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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

Gene

EMB1467

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi106Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi117Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi120Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi134Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi166Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi170Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi173Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi179Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi218Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi221Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi224Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi268Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • ATP synthesis coupled electron transport Source: InterPro
  • cellular respiration Source: GO_Central
  • photorespiration Source: TAIR
  • response to oxidative stress Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT5G37510-MONOMER
MetaCyc:AT5G37510-MONOMER
ReactomeiR-ATH-6799198 Complex I biogenesis

Protein family/group databases

TCDBi3.D.1.6.3 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Protein EMBRYO DEFECTIVE 1467
Gene namesi
Name:EMB1467
Ordered Locus Names:At5g37510
ORF Names:MPA22.5
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G37510
TAIRilocus:2169871 AT5G37510

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33MitochondrionBy similarityAdd BLAST33
ChainiPRO_000001997234 – 748NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrialAdd BLAST715

Proteomic databases

PaxDbiQ9FGI6
PRIDEiQ9FGI6

PTM databases

iPTMnetiQ9FGI6

Expressioni

Gene expression databases

ExpressionAtlasiQ9FGI6 baseline and differential
GenevisibleiQ9FGI6 AT

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

BioGridi18980, 2 interactors
IntActiQ9FGI6, 2 interactors
STRINGi3702.AT5G37510.2

Structurei

3D structure databases

ProteinModelPortaliQ9FGI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 1502Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini150 – 1894Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini287 – 3434Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2282 Eukaryota
COG1034 LUCA
HOGENOMiHOG000031442
InParanoidiQ9FGI6
KOiK03934
OMAiPQASCAM
OrthoDBiEOG093603G8
PhylomeDBiQ9FGI6

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FGI6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP
60 70 80 90 100
TQILPPRNPV GGARVHFSNP EDAIEVFVDG YAVKVPKGFT VLQACEVAGV
110 120 130 140 150
DIPRFCYHSR LSIAGNCRMC LVEVEKSPKP VASCAMPALP GMKIKTDTPI
160 170 180 190 200
AKKAREGVME FLLMNHPLDC PICDQGGECD LQDQSMAFGS DRGRFTEMKR
210 220 230 240 250
SVVDKNLGPL VKTVMTRCIQ CTRCVRFASE VAGVQDLGIL GRGSGEEIGT
260 270 280 290 300
YVEKLMTSEL SGNVIDICPV GALTSKPFAF KARNWELKAT ETIDVSDAVG
310 320 330 340 350
SNIRVDSRGP EVMRIIPRLN EDINEEWISD KTRFCYDGLK RQRLSDPMIR
360 370 380 390 400
DSDGRFKAVS WRDALAVVGD IIHQVKPDEI VGVAGQLSDA ESMMVLKDFV
410 420 430 440 450
NRMGSDNVWC EGTAAGVDAD LRYSYLMNTS ISGLENADLF LLIGTQPRVE
460 470 480 490 500
AAMVNARICK TVRASNAKVG YVGPPAEFNY DCKHLGTGPD TLKEIAEGRH
510 520 530 540 550
PFCTALKNAK NPAIIVGAGL FNRTDKNAIL SSVESIAQAN NVVRPDWNGL
560 570 580 590 600
NFLLQYAAQA AALDLGLIQQ SAKALESAKF VYLMGADDVN VDKIPKDAFV
610 620 630 640 650
VYQGHHGDKA VYRANVILPA SAFTEKEGTY ENTEGFTQQT VPAVPTVGDA
660 670 680 690 700
RDDWKIVRAL SEVSGVKLPY NSIEGVRSRI KSVAPNLVHT DEREPAAFGP
710 720 730 740
SLKPECKEAM STTPFQTVVE NFYMTNSITR ASKIMAQCSA VLLKKPFV
Length:748
Mass (Da):81,525
Last modified:October 25, 2004 - v2
Checksum:iC83400F148E73F44
GO

Sequence cautioni

The sequence BAB10668 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025630 Genomic DNA Translation: BAB10668.1 Sequence problems.
CP002688 Genomic DNA Translation: AED94200.1
AY056140 mRNA Translation: AAL07219.1
RefSeqiNP_568550.1, NM_123110.2 [Q9FGI6-1]
NP_851103.1, NM_180772.2
UniGeneiAt.25266
At.67340

Genome annotation databases

EnsemblPlantsiAT5G37510.2; AT5G37510.2; AT5G37510 [Q9FGI6-1]
GeneIDi833729
GrameneiAT5G37510.2; AT5G37510.2; AT5G37510 [Q9FGI6-1]
KEGGiath:AT5G37510

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNDUS1_ARATH
AccessioniPrimary (citable) accession number: Q9FGI6
Secondary accession number(s): Q940B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 25, 2004
Last modified: April 25, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health