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Q9FFN7

- DGK2_ARATH

UniProt

Q9FFN7 - DGK2_ARATH

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Protein

Diacylglycerol kinase 2

Gene
DGK2, At5g63770, MBK5.25
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress. Exhibits high specificity for 1-stearoyl-2-arachidonoyl- sn-glycerol (1,2-SAG), 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG), but has almost no activity toward 1,2-dioctanoyl-sn-glycerol (1,2-DOCG), 1,2-dipalmitoyl-sn-glycerol (1,2-DPG), 1,2-dimyristoyl-sn-glycerol (1,2-DMG) and 1-oleoyl-2-acetyl-sn-glycerol (1,2-OAG).3 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Kineticsi

  1. KM=125 µM for 1,2-dioleoyl-sn-glycerol1 Publication

Vmax=0.25 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol

pH dependencei

Optimum pH is 7.2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 13362Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri145 – 20864Phorbol-ester/DAG-type 2Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: TAIR
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. intracellular signal transduction Source: InterPro
  3. leaf development Source: TAIR
  4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  5. response to cold Source: TAIR
  6. response to wounding Source: TAIR
  7. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G63770-MONOMER.
ARA:GQT-1857-MONOMER.
MetaCyc:AT5G63770-MONOMER.
BRENDAi2.7.1.107. 302.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 2 (EC:2.7.1.107)
Short name:
AtDGK2
Short name:
DAG kinase 2
Alternative name(s):
Diglyceride kinase 2
Short name:
DGK 2
Gene namesi
Name:DGK2
Ordered Locus Names:At5g63770
ORF Names:MBK5.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G63770.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712Diacylglycerol kinase 2PRO_0000422110Add
BLAST

Proteomic databases

PRIDEiQ9FFN7.

Expressioni

Tissue specificityi

Expressed in rosette and cauline leaves, flowers, siliques and roots. Highly expressed in young leaves and at lower levels in older leaves. In young seedlings, expressed at the root-shoot junction zone and vascular bundles of the cotyledons. In older plants, expressed in root tip, central cylinder, root hair, leaf mesophyll cells and guard cells, sepals, filaments of the anthers, stigma, valves of young and early adult siliques and hilum of seeds.1 Publication

Inductioni

By cold stress and wounding.2 Publications

Gene expression databases

GenevestigatoriQ9FFN7.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

IntActiQ9FFN7. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9FFN7.
SMRiQ9FFN7. Positions 71-213.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini338 – 479142DAGKcAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 13362Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri145 – 20864Phorbol-ester/DAG-type 2Add
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000005923.
InParanoidiQ9FFN7.
KOiK00901.
OMAiCMKTSLK.
PhylomeDBiQ9FFN7.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FFN7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMEVGFSLIQ WLISSGADSP FIFGWLVTGS VGLLAVIYTF LKWQKKTSLN    50
WVKAAAREKK KVWKRLRVPL SHHQWTDDYG YGQQPSTCCV CLYSLVPGQN 100
VSNKASLSIP VHRCAVCGVA AHFYCSSSAA KDCKCVAQAG SDHVRHHWSE 150
RWVNMDDNAD MTAFCFYCDE PCGIPFIEAS PMWHCLWCQR LIHVKCHMIM 200
SKESGDACDL GSLRRVILSP VHVKLNEANG VDGVLTTIKN ELASIRGHVR 250
RKRHRGKNGN GQSLNGKLLE DSVSDPVKTV VNGLVVKKLR RDRSIDCLKQ 300
VSDMPNAKGL QNGIGGHKRN KSAALNFMKK FSLVDLPPDA RPLLVFINAK 350
SGGQLGPFLH RRLNMLLNPV QVFELGSCQG PDAGLDLCSK VKYFRVLVCG 400
GDGTVAWVLD AIEKRNFESP PPVAILPLGT GNDLSRVLQW GRGISVVDGQ 450
GSLRTFLQDI DHAAVTMLDR WSVKIVEEST EKFPAREGHK FMMNYLGIGC 500
DAKVAYEFHM MRQEKPEKFC SQFVNKLRYA KEGARDIMDR ACADLPWQVW 550
LEVDGKDIEI PKDSEGLIVL NIGSYMGGVD LWQNDYEHDD NFSIQCMHDK 600
TLEVVCVRGA WHLGKLQVGL SQARRLAQGK VIRIHVSSPF PVQIDGEPFI 650
QQPGCLEITH HGQVFMLRRA SDEPRGHAAA IMNEVLLDAE CKGVINASQK 700
KVLLQQMALH LS 712
Length:712
Mass (Da):79,405
Last modified:March 1, 2001 - v1
Checksum:iE1416E8B22836F3E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY380783 mRNA. Translation: AAR28755.1.
AB005234 Genomic DNA. Translation: BAB10470.1.
CP002688 Genomic DNA. Translation: AED97795.1.
AY062655 mRNA. Translation: AAL32733.1.
BT008792 mRNA. Translation: AAP68231.1.
AK228735 mRNA. Translation: BAF00636.1.
RefSeqiNP_201182.1. NM_125772.4. [Q9FFN7-1]
UniGeneiAt.8705.

Genome annotation databases

EnsemblPlantsiAT5G63770.1; AT5G63770.1; AT5G63770. [Q9FFN7-1]
GeneIDi836497.
KEGGiath:AT5G63770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY380783 mRNA. Translation: AAR28755.1 .
AB005234 Genomic DNA. Translation: BAB10470.1 .
CP002688 Genomic DNA. Translation: AED97795.1 .
AY062655 mRNA. Translation: AAL32733.1 .
BT008792 mRNA. Translation: AAP68231.1 .
AK228735 mRNA. Translation: BAF00636.1 .
RefSeqi NP_201182.1. NM_125772.4. [Q9FFN7-1 ]
UniGenei At.8705.

3D structure databases

ProteinModelPortali Q9FFN7.
SMRi Q9FFN7. Positions 71-213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9FFN7. 2 interactions.

Proteomic databases

PRIDEi Q9FFN7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G63770.1 ; AT5G63770.1 ; AT5G63770 . [Q9FFN7-1 ]
GeneIDi 836497.
KEGGi ath:AT5G63770.

Organism-specific databases

TAIRi AT5G63770.

Phylogenomic databases

HOGENOMi HOG000005923.
InParanoidi Q9FFN7.
KOi K00901.
OMAi CMKTSLK.
PhylomeDBi Q9FFN7.

Enzyme and pathway databases

BioCyci ARA:AT5G63770-MONOMER.
ARA:GQT-1857-MONOMER.
MetaCyc:AT5G63770-MONOMER.
BRENDAi 2.7.1.107. 302.

Miscellaneous databases

PROi Q9FFN7.

Gene expression databases

Genevestigatori Q9FFN7.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression."
    Gomez-Merino F.C., Brearley C.A., Ornatowska M., Abdel-Haliem M.E., Zanor M.I., Mueller-Roeber B.
    J. Biol. Chem. 279:8230-8241(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION BY COLD.
    Strain: cv. C24.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
    Gomez-Merino F.C., Arana-Ceballos F.A., Trejo-Tellez L.I., Skirycz A., Brearley C.A., Doermann P., Mueller-Roeber B.
    J. Biol. Chem. 280:34888-34899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY WOUNDING.
  7. "Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase."
    Arisz S.A., van Wijk R., Roels W., Zhu J.K., Haring M.A., Munnik T.
    Front. Plant Sci. 4:1-1(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDGK2_ARATH
AccessioniPrimary (citable) accession number: Q9FFN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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