NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial precursor

Contribute

Send feedback

Read comments (?) or add your own

Q9FGI6 (NDUS1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Protein EMBRYO DEFECTIVE 1467

Gene names

Name:	EMB1467
Ordered Locus Names:	At5g37510
ORF Names:	MPA22.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. UniProtKB Q43644 Contains 1 2Fe-2S ferredoxin-type domain.
Sequence caution	The sequence BAB10668.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro cellular respiration Inferred from Biological aspect of Ancestor. Source: RefGenome photorespiration Traceable author statement PubMed 16407270. Source: TAIR response to oxidative stress Inferred from direct assay PubMed 12492832. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR mitochondrial respiratory chain complex I Inferred from direct assay PubMed 18189341 PubMed 20197505. Source: TAIR
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from Biological aspect of Ancestor. Source: RefGenome electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FGI6-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 33

Mitochondrion By similarity UniProtKB Q43644

Chain

34 – 748

715

NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

PRO_0000019972

Regions

Domain

72 – 150

2Fe-2S ferredoxin-type

Sites

Metal binding

106

Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644

Metal binding

117

Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644

Metal binding

120

Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644

Metal binding

134

Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644

Metal binding

166

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

170

Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644

Metal binding

173

Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644

Metal binding

179

Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644

Metal binding

218

Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644

Metal binding

221

Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644

Metal binding

224

Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644

Metal binding

268

Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified October 25, 2004. Version 2.
Checksum: C83400F148E73F44
Show »

FASTA

748

81,525

        10         20         30         40         50         60 
MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP TQILPPRNPV 

        70         80         90        100        110        120 
GGARVHFSNP EDAIEVFVDG YAVKVPKGFT VLQACEVAGV DIPRFCYHSR LSIAGNCRMC 

       130        140        150        160        170        180 
LVEVEKSPKP VASCAMPALP GMKIKTDTPI AKKAREGVME FLLMNHPLDC PICDQGGECD 

       190        200        210        220        230        240 
LQDQSMAFGS DRGRFTEMKR SVVDKNLGPL VKTVMTRCIQ CTRCVRFASE VAGVQDLGIL 

       250        260        270        280        290        300 
GRGSGEEIGT YVEKLMTSEL SGNVIDICPV GALTSKPFAF KARNWELKAT ETIDVSDAVG 

       310        320        330        340        350        360 
SNIRVDSRGP EVMRIIPRLN EDINEEWISD KTRFCYDGLK RQRLSDPMIR DSDGRFKAVS 

       370        380        390        400        410        420 
WRDALAVVGD IIHQVKPDEI VGVAGQLSDA ESMMVLKDFV NRMGSDNVWC EGTAAGVDAD 

       430        440        450        460        470        480 
LRYSYLMNTS ISGLENADLF LLIGTQPRVE AAMVNARICK TVRASNAKVG YVGPPAEFNY 

       490        500        510        520        530        540 
DCKHLGTGPD TLKEIAEGRH PFCTALKNAK NPAIIVGAGL FNRTDKNAIL SSVESIAQAN 

       550        560        570        580        590        600 
NVVRPDWNGL NFLLQYAAQA AALDLGLIQQ SAKALESAKF VYLMGADDVN VDKIPKDAFV 

       610        620        630        640        650        660 
VYQGHHGDKA VYRANVILPA SAFTEKEGTY ENTEGFTQQT VPAVPTVGDA RDDWKIVRAL 

       670        680        690        700        710        720 
SEVSGVKLPY NSIEGVRSRI KSVAPNLVHT DEREPAAFGP SLKPECKEAM STTPFQTVVE 

       730        740 
NFYMTNSITR ASKIMAQCSA VLLKKPFV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis." Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P. Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 511-523. Tissue: Leaf and Stem.
[5]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025630 Genomic DNA. Translation: BAB10668.1. Sequence problems. CP002688 Genomic DNA. Translation: AED94200.1. AY056140 mRNA. Translation: AAL07219.1.
IPI	IPI00535282.
RefSeq	NP_568550.1. NM_123110.2. NP_851103.1. NM_180772.1.
UniGene	At.25266. At.67340.
3D structure databases
ProteinModelPortal	Q9FGI6.
SMR	Q9FGI6. Positions 74-670.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FGI6. 2 interactions.
Protein family/group databases
TCDB	3.D.1.6.3. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PaxDb	Q9FGI6.
PRIDE	Q9FGI6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G37510.2; AT5G37510.2; AT5G37510.
GeneID	833729.
KEGG	ath:AT5G37510.
Organism-specific databases
GeneFarm	1809.
TAIR	At5g37510.
Phylogenomic databases
eggNOG	COG1034.
HOGENOM	HOG000031442.
InParanoid	Q9FGI6.
KO	K03934.
OMA	AMGYGRD.
PhylomeDB	Q9FGI6.
ProtClustDB	CLSN2689894.
Enzyme and pathway databases
BioCyc	ARA:AT5G37510-MONOMER. MetaCyc:AT5G37510-MONOMER.
Gene expression databases
ArrayExpress	Q9FGI6.
Genevestigator	Q9FGI6.
GermOnline	AT5G37510. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR012675. Beta-grasp_dom. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SMART	SM00929. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_ARATH

Accession

Primary (citable) accession number: Q9FGI6
Secondary accession number(s): Q940B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents