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Q9FG67 (MAM1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylthioalkylmalate synthase 1, chloroplastic

EC=2.3.3.-
Alternative name(s):
2-isopropylmalate synthase 3
Gene names
Name:MAM1
Synonyms:IMS3, IPMS_AT2, MAM-L, MAML
Ordered Locus Names:At5g23010
ORF Names:T20O7.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Determines the side chain length of aliphatic glucosinolate structures. Catalyzes exclusively the condensation reactions of both the first and second methionine carbon chain elongation. Ref.4 Ref.9

Cofactor

Manganese or any other divalent metal ion, except copper or zinc.

Enzyme regulation

1 mM DTT required for activity. Activated by ATP and inhibited by iodoacetamide. Ref.9

Subunit structure

Monomer. Ref.9

Subcellular location

Plastidchloroplast Potential.

Tissue specificity

Highly expressed in leaves, flowers, roots and siliques. Not detected in flowers in Ref.2. Ref.2 Ref.10

Domain

The N-terminal part of the protein controls substrate specificity.

Miscellaneous

The 5'-part of the gene encoding this protein is deleted in cv. Bl-0, cv. Di-G, cv. Landsberg erecta and cv. Petergof, while the complete gene is missing in cv. Ka-0, cv. Lip-0, cv. No-0, cv. Sei-0, cv. Tsu-1 and cv. Wl-0.

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family.

Biophysicochemical properties

Kinetic parameters:

KM=3.0 mM for 4-methylthio-2-oxobutanoic acid Ref.9

KM=11.2 mM for 2-oxohexanoic acid

KM=0.64 mM for 5-methylthio-2-oxopentanoic acid

KM=7.5 mM for 2-oxoheptanoic acid

KM=245 µM for acetyl-CoA

pH dependence:

Optimum pH is 8.5.

Temperature dependence:

Optimum temperature is 32 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Potential
Chain50 – 506457Methylthioalkylmalate synthase 1, chloroplastic
PRO_0000315841

Amino acid modifications

Modified residue981Phosphoserine Ref.11

Natural variations

Natural variant101V → G in strain: cv. Sorbo.
Natural variant421N → K in strain: cv. Ema-1 and cv. Pla-0.
Natural variant441A → T in strain: cv. Sorbo.
Natural variant5051N → T in strain: cv. Sorbo.

Experimental info

Mutagenesis1021S → F in gsm1-1; loss of conversion of C3 to C4 glucosinolates. Ref.1
Mutagenesis2901A → T in gsm1-2; loss of conversion of C3 to C4 glucosinolates. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9FG67 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: A3F4CC32508CAE49

FASTA50655,125
        10         20         30         40         50         60 
MASSLLTSSV MIPTTGSTVV GRSVLPFQSS LHSLRLTHSY KNPALFISCC SSVSKNAATS 

        70         80         90        100        110        120 
STDLKPVVER WPEYIPNKLP DGNYVRVFDT TLRDGEQSPG GSLTPPQKLE IARQLAKLRV 

       130        140        150        160        170        180 
DIMEVGFPGS SEEELETIKT IAKTVGNEVD EETGYVPVIC AIARCKHRDI EATWEALKYA 

       190        200        210        220        230        240 
KRPRILVFTS TSDIHMKYKL KKTQEEVIEM AVSSIRFAKS LGFNDIQFGC EDGGRSDKDF 

       250        260        270        280        290        300 
LCKILGEAIK AGVTVVTIGD TVGINMPHEY GELVTYLKAN TPGIDDVVVA VHCHNDLGLA 

       310        320        330        340        350        360 
TANSIAGIRA GARQVEVTIN GIGERSGNAS LEEVVMALKC RGAYVINGVY TKIDTRQIMA 

       370        380        390        400        410        420 
TSKMVQEYTG LYVQAHKPIV GANCFVHESG IHQDGILKNR STYEILSPED IGIVKSQNSG 

       430        440        450        460        470        480 
LVLGKLSGRH AVKDRLKELG YELDDEKLNA VFSLFRDLTK NKKRITDADL KALVTSSDEI 

