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Protein

Aquaporin TIP2-3

Gene

TIP2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports methylammonium or ammonium in yeast cells, preferentially at high medium pH. May participate in vacuolar compartmentation and detoxification of ammonium.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Protein family/group databases

TCDBi1.A.8.10.4. the major intrinsic protein (mip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin TIP2-3
Alternative name(s):
Tonoplast intrinsic protein 2-3
Short name:
AtTIP2;3
Cleaved into the following chain:
Gene namesi
Name:TIP2-3
Ordered Locus Names:At5g47450
ORF Names:MNJ7.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G47450.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 45Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini46 – 54VacuolarSequence analysis9
Transmembranei55 – 75Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini76 – 101CytoplasmicSequence analysisAdd BLAST26
Transmembranei102 – 122Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini123 – 134VacuolarSequence analysisAdd BLAST12
Transmembranei135 – 155Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini156 – 168CytoplasmicSequence analysisAdd BLAST13
Transmembranei169 – 189Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini190 – 217VacuolarSequence analysisAdd BLAST28
Transmembranei218 – 238Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini239 – 250CytoplasmicSequence analysisAdd BLAST12

GO - Cellular componenti

  • central vacuole Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000640131 – 250Aquaporin TIP2-3Add BLAST250
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004257612 – 250Aquaporin TIP2-3, N-terminally processedAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6,N6-dimethyllysineBy similarity1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiQ9FGL2.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By ammonium nitrate in roots. Expressed in roots with a circadian rhythm showing an increase after onset of light, a peak approximately at midday and a decline to lowest levels before offset of light.1 Publication

Gene expression databases

GenevisibleiQ9FGL2. AT.

Interactioni

Subunit structurei

Interacts with cucumber mosaic virus (CMV) Protein 1a.1 Publication

Protein-protein interaction databases

BioGridi20042. 2 interactors.
STRINGi3702.AT5G47450.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FGL2.
SMRiQ9FGL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi83 – 85NPA 13
Motifi197 – 199NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiQ9FGL2.
KOiK09873.
OMAiRSTINGQ.
OrthoDBiEOG09360J95.
PhylomeDBiQ9FGL2.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FGL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKIEVGSVG DSFSVSSLKA YLSEFIATLL FVFAGVGSAV AFAKLTSDGA
60 70 80 90 100
LDPAGLVAIA IAHAFALFVG VSIAANISGG HLNPAVTLGL AIGGNITLIT
110 120 130 140 150
GFFYWIAQCL GSIVACLLLV FVTNGKSVPT HGVSAGLGAV EGVVMEIVVT
160 170 180 190 200
FALVYTVYAT AADPKKGSLG TIAPIAIGFI VGANILAAGP FSGGSMNPAR
210 220 230 240 250
SFGPAVVSGD LSQIWIYWVG PLVGGALAGL IYGDVFIGSY EAVETREIRV
Length:250
Mass (Da):25,245
Last modified:March 1, 2001 - v1
Checksum:i8946CB520D42603C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141E → G in BAF01204 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025628 Genomic DNA. Translation: BAB09071.1.
CP002688 Genomic DNA. Translation: AED95514.1.
BT011212 mRNA. Translation: AAR92248.1.
BT011663 mRNA. Translation: AAS47669.1.
AK229341 mRNA. Translation: BAF01204.1.
RefSeqiNP_199556.1. NM_124117.3.
UniGeneiAt.43789.

Genome annotation databases

EnsemblPlantsiAT5G47450.1; AT5G47450.1; AT5G47450.
GeneIDi834794.
GrameneiAT5G47450.1; AT5G47450.1; AT5G47450.
KEGGiath:AT5G47450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025628 Genomic DNA. Translation: BAB09071.1.
CP002688 Genomic DNA. Translation: AED95514.1.
BT011212 mRNA. Translation: AAR92248.1.
BT011663 mRNA. Translation: AAS47669.1.
AK229341 mRNA. Translation: BAF01204.1.
RefSeqiNP_199556.1. NM_124117.3.
UniGeneiAt.43789.

3D structure databases

ProteinModelPortaliQ9FGL2.
SMRiQ9FGL2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20042. 2 interactors.
STRINGi3702.AT5G47450.1.

Protein family/group databases

TCDBi1.A.8.10.4. the major intrinsic protein (mip) family.

Proteomic databases

PaxDbiQ9FGL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G47450.1; AT5G47450.1; AT5G47450.
GeneIDi834794.
GrameneiAT5G47450.1; AT5G47450.1; AT5G47450.
KEGGiath:AT5G47450.

Organism-specific databases

TAIRiAT5G47450.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiQ9FGL2.
KOiK09873.
OMAiRSTINGQ.
OrthoDBiEOG09360J95.
PhylomeDBiQ9FGL2.

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Miscellaneous databases

PROiQ9FGL2.

Gene expression databases

GenevisibleiQ9FGL2. AT.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIP23_ARATH
AccessioniPrimary (citable) accession number: Q9FGL2
Secondary accession number(s): Q0WNU5, Q53XE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.