

Abscisic acid receptor PYL8

Gene

PYL8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) in an ABA-independent manner but more efficiently when activated by ABA. Confers enhanced sensitivity to ABA (PubMed:19407143, PubMed:19624469, PubMed:19769575, PubMed:19889877, PubMed:23844015, PubMed:21658606). Can be activated by both (-)-ABA and (+)-ABA (PubMed:23844015). Mediates crosstalk between ABA and auxin signaling to regulate lateral root growth. Required for lateral root growth suppression by ABA. In response to auxin, promotes lateral root growth by enhancing MYB77-dependent transcription of the auxin-responsive gene IAA19. Enhances the abilities of MYB44 and MYB73 to activate IAA19 gene (PubMed:24894996).7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ABABy similarity1
Sitei62Involved in ABA bindingBy similarity1
Sitei88Involved in interactions with PP2CsBy similarity1
Sitei108Involved in ABA bindingBy similarity1
Binding sitei141ABABy similarity1
Sitei152Involved in interactions with PP2CsBy similarity1
Sitei160Involved in ABA bindingBy similarity1
Sitei163Involved in ABA bindingBy similarity1

GO - Molecular functioni

  • abscisic acid binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • protein phosphatase inhibitor activity Source: UniProtKB
  • signaling receptor activity Source: UniProtKB

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB
  • positive regulation of abscisic acid-activated signaling pathway Source: TAIR
  • response to abscisic acid Source: TAIR

Keywordsi

Molecular functionProtein phosphatase inhibitor, Receptor
Biological processAbscisic acid signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Abscisic acid receptor PYL8
Alternative name(s):
ABI1-binding protein 1
PYR1-like protein 8
Regulatory components of ABA receptor 3
Gene namesi
Name:PYL8
Synonyms:ABIP1, RCAR3
Ordered Locus Names:At5g53160
ORF Names:MFH8.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G53160
TAIRilocus:2163731 AT5G53160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant seedlings exhibit enhanced sensitivity of lateral root inhibition by abscisic acid (ABA), and reduced suppression of primary root growth by ABA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003917431 – 188Abscisic acid receptor PYL8Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 157ReversibleBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9FGM1
PRIDEiQ9FGM1

Expressioni

Inductioni

Down-regulated by abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiQ9FGM1 baseline and differential
GenevisibleiQ9FGM1 AT

Interactioni

Subunit structurei

Monomer (PubMed:21658606). Homodimer. Binds ABA on one subunit only (By similarity). interacts with ABI1 and HAB1, and possibly with other PP2Cs (PubMed:19624469, PubMed:19769575, PubMed:19874541). Binds to CARs protein in an ABA-independent manner, both at the plasma membrane and in the nucleus. Interacts directly with CAR1 and CAR4 (PubMed:25465408). Interacts with MYB44, MYB73 and MYB77 in an ABA-independent manner (PubMed:24894996).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HAB1Q9CAJ03EBI-2429535,EBI-2309302

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20642, 19 interactors
IntActiQ9FGM1, 4 interactors
MINTiQ9FGM1
STRINGi3702.AT5G53160.2

Structurei

3D structure databases

ProteinModelPortaliQ9FGM1
SMRiQ9FGM1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 176START-likeAdd BLAST152
Regioni89 – 94ABA bindingBy similarity6
Regioni116 – 122ABA bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi85 – 89Gate loopBy similarity5
Motifi115 – 117Latch loopBy similarity3

Domaini

Upon interaction with ABA, the 'latch' and 'gate' loops change in conformation leading to a tight dimerization and the creation a surface that enables the receptor to dock into and inhibit the PP2C active site.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IIK9 Eukaryota
ENOG4111HU5 LUCA
HOGENOMiHOG000238422
InParanoidiQ9FGM1
KOiK14496
OMAiRTEPINC
OrthoDBiEOG09360OHL
PhylomeDBiQ9FGM1

Family and domain databases

Gene3Di3.30.530.20, 1 hit
InterProiView protein in InterPro
IPR019587 Polyketide_cyclase/dehydratase
IPR023393 START-like_dom_sf
PfamiView protein in Pfam
PF10604 Polyketide_cyc2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FGM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANGIENLT NPNQEREFIR RHHKHELVDN QCSSTLVKHI NAPVHIVWSL
60 70 80 90 100
VRRFDQPQKY KPFISRCVVK GNMEIGTVRE VDVKSGLPAT RSTERLELLD
110 120 130 140 150
DNEHILSIRI VGGDHRLKNY SSIISLHPET IEGRIGTLVI ESFVVDVPEG
160 170 180
NTKDETCYFV EALIKCNLKS LADISERLAV QDTTESRV
Length:188
Mass (Da):21,397
Last modified:March 1, 2001 - v1
Checksum:iD3436ADAC890AEED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6I → F in BAF00266 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025622 Genomic DNA Translation: BAB08419.1
CP002688 Genomic DNA Translation: AED96316.1
BT003112 mRNA Translation: AAO24544.1
AK228324 mRNA Translation: BAF00266.1
RefSeqiNP_200128.1, NM_124695.4 [Q9FGM1-1]
UniGeneiAt.24684
At.72721

Genome annotation databases

EnsemblPlantsiAT5G53160.2; AT5G53160.2; AT5G53160 [Q9FGM1-1]
GeneIDi835397
GrameneiAT5G53160.2; AT5G53160.2; AT5G53160 [Q9FGM1-1]
KEGGiath:AT5G53160

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPYL8_ARATH
AccessioniPrimary (citable) accession number: Q9FGM1
Secondary accession number(s): Q0WRI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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