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Protein

Diacylglycerol kinase 2

Gene

DGK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress. Exhibits high specificity for 1-stearoyl-2-arachidonoyl- sn-glycerol (1,2-SAG), 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG), but has almost no activity toward 1,2-dioctanoyl-sn-glycerol (1,2-DOCG), 1,2-dipalmitoyl-sn-glycerol (1,2-DPG), 1,2-dimyristoyl-sn-glycerol (1,2-DMG) and 1-oleoyl-2-acetyl-sn-glycerol (1,2-OAG).3 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Kineticsi

  1. KM=125 µM for 1,2-dioleoyl-sn-glycerol1 Publication
  1. Vmax=0.25 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol1 Publication

pH dependencei

Optimum pH is 7.2.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 133Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST62
Zinc fingeri145 – 208Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST64

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • intracellular signal transduction Source: InterPro
  • leaf development Source: TAIR
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • response to cold Source: TAIR
  • response to wounding Source: TAIR
  • root development Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
Biological processPlant defense, Stress response
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G63770-MONOMER
MetaCyc:AT5G63770-MONOMER
BRENDAi2.7.1.107 399
ReactomeiR-ATH-114508 Effects of PIP2 hydrolysis

Chemistry databases

SwissLipidsiSLP:000001675

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 2 (EC:2.7.1.107)
Short name:
AtDGK2
Short name:
DAG kinase 2
Alternative name(s):
Diglyceride kinase 2
Short name:
DGK 2
Gene namesi
Name:DGK2
Ordered Locus Names:At5g63770
ORF Names:MBK5.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G63770
TAIRilocus:2160604 AT5G63770

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221101 – 712Diacylglycerol kinase 2Add BLAST712

Proteomic databases

PaxDbiQ9FFN7

PTM databases

iPTMnetiQ9FFN7

Expressioni

Tissue specificityi

Expressed in rosette and cauline leaves, flowers, siliques and roots. Highly expressed in young leaves and at lower levels in older leaves. In young seedlings, expressed at the root-shoot junction zone and vascular bundles of the cotyledons. In older plants, expressed in root tip, central cylinder, root hair, leaf mesophyll cells and guard cells, sepals, filaments of the anthers, stigma, valves of young and early adult siliques and hilum of seeds.1 Publication

Inductioni

By cold stress and wounding.2 Publications

Gene expression databases

ExpressionAtlasiQ9FFN7 baseline and differential
GenevisibleiQ9FFN7 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi21739, 1 interactor
IntActiQ9FFN7, 2 interactors
STRINGi3702.AT5G63770.1

Structurei

3D structure databases

ProteinModelPortaliQ9FFN7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini338 – 479DAGKcPROSITE-ProRule annotationAdd BLAST142

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 133Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST62
Zinc fingeri145 – 208Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST64

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
HOGENOMiHOG000005923
InParanoidiQ9FFN7
KOiK00901
OMAiAHTVRLI
OrthoDBiEOG09360446
PhylomeDBiQ9FFN7

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FFN7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEVGFSLIQ WLISSGADSP FIFGWLVTGS VGLLAVIYTF LKWQKKTSLN
60 70 80 90 100
WVKAAAREKK KVWKRLRVPL SHHQWTDDYG YGQQPSTCCV CLYSLVPGQN
110 120 130 140 150
VSNKASLSIP VHRCAVCGVA AHFYCSSSAA KDCKCVAQAG SDHVRHHWSE
160 170 180 190 200
RWVNMDDNAD MTAFCFYCDE PCGIPFIEAS PMWHCLWCQR LIHVKCHMIM
210 220 230 240 250
SKESGDACDL GSLRRVILSP VHVKLNEANG VDGVLTTIKN ELASIRGHVR
260 270 280 290 300
RKRHRGKNGN GQSLNGKLLE DSVSDPVKTV VNGLVVKKLR RDRSIDCLKQ
310 320 330 340 350
VSDMPNAKGL QNGIGGHKRN KSAALNFMKK FSLVDLPPDA RPLLVFINAK
360 370 380 390 400
SGGQLGPFLH RRLNMLLNPV QVFELGSCQG PDAGLDLCSK VKYFRVLVCG
410 420 430 440 450
GDGTVAWVLD AIEKRNFESP PPVAILPLGT GNDLSRVLQW GRGISVVDGQ
460 470 480 490 500
GSLRTFLQDI DHAAVTMLDR WSVKIVEEST EKFPAREGHK FMMNYLGIGC
510 520 530 540 550
DAKVAYEFHM MRQEKPEKFC SQFVNKLRYA KEGARDIMDR ACADLPWQVW
560 570 580 590 600
LEVDGKDIEI PKDSEGLIVL NIGSYMGGVD LWQNDYEHDD NFSIQCMHDK
610 620 630 640 650
TLEVVCVRGA WHLGKLQVGL SQARRLAQGK VIRIHVSSPF PVQIDGEPFI
660 670 680 690 700
QQPGCLEITH HGQVFMLRRA SDEPRGHAAA IMNEVLLDAE CKGVINASQK
710
KVLLQQMALH LS
Length:712
Mass (Da):79,405
Last modified:March 1, 2001 - v1
Checksum:iE1416E8B22836F3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY380783 mRNA Translation: AAR28755.1
AB005234 Genomic DNA Translation: BAB10470.1
CP002688 Genomic DNA Translation: AED97795.1
CP002688 Genomic DNA Translation: AED97796.2
AY062655 mRNA Translation: AAL32733.1
BT008792 mRNA Translation: AAP68231.1
AK228735 mRNA Translation: BAF00636.1
RefSeqiNP_001318872.1, NM_001345610.1 [Q9FFN7-1]
NP_201182.1, NM_125772.5 [Q9FFN7-1]
UniGeneiAt.8705

Genome annotation databases

EnsemblPlantsiAT5G63770.1; AT5G63770.1; AT5G63770 [Q9FFN7-1]
AT5G63770.2; AT5G63770.2; AT5G63770 [Q9FFN7-1]
GeneIDi836497
GrameneiAT5G63770.1; AT5G63770.1; AT5G63770 [Q9FFN7-1]
AT5G63770.2; AT5G63770.2; AT5G63770 [Q9FFN7-1]
KEGGiath:AT5G63770

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDGK2_ARATH
AccessioniPrimary (citable) accession number: Q9FFN7
Secondary accession number(s): A8MRH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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