Q9FG93 (MNS4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-mannosidase I MNS4
EC=3.2.1.-

Gene names

Name:	MNS4
Ordered Locus Names:	At5g43710
ORF Names:	MQD19.4, MQO24.4

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in N-glycan processing By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	Probable alpha-mannosidase I MNS4		PRO_0000397936
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 624	596	Lumenal Potential
Sites
	Active site	122	1	Proton donor By similarity
	Active site	262	1	By similarity
	Active site	376	1	By similarity
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	494	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FG93 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 07BF24D5BB347CA9	FASTA	624	70,096
	10 20 30 40 50 60 MDSNFKWLLF AILISLTFSG FVLHHGVLAE SVKPDEAKQL RDEVRGMFYH AFDGYMNNAF 70 80 90 100 110 120 PLDELRPLSC QGEDTLGGYA LTLIDSLDTL ALLGDRERFT SSVEWIGKNL QFNINKTVSV 130 140 150 160 170 180 FETTIRVLGG LLSAHLIASD YATGMRIPSY NNELLVLAEN LARRMLPAFD TPTGIPFGSV 190 200 210 220 230 240 NLMYGVDKHE SKITSTAGGG TLSLEFGVLS RLTNDPVFEQ VAKNAVRGLW ARRSNLDLVG 250 260 270 280 290 300 AHINVFTGEW TQKDAGIGTS IDSFYEYLLK AYILFGDEEY LYIFQEAYRS AMQYLHKDPW 310 320 330 340 350 360 YVEVNMDSAA IVWPVFNSLQ AFWPGLQVLA GDVDPAIRTH TAFFSVWKRY GFTPEGFNLA 370 380 390 400 410 420 TLSVQYGQKS YPLRPELIES TYWLYKATRD PRYLDAGRDF VASLQYGAKC PCGYCHITDV 430 440 450 460 470 480 ELHKQEDHME SFFLAETVKY LWLLFDLAVD SDNLVDNGPY KYIFSTEGHL LPITPQISLA 490 500 510 520 530 540 REHCSYFGGY CPSNSTKLEQ EVLGEDSSND DHSNDYPYHE SFPVTGLIKG LCPGLTHAQK 550 560 570 580 590 600 YGFSYVLPEK TDREDVNQPK PVVTSSSIVL ISDQTVEKRP QEEEGFTSQS EPIMTISGGS 610 620 SNDQTGQELT LLESETDDQR SYSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana." Liebminger E., Huttner S., Vavra U., Fischl R., Schoberer J., Grass J., Blaukopf C., Seifert G.J., Altmann F., Mach L., Strasser R. Plant Cell 21:3850-3867(2009) [PubMed: 20023195] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026651 Genomic DNA. Translation: BAB11298.1. CP002688 Genomic DNA. Translation: AED94999.1. AF370128 mRNA. Translation: AAK43943.1. AY040044 mRNA. Translation: AAK64102.1.
IPI	IPI00541320.
RefSeq	NP_199184.1. NM_123737.2.
UniGene	At.23954.
3D structure databases
HSSP	HSSP built from PDB template 1FMI based on UniProtKB Q9UKM7.
ProteinModelPortal	Q9FG93.
SMR	Q9FG93. Positions 31-474.
ModBase	Search...
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Proteomic databases
PRIDE	Q9FG93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G43710.1; AT5G43710.1; AT5G43710.
GeneID	834391.
GenomeReviews	Gene locus AT5G43710 in contig BA000015_GR.
KEGG	ath:AT5G43710.
NMPDR	fig|3702.1.peg.26114.
Organism-specific databases
TAIR	At5g43710.
Phylogenomic databases
eggNOG	KOG2429.
GeneTree	EPGT00050000018377.
HOGENOM	HBG562188.
InParanoid	Q9FG93.
OMA	IGTSIDS.
PhylomeDB	Q9FG93.
ProtClustDB	CLSN2679415.
Gene expression databases
ArrayExpress	Q9FG93.
Genevestigator	Q9FG93.
Family and domain databases
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
Gene3D	G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
KO	K10085.
PANTHER	PTHR11742. Glyco_hydro_47. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNS4_ARATH

Accession

Primary (citable) accession number: Q9FG93

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 1, 2001
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program