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Q9FG34 (PER54_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxidase 54
Short name=Atperox P54
EC=1.11.1.7
Alternative name(s):
ATP29a
Gene names
Name:PER54
Synonyms:P54
Ordered Locus Names:At5g06730
ORF Names:MPH15.9
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activity

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Subcellular location

Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Sequence similarities

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Ontologies

Keywords
   Biological processHydrogen peroxide
   Cellular componentSecreted
Vacuole
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
Peroxidase
   PTMDisulfide bond
Glycoprotein
Pyrrolidone carboxylic acid
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processhydrogen peroxide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

vacuolar membrane

Inferred from direct assay. Source: TAIR

   Molecular functionheme binding

Inferred from electronic annotation. Source: InterPro

peroxidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 358327Peroxidase 54
PRO_0000023719

Sites

Active site731Proton acceptor By similarity
Metal binding741Calcium 1 By similarity
Metal binding771Calcium 1; via carbonyl oxygen By similarity
Metal binding791Calcium 1; via carbonyl oxygen By similarity
Metal binding811Calcium 1 By similarity
Metal binding831Calcium 1 By similarity
Metal binding2001Iron (heme axial ligand) By similarity
Metal binding2011Calcium 2 By similarity
Metal binding2521Calcium 2 By similarity
Metal binding2551Calcium 2 By similarity
Metal binding2601Calcium 2 By similarity
Binding site1701Substrate; via carbonyl oxygen By similarity
Site691Transition state stabilizer By similarity

Amino acid modifications

Modified residue321Pyrrolidone carboxylic acid By similarity
Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2181N-linked (GlcNAc...) Potential
Glycosylation2281N-linked (GlcNAc...) Potential
Glycosylation2421N-linked (GlcNAc...) Potential
Glycosylation2981N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 122 By similarity
Disulfide bond75 ↔ 80 By similarity
Disulfide bond128 ↔ 330 By similarity
Disulfide bond207 ↔ 239 By similarity

Experimental info

Sequence conflict211I → V in AAM66044. Ref.5
Sequence conflict281T → A in AAM66044. Ref.5
Sequence conflict1891K → N in AAM66044. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9FG34 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F9841562BAC5106B

FASTA35837,290
        10         20         30         40         50         60 
MAVTSSSSTC DGFFIISLIV IVSSLFGTSS AQLNATFYSG TCPNASAIVR STIQQALQSD 

        70         80         90        100        110        120 
ARIGGSLIRL HFHDCFVNGC DGSLLLDDTS SIQSEKNAPA NANSTRGFNV VDSIKTALEN 

       130        140        150        160        170        180 
ACPGIVSCSD ILALASEASV SLAGGPSWTV LLGRRDGLTA NLSGANSSLP SPFEGLNNIT 

       190        200        210        220        230        240 
SKFVAVGLKT TDVVSLSGAH TFGRGQCVTF NNRLFNFNGT GNPDPTLNST LLSSLQQLCP 

       250        260        270        280        290        300 
QNGSNTGITN LDLSTPDAFD NNYFTNLQSN NGLLQSDQEL FSNTGSATVP IVNSFASNQT 

       310        320        330        340        350 
LFFEAFVQSM IKMGNISPLT GSSGEIRQDC KVVNGQSSAT EAGDIQLQSD GPVSVADM

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"From expressed sequence tags to structure, function, evolution and expression of 28 ER-targeted Arabidopsis peroxidases."
Welinder K.G., Jespersen H.M., Kjaersgaard I.V.H., Justesen A.F., Oestergaard L., Abelskov A.K., Jensen R.B., Hansen L.N., Rasmussen S.K.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 212-358.
Strain: cv. Columbia.
Tissue: Root.
[7]"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
Tognolli M., Penel C., Greppin H., Simon P.
Gene 288:129-138(2002) [PubMed: 12034502] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP002032 Genomic DNA. Translation: BAB09807.1.
CP002688 Genomic DNA. Translation: AED91056.1.
AK118827 mRNA. Translation: BAC43417.1.
BT008584 mRNA. Translation: AAP40411.1.
AY088509 mRNA. Translation: AAM66044.1.
Y11794 mRNA. Translation: CAA72490.1.
IPIIPI00528868.
RefSeqNP_196291.1. NM_120756.2.
UniGeneAt.90.

3D structure databases

ProteinModelPortalQ9FG34.
SMRQ9FG34. Positions 32-335.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9FG34.

Protein family/group databases

PeroxiBase220. AtPrx54.

Proteomic databases

PRIDEQ9FG34.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G06730.1; AT5G06730.1; AT5G06730.
GeneID830562.
GenomeReviewsGene locus AT5G06730 in contig BA000015_GR.
KEGGath:AT5G06730.
NMPDRfig|3702.1.peg.22804.

Organism-specific databases

GeneFarm1908. 61.
TAIRAt5g06730.

Phylogenomic databases

GeneTreeEPGT00070000028241.
HOGENOMHBG597790.
InParanoidQ9FG34.
PhylomeDBQ9FG34.
ProtClustDBCLSN2686891.

Gene expression databases

ArrayExpressQ9FG34.
GenevestigatorQ9FG34.
GermOnlineAT5G06730. Arabidopsis thaliana.

Family and domain databases

InterProIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
KOK00430.
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMSSF48113. Peroxidase_super. 1 hit.
PROSITEPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePER54_ARATH
AccessionPrimary (citable) accession number: Q9FG34
Secondary accession number(s): P93729
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: March 1, 2001
Last modified: December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents