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Protein

Methylthioalkylmalate synthase 1, chloroplastic

Gene

MAM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Determines the side chain length of aliphatic glucosinolate structures. Catalyzes exclusively the condensation reactions of both the first and second methionine carbon chain elongation.2 Publications

Cofactori

Mn2+Note: Manganese or any other divalent metal ion, except copper or zinc.

Enzyme regulationi

1 mM DTT required for activity. Activated by ATP and inhibited by iodoacetamide.1 Publication

Kineticsi

  1. KM=3.0 mM for 4-methylthio-2-oxobutanoic acid1 Publication
  2. KM=11.2 mM for 2-oxohexanoic acid1 Publication
  3. KM=0.64 mM for 5-methylthio-2-oxopentanoic acid1 Publication
  4. KM=7.5 mM for 2-oxoheptanoic acid1 Publication
  5. KM=245 µM for acetyl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Temperature dependencei

    Optimum temperature is 32 degrees Celsius.1 Publication

    GO - Molecular functioni

    • 2-(2'-methylthio)ethylmalate synthase activity Source: TAIR

    GO - Biological processi

    • carboxylic acid metabolic process Source: InterPro
    • glucosinolate biosynthetic process Source: TAIR
    • response to insect Source: TAIR
    • response to water deprivation Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:AT5G23010-MONOMER.
    BRENDAi2.3.3.13. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylthioalkylmalate synthase 1, chloroplastic (EC:2.3.3.-)
    Alternative name(s):
    2-isopropylmalate synthase 3
    Gene namesi
    Name:MAM1
    Synonyms:IMS3, IPMS_AT2, MAM-L, MAML
    Ordered Locus Names:At5g23010
    ORF Names:T20O7.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G23010.

    Subcellular locationi

    GO - Cellular componenti

    • chloroplast Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi102S → F in gsm1-1; loss of conversion of C3 to C4 glucosinolates. 1 Publication1
    Mutagenesisi290A → T in gsm1-2; loss of conversion of C3 to C4 glucosinolates. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 49ChloroplastSequence analysisAdd BLAST49
    ChainiPRO_000031584150 – 506Methylthioalkylmalate synthase 1, chloroplasticAdd BLAST457

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei98PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9FG67.
    PRIDEiQ9FG67.

    PTM databases

    iPTMnetiQ9FG67.

    Expressioni

    Tissue specificityi

    Highly expressed in leaves, flowers, roots and siliques. Not detected in flowers in PubMed:12432038.2 Publications

    Gene expression databases

    GenevisibleiQ9FG67. AT.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT5G23010.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FG67.
    SMRiQ9FG67.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini85 – 359Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST275

    Domaini

    The N-terminal part of the protein controls substrate specificity.

    Sequence similaritiesi

    Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG2367. Eukaryota.
    COG0119. LUCA.
    HOGENOMiHOG000046861.
    InParanoidiQ9FG67.
    KOiK15741.
    OMAiDTVGVMH.
    OrthoDBiEOG093608QE.
    PhylomeDBiQ9FG67.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    [Graphical view]
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FG67-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASSLLTSSV MIPTTGSTVV GRSVLPFQSS LHSLRLTHSY KNPALFISCC
    60 70 80 90 100
    SSVSKNAATS STDLKPVVER WPEYIPNKLP DGNYVRVFDT TLRDGEQSPG
    110 120 130 140 150
    GSLTPPQKLE IARQLAKLRV DIMEVGFPGS SEEELETIKT IAKTVGNEVD
    160 170 180 190 200
    EETGYVPVIC AIARCKHRDI EATWEALKYA KRPRILVFTS TSDIHMKYKL
    210 220 230 240 250
    KKTQEEVIEM AVSSIRFAKS LGFNDIQFGC EDGGRSDKDF LCKILGEAIK
    260 270 280 290 300
    AGVTVVTIGD TVGINMPHEY GELVTYLKAN TPGIDDVVVA VHCHNDLGLA
    310 320 330 340 350
    TANSIAGIRA GARQVEVTIN GIGERSGNAS LEEVVMALKC RGAYVINGVY
    360 370 380 390 400
    TKIDTRQIMA TSKMVQEYTG LYVQAHKPIV GANCFVHESG IHQDGILKNR
    410 420 430 440 450
    STYEILSPED IGIVKSQNSG LVLGKLSGRH AVKDRLKELG YELDDEKLNA
    460 470 480 490 500
    VFSLFRDLTK NKKRITDADL KALVTSSDEI SLEKLNGANG LKSNGYIPVP

    QVSSNV
    Length:506
    Mass (Da):55,125
    Last modified:March 1, 2001 - v1
    Checksum:iA3F4CC32508CAE49
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti10V → G in strain: cv. Sorbo. 1
    Natural varianti42N → K in strain: cv. Ema-1 and cv. Pla-0. 1
    Natural varianti44A → T in strain: cv. Sorbo. 1
    Natural varianti505N → T in strain: cv. Sorbo. 1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ131517 Genomic DNA. Translation: CAC80102.1.
    AY049037 mRNA. Translation: AAL10687.1.
    AJ486882 mRNA. Translation: CAD31140.1.
    AJ486883 mRNA. Translation: CAD31141.1.
    AJ486884 mRNA. Translation: CAD31142.1.
    AJ486885 mRNA. Translation: CAD31143.1.
    AJ486886 mRNA. Translation: CAD31144.1.
    AJ486887 mRNA. Translation: CAD31145.1.
    AJ486888 mRNA. Translation: CAD31146.1.
    AM180572 Genomic DNA. Translation: CAJ55504.1.
    AB026660 Genomic DNA. Translation: BAB08874.1.
    CP002688 Genomic DNA. Translation: AED93107.1.
    AY054203 mRNA. Translation: AAL06864.1.
    AY070471 mRNA. Translation: AAL49937.1.
    AY149926 mRNA. Translation: AAN31080.1.
    RefSeqiNP_197692.1. NM_122207.3.
    UniGeneiAt.7567.

    Genome annotation databases

    EnsemblPlantsiAT5G23010.1; AT5G23010.1; AT5G23010.
    GeneIDi832365.
    GrameneiAT5G23010.1; AT5G23010.1; AT5G23010.
    KEGGiath:AT5G23010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ131517 Genomic DNA. Translation: CAC80102.1.
    AY049037 mRNA. Translation: AAL10687.1.
    AJ486882 mRNA. Translation: CAD31140.1.
    AJ486883 mRNA. Translation: CAD31141.1.
    AJ486884 mRNA. Translation: CAD31142.1.
    AJ486885 mRNA. Translation: CAD31143.1.
    AJ486886 mRNA. Translation: CAD31144.1.
    AJ486887 mRNA. Translation: CAD31145.1.
    AJ486888 mRNA. Translation: CAD31146.1.
    AM180572 Genomic DNA. Translation: CAJ55504.1.
    AB026660 Genomic DNA. Translation: BAB08874.1.
    CP002688 Genomic DNA. Translation: AED93107.1.
    AY054203 mRNA. Translation: AAL06864.1.
    AY070471 mRNA. Translation: AAL49937.1.
    AY149926 mRNA. Translation: AAN31080.1.
    RefSeqiNP_197692.1. NM_122207.3.
    UniGeneiAt.7567.

    3D structure databases

    ProteinModelPortaliQ9FG67.
    SMRiQ9FG67.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G23010.1.

    PTM databases

    iPTMnetiQ9FG67.

    Proteomic databases

    PaxDbiQ9FG67.
    PRIDEiQ9FG67.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G23010.1; AT5G23010.1; AT5G23010.
    GeneIDi832365.
    GrameneiAT5G23010.1; AT5G23010.1; AT5G23010.
    KEGGiath:AT5G23010.

    Organism-specific databases

    TAIRiAT5G23010.

    Phylogenomic databases

    eggNOGiKOG2367. Eukaryota.
    COG0119. LUCA.
    HOGENOMiHOG000046861.
    InParanoidiQ9FG67.
    KOiK15741.
    OMAiDTVGVMH.
    OrthoDBiEOG093608QE.
    PhylomeDBiQ9FG67.

    Enzyme and pathway databases

    BioCyciMetaCyc:AT5G23010-MONOMER.
    BRENDAi2.3.3.13. 399.

    Miscellaneous databases

    PROiQ9FG67.

    Gene expression databases

    GenevisibleiQ9FG67. AT.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    [Graphical view]
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMAM1_ARATH
    AccessioniPrimary (citable) accession number: Q9FG67
    Secondary accession number(s): Q70YX4, Q70YX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: November 2, 2016
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The 5'-part of the gene encoding this protein is deleted in cv. Bl-0, cv. Di-G, cv. Landsberg erecta and cv. Petergof, while the complete gene is missing in cv. Ka-0, cv. Lip-0, cv. No-0, cv. Sei-0, cv. Tsu-1 and cv. Wl-0.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.