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Protein

Serine/threonine-protein kinase SRK2H

Gene

SRK2H

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei123 – 1231Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • protein phosphorylation Source: GO_Central
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G63650-MONOMER.
ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SRK2H (EC:2.7.11.1)
Alternative name(s):
OST1-kinase-like 9
SNF1-related kinase 2.5
Short name:
SnRK2.5
Gene namesi
Name:SRK2H
Synonyms:OSKL9, SNRK2.5
Ordered Locus Names:At5g63650
ORF Names:MBK5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G63650.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Serine/threonine-protein kinase SRK2HPRO_0000345162Add
BLAST

Proteomic databases

PaxDbiQ9FFP9.
PRIDEiQ9FFP9.

PTM databases

iPTMnetiQ9FFP9.

Expressioni

Tissue specificityi

Expressed in seedlings.1 Publication

Inductioni

By salt and osmotic stress (at protein level).1 Publication

Gene expression databases

GenevisibleiQ9FFP9. AT.

Interactioni

Protein-protein interaction databases

BioGridi21727. 2 interactions.
STRINGi3702.AT5G63650.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FFP9.
SMRiQ9FFP9. Positions 1-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 260257Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi316 – 35035Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ9FFP9.
KOiK14498.
OMAiAYYKRET.
PhylomeDBiQ9FFP9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FFP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKYEVVKDL GAGNFGVARL LRHKETKELV AMKYIERGRK IDENVAREII
60 70 80 90 100
NHRSLRHPNI IRFKEVILTP THLAIVMEYA SGGELFERIC NAGRFSEAEA
110 120 130 140 150
RYFFQQLICG VDYCHSLQIC HRDLKLENTL LDGSPAPLLK ICDFGYSKSS
160 170 180 190 200
LLHSRPKSTV GTPAYIAPEV LSRREYDGKH ADVWSCGVTL YVMLVGGYPF
210 220 230 240 250
EDPDDPRNFR KTIQRIMAVQ YKIPDYVHIS QECRHLLSRI FVTNSAKRIT
260 270 280 290 300
LKEIKKHPWY LKNLPKELTE PAQAAYYKRE TPSFSLQSVE DIMKIVGEAR
310 320 330 340 350
NPAPSSNAVK GFDDDEEDVE DEVEEEEEEE EEEEEEEEEE EDEYEKHVKE
360
AHSCQEPPKA
Length:360
Mass (Da):41,554
Last modified:March 1, 2001 - v1
Checksum:iD4ECB111E954A5A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741R → K in BAE99712 (Ref. 3) Curated
Sequence conflicti335 – 3351E → V in BAE99712 (Ref. 3) Curated
Sequence conflicti340 – 3412Missing in AAM65503 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005234 Genomic DNA. Translation: BAB10458.1.
CP002688 Genomic DNA. Translation: AED97781.1.
AK227726 mRNA. Translation: BAE99712.1.
AY087955 mRNA. Translation: AAM65503.1.
RefSeqiNP_201170.1. NM_125760.1.
UniGeneiAt.28988.

Genome annotation databases

EnsemblPlantsiAT5G63650.1; AT5G63650.1; AT5G63650.
GeneIDi836485.
GrameneiAT5G63650.1; AT5G63650.1; AT5G63650.
KEGGiath:AT5G63650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005234 Genomic DNA. Translation: BAB10458.1.
CP002688 Genomic DNA. Translation: AED97781.1.
AK227726 mRNA. Translation: BAE99712.1.
AY087955 mRNA. Translation: AAM65503.1.
RefSeqiNP_201170.1. NM_125760.1.
UniGeneiAt.28988.

3D structure databases

ProteinModelPortaliQ9FFP9.
SMRiQ9FFP9. Positions 1-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21727. 2 interactions.
STRINGi3702.AT5G63650.1.

PTM databases

iPTMnetiQ9FFP9.

Proteomic databases

PaxDbiQ9FFP9.
PRIDEiQ9FFP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G63650.1; AT5G63650.1; AT5G63650.
GeneIDi836485.
GrameneiAT5G63650.1; AT5G63650.1; AT5G63650.
KEGGiath:AT5G63650.

Organism-specific databases

TAIRiAT5G63650.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ9FFP9.
KOiK14498.
OMAiAYYKRET.
PhylomeDBiQ9FFP9.

Enzyme and pathway databases

BioCyciARA:AT5G63650-MONOMER.
ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

PROiQ9FFP9.

Gene expression databases

GenevisibleiQ9FFP9. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana."
    Boudsocq M., Barbier-Brygoo H., Lauriere C.
    J. Biol. Chem. 279:41758-41766(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  7. "The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis."
    Yoshida R., Umezawa T., Mizoguchi T., Takahashi S., Takahashi F., Shinozaki K.
    J. Biol. Chem. 281:5310-5318(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiSRK2H_ARATH
AccessioniPrimary (citable) accession number: Q9FFP9
Secondary accession number(s): Q0WT36, Q8LA99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.