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Q9FF66

- UBC22_ARATH

UniProt

Q9FF66 - UBC22_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 22

Gene
UBC22, At5g05080, MUG13.6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G05080-MONOMER.
ARA:GQT-1854-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 22 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 22
Gene namesi
Name:UBC22
Ordered Locus Names:At5g05080
ORF Names:MUG13.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G05080.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Ubiquitin-conjugating enzyme E2 22PRO_0000345188Add
BLAST

Post-translational modificationi

Self-ubiquitinated.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9FF66.
PRIDEiQ9FF66.

Expressioni

Tissue specificityi

Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

Inductioni

By the herbicide isoxaben.1 Publication

Gene expression databases

GenevestigatoriQ9FF66.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G05080.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FF66.
SMRiQ9FF66. Positions 5-155.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili230 – 25122 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiQ9FF66.
KOiK10583.
OMAiQICADIE.
PhylomeDBiQ9FF66.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FF66-1 [UniParc]FASTAAdd to Basket

« Hide

MASNENLPPN VIKQLAKELK SLDESPPDGI KVVVNDEDFS QICADIEGPV    50
GTPYENGLFR MKLALSHDFP HSPPKGYFMT KIFHPNVASN GEICVNTLKK 100
DWNPSLGLRH VLSVVRCLLI EPFPESALNE QAGKMLLENY DEYARHARLY 150
TGIHAKPKPK FKTGAISEST TALNVGQTNN ETPGAATAIP SSMTDIKRVT 200
TSAQDQQHVA NVVVAASASV VTTTQKREAG LAKVQADKKK VDARKKSLKR 250
L 251
Length:251
Mass (Da):27,399
Last modified:March 1, 2001 - v1
Checksum:i3A335129A16D616E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027036 mRNA. Translation: AAY44862.1.
AB005245 Genomic DNA. Translation: BAB11530.1.
CP002688 Genomic DNA. Translation: AED90825.1.
CP002688 Genomic DNA. Translation: AED90826.1.
AY116948 mRNA. Translation: AAM51582.1.
AF428320 mRNA. Translation: AAL16250.1.
AY088106 mRNA. Translation: AAM65652.1.
RefSeqiNP_001078530.1. NM_001085061.1.
NP_568148.1. NM_120590.2.
UniGeneiAt.23123.

Genome annotation databases

EnsemblPlantsiAT5G05080.1; AT5G05080.1; AT5G05080.
AT5G05080.2; AT5G05080.2; AT5G05080.
GeneIDi830390.
KEGGiath:AT5G05080.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ027036 mRNA. Translation: AAY44862.1 .
AB005245 Genomic DNA. Translation: BAB11530.1 .
CP002688 Genomic DNA. Translation: AED90825.1 .
CP002688 Genomic DNA. Translation: AED90826.1 .
AY116948 mRNA. Translation: AAM51582.1 .
AF428320 mRNA. Translation: AAL16250.1 .
AY088106 mRNA. Translation: AAM65652.1 .
RefSeqi NP_001078530.1. NM_001085061.1.
NP_568148.1. NM_120590.2.
UniGenei At.23123.

3D structure databases

ProteinModelPortali Q9FF66.
SMRi Q9FF66. Positions 5-155.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G05080.1-P.

Proteomic databases

PaxDbi Q9FF66.
PRIDEi Q9FF66.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G05080.1 ; AT5G05080.1 ; AT5G05080 .
AT5G05080.2 ; AT5G05080.2 ; AT5G05080 .
GeneIDi 830390.
KEGGi ath:AT5G05080.

Organism-specific databases

TAIRi AT5G05080.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
InParanoidi Q9FF66.
KOi K10583.
OMAi QICADIE.
PhylomeDBi Q9FF66.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G05080-MONOMER.
ARA:GQT-1854-MONOMER.

Miscellaneous databases

PROi Q9FF66.

Gene expression databases

Genevestigatori Q9FF66.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, UBIQUITINATION, GENE FAMILY, NOMENCLATURE.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiUBC22_ARATH
AccessioniPrimary (citable) accession number: Q9FF66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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