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Q9FFN4 (BGAL6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 6

Short name=Lactase 6
EC=3.2.1.23
Gene names
Name:BGAL6
Ordered Locus Names:At5g63800
ORF Names:MBK5.28
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Expressed at low levels in siliques. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Sequence caution

The sequence CAB64742.1 differs from that shown. Reason: Frameshift at positions 453 and 457.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 718690Beta-galactosidase 6
PRO_5000065881

Sites

Active site1881Proton donor Potential
Active site2591Nucleophile Potential

Amino acid modifications

Glycosylation2911N-linked (GlcNAc...) Potential
Glycosylation4681N-linked (GlcNAc...) Potential
Glycosylation5381N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict701T → A in BAF01618. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9FFN4 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 3AE3F384A592A5D4

FASTA71879,759
        10         20         30         40         50         60 
MEMGRLVFGL CLILIVGTFL EFSGGATAAK GVTYDGRSLI IDGQRKLLFS GSIHYPRSTP 

        70         80         90        100        110        120 
EMWPSLIKKT KEGGIDVIQT YVFWNLHEPK LGQYDFSGRN DLVKFIKEIR SQGLYVCLRI 

       130        140        150        160        170        180 
GPFIEAEWNY GGLPFWLRDV PGMVYRTDNE PFKFHMQKFT AKIVDLMKSE GLYASQGGPI 

       190        200        210        220        230        240 
ILSQIENEYA NVEGAFHEKG ASYIKWAGQM AVGLKTGVPW IMCKSPDAPD PVINTCNGMK 

       250        260        270        280        290        300 
CGETFPGPNS PNKPKMWTED WTSFFQVYGK EPYIRSAEDI AFHAALFVAK NGSYINYYMY 

       310        320        330        340        350        360 
HGGTNFGRTS SSYFITGYYD QAPLDEYGLL RQPKYGHLKE LHAAIKSSAN PLLQGKQTIL 

       370        380        390        400        410        420 
SLGPMQQAYV FEDANNGCVA FLVNNDAKAS QIQFRNNAYS LSPKSIGILQ NCKNLIYETA 

       430        440        450        460        470        480 
KVNVKMNTRV TTPVQVFNVP DNWNLFRETI PAFPGTSLKT NALLEHTNLT KDKTDYLWYT 

       490        500        510        520        530        540 
SSFKLDSPCT NPSIYTESSG HVVHVFVNNA LAGSGHGSRD IRVVKLQAPV SLINGQNNIS 

       550        560        570        580        590        600 
ILSGMVGLPD SGAYMERRSY GLTKVQISCG GTKPIDLSRS QWGYSVGLLG EKVRLYQWKN 

       610        620        630        640        650        660 
LNRVKWSMNK AGLIKNRPLA WYKTTFDGPN GDGPVGLHMS SMGKGEIWVN GESIGRYWVS 

       670        680        690        700        710 
FLTPAGQPSQ SIYHIPRAFL KPSGNLLVVF EEEGGDPLGI SLNTISVVGS SQAQSQFS 

« Hide

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ270302 mRNA. Translation: CAB64742.1. Frameshift.
AB005234 Genomic DNA. Translation: BAB10473.1.
CP002688 Genomic DNA. Translation: AED97799.1.
AK229786 mRNA. Translation: BAF01618.1.
RefSeqNP_568978.2. NM_125775.3.
UniGeneAt.21611.

3D structure databases

ProteinModelPortalQ9FFN4.
SMRQ9FFN4. Positions 30-400, 489-517, 615-694.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G63800.1-P.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9FFN4.
PRIDEQ9FFN4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G63800.1; AT5G63800.1; AT5G63800.
GeneID836500.
KEGGath:AT5G63800.

Organism-specific databases

GeneFarm495. 90.
TAIRAT5G63800.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9FFN4.
OMANTCNGMR.
PhylomeDBQ9FFN4.
ProtClustDBCLSN2918437.

Enzyme and pathway databases

BioCycARA:AT5G63800-MONOMER.

Gene expression databases

ArrayExpressQ9FFN4.
GenevestigatorQ9FFN4.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL6_ARATH
AccessionPrimary (citable) accession number: Q9FFN4
Secondary accession number(s): Q0WMN2, Q9SCV6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 1, 2001
Last modified: March 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names