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Protein

Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH1

Gene

SUVH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Zinc 1By similarity
Metal bindingi434 – 4341Zinc 2By similarity
Metal bindingi436 – 4361Zinc 1By similarity
Metal bindingi440 – 4401Zinc 1By similarity
Metal bindingi440 – 4401Zinc 3By similarity
Metal bindingi447 – 4471Zinc 1By similarity
Metal bindingi449 – 4491Zinc 2By similarity
Metal bindingi475 – 4751Zinc 2By similarity
Metal bindingi475 – 4751Zinc 3By similarity
Metal bindingi479 – 4791Zinc 2By similarity
Metal bindingi481 – 4811Zinc 3By similarity
Metal bindingi484 – 4841Zinc 3By similarity
Binding sitei541 – 5411S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei543 – 5431S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei593 – 5931S-adenosyl-L-methioninePROSITE-ProRule annotation
Metal bindingi599 – 5991Zinc 4By similarity
Metal bindingi658 – 6581Zinc 4By similarity
Metal bindingi660 – 6601Zinc 4By similarity
Metal bindingi665 – 6651Zinc 4By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of gene expression, epigenetic Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G04940-MONOMER.
ARA:GQT-1962-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH1 (EC:2.1.1.43)
Alternative name(s):
Histone H3-K9 methyltransferase 1
Short name:
H3-K9-HMTase 1
Protein SET DOMAIN GROUP 32
Suppressor of variegation 3-9 homolog protein 1
Short name:
Su(var)3-9 homolog protein 1
Gene namesi
Name:SUVH1
Synonyms:SDG32, SET32
Ordered Locus Names:At5g04940
ORF Names:MUG13.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G04940.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH1PRO_0000186072Add
BLAST

Proteomic databases

PaxDbiQ9FF80.
PRIDEiQ9FF80.

Expressioni

Tissue specificityi

Expressed in leaves stems and flowers.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G04940.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FF80.
SMRiQ9FF80. Positions 149-666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 357147YDGPROSITE-ProRule annotationAdd
BLAST
Domaini432 – 49261Pre-SETPROSITE-ProRule annotationAdd
BLAST
Domaini495 – 639145SETPROSITE-ProRule annotationAdd
BLAST
Domaini654 – 67017Post-SETPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni505 – 5073S-adenosyl-L-methionine bindingBy similarity
Regioni596 – 5972S-adenosyl-L-methionine bindingBy similarity

Domaini

Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity.
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.By similarity

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.PROSITE-ProRule annotation
Contains 1 post-SET domain.PROSITE-ProRule annotation
Contains 1 pre-SET domain.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation
Contains 1 YDG domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2940.
HOGENOMiHOG000238382.
InParanoidiQ9FF80.
KOiK11420.
OMAiHECGPTC.
PhylomeDBiQ9FF80.

