Q9FG61 (P2C74_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 74
Short name=AtPP2C74
EC=3.1.3.16

Gene names

Ordered Locus Names:	At5g36250
ORF Names:	T30G6.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from direct assay PubMed 22449965. Source: TAIR
Cellular_component	nucleus Inferred from direct assay PubMed 15610358. Source: TAIR plasma membrane Inferred from direct assay PubMed 22449965. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from direct assay PubMed 22449965. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Probable protein phosphatase 2C 74		PRO_0000367994
Regions
	Domain	206 – 344	139	PP2C-like
Sites
	Metal binding	103	1	Manganese 1 By similarity
	Metal binding	103	1	Manganese 2 By similarity
	Metal binding	104	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	329	1	Manganese 2 By similarity
	Metal binding	375	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	57	1	G → E in AAM47343. Ref.3
	Sequence conflict	57	1	G → E in AAL31255. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9FG61 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F60D6EA773B718C9	FASTA	448	49,314
	10 20 30 40 50 60 MGSCLSSSGG GGSRRSLHGS PHVPGPGRRK RPPKRRPGSC SSSFDNTEEP LLHRIPGRMF 70 80 90 100 110 120 LNGSTDTVSL FSQQGKKGPN QDAMIVWENF GSMEDTVFCG VFDGHGPYGH IVAKRVRDLL 130 140 150 160 170 180 PLKLGSHLES YVSPEEVLKE ISLNTDDRKI SEDLVHISAN GESRVYNKDY VKDQDMIQML 190 200 210 220 230 240 IGSIVKAYRF MDKELKMQVD VDCFCSGTTA VTMVKQGQHL VIGNIGDSRA VLGVRNKDNK 250 260 270 280 290 300 LVPFQLTEDL KPDVPAEAER IKRCRGRIFA LRDEPGVARL WLPNHNSPGL AMARAFGDFC 310 320 330 340 350 360 LKDFGLISVP DVSYRRLTEK DEFVVLATDG IWDALTNEEV VKIVAKAPTR SSAGRALVEA 370 380 390 400 410 420 AVRNWRWKFP TSKVDDCAVV CLFLDSEPNR LSTASFSKEK HINNGVTEPE PDTASSSTPD 430 440 SGTGSPELNG VNRIDTLVNL PVYVPTKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026661 Genomic DNA. Translation: BAB09365.1. CP002688 Genomic DNA. Translation: AED94062.1. AY061752 mRNA. Translation: AAL31255.1. AY113035 mRNA. Translation: AAM47343.1.
IPI	IPI00538708.
RefSeq	NP_198474.1. NM_123016.1.
UniGene	At.27370.
3D structure databases
ProteinModelPortal	Q9FG61.
SMR	Q9FG61. Positions 72-346.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FG61. 3 interactions.
Proteomic databases
PRIDE	Q9FG61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G36250.1; AT5G36250.1; AT5G36250.
GeneID	833622.
KEGG	ath:AT5G36250.
Organism-specific databases
TAIR	At5g36250.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233897.
InParanoid	Q9FG61.
OMA	GHIVAKR.
PhylomeDB	Q9FG61.
ProtClustDB	CLSN2685212.
Gene expression databases
Genevestigator	Q9FG61.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C74_ARATH

Accession

Primary (citable) accession number: Q9FG61
Secondary accession number(s): Q8W4Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program