Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9FGI6

- NDUS1_ARATH

UniProt

Q9FGI6 - NDUS1_ARATH

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

Gene

EMB1467

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (25 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi106 – 1061Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi117 – 1171Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi120 – 1201Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi134 – 1341Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi166 – 1661Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi170 – 1701Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi173 – 1731Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi179 – 1791Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi218 – 2181Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi221 – 2211Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi224 – 2241Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi268 – 2681Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro
    2. cellular respiration Source: RefGenome
    3. photorespiration Source: TAIR
    4. response to oxidative stress Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciARA:AT5G37510-MONOMER.
    ARA:GQT-20-MONOMER.
    MetaCyc:AT5G37510-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Protein EMBRYO DEFECTIVE 1467
    Gene namesi
    Name:EMB1467
    Ordered Locus Names:At5g37510
    ORF Names:MPA22.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G37510.

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. mitochondrial membrane Source: TAIR
    3. mitochondrial respiratory chain complex I Source: TAIR
    4. mitochondrion Source: TAIR
    5. respiratory chain complex I Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionBy similarityAdd
    BLAST
    Chaini34 – 748715NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrialPRO_0000019972Add
    BLAST

    Proteomic databases

    PaxDbiQ9FGI6.
    PRIDEiQ9FGI6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FGI6.

    Interactioni

    Subunit structurei

    Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

    Protein-protein interaction databases

    BioGridi18980. 1 interaction.
    IntActiQ9FGI6. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FGI6.
    SMRiQ9FGI6. Positions 74-670.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 150792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini287 – 343574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1034.
    HOGENOMiHOG000031442.
    InParanoidiQ9FGI6.
    KOiK03934.
    OMAiDYPHLCA.
    PhylomeDBiQ9FGI6.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view]
    PfamiPF09326. DUF1982. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FGI6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP    50
    TQILPPRNPV GGARVHFSNP EDAIEVFVDG YAVKVPKGFT VLQACEVAGV 100
    DIPRFCYHSR LSIAGNCRMC LVEVEKSPKP VASCAMPALP GMKIKTDTPI 150
    AKKAREGVME FLLMNHPLDC PICDQGGECD LQDQSMAFGS DRGRFTEMKR 200
    SVVDKNLGPL VKTVMTRCIQ CTRCVRFASE VAGVQDLGIL GRGSGEEIGT 250
    YVEKLMTSEL SGNVIDICPV GALTSKPFAF KARNWELKAT ETIDVSDAVG 300
    SNIRVDSRGP EVMRIIPRLN EDINEEWISD KTRFCYDGLK RQRLSDPMIR 350
    DSDGRFKAVS WRDALAVVGD IIHQVKPDEI VGVAGQLSDA ESMMVLKDFV 400
    NRMGSDNVWC EGTAAGVDAD LRYSYLMNTS ISGLENADLF LLIGTQPRVE 450
    AAMVNARICK TVRASNAKVG YVGPPAEFNY DCKHLGTGPD TLKEIAEGRH 500
    PFCTALKNAK NPAIIVGAGL FNRTDKNAIL SSVESIAQAN NVVRPDWNGL 550
    NFLLQYAAQA AALDLGLIQQ SAKALESAKF VYLMGADDVN VDKIPKDAFV 600
    VYQGHHGDKA VYRANVILPA SAFTEKEGTY ENTEGFTQQT VPAVPTVGDA 650
    RDDWKIVRAL SEVSGVKLPY NSIEGVRSRI KSVAPNLVHT DEREPAAFGP 700
    SLKPECKEAM STTPFQTVVE NFYMTNSITR ASKIMAQCSA VLLKKPFV 748
    Length:748
    Mass (Da):81,525
    Last modified:October 25, 2004 - v2
    Checksum:iC83400F148E73F44
    GO

    Sequence cautioni

    The sequence BAB10668.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025630 Genomic DNA. Translation: BAB10668.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED94200.1.
    AY056140 mRNA. Translation: AAL07219.1.
    RefSeqiNP_568550.1. NM_123110.2. [Q9FGI6-1]
    NP_851103.1. NM_180772.1.
    UniGeneiAt.25266.
    At.67340.

    Genome annotation databases

    EnsemblPlantsiAT5G37510.2; AT5G37510.2; AT5G37510. [Q9FGI6-1]
    GeneIDi833729.
    KEGGiath:AT5G37510.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB025630 Genomic DNA. Translation: BAB10668.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED94200.1 .
    AY056140 mRNA. Translation: AAL07219.1 .
    RefSeqi NP_568550.1. NM_123110.2. [Q9FGI6-1 ]
    NP_851103.1. NM_180772.1.
    UniGenei At.25266.
    At.67340.

    3D structure databases

    ProteinModelPortali Q9FGI6.
    SMRi Q9FGI6. Positions 74-670.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18980. 1 interaction.
    IntActi Q9FGI6. 2 interactions.

    Protein family/group databases

    TCDBi 3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi Q9FGI6.
    PRIDEi Q9FGI6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G37510.2 ; AT5G37510.2 ; AT5G37510 . [Q9FGI6-1 ]
    GeneIDi 833729.
    KEGGi ath:AT5G37510.

    Organism-specific databases

    GeneFarmi 1809.
    TAIRi AT5G37510.

    Phylogenomic databases

    eggNOGi COG1034.
    HOGENOMi HOG000031442.
    InParanoidi Q9FGI6.
    KOi K03934.
    OMAi DYPHLCA.
    PhylomeDBi Q9FGI6.

    Enzyme and pathway databases

    BioCyci ARA:AT5G37510-MONOMER.
    ARA:GQT-20-MONOMER.
    MetaCyc:AT5G37510-MONOMER.

    Miscellaneous databases

    PROi Q9FGI6.

    Gene expression databases

    Genevestigatori Q9FGI6.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view ]
    Pfami PF09326. DUF1982. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
      Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
      Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
      Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 511-523.
      Tissue: Leaf and Stem.
    5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiNDUS1_ARATH
    AccessioniPrimary (citable) accession number: Q9FGI6
    Secondary accession number(s): Q940B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3