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Q9FFN7

- DGK2_ARATH

UniProt

Q9FFN7 - DGK2_ARATH

Protein

Diacylglycerol kinase 2

Gene

DGK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress. Exhibits high specificity for 1-stearoyl-2-arachidonoyl- sn-glycerol (1,2-SAG), 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG), but has almost no activity toward 1,2-dioctanoyl-sn-glycerol (1,2-DOCG), 1,2-dipalmitoyl-sn-glycerol (1,2-DPG), 1,2-dimyristoyl-sn-glycerol (1,2-DMG) and 1-oleoyl-2-acetyl-sn-glycerol (1,2-OAG).3 Publications

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

    Kineticsi

    1. KM=125 µM for 1,2-dioleoyl-sn-glycerol1 Publication

    Vmax=0.25 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol1 Publication

    pH dependencei

    Optimum pH is 7.2.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 13362Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri145 – 20864Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: TAIR
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. intracellular signal transduction Source: InterPro
    3. leaf development Source: TAIR
    4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    5. response to cold Source: TAIR
    6. response to wounding Source: TAIR
    7. root development Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Plant defense, Stress response

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT5G63770-MONOMER.
    ARA:GQT-1857-MONOMER.
    MetaCyc:AT5G63770-MONOMER.
    BRENDAi2.7.1.107. 302.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase 2 (EC:2.7.1.107)
    Short name:
    AtDGK2
    Short name:
    DAG kinase 2
    Alternative name(s):
    Diglyceride kinase 2
    Short name:
    DGK 2
    Gene namesi
    Name:DGK2
    Ordered Locus Names:At5g63770
    ORF Names:MBK5.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G63770.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 712712Diacylglycerol kinase 2PRO_0000422110Add
    BLAST

    Proteomic databases

    PRIDEiQ9FFN7.

    Expressioni

    Tissue specificityi

    Expressed in rosette and cauline leaves, flowers, siliques and roots. Highly expressed in young leaves and at lower levels in older leaves. In young seedlings, expressed at the root-shoot junction zone and vascular bundles of the cotyledons. In older plants, expressed in root tip, central cylinder, root hair, leaf mesophyll cells and guard cells, sepals, filaments of the anthers, stigma, valves of young and early adult siliques and hilum of seeds.1 Publication

    Inductioni

    By cold stress and wounding.2 Publications

    Gene expression databases

    GenevestigatoriQ9FFN7.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    IntActiQ9FFN7. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FFN7.
    SMRiQ9FFN7. Positions 71-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini338 – 479142DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 13362Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri145 – 20864Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    HOGENOMiHOG000005923.
    InParanoidiQ9FFN7.
    KOiK00901.
    OMAiCMKTSLK.
    PhylomeDBiQ9FFN7.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FFN7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMEVGFSLIQ WLISSGADSP FIFGWLVTGS VGLLAVIYTF LKWQKKTSLN    50
    WVKAAAREKK KVWKRLRVPL SHHQWTDDYG YGQQPSTCCV CLYSLVPGQN 100
    VSNKASLSIP VHRCAVCGVA AHFYCSSSAA KDCKCVAQAG SDHVRHHWSE 150
    RWVNMDDNAD MTAFCFYCDE PCGIPFIEAS PMWHCLWCQR LIHVKCHMIM 200
    SKESGDACDL GSLRRVILSP VHVKLNEANG VDGVLTTIKN ELASIRGHVR 250
    RKRHRGKNGN GQSLNGKLLE DSVSDPVKTV VNGLVVKKLR RDRSIDCLKQ 300
    VSDMPNAKGL QNGIGGHKRN KSAALNFMKK FSLVDLPPDA RPLLVFINAK 350
    SGGQLGPFLH RRLNMLLNPV QVFELGSCQG PDAGLDLCSK VKYFRVLVCG 400
    GDGTVAWVLD AIEKRNFESP PPVAILPLGT GNDLSRVLQW GRGISVVDGQ 450
    GSLRTFLQDI DHAAVTMLDR WSVKIVEEST EKFPAREGHK FMMNYLGIGC 500
    DAKVAYEFHM MRQEKPEKFC SQFVNKLRYA KEGARDIMDR ACADLPWQVW 550
    LEVDGKDIEI PKDSEGLIVL NIGSYMGGVD LWQNDYEHDD NFSIQCMHDK 600
    TLEVVCVRGA WHLGKLQVGL SQARRLAQGK VIRIHVSSPF PVQIDGEPFI 650
    QQPGCLEITH HGQVFMLRRA SDEPRGHAAA IMNEVLLDAE CKGVINASQK 700
    KVLLQQMALH LS 712
    Length:712
    Mass (Da):79,405
    Last modified:March 1, 2001 - v1
    Checksum:iE1416E8B22836F3E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY380783 mRNA. Translation: AAR28755.1.
    AB005234 Genomic DNA. Translation: BAB10470.1.
    CP002688 Genomic DNA. Translation: AED97795.1.
    AY062655 mRNA. Translation: AAL32733.1.
    BT008792 mRNA. Translation: AAP68231.1.
    AK228735 mRNA. Translation: BAF00636.1.
    RefSeqiNP_201182.1. NM_125772.4. [Q9FFN7-1]
    UniGeneiAt.8705.

