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Q9FG61

- P2C74_ARATH

UniProt

Q9FG61 - P2C74_ARATH

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Protein

Probable protein phosphatase 2C 74

Gene
At5g36250, T30G6.11
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Manganese 1 By similarity
Metal bindingi103 – 1031Manganese 2 By similarity
Metal bindingi104 – 1041Manganese 1; via carbonyl oxygen By similarity
Metal bindingi329 – 3291Manganese 2 By similarity
Metal bindingi375 – 3751Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: TAIR
  3. protein binding Source: IntAct

GO - Biological processi

  1. protein dephosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G36250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 74 (EC:3.1.3.16)
Short name:
AtPP2C74
Gene namesi
Ordered Locus Names:At5g36250
ORF Names:T30G6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G36250.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: TAIR
  2. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Probable protein phosphatase 2C 74PRO_0000367994Add
BLAST

Proteomic databases

PRIDEiQ9FG61.

Expressioni

Gene expression databases

GenevestigatoriQ9FG61.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
At3g01090B9DFC12EBI-4437407,EBI-7801898

Protein-protein interaction databases

BioGridi18873. 1 interaction.
IntActiQ9FG61. 4 interactions.
MINTiMINT-8312718.

Structurei

3D structure databases

ProteinModelPortaliQ9FG61.
SMRiQ9FG61. Positions 72-346.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 344139PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233897.
InParanoidiQ9FG61.
OMAiGHIVAKR.
PhylomeDBiQ9FG61.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9FG61-1 [UniParc]FASTAAdd to Basket

« Hide

MGSCLSSSGG GGSRRSLHGS PHVPGPGRRK RPPKRRPGSC SSSFDNTEEP    50
LLHRIPGRMF LNGSTDTVSL FSQQGKKGPN QDAMIVWENF GSMEDTVFCG 100
VFDGHGPYGH IVAKRVRDLL PLKLGSHLES YVSPEEVLKE ISLNTDDRKI 150
SEDLVHISAN GESRVYNKDY VKDQDMIQML IGSIVKAYRF MDKELKMQVD 200
VDCFCSGTTA VTMVKQGQHL VIGNIGDSRA VLGVRNKDNK LVPFQLTEDL 250
KPDVPAEAER IKRCRGRIFA LRDEPGVARL WLPNHNSPGL AMARAFGDFC 300
LKDFGLISVP DVSYRRLTEK DEFVVLATDG IWDALTNEEV VKIVAKAPTR 350
SSAGRALVEA AVRNWRWKFP TSKVDDCAVV CLFLDSEPNR LSTASFSKEK 400
HINNGVTEPE PDTASSSTPD SGTGSPELNG VNRIDTLVNL PVYVPTKE 448
Length:448
Mass (Da):49,314
Last modified:March 1, 2001 - v1
Checksum:iF60D6EA773B718C9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571G → E in AAM47343. 1 Publication
Sequence conflicti57 – 571G → E in AAL31255. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026661 Genomic DNA. Translation: BAB09365.1.
CP002688 Genomic DNA. Translation: AED94062.1.
AY061752 mRNA. Translation: AAL31255.1.
AY113035 mRNA. Translation: AAM47343.1.
RefSeqiNP_198474.1. NM_123016.1.
UniGeneiAt.27370.

Genome annotation databases

EnsemblPlantsiAT5G36250.1; AT5G36250.1; AT5G36250.
GeneIDi833622.
KEGGiath:AT5G36250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026661 Genomic DNA. Translation: BAB09365.1 .
CP002688 Genomic DNA. Translation: AED94062.1 .
AY061752 mRNA. Translation: AAL31255.1 .
AY113035 mRNA. Translation: AAM47343.1 .
RefSeqi NP_198474.1. NM_123016.1.
UniGenei At.27370.

3D structure databases

ProteinModelPortali Q9FG61.
SMRi Q9FG61. Positions 72-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18873. 1 interaction.
IntActi Q9FG61. 4 interactions.
MINTi MINT-8312718.

Proteomic databases

PRIDEi Q9FG61.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G36250.1 ; AT5G36250.1 ; AT5G36250 .
GeneIDi 833622.
KEGGi ath:AT5G36250.

Organism-specific databases

TAIRi AT5G36250.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000233897.
InParanoidi Q9FG61.
OMAi GHIVAKR.
PhylomeDBi Q9FG61.

Enzyme and pathway databases

BioCyci ARA:AT5G36250-MONOMER.

Gene expression databases

Genevestigatori Q9FG61.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C74_ARATH
AccessioniPrimary (citable) accession number: Q9FG61
Secondary accession number(s): Q8W4Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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