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Q9FF66

- UBC22_ARATH

UniProt

Q9FF66 - UBC22_ARATH

Protein

Ubiquitin-conjugating enzyme E2 22

Gene

UBC22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei94 – 941Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G05080-MONOMER.
    ARA:GQT-1854-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 22 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 22
    Gene namesi
    Name:UBC22
    Ordered Locus Names:At5g05080
    ORF Names:MUG13.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G05080.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Ubiquitin-conjugating enzyme E2 22PRO_0000345188Add
    BLAST

    Post-translational modificationi

    Self-ubiquitinated.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ9FF66.
    PRIDEiQ9FF66.

    Expressioni

    Tissue specificityi

    Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

    Inductioni

    By the herbicide isoxaben.1 Publication

    Gene expression databases

    GenevestigatoriQ9FF66.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G05080.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FF66.
    SMRiQ9FF66. Positions 5-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili230 – 25122Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiQ9FF66.
    KOiK10583.
    OMAiQICADIE.
    PhylomeDBiQ9FF66.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FF66-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASNENLPPN VIKQLAKELK SLDESPPDGI KVVVNDEDFS QICADIEGPV    50
    GTPYENGLFR MKLALSHDFP HSPPKGYFMT KIFHPNVASN GEICVNTLKK 100
    DWNPSLGLRH VLSVVRCLLI EPFPESALNE QAGKMLLENY DEYARHARLY 150
    TGIHAKPKPK FKTGAISEST TALNVGQTNN ETPGAATAIP SSMTDIKRVT 200
    TSAQDQQHVA NVVVAASASV VTTTQKREAG LAKVQADKKK VDARKKSLKR 250
    L 251
    Length:251
    Mass (Da):27,399
    Last modified:March 1, 2001 - v1
    Checksum:i3A335129A16D616E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027036 mRNA. Translation: AAY44862.1.
    AB005245 Genomic DNA. Translation: BAB11530.1.
    CP002688 Genomic DNA. Translation: AED90825.1.
    CP002688 Genomic DNA. Translation: AED90826.1.
    AY116948 mRNA. Translation: AAM51582.1.
    AF428320 mRNA. Translation: AAL16250.1.
    AY088106 mRNA. Translation: AAM65652.1.
    RefSeqiNP_001078530.1. NM_001085061.1.
    NP_568148.1. NM_120590.2.
    UniGeneiAt.23123.

    Genome annotation databases

    EnsemblPlantsiAT5G05080.1; AT5G05080.1; AT5G05080.
    AT5G05080.2; AT5G05080.2; AT5G05080.
    GeneIDi830390.
    KEGGiath:AT5G05080.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027036 mRNA. Translation: AAY44862.1 .
    AB005245 Genomic DNA. Translation: BAB11530.1 .
    CP002688 Genomic DNA. Translation: AED90825.1 .
    CP002688 Genomic DNA. Translation: AED90826.1 .
    AY116948 mRNA. Translation: AAM51582.1 .
    AF428320 mRNA. Translation: AAL16250.1 .
    AY088106 mRNA. Translation: AAM65652.1 .
    RefSeqi NP_001078530.1. NM_001085061.1.
    NP_568148.1. NM_120590.2.
    UniGenei At.23123.

    3D structure databases

    ProteinModelPortali Q9FF66.
    SMRi Q9FF66. Positions 5-155.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G05080.1-P.

    Proteomic databases

    PaxDbi Q9FF66.
    PRIDEi Q9FF66.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G05080.1 ; AT5G05080.1 ; AT5G05080 .
    AT5G05080.2 ; AT5G05080.2 ; AT5G05080 .
    GeneIDi 830390.
    KEGGi ath:AT5G05080.

    Organism-specific databases

    TAIRi AT5G05080.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi Q9FF66.
    KOi K10583.
    OMAi QICADIE.
    PhylomeDBi Q9FF66.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G05080-MONOMER.
    ARA:GQT-1854-MONOMER.

    Miscellaneous databases

    PROi Q9FF66.

    Gene expression databases

    Genevestigatori Q9FF66.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, UBIQUITINATION, GENE FAMILY, NOMENCLATURE.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiUBC22_ARATH
    AccessioniPrimary (citable) accession number: Q9FF66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3