Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9FGI6

- NDUS1_ARATH

UniProt

Q9FGI6 - NDUS1_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

Gene

EMB1467

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 2Fe-2S cluster per subunit.By similarity
Binds 2 4Fe-4S clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi117 – 1171Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi120 – 1201Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi134 – 1341Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi170 – 1701Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi173 – 1731Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi179 – 1791Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi218 – 2181Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi221 – 2211Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi224 – 2241Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi268 – 2681Iron-sulfur 3 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. cellular respiration Source: RefGenome
  3. photorespiration Source: TAIR
  4. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT5G37510-MONOMER.
ARA:GQT-20-MONOMER.
MetaCyc:AT5G37510-MONOMER.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Protein EMBRYO DEFECTIVE 1467
Gene namesi
Name:EMB1467
Ordered Locus Names:At5g37510
ORF Names:MPA22.5
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G37510.

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. mitochondrial membrane Source: TAIR
  3. mitochondrial respiratory chain complex I Source: TAIR
  4. mitochondrion Source: TAIR
  5. respiratory chain complex I Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333MitochondrionBy similarityAdd
BLAST
Chaini34 – 748715NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrialPRO_0000019972Add
BLAST

Proteomic databases

PaxDbiQ9FGI6.
PRIDEiQ9FGI6.

Expressioni

Gene expression databases

GenevestigatoriQ9FGI6.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

BioGridi18980. 1 interaction.
IntActiQ9FGI6. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9FGI6.
SMRiQ9FGI6. Positions 74-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 150792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini287 – 343574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1034.
HOGENOMiHOG000031442.
InParanoidiQ9FGI6.
KOiK03934.
OMAiDYPHLCA.
PhylomeDBiQ9FGI6.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF09326. DUF1982. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FGI6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP
60 70 80 90 100
TQILPPRNPV GGARVHFSNP EDAIEVFVDG YAVKVPKGFT VLQACEVAGV
110 120 130 140 150
DIPRFCYHSR LSIAGNCRMC LVEVEKSPKP VASCAMPALP GMKIKTDTPI
160 170 180 190 200
AKKAREGVME FLLMNHPLDC PICDQGGECD LQDQSMAFGS DRGRFTEMKR
210 220 230 240 250
SVVDKNLGPL VKTVMTRCIQ CTRCVRFASE VAGVQDLGIL GRGSGEEIGT
260 270 280 290 300
YVEKLMTSEL SGNVIDICPV GALTSKPFAF KARNWELKAT ETIDVSDAVG
310 320 330 340 350
SNIRVDSRGP EVMRIIPRLN EDINEEWISD KTRFCYDGLK RQRLSDPMIR
360 370 380 390 400
DSDGRFKAVS WRDALAVVGD IIHQVKPDEI VGVAGQLSDA ESMMVLKDFV
410 420 430 440 450
NRMGSDNVWC EGTAAGVDAD LRYSYLMNTS ISGLENADLF LLIGTQPRVE
460 470 480 490 500
AAMVNARICK TVRASNAKVG YVGPPAEFNY DCKHLGTGPD TLKEIAEGRH
510 520 530 540 550
PFCTALKNAK NPAIIVGAGL FNRTDKNAIL SSVESIAQAN NVVRPDWNGL
560 570 580 590 600
NFLLQYAAQA AALDLGLIQQ SAKALESAKF VYLMGADDVN VDKIPKDAFV
610 620 630 640 650
VYQGHHGDKA VYRANVILPA SAFTEKEGTY ENTEGFTQQT VPAVPTVGDA
660 670 680 690 700
RDDWKIVRAL SEVSGVKLPY NSIEGVRSRI KSVAPNLVHT DEREPAAFGP
710 720 730 740
SLKPECKEAM STTPFQTVVE NFYMTNSITR ASKIMAQCSA VLLKKPFV
Length:748
Mass (Da):81,525
Last modified:October 25, 2004 - v2
Checksum:iC83400F148E73F44
GO

Sequence cautioni

The sequence BAB10668.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB025630 Genomic DNA. Translation: BAB10668.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94200.1.
AY056140 mRNA. Translation: AAL07219.1.
RefSeqiNP_568550.1. NM_123110.2. [Q9FGI6-1]
NP_851103.1. NM_180772.1.
UniGeneiAt.25266.
At.67340.

Genome annotation databases

EnsemblPlantsiAT5G37510.2; AT5G37510.2; AT5G37510. [Q9FGI6-1]
GeneIDi833729.
KEGGiath:AT5G37510.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB025630 Genomic DNA. Translation: BAB10668.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED94200.1 .
AY056140 mRNA. Translation: AAL07219.1 .
RefSeqi NP_568550.1. NM_123110.2. [Q9FGI6-1 ]
NP_851103.1. NM_180772.1.
UniGenei At.25266.
At.67340.

3D structure databases

ProteinModelPortali Q9FGI6.
SMRi Q9FGI6. Positions 74-670.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18980. 1 interaction.
IntActi Q9FGI6. 2 interactions.

Protein family/group databases

TCDBi 3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbi Q9FGI6.
PRIDEi Q9FGI6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G37510.2 ; AT5G37510.2 ; AT5G37510 . [Q9FGI6-1 ]
GeneIDi 833729.
KEGGi ath:AT5G37510.

Organism-specific databases

GeneFarmi 1809.
TAIRi AT5G37510.

Phylogenomic databases

eggNOGi COG1034.
HOGENOMi HOG000031442.
InParanoidi Q9FGI6.
KOi K03934.
OMAi DYPHLCA.
PhylomeDBi Q9FGI6.

Enzyme and pathway databases

BioCyci ARA:AT5G37510-MONOMER.
ARA:GQT-20-MONOMER.
MetaCyc:AT5G37510-MONOMER.

Miscellaneous databases

PROi Q9FGI6.

Gene expression databases

Genevestigatori Q9FGI6.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view ]
Pfami PF09326. DUF1982. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
    Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
    Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 511-523.
    Tissue: Leaf and Stem.
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiNDUS1_ARATH
AccessioniPrimary (citable) accession number: Q9FGI6
Secondary accession number(s): Q940B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 25, 2004
Last modified: October 1, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3