Q9FF66 (UBC22_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 22
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 22

Gene names

Name:	UBC22
Ordered Locus Names:	At5g05080
ORF Names:	MUG13.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Expressed in seeds, pistils, siliques, hypocotyls and leaves. Ref.1
Induction	By the herbicide isoxaben. Ref.1
Post-translational modification	Self-ubiquitinated.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Ubiquitin-conjugating enzyme E2 22		PRO_0000345188
Regions
	Coiled coil	230 – 251	22	Potential
Sites
	Active site	94	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FF66 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3A335129A16D616E	FASTA	251	27,399
	10 20 30 40 50 60 MASNENLPPN VIKQLAKELK SLDESPPDGI KVVVNDEDFS QICADIEGPV GTPYENGLFR 70 80 90 100 110 120 MKLALSHDFP HSPPKGYFMT KIFHPNVASN GEICVNTLKK DWNPSLGLRH VLSVVRCLLI 130 140 150 160 170 180 EPFPESALNE QAGKMLLENY DEYARHARLY TGIHAKPKPK FKTGAISEST TALNVGQTNN 190 200 210 220 230 240 ETPGAATAIP SSMTDIKRVT TSAQDQQHVA NVVVAASASV VTTTQKREAG LAKVQADKKK 250 VDARKKSLKR L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, UBIQUITINATION, GENE FAMILY, NOMENCLATURE.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ027036 mRNA. Translation: AAY44862.1. AB005245 Genomic DNA. Translation: BAB11530.1. CP002688 Genomic DNA. Translation: AED90825.1. CP002688 Genomic DNA. Translation: AED90826.1. AY116948 mRNA. Translation: AAM51582.1. AF428320 mRNA. Translation: AAL16250.1. AY088106 mRNA. Translation: AAM65652.1.
IPI	IPI00519454.
RefSeq	NP_001078530.1. NM_001085061.1. NP_568148.1. NM_120590.2.
UniGene	At.23123.
3D structure databases
HSSP	HSSP built from PDB template 1ZDN based on UniProtKB Q16763.
ProteinModelPortal	Q9FF66.
SMR	Q9FF66. Positions 5-155.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G05080.1-P.
Proteomic databases
PaxDb	Q9FF66.
PRIDE	Q9FF66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G05080.1; AT5G05080.1; AT5G05080. AT5G05080.2; AT5G05080.2; AT5G05080.
GeneID	830390.
KEGG	ath:AT5G05080.
Organism-specific databases
TAIR	At5g05080.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
InParanoid	Q9FF66.
KO	K10583.
OMA	QICADIE.
PhylomeDB	Q9FF66.
ProtClustDB	CLSN2689475.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q9FF66.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC22_ARATH

Accession

Primary (citable) accession number: Q9FF66

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program