Reviewed,
UniProtKB/Swiss-Prot Q9FGI6 (NDUS1_ARATH)
Last modified
June 16, 2009.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial EC=1.6.5.3 EC=1.6.99.3 Alternative name(s): 75 kDa mitochondrial complex I subunit Complex I-75kD Short name=CI-75kD Protein EMBRYO DEFECTIVE 1467 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 748 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity. |
| Catalytic activity | NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor. |
| Cofactor | Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity. |
| Subunit structure | Complex I is composed of about 30 different subunits By similarity. UniProtKB Q43644 |
| Subcellular location | Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity. |
| Sequence similarities | Belongs to the complex I 75 kDa subunit family. UniProtKB Q43644 Contains 1 2Fe-2S ferredoxin-type domain. |
| Sequence caution | The sequence BAB10668.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: Q9FGI6-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 33 | 33 | Mitochondrion By similarity UniProtKB Q43644 | ||||||
| Chain | 34 – 748 | 715 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | PRO_0000019972 | |||||
Regions | |||||||||
| Domain | 72 – 150 | 79 | 2Fe-2S ferredoxin-type | ||||||
Sites | |||||||||
| Metal binding | 106 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 117 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 120 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 134 | 1 | Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 166 | 1 | Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity | ||||||
| Metal binding | 170 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 173 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 179 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 218 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 221 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 224 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
| Metal binding | 268 | 1 | Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644 | ||||||
Sequences
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References
| [1] | "Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [3] | "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis." Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P. Plant Physiol. 127:1694-1710(2001) [PubMed: 11743114] [Abstract] Cited for: PROTEIN SEQUENCE OF 511-523. Tissue: Leaf and Stem. |
| [4] | "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
Cross-references
Sequence databases | |
|---|---|
| AB025630 Genomic DNA. Translation: BAB10668.1. Sequence problems. AY056140 mRNA. Translation: AAL07219.1. | |
| IPI | IPI00535282. |
| RefSeq | NP_568550.1. |
| UniGene | At.25266 At.67340 |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| TCDB | 3.D.1.6.3. proton-translocating NADH dehydrogenase (NDH) family. |
Proteomic databases | |
| PRIDE | Q9FGI6. |
Genome annotation databases | |
| GeneID | 833729. |
| GenomeReviews | Gene locus AT5G37510 in contig BA000015_GR. |
| KEGG | ath:AT5G37510. |
| NMPDR | fig|3702.1.peg.25437. |
Organism-specific databases | |
| GeneFarm | 1809. |
| TAIR | At5g37510. |
Phylogenomic databases | |
| OMA | Q9FGI6. RMSSGVT. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT5G37510-MON. |
| BRENDA | 1.6.5.3. 302. 1.6.99.3. 302. |
Gene expression databases | |
| ArrayExpress | Q9FGI6. |
| GermOnline | AT5G37510. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view] |
| Pfam | PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01973. NuoG. 1 hit. |
| PROSITE | PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NDUS1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9FGI6 Secondary accession number(s): Q940B1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


