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Q9FF77 (PME47_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable pectinesterase/pectinesterase inhibitor 47

Including the following 2 domains:

  1. Pectinesterase inhibitor 47
    Alternative name(s):
    Pectin methylesterase inhibitor 47
  2. Pectinesterase 47
    Short name=PE 47
    EC=3.1.1.11
    Alternative name(s):
    Pectin methylesterase 47
    Short name=AtPME47
Gene names
Name:PME47
Synonyms:ARATH47
Ordered Locus Names:At5g04970
ORF Names:MUG13.17
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length624 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.

Catalytic activity

Pectin + n H2O = n methanol + pectate.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subcellular location

Secreted › cell wall By similarity.

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Sequence similarities

In the N-terminal section; belongs to the PMEI family.

In the C-terminal section; belongs to the pectinesterase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 624605Probable pectinesterase/pectinesterase inhibitor 47
PRO_0000371695

Regions

Region74 – 236163Pectinesterase inhibitor 47
Region307 – 606300Pectinesterase 47
Compositional bias24 – 9471Pro-rich

Sites

Active site4381Proton donor; for pectinesterase activity By similarity
Active site4591Nucleophile; for pectinesterase activity By similarity
Binding site3851Substrate; for pectinesterase activity By similarity
Binding site4151Substrate; for pectinesterase activity By similarity
Binding site5271Substrate; for pectinesterase activity By similarity
Binding site5291Substrate; for pectinesterase activity By similarity
Site4371Transition state stabilizer By similarity

Amino acid modifications

Glycosylation2251N-linked (GlcNAc...) Potential
Glycosylation3301N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation3761N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential
Glycosylation5051N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential
Glycosylation5961N-linked (GlcNAc...) Potential
Glycosylation6011N-linked (GlcNAc...) Potential
Disulfide bond452 ↔ 472 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FF77 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 80D388C903AC5E9A

FASTA62468,148
        10         20         30         40         50         60 
MQTHSSSLVF LALLCLSWAL LVSPTRPPSQ PPSHPPIQPS SQPPTQPPSQ PPTQPPTQPP 

        70         80         90        100        110        120 
SHPPTQPPTP PPSQSPSQPS PLPPNIACKS TPYPKLCRTI LSAVKSSPSD PYHYGKFTMK 

       130        140        150        160        170        180 
QCLKQARRLS KVINRFAQRV EADPGTSTVE EVSAVADCGE LAELSVEYLE TVTEELKAAE 

       190        200        210        220        230        240 
LMTAALVDRV TSLLGGVVTN QQTCLDGLVD AKSGFATAIG TPLGNLTRLY SVSLGLVSHA 

       250        260        270        280        290        300 
LNRNLKRYKG SKGKIFGGGN KPVREPLETL IKVLRKTCDK GKDCRKANRN LGELGETSGG 

       310        320        330        340        350        360 
SILVREAVTV GPYETDNFPT ITEAVAAAPN HTFPEQGYFV IYARAGLYEE YVVISNKKRN 

       370        380        390        400        410        420 
IMLIGDGINK TIISGNHSFI DGWTTYNSST FAVVGDRFVA VDVTFRNTAG PEKHQAVAVR 

       430        440        450        460        470        480 
NNADGSTFYR CSFEGYQDTL YVHSLRQFYR ECDIYGTIDF IFGNAAAIFQ NCNIYARKPM 

       490        500        510        520        530        540 
ANQKNAVTAH GRTDPNQKTG ISIINCTIGA APDLAADPKS TMTFLGRPWK PYSRTVYIQS 

       550        560        570        580        590        600 
YISDVVQPVG WLEWNGTTGL DTISYGEYDN FGPGADTSKR VQWSGYSLLN LVQAMNFTVY 

       610        620 
NFTLGDTWLP QTDIPFYGGL LHTE

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Pectin methylesterases: sequence-structural features and phylogenetic relationships."
Markovic O., Janecek S.
Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB005245 Genomic DNA. Translation: BAB11519.1.
CP002688 Genomic DNA. Translation: AED90810.1.
IPIIPI00544660.
RefSeqNP_196116.1. NM_120579.2.
UniGeneAt.27773.

3D structure databases

HSSPHSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ProteinModelPortalQ9FF77.
SMRQ9FF77. Positions 303-620.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9FF77.

Proteomic databases

PRIDEQ9FF77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G04970.1; AT5G04970.1; AT5G04970.
GeneID830379.
GenomeReviewsGene locus AT5G04970 in contig BA000015_GR.
KEGGath:AT5G04970.
NMPDRfig|3702.1.peg.22596.

Organism-specific databases

GeneFarm439. 8.
TAIRAt5g04970.

Phylogenomic databases

eggNOGCOG4677.
GeneTreeEPGT00070000027901.
HOGENOMHBG747179.
InParanoidQ9FF77.
OMANIACKST.
PhylomeDBQ9FF77.
ProtClustDBPLN03043.

Gene expression databases

GenevestigatorQ9FF77.

Family and domain databases

InterProIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
KOK01051.
PfamPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
SSF101148. Pectinesterase_inhib. 1 hit.
PROSITEPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePME47_ARATH
AccessionPrimary (citable) accession number: Q9FF77
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2001
Last modified: December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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