Reviewed, UniProtKB/Swiss-Prot Q9FFN4 (BGAL6_ARATH)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase 6
      Short name=Lactase 6
    EC=3.2.1.23

Gene names

Name:	BGAL6
Ordered Locus Names:	At5g63800
ORF Names:	MBK5.28

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Tissue specificity	Expressed at low levels in siliques. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.
Sequence caution	The sequence CAB64742.1 differs from that shown. Reason: Frameshift at positions 453 and 457.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro mucilage biosynthetic process during seed coat development Inferred from mutant phenotype. Source: TAIR plant-type cell wall modification Inferred from mutant phenotype. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR cell wall Inferred from direct assay. Source: TAIR
Molecular function	beta-galactosidase activity Inferred from direct assay. Source: TAIR cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 718	690	Beta-galactosidase 6		PRO_5000065881
Sites
	Active site	188	1	Proton donor Potential
	Active site	259	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential
	Glycosylation	538	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	70	1	T → A in BAF01618. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9FFN4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3AE3F384A592A5D4	FASTA	718	79,759
	10 20 30 40 50 60 MEMGRLVFGL CLILIVGTFL EFSGGATAAK GVTYDGRSLI IDGQRKLLFS GSIHYPRSTP 70 80 90 100 110 120 EMWPSLIKKT KEGGIDVIQT YVFWNLHEPK LGQYDFSGRN DLVKFIKEIR SQGLYVCLRI 130 140 150 160 170 180 GPFIEAEWNY GGLPFWLRDV PGMVYRTDNE PFKFHMQKFT AKIVDLMKSE GLYASQGGPI 190 200 210 220 230 240 ILSQIENEYA NVEGAFHEKG ASYIKWAGQM AVGLKTGVPW IMCKSPDAPD PVINTCNGMK 250 260 270 280 290 300 CGETFPGPNS PNKPKMWTED WTSFFQVYGK EPYIRSAEDI AFHAALFVAK NGSYINYYMY 310 320 330 340 350 360 HGGTNFGRTS SSYFITGYYD QAPLDEYGLL RQPKYGHLKE LHAAIKSSAN PLLQGKQTIL 370 380 390 400 410 420 SLGPMQQAYV FEDANNGCVA FLVNNDAKAS QIQFRNNAYS LSPKSIGILQ NCKNLIYETA 430 440 450 460 470 480 KVNVKMNTRV TTPVQVFNVP DNWNLFRETI PAFPGTSLKT NALLEHTNLT KDKTDYLWYT 490 500 510 520 530 540 SSFKLDSPCT NPSIYTESSG HVVHVFVNNA LAGSGHGSRD IRVVKLQAPV SLINGQNNIS 550 560 570 580 590 600 ILSGMVGLPD SGAYMERRSY GLTKVQISCG GTKPIDLSRS QWGYSVGLLG EKVRLYQWKN 610 620 630 640 650 660 LNRVKWSMNK AGLIKNRPLA WYKTTFDGPN GDGPVGLHMS SMGKGEIWVN GESIGRYWVS 670 680 690 700 710 FLTPAGQPSQ SIYHIPRAFL KPSGNLLVVF EEEGGDPLGI SLNTISVVGS SQAQSQFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana." Gy I., Kreis M., Lecharny A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana." Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I. Plant Cell Physiol. 47:55-63(2006) [PubMed: 16267099] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35." Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C. Phytochemistry 68:1510-1520(2007) [PubMed: 17466346] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ270302 mRNA. Translation: CAB64742.1. Frameshift. AB005234 Genomic DNA. Translation: BAB10473.1. AK229786 mRNA. Translation: BAF01618.1.
IPI	IPI00521733.
RefSeq	NP_568978.2.
UniGene	At.21611
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q9FFN4.
Genome annotation databases
GeneID	836500.
GenomeReviews	Gene locus AT5G63800 in contig BA000015_GR.
KEGG	ath:AT5G63800.
NMPDR	fig|3702.1.peg.28431.
Organism-specific databases
GeneFarm	495. 90.
TAIR	At5g63800.
Phylogenomic databases
OMA	HIPRAFL.
Enzyme and pathway databases
BRENDA	3.2.1.23. 302.
Gene expression databases
Genevestigator	Q9FFN4.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL6_ARATH

Accession

Primary (citable) accession number: Q9FFN4
Secondary accession number(s): Q0WMN2, Q9SCV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)