    Genome annotation databases

    EnsemblPlantsiAT5G63770.1; AT5G63770.1; AT5G63770. [Q9FFN7-1]
    GeneIDi836497.
    KEGGiath:AT5G63770.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY380783 mRNA. Translation: AAR28755.1 .
    AB005234 Genomic DNA. Translation: BAB10470.1 .
    CP002688 Genomic DNA. Translation: AED97795.1 .
    AY062655 mRNA. Translation: AAL32733.1 .
    BT008792 mRNA. Translation: AAP68231.1 .
    AK228735 mRNA. Translation: BAF00636.1 .
    RefSeqi NP_201182.1. NM_125772.4. [Q9FFN7-1 ]
    UniGenei At.8705.

    3D structure databases

    ProteinModelPortali Q9FFN7.
    SMRi Q9FFN7. Positions 71-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9FFN7. 2 interactions.

    Proteomic databases

    PRIDEi Q9FFN7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G63770.1 ; AT5G63770.1 ; AT5G63770 . [Q9FFN7-1 ]
    GeneIDi 836497.
    KEGGi ath:AT5G63770.

    Organism-specific databases

    TAIRi AT5G63770.

    Phylogenomic databases

    HOGENOMi HOG000005923.
    InParanoidi Q9FFN7.
    KOi K00901.
    OMAi CMKTSLK.
    PhylomeDBi Q9FFN7.

    Enzyme and pathway databases

    BioCyci ARA:AT5G63770-MONOMER.
    ARA:GQT-1857-MONOMER.
    MetaCyc:AT5G63770-MONOMER.
    BRENDAi 2.7.1.107. 302.

    Miscellaneous databases

    PROi Q9FFN7.

    Gene expression databases

    Genevestigatori Q9FFN7.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression."
      Gomez-Merino F.C., Brearley C.A., Ornatowska M., Abdel-Haliem M.E., Zanor M.I., Mueller-Roeber B.
      J. Biol. Chem. 279:8230-8241(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION BY COLD.
      Strain: cv. C24.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
      Gomez-Merino F.C., Arana-Ceballos F.A., Trejo-Tellez L.I., Skirycz A., Brearley C.A., Doermann P., Mueller-Roeber B.
      J. Biol. Chem. 280:34888-34899(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY WOUNDING.
    7. "Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase."
      Arisz S.A., van Wijk R., Roels W., Zhu J.K., Haring M.A., Munnik T.
      Front. Plant Sci. 4:1-1(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDGK2_ARATH
    AccessioniPrimary (citable) accession number: Q9FFN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3