Family and domain databases

Gene3Di2.30.280.10. 1 hit.
InterProiIPR025794. Hist-Lys_N-MeTrfase_plant.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR015947. PUA-like_domain.
IPR001214. SET_dom.
IPR003105. SRA_YDG.
[Graphical view]
PfamiPF05033. Pre-SET. 1 hit.
PF02182. SAD_SRA. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
SM00466. SRA. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 1 hit.
PROSITEiPS50868. POST_SET. 1 hit.
PS50867. PRE_SET. 1 hit.
PS51575. SAM_MT43_SUVAR39_2. 1 hit.
PS50280. SET. 1 hit.
PS51015. YDG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FF80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERNGGHYTD KTRVLDIKPL RTLRPVFPSG NQAPPFVCAP PFGPFPPGFS
60 70 80 90 100
SFYPFSSSQA NQHTPDLNQA QYPPQHQQPQ NPPPVYQQQP PQHASEPSLV
110 120 130 140 150
TPLRSFRSPD VSNGNAELEG STVKRRIPKK RPISRPENMN FESGINVADR
160 170 180 190 200
ENGNRELVLS VLMRFDALRR RFAQLEDAKE AVSGIIKRPD LKSGSTCMGR
210 220 230 240 250
GVRTNTKKRP GIVPGVEIGD VFFFRFEMCL VGLHSPSMAG IDYLVVKGET
260 270 280 290 300
EEEPIATSIV SSGYYDNDEG NPDVLIYTGQ GGNADKDKQS SDQKLERGNL
310 320 330 340 350
ALEKSLRRDS AVRVIRGLKE ASHNAKIYIY DGLYEIKESW VEKGKSGHNT
360 370 380 390 400
FKYKLVRAPG QPPAFASWTA IQKWKTGVPS RQGLILPDMT SGVESIPVSL
410 420 430 440 450
VNEVDTDNGP AYFTYSTTVK YSESFKLMQP SFGCDCANLC KPGNLDCHCI
460 470 480 490 500
RKNGGDFPYT GNGILVSRKP MIYECSPSCP CSTCKNKVTQ MGVKVRLEVF
510 520 530 540 550
KTANRGWGLR SWDAIRAGSF ICIYVGEAKD KSKVQQTMAN DDYTFDTTNV
560 570 580 590 600
YNPFKWNYEP GLADEDACEE MSEESEIPLP LIISAKNVGN VARFMNHSCS
610 620 630 640 650
PNVFWQPVSY ENNSQLFVHV AFFAISHIPP MTELTYDYGV SRPSGTQNGN
660 670
PLYGKRKCFC GSAYCRGSFG
Length:670
Mass (Da):74,471
Last modified:March 1, 2001 - v1
Checksum:iC92CE89FF5C630F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF344444 mRNA. Translation: AAK28966.1.
AB005245 Genomic DNA. Translation: BAB11516.1.
CP002688 Genomic DNA. Translation: AED90806.1.
CP002688 Genomic DNA. Translation: AED90807.1.
RefSeqiNP_196113.1. NM_120576.1.
NP_850767.1. NM_180436.1.
UniGeneiAt.8375.

Genome annotation databases

EnsemblPlantsiAT5G04940.1; AT5G04940.1; AT5G04940.
AT5G04940.2; AT5G04940.2; AT5G04940.
GeneIDi830376.
KEGGiath:AT5G04940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF344444 mRNA. Translation: AAK28966.1.
AB005245 Genomic DNA. Translation: BAB11516.1.
CP002688 Genomic DNA. Translation: AED90806.1.
CP002688 Genomic DNA. Translation: AED90807.1.
RefSeqiNP_196113.1. NM_120576.1.
NP_850767.1. NM_180436.1.
UniGeneiAt.8375.

3D structure databases

ProteinModelPortaliQ9FF80.
SMRiQ9FF80. Positions 149-666.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G04940.1.

Proteomic databases

PaxDbiQ9FF80.
PRIDEiQ9FF80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G04940.1; AT5G04940.1; AT5G04940.
AT5G04940.2; AT5G04940.2; AT5G04940.
GeneIDi830376.
KEGGiath:AT5G04940.

Organism-specific databases

TAIRiAT5G04940.

Phylogenomic databases

eggNOGiCOG2940.
HOGENOMiHOG000238382.
InParanoidiQ9FF80.
KOiK11420.
OMAiHECGPTC.
PhylomeDBiQ9FF80.

Enzyme and pathway databases

BioCyciARA:AT5G04940-MONOMER.
ARA:GQT-1962-MONOMER.

Miscellaneous databases

PROiQ9FF80.

Family and domain databases

Gene3Di2.30.280.10. 1 hit.
InterProiIPR025794. Hist-Lys_N-MeTrfase_plant.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR015947. PUA-like_domain.
IPR001214. SET_dom.
IPR003105. SRA_YDG.
[Graphical view]
PfamiPF05033. Pre-SET. 1 hit.
PF02182. SAD_SRA. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
SM00466. SRA. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 1 hit.
PROSITEiPS50868. POST_SET. 1 hit.
PS50867. PRE_SET. 1 hit.
PS51575. SAM_MT43_SUVAR39_2. 1 hit.
PS50280. SET. 1 hit.
PS51015. YDG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes."
    Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B.
    Nucleic Acids Res. 29:4319-4333(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, NOMENCLATURE, TISSUE SPECIFICITY.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis."
    Naumann K., Fischer A., Hofmann I., Krauss V., Phalke S., Irmler K., Hause G., Aurich A.-C., Dorn R., Jenuwein T., Reuter G.
    EMBO J. 24:1418-1429(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Heterochromatin proteins and the control of heterochromatic gene silencing in Arabidopsis."
    Fischer A., Hofmann I., Naumann K., Reuter G.
    J. Plant Physiol. 163:358-368(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiSUVH1_ARATH
AccessioniPrimary (citable) accession number: Q9FF80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.