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Protein

Methylthioalkylmalate synthase 1, chloroplastic

Gene

MAM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Determines the side chain length of aliphatic glucosinolate structures. Catalyzes exclusively the condensation reactions of both the first and second methionine carbon chain elongation.2 Publications

Miscellaneous

The 5'-part of the gene encoding this protein is deleted in cv. Bl-0, cv. Di-G, cv. Landsberg erecta and cv. Petergof, while the complete gene is missing in cv. Ka-0, cv. Lip-0, cv. No-0, cv. Sei-0, cv. Tsu-1 and cv. Wl-0.

Catalytic activityi

An omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O = a 2-(omega-methylthio)alkylmalate + CoA.1 Publication

Cofactori

Mn2+Note: Manganese or any other divalent metal ion, except copper or zinc.

Enzyme regulationi

1 mM DTT required for activity. Activated by ATP and inhibited by iodoacetamide.1 Publication

Kineticsi

  1. KM=3.0 mM for 4-methylthio-2-oxobutanoic acid1 Publication
  2. KM=11.2 mM for 2-oxohexanoic acid1 Publication
  3. KM=0.64 mM for 5-methylthio-2-oxopentanoic acid1 Publication
  4. KM=7.5 mM for 2-oxoheptanoic acid1 Publication
  5. KM=245 µM for acetyl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Temperature dependencei

    Optimum temperature is 32 degrees Celsius.1 Publication

    GO - Molecular functioni

    • 2-(2'-methylthio)ethylmalate synthase activity Source: TAIR

    GO - Biological processi

    • carboxylic acid metabolic process Source: InterPro
    • glucosinolate biosynthetic process Source: TAIR
    • response to insect Source: TAIR
    • response to water deprivation Source: TAIR

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    BioCyciMetaCyc:AT5G23010-MONOMER
    BRENDAi2.3.3.13 399

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylthioalkylmalate synthase 1, chloroplastic (EC:2.3.3.171 Publication)
    Alternative name(s):
    2-isopropylmalate synthase 3
    Gene namesi
    Name:MAM1
    Synonyms:IMS3, IPMS_AT2, MAM-L, MAML
    Ordered Locus Names:At5g23010
    ORF Names:T20O7.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G23010
    TAIRilocus:2181151 AT5G23010

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi102S → F in gsm1-1; loss of conversion of C3 to C4 glucosinolates. 1 Publication1
    Mutagenesisi290A → T in gsm1-2; loss of conversion of C3 to C4 glucosinolates. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 49ChloroplastSequence analysisAdd BLAST49
    ChainiPRO_000031584150 – 506Methylthioalkylmalate synthase 1, chloroplasticAdd BLAST457

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei98PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9FG67
    PRIDEiQ9FG67

    PTM databases

    iPTMnetiQ9FG67

    Expressioni

    Tissue specificityi

    Highly expressed in leaves, flowers, roots and siliques. Not detected in flowers in PubMed:12432038.2 Publications

    Gene expression databases

    ExpressionAtlasiQ9FG67 baseline and differential
    GenevisibleiQ9FG67 AT

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT5G23010.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FG67
    SMRiQ9FG67
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini85 – 359Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST275

    Domaini

    The N-terminal part of the protein controls substrate specificity.

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG2367 Eukaryota
    COG0119 LUCA
    HOGENOMiHOG000046861
    InParanoidiQ9FG67
    KOiK15741
    OMAiQIEAGFP
    OrthoDBiEOG093608QE
    PhylomeDBiQ9FG67

    Family and domain databases

    Gene3Di3.20.20.701 hit
    InterProiView protein in InterPro
    IPR002034 AIPM/Hcit_synth_CS
    IPR013785 Aldolase_TIM
    IPR000891 PYR_CT
    PfamiView protein in Pfam
    PF00682 HMGL-like, 1 hit
    PROSITEiView protein in PROSITE
    PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
    PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
    PS50991 PYR_CT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FG67-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASSLLTSSV MIPTTGSTVV GRSVLPFQSS LHSLRLTHSY KNPALFISCC
    60 70 80 90 100
    SSVSKNAATS STDLKPVVER WPEYIPNKLP DGNYVRVFDT TLRDGEQSPG
    110 120 130 140 150
    GSLTPPQKLE IARQLAKLRV DIMEVGFPGS SEEELETIKT IAKTVGNEVD
    160 170 180 190 200
    EETGYVPVIC AIARCKHRDI EATWEALKYA KRPRILVFTS TSDIHMKYKL
    210 220 230 240 250
    KKTQEEVIEM AVSSIRFAKS LGFNDIQFGC EDGGRSDKDF LCKILGEAIK
    260 270 280 290 300
    AGVTVVTIGD TVGINMPHEY GELVTYLKAN TPGIDDVVVA VHCHNDLGLA
    310 320 330 340 350
    TANSIAGIRA GARQVEVTIN GIGERSGNAS LEEVVMALKC RGAYVINGVY
    360 370 380 390 400
    TKIDTRQIMA TSKMVQEYTG LYVQAHKPIV GANCFVHESG IHQDGILKNR
    410 420 430 440 450
    STYEILSPED IGIVKSQNSG LVLGKLSGRH AVKDRLKELG YELDDEKLNA
    460 470 480 490 500
    VFSLFRDLTK NKKRITDADL KALVTSSDEI SLEKLNGANG LKSNGYIPVP

    QVSSNV
    Length:506
    Mass (Da):55,125
    Last modified:March 1, 2001 - v1
    Checksum:iA3F4CC32508CAE49
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti10V → G in strain: cv. Sorbo. 1
    Natural varianti42N → K in strain: cv. Ema-1 and cv. Pla-0. 1
    Natural varianti44A → T in strain: cv. Sorbo. 1
    Natural varianti505N → T in strain: cv. Sorbo. 1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ131517 Genomic DNA Translation: CAC80102.1
    AY049037 mRNA Translation: AAL10687.1
    AJ486882 mRNA Translation: CAD31140.1
    AJ486883 mRNA Translation: CAD31141.1
    AJ486884 mRNA Translation: CAD31142.1
    AJ486885 mRNA Translation: CAD31143.1
    AJ486886 mRNA Translation: CAD31144.1
    AJ486887 mRNA Translation: CAD31145.1
    AJ486888 mRNA Translation: CAD31146.1
    AM180572 Genomic DNA Translation: CAJ55504.1
    AB026660 Genomic DNA Translation: BAB08874.1
    CP002688 Genomic DNA Translation: AED93107.1
    AY054203 mRNA Translation: AAL06864.1
    AY070471 mRNA Translation: AAL49937.1
    AY149926 mRNA Translation: AAN31080.1
    RefSeqiNP_197692.1, NM_122207.3
    UniGeneiAt.7567

    Genome annotation databases

    EnsemblPlantsiAT5G23010.1; AT5G23010.1; AT5G23010
    AT5G23010.2; AT5G23010.2; AT5G23010
    GeneIDi832365
    GrameneiAT5G23010.1; AT5G23010.1; AT5G23010
    AT5G23010.2; AT5G23010.2; AT5G23010
    KEGGiath:AT5G23010

    Similar proteinsi

    Entry informationi

    Entry nameiMAM1_ARATH
    AccessioniPrimary (citable) accession number: Q9FG67
    Secondary accession number(s): Q70YX4, Q70YX9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: April 25, 2018
    This is version 114 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome