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Protein

Probable protein phosphatase 2C 74

Gene

At5g36250

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Manganese 1By similarity1
Metal bindingi103Manganese 2By similarity1
Metal bindingi104Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi329Manganese 2By similarity1
Metal bindingi375Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • protein dephosphorylation Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 74 (EC:3.1.3.16)
Short name:
AtPP2C74
Gene namesi
Ordered Locus Names:At5g36250
ORF Names:T30G6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRilocus:2183612. AT5G36250.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
  • plasma membrane Source: TAIR

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679941 – 448Probable protein phosphatase 2C 74Add BLAST448

Proteomic databases

PaxDbiQ9FG61.

PTM databases

iPTMnetiQ9FG61.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FG61. baseline and differential.
GenevisibleiQ9FG61. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
At3g01090B9DFC12EBI-4437407,EBI-7801898

Protein-protein interaction databases

BioGridi18873. 1 interactor.
IntActiQ9FG61. 4 interactors.
MINTiMINT-8312718.
STRINGi3702.AT5G36250.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FG61.
SMRiQ9FG61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 384PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST318

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233897.
InParanoidiQ9FG61.
OMAiGHIVAKR.
OrthoDBiEOG09360CO1.
PhylomeDBiQ9FG61.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiView protein in Pfam
PF00481. PP2C. 1 hit.
SMARTiView protein in SMART
SM00332. PP2Cc. 1 hit.
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiView protein in PROSITE
PS51746. PPM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FG61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCLSSSGG GGSRRSLHGS PHVPGPGRRK RPPKRRPGSC SSSFDNTEEP
60 70 80 90 100
LLHRIPGRMF LNGSTDTVSL FSQQGKKGPN QDAMIVWENF GSMEDTVFCG
110 120 130 140 150
VFDGHGPYGH IVAKRVRDLL PLKLGSHLES YVSPEEVLKE ISLNTDDRKI
160 170 180 190 200
SEDLVHISAN GESRVYNKDY VKDQDMIQML IGSIVKAYRF MDKELKMQVD
210 220 230 240 250
VDCFCSGTTA VTMVKQGQHL VIGNIGDSRA VLGVRNKDNK LVPFQLTEDL
260 270 280 290 300
KPDVPAEAER IKRCRGRIFA LRDEPGVARL WLPNHNSPGL AMARAFGDFC
310 320 330 340 350
LKDFGLISVP DVSYRRLTEK DEFVVLATDG IWDALTNEEV VKIVAKAPTR
360 370 380 390 400
SSAGRALVEA AVRNWRWKFP TSKVDDCAVV CLFLDSEPNR LSTASFSKEK
410 420 430 440
HINNGVTEPE PDTASSSTPD SGTGSPELNG VNRIDTLVNL PVYVPTKE
Length:448
Mass (Da):49,314
Last modified:March 1, 2001 - v1
Checksum:iF60D6EA773B718C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57G → E in AAM47343 (PubMed:14593172).Curated1
Sequence conflicti57G → E in AAL31255 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026661 Genomic DNA. Translation: BAB09365.1.
CP002688 Genomic DNA. Translation: AED94062.1.
AY061752 mRNA. Translation: AAL31255.1.
AY113035 mRNA. Translation: AAM47343.1.
RefSeqiNP_198474.1. NM_123016.2.
UniGeneiAt.27370.

Genome annotation databases

EnsemblPlantsiAT5G36250.1; AT5G36250.1; AT5G36250.
GeneIDi833622.
GrameneiAT5G36250.1; AT5G36250.1; AT5G36250.
KEGGiath:AT5G36250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026661 Genomic DNA. Translation: BAB09365.1.
CP002688 Genomic DNA. Translation: AED94062.1.
AY061752 mRNA. Translation: AAL31255.1.
AY113035 mRNA. Translation: AAM47343.1.
RefSeqiNP_198474.1. NM_123016.2.
UniGeneiAt.27370.

3D structure databases

ProteinModelPortaliQ9FG61.
SMRiQ9FG61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18873. 1 interactor.
IntActiQ9FG61. 4 interactors.
MINTiMINT-8312718.
STRINGi3702.AT5G36250.1.

PTM databases

iPTMnetiQ9FG61.

Proteomic databases

PaxDbiQ9FG61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G36250.1; AT5G36250.1; AT5G36250.
GeneIDi833622.
GrameneiAT5G36250.1; AT5G36250.1; AT5G36250.
KEGGiath:AT5G36250.

Organism-specific databases

AraportiAT5G36250.
TAIRilocus:2183612. AT5G36250.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233897.
InParanoidiQ9FG61.
OMAiGHIVAKR.
OrthoDBiEOG09360CO1.
PhylomeDBiQ9FG61.

Enzyme and pathway databases

ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiQ9FG61.

Gene expression databases

ExpressionAtlasiQ9FG61. baseline and differential.
GenevisibleiQ9FG61. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiView protein in Pfam
PF00481. PP2C. 1 hit.
SMARTiView protein in SMART
SM00332. PP2Cc. 1 hit.
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiView protein in PROSITE
PS51746. PPM_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiP2C74_ARATH
AccessioniPrimary (citable) accession number: Q9FG61
Secondary accession number(s): Q8W4Q9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: March 15, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.