Reviewed, UniProtKB/Swiss-Prot Q9FGI6 (NDUS1_ARATH)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    75 kDa mitochondrial complex I subunit
    Complex I-75kD
      Short name=CI-75kD
    Protein EMBRYO DEFECTIVE 1467

Gene names

Name:	EMB1467
Ordered Locus Names:	At5g37510
ORF Names:	MPA22.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 30 different subunits By similarity. UniProtKB Q43644
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. UniProtKB Q43644 Contains 1 2Fe-2S ferredoxin-type domain.
Sequence caution	The sequence BAB10668.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro photorespiration Traceable author statement. Source: TAIR response to oxidative stress Inferred from direct assay. Source: TAIR transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain complex I Inferred from direct assay. Source: TAIR
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FGI6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion By similarity UniProtKB Q43644
	Chain	34 – 748	715	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial		PRO_0000019972
Regions
	Domain	72 – 150	79	2Fe-2S ferredoxin-type
Sites
	Metal binding	106	1	Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644
	Metal binding	117	1	Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644
	Metal binding	120	1	Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644
	Metal binding	134	1	Iron-sulfur 1 (2Fe-2S) By similarity UniProtKB Q43644
	Metal binding	166	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	170	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644
	Metal binding	173	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644
	Metal binding	179	1	Iron-sulfur 2 (4Fe-4S) By similarity UniProtKB Q43644
	Metal binding	218	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644
	Metal binding	221	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644
	Metal binding	224	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644
	Metal binding	268	1	Iron-sulfur 3 (4Fe-4S) By similarity UniProtKB Q43644

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: C83400F148E73F44	FASTA	748	81,525
	10 20 30 40 50 60 MGLGILASRT IRPASRLLQS QTSNFFLRTI VSKPELQSPE SAAVSEPEPP TQILPPRNPV 70 80 90 100 110 120 GGARVHFSNP EDAIEVFVDG YAVKVPKGFT VLQACEVAGV DIPRFCYHSR LSIAGNCRMC 130 140 150 160 170 180 LVEVEKSPKP VASCAMPALP GMKIKTDTPI AKKAREGVME FLLMNHPLDC PICDQGGECD 190 200 210 220 230 240 LQDQSMAFGS DRGRFTEMKR SVVDKNLGPL VKTVMTRCIQ CTRCVRFASE VAGVQDLGIL 250 260 270 280 290 300 GRGSGEEIGT YVEKLMTSEL SGNVIDICPV GALTSKPFAF KARNWELKAT ETIDVSDAVG 310 320 330 340 350 360 SNIRVDSRGP EVMRIIPRLN EDINEEWISD KTRFCYDGLK RQRLSDPMIR DSDGRFKAVS 370 380 390 400 410 420 WRDALAVVGD IIHQVKPDEI VGVAGQLSDA ESMMVLKDFV NRMGSDNVWC EGTAAGVDAD 430 440 450 460 470 480 LRYSYLMNTS ISGLENADLF LLIGTQPRVE AAMVNARICK TVRASNAKVG YVGPPAEFNY 490 500 510 520 530 540 DCKHLGTGPD TLKEIAEGRH PFCTALKNAK NPAIIVGAGL FNRTDKNAIL SSVESIAQAN 550 560 570 580 590 600 NVVRPDWNGL NFLLQYAAQA AALDLGLIQQ SAKALESAKF VYLMGADDVN VDKIPKDAFV 610 620 630 640 650 660 VYQGHHGDKA VYRANVILPA SAFTEKEGTY ENTEGFTQQT VPAVPTVGDA RDDWKIVRAL 670 680 690 700 710 720 SEVSGVKLPY NSIEGVRSRI KSVAPNLVHT DEREPAAFGP SLKPECKEAM STTPFQTVVE 730 740 NFYMTNSITR ASKIMAQCSA VLLKKPFV

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis." Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P. Plant Physiol. 127:1694-1710(2001) [PubMed: 11743114] [Abstract] Cited for: PROTEIN SEQUENCE OF 511-523. Tissue: Leaf and Stem.
[4]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB025630 Genomic DNA. Translation: BAB10668.1. Sequence problems. AY056140 mRNA. Translation: AAL07219.1.
IPI	IPI00535282.
RefSeq	NP_568550.1.
UniGene	At.25266 At.67340
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.6.3. proton-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PRIDE	Q9FGI6.
Genome annotation databases
GeneID	833729.
GenomeReviews	Gene locus AT5G37510 in contig BA000015_GR.
KEGG	ath:AT5G37510.
NMPDR	fig|3702.1.peg.25437.
Organism-specific databases
GeneFarm	1809.
TAIR	At5g37510.
Phylogenomic databases
OMA	Q9FGI6. RMSSGVT.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G37510-MON.
BRENDA	1.6.5.3. 302. 1.6.99.3. 302.
Gene expression databases
ArrayExpress	Q9FGI6.
GermOnline	AT5G37510. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_ARATH

Accession

Primary (citable) accession number: Q9FGI6
Secondary accession number(s): Q940B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)