       490        500 
SLEKLNGANG LKSNGYIPVP QVSSNV 

« Hide

References

« Hide 'large scale' references
[1]"A gene controlling variation in Arabidopsis glucosinolate composition is part of the methionine chain elongation pathway."
Kroymann J., Textor S., Tokuhisa J.G., Falk K.L., Bartram S., Gershenzon J., Mitchell-Olds T.
Plant Physiol. 127:1077-1088(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF SER-102 AND ALA-290, CHARACTERIZATION.
Strain: cv. Columbia.
[2]"Isolation and expression analysis of the isopropylmalate synthase gene family of Arabidopsis thaliana."
Junk D.J., Mourad G.S.
J. Exp. Bot. 53:2453-2454(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]"Evolutionary dynamics of an Arabidopsis insect resistance quantitative trait locus."
Kroymann J., Donnerhacke S., Schnabelrauch D., Mitchell-Olds T.
Proc. Natl. Acad. Sci. U.S.A. 100:14587-14592(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS.
Strain: cv. Aa-0, cv. Ag-0, cv. Ema-1, cv. Gy-0, cv. Landsberg erecta, cv. Mt-0, cv. Pla-0 and cv. Sorbo.
[4]"Positive selection driving diversification in plant secondary metabolism."
Benderoth M., Textor S., Windsor A.J., Mitchell-Olds T., Gershenzon J., Kroymann J.
Proc. Natl. Acad. Sci. U.S.A. 103:9118-9123(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: cv. Sorbo.
[5]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[6]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[7]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[8]"Alpha-keto acid elongation and glucosinolate biosynthesis in Arabidopsis thaliana."
Campos de Quiros H., Magrath R., McCallum D., Kroymann J., Scnabelrauch D., Mitchell-Olds T., Mithen R.
Theor. Appl. Genet. 101:429-437(2000) [AGRICOLA] [Europe PMC]
Cited for: IDENTIFICATION.
[9]"Biosynthesis of methionine-derived glucosinolates in Arabidopsis thaliana: recombinant expression and characterization of methylthioalkylmalate synthase, the condensing enzyme of the chain-elongation cycle."
Textor S., Bartram S., Kroymann J., Falk K.L., Hick A., Pickett J.A., Gershenzon J.
Planta 218:1026-1035(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT.
Strain: cv. Columbia.
[10]"MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis."
Textor S., de Kraker J.-W., Hause B., Gershenzon J., Tokuhisa J.G.
Plant Physiol. 144:60-71(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[11]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ131517 Genomic DNA. Translation: CAC80102.1.
AY049037 mRNA. Translation: AAL10687.1.
AJ486882 mRNA. Translation: CAD31140.1.
AJ486883 mRNA. Translation: CAD31141.1.
AJ486884 mRNA. Translation: CAD31142.1.
AJ486885 mRNA. Translation: CAD31143.1.
AJ486886 mRNA. Translation: CAD31144.1.
AJ486887 mRNA. Translation: CAD31145.1.
AJ486888 mRNA. Translation: CAD31146.1.
AM180572 Genomic DNA. Translation: CAJ55504.1.
AB026660 Genomic DNA. Translation: BAB08874.1.
CP002688 Genomic DNA. Translation: AED93107.1.
AY054203 mRNA. Translation: AAL06864.1.
AY070471 mRNA. Translation: AAL49937.1.
AY149926 mRNA. Translation: AAN31080.1.
RefSeqNP_197692.1. NM_122207.3.
UniGeneAt.7567.

3D structure databases

ProteinModelPortalQ9FG67.
SMRQ9FG67. Positions 58-473.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9FG67.
PRIDEQ9FG67.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G23010.1; AT5G23010.1; AT5G23010.
GeneID832365.
KEGGath:AT5G23010.

Organism-specific databases

TAIRAT5G23010.

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000046861.
InParanoidQ9FG67.
KOK15741.
OMALQININT.
PhylomeDBQ9FG67.
ProtClustDBPLN03228.

Enzyme and pathway databases

BioCycMetaCyc:AT5G23010-MONOMER.
BRENDA2.3.3.13. 399.

Gene expression databases

ArrayExpressQ9FG67.
GenevestigatorQ9FG67.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
[Graphical view]
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAM1_ARATH
AccessionPrimary (citable) accession number: Q9FG67
Secondary accession number(s): Q70YX4, Q70YX9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: March 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names