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Protein

Ubiquitin-conjugating enzyme E2 22

Gene

UBC22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-like protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central
  6. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. protein polyubiquitination Source: GO_Central
  2. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G05080-MONOMER.
ARA:GQT-1854-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 22 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 22
Gene namesi
Name:UBC22
Ordered Locus Names:At5g05080
ORF Names:MUG13.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G05080.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Ubiquitin-conjugating enzyme E2 22PRO_0000345188Add
BLAST

Post-translational modificationi

Self-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9FF66.
PRIDEiQ9FF66.

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Inductioni

By the herbicide isoxaben.1 Publication

Gene expression databases

GenevestigatoriQ9FF66.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05080.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FF66.
SMRiQ9FF66. Positions 5-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili230 – 25122Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ9FF66.
KOiK10583.
OMAiLGIQDQN.
PhylomeDBiQ9FF66.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FF66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNENLPPN VIKQLAKELK SLDESPPDGI KVVVNDEDFS QICADIEGPV
60 70 80 90 100
GTPYENGLFR MKLALSHDFP HSPPKGYFMT KIFHPNVASN GEICVNTLKK
110 120 130 140 150
DWNPSLGLRH VLSVVRCLLI EPFPESALNE QAGKMLLENY DEYARHARLY
160 170 180 190 200
TGIHAKPKPK FKTGAISEST TALNVGQTNN ETPGAATAIP SSMTDIKRVT
210 220 230 240 250
TSAQDQQHVA NVVVAASASV VTTTQKREAG LAKVQADKKK VDARKKSLKR

L
Length:251
Mass (Da):27,399
Last modified:March 1, 2001 - v1
Checksum:i3A335129A16D616E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027036 mRNA. Translation: AAY44862.1.
AB005245 Genomic DNA. Translation: BAB11530.1.
CP002688 Genomic DNA. Translation: AED90825.1.
CP002688 Genomic DNA. Translation: AED90826.1.
AY116948 mRNA. Translation: AAM51582.1.
AF428320 mRNA. Translation: AAL16250.1.
AY088106 mRNA. Translation: AAM65652.1.
RefSeqiNP_001078530.1. NM_001085061.1.
NP_568148.1. NM_120590.2.
UniGeneiAt.23123.

Genome annotation databases

EnsemblPlantsiAT5G05080.1; AT5G05080.1; AT5G05080.
AT5G05080.2; AT5G05080.2; AT5G05080.
GeneIDi830390.
KEGGiath:AT5G05080.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027036 mRNA. Translation: AAY44862.1.
AB005245 Genomic DNA. Translation: BAB11530.1.
CP002688 Genomic DNA. Translation: AED90825.1.
CP002688 Genomic DNA. Translation: AED90826.1.
AY116948 mRNA. Translation: AAM51582.1.
AF428320 mRNA. Translation: AAL16250.1.
AY088106 mRNA. Translation: AAM65652.1.
RefSeqiNP_001078530.1. NM_001085061.1.
NP_568148.1. NM_120590.2.
UniGeneiAt.23123.

3D structure databases

ProteinModelPortaliQ9FF66.
SMRiQ9FF66. Positions 5-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G05080.1-P.

Proteomic databases

PaxDbiQ9FF66.
PRIDEiQ9FF66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G05080.1; AT5G05080.1; AT5G05080.
AT5G05080.2; AT5G05080.2; AT5G05080.
GeneIDi830390.
KEGGiath:AT5G05080.

Organism-specific databases

TAIRiAT5G05080.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ9FF66.
KOiK10583.
OMAiLGIQDQN.
PhylomeDBiQ9FF66.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT5G05080-MONOMER.
ARA:GQT-1854-MONOMER.

Miscellaneous databases

PROiQ9FF66.

Gene expression databases

GenevestigatoriQ9FF66.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, UBIQUITINATION, GENE FAMILY, NOMENCLATURE.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiUBC22_ARATH
AccessioniPrimary (citable) accession number: Q9FF66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.