Q9FG59 (DHAR4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glutathione S-transferase DHAR4
EC=2.5.1.18

Alternative name(s):
Chloride intracellular channel homolog 4
Short name=CLIC homolog 4
Glutathione-dependent dehydroascorbate reductase 4
Short name=AtDHAR4
Short name=GSH-dependent dehydroascorbate reductase 4

Gene names

Name:	DHAR4
Ordered Locus Names:	At5g36270
ORF Names:	T30G6.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm › cytosol Potential.
Sequence similarities	Belongs to the GST superfamily. DHAR family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Caution	Could be the product of a pseudogene.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Putative glutathione S-transferase DHAR4		PRO_0000395484
Regions
	Domain	10 – 85	76	GST N-terminal
	Domain	86 – 217	132	GST C-terminal
	Region	20 – 21	2	Glutathione binding By similarity
	Region	46 – 47	2	Glutathione binding By similarity
	Region	59 – 60	2	Glutathione binding By similarity
	Region	74 – 75	2	Glutathione binding By similarity
	Motif	20 – 25	6	Glutathione-binding Potential
Sites
	Active site	20	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FG59 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FA21044464FDB0EA	FASTA	217	24,060
	10 20 30 40 50 60 MGIEVCVKAA SGAPDVLGDC PFGQRILLTL EDKKLPYKTH LIDVSLKPDW FLAISPKGKL 70 80 90 100 110 120 PLVKFDEDEN WVADSDLIVG IIEEKYPEPS LVTFPPEFAS VGSKIIGAFV MFLTSKDHAN 130 140 150 160 170 180 DGSDMALLDE LEALDHHLKT HVGPFVAGDK VTVVDLSLAP KLYHLETTLG HFMDWCVPES 190 200 210 LTNVRDYMKV LFSLESFEKT KAAKEYLIAS WAPKLDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional divergence in the glutathione transferase superfamily in plants. Identification of two classes with putative functions in redox homeostasis in Arabidopsis thaliana." Dixon D.P., Davis B.G., Edwards R. J. Biol. Chem. 277:30859-30869(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026661 Genomic DNA. Translation: BAB09367.1.
IPI	IPI00527407.
3D structure databases
ProteinModelPortal	Q9FG59.
SMR	Q9FG59. Positions 20-184.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
TAIR	At5g36270.
Gene expression databases
Genevestigator	Q9FG59.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAR4_ARATH

Accession

Primary (citable) accession number: Q9FG59

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program