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Q9FF55

- PDI14_ARATH

UniProt

Q9FF55 - PDI14_ARATH

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Protein

Protein disulfide isomerase-like 1-4

Gene

PDIL1-4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei132 – 1321NucleophileBy similarity
Sitei133 – 1331Contributes to redox potential valueBy similarity
Sitei134 – 1341Contributes to redox potential valueBy similarity
Active sitei135 – 1351NucleophileBy similarity
Sitei194 – 1941Lowers pKa of C-terminal Cys of first active siteBy similarity
Active sitei471 – 4711NucleophileBy similarity
Sitei472 – 4721Contributes to redox potential valueBy similarity
Sitei473 – 4731Contributes to redox potential valueBy similarity
Active sitei474 – 4741NucleophileBy similarity
Sitei536 – 5361Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. protein folding Source: GOC
  3. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT5G60640-MONOMER.
ARA:GQT-84-MONOMER.
ARA:GQT-85-MONOMER.
ReactomeiREACT_187630. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide isomerase-like 1-4 (EC:5.3.4.1)
Short name:
AtPDIL1-4
Alternative name(s):
Protein disulfide isomerase 2
Short name:
AtPDI2
Protein disulfide isomerase-like 2-2
Short name:
AtPDIL2-2
Gene namesi
Name:PDIL1-4
Synonyms:PDI2, PDIL2-2
Ordered Locus Names:At5g60640
ORF Names:MUP24.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G60640.

Subcellular locationi

Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation. Golgi apparatus 1 Publication. Vacuole 1 Publication. Nucleus 1 Publication. Secretedcell wall 1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-KW
  2. chloroplast Source: TAIR
  3. endoplasmic reticulum Source: TAIR
  4. extracellular region Source: UniProtKB-KW
  5. mitochondrion Source: TAIR
  6. nucleus Source: UniProtKB-KW
  7. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, probably due to functional redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 597572Protein disulfide isomerase-like 1-4PRO_0000400019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi132 ↔ 135Redox-activePROSITE-ProRule annotation
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi471 ↔ 474Redox-activePROSITE-ProRule annotation
Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FF55.
PRIDEiQ9FF55.

Expressioni

Tissue specificityi

Expressed in germinating seedling, including the cotyledons and hypocotyl, in vascular tissues, in pollen grains, root tips, leaf trichomes, developing seeds and siliques.2 Publications

Gene expression databases

GenevestigatoriQ9FF55.

Interactioni

Subunit structurei

Interacts with MEE8 and MED37A.1 Publication

Protein-protein interaction databases

BioGridi21429. 10 interactions.
STRINGi3702.AT5G60640.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FF55.
SMRiQ9FF55. Positions 41-550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 208124Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini429 – 550122Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi594 – 5974Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiCOG0526.
HOGENOMiHOG000162459.
InParanoidiQ9FF55.
KOiK09580.
OMAiKHATIPF.
PhylomeDBiQ9FF55.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FF55-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFRVLLLFS LTALLIFSAV SPSFAASSSD DVDDEDLSFL EDLKEDDVPG
60 70 80 90 100
ADSLSSSTGF DEFEGGEEED PDMYNDDDDE EGDFSDLGNP DSDPLPTPEI
110 120 130 140 150
DEKDVVVIKE RNFTDVIENN QYVLVEFYAP WCGHCQSLAP EYAAAATELK
160 170 180 190 200
EDGVVLAKID ATEENELAQE YRVQGFPTLL FFVDGEHKPY TGGRTKETIV
210 220 230 240 250
TWVKKKIGPG VYNLTTLDDA EKVLTSGNKV VLGYLNSLVG VEHDQLNAAS
260 270 280 290 300
KAEDDVNFYQ TVNPDVAKMF HLDPESKRPA LVLVKKEEEK ISHFDGEFVK
310 320 330 340 350
SALVSFVSAN KLALVSVFTR ETAPEIFESA IKKQLLLFVT KNESEKVLTE
360 370 380 390 400
FQEAAKSFKG KLIFVSVDLD NEDYGKPVAE YFGVSGNGPK LIGYTGNEDP
410 420 430 440 450
KKYFFDGEIQ SDKIKIFGED FLNDKLKPFY KSDPIPEKND EDVKIVVGDN
460 470 480 490 500
FDEIVLDDSK DVLLEVYAPW CGHCQALEPM YNKLAKHLRS IDSLVITKMD
510 520 530 540 550
GTTNEHPKAK AEGFPTILFF PAGNKTSEPI TVDTDRTVVA FYKFLRKHAT
560 570 580 590
IPFKLEKPAS TESPKTAEST PKVETTETKE SPDSTTKSSQ SDSKDEL
Length:597
Mass (Da):66,357
Last modified:March 1, 2001 - v1
Checksum:i5A8FC7E72AA64B2B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti400 – 4001P → H in AAM65262. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005246 Genomic DNA. Translation: BAB09837.1.
CP002688 Genomic DNA. Translation: AED97360.1.
BT001994 mRNA. Translation: AAN72005.1.
BT008359 mRNA. Translation: AAP37718.1.
AY087725 mRNA. Translation: AAM65262.1.
RefSeqiNP_851234.1. NM_180903.3. [Q9FF55-1]
UniGeneiAt.22422.

Genome annotation databases

EnsemblPlantsiAT5G60640.1; AT5G60640.1; AT5G60640. [Q9FF55-1]
GeneIDi836185.
KEGGiath:AT5G60640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005246 Genomic DNA. Translation: BAB09837.1 .
CP002688 Genomic DNA. Translation: AED97360.1 .
BT001994 mRNA. Translation: AAN72005.1 .
BT008359 mRNA. Translation: AAP37718.1 .
AY087725 mRNA. Translation: AAM65262.1 .
RefSeqi NP_851234.1. NM_180903.3. [Q9FF55-1 ]
UniGenei At.22422.

3D structure databases

ProteinModelPortali Q9FF55.
SMRi Q9FF55. Positions 41-550.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21429. 10 interactions.
STRINGi 3702.AT5G60640.1-P.

Proteomic databases

PaxDbi Q9FF55.
PRIDEi Q9FF55.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G60640.1 ; AT5G60640.1 ; AT5G60640 . [Q9FF55-1 ]
GeneIDi 836185.
KEGGi ath:AT5G60640.

Organism-specific databases

TAIRi AT5G60640.

Phylogenomic databases

eggNOGi COG0526.
HOGENOMi HOG000162459.
InParanoidi Q9FF55.
KOi K09580.
OMAi KHATIPF.
PhylomeDBi Q9FF55.

Enzyme and pathway databases

BioCyci ARA:AT5G60640-MONOMER.
ARA:GQT-84-MONOMER.
ARA:GQT-85-MONOMER.
Reactomei REACT_187630. XBP1(S) activates chaperone genes.

Gene expression databases

Genevestigatori Q9FF55.

Family and domain databases

Gene3Di 3.40.30.10. 4 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
    Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
    Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
    Lu D.-P., Christopher D.A.
    Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
    d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
    BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor."
    Cho E.J., Yuen C.Y., Kang B.H., Ondzighi C.A., Staehelin L.A., Christopher D.A.
    Mol. Cells 32:459-475(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MEE8 AND MED37A/BIP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPDI14_ARATH
AccessioniPrimary (citable) accession number: Q9FF55
Secondary accession number(s): Q8LAM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3