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Protein

Protein disulfide isomerase-like 1-4

Gene

PDIL1-4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei132NucleophileBy similarity1
Sitei133Contributes to redox potential valueBy similarity1
Sitei134Contributes to redox potential valueBy similarity1
Active sitei135NucleophileBy similarity1
Sitei194Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei471NucleophileBy similarity1
Sitei472Contributes to redox potential valueBy similarity1
Sitei473Contributes to redox potential valueBy similarity1
Active sitei474NucleophileBy similarity1
Sitei536Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide isomerase-like 1-4 (EC:5.3.4.1)
Short name:
AtPDIL1-4
Alternative name(s):
Protein disulfide isomerase 2
Short name:
AtPDI2
Protein disulfide isomerase-like 2-2
Short name:
AtPDIL2-2
Gene namesi
Name:PDIL1-4
Synonyms:PDI2, PDIL2-2
Ordered Locus Names:At5g60640
ORF Names:MUP24.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G60640.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation1 Publication
  • Golgi apparatus 1 Publication
  • Vacuole 1 Publication
  • Nucleus 1 Publication
  • Secretedcell wall 1 Publication

GO - Cellular componenti

  • cell wall Source: UniProtKB-SubCell
  • chloroplast Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-KW
  • mitochondrion Source: TAIR
  • nucleus Source: UniProtKB-SubCell
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, probably due to functional redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000040001926 – 597Protein disulfide isomerase-like 1-4Add BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi132 ↔ 135Redox-activePROSITE-ProRule annotation
Glycosylationi213N-linked (GlcNAc...)Sequence analysis1
Glycosylationi342N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi471 ↔ 474Redox-activePROSITE-ProRule annotation
Glycosylationi524N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FF55.

PTM databases

iPTMnetiQ9FF55.
SwissPalmiQ9FF55.

Expressioni

Tissue specificityi

Expressed in germinating seedling, including the cotyledons and hypocotyl, in vascular tissues, in pollen grains, root tips, leaf trichomes, developing seeds and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ9FF55. baseline and differential.
GenevisibleiQ9FF55. AT.

Interactioni

Subunit structurei

Interacts with MEE8 and MED37A.1 Publication

Protein-protein interaction databases

BioGridi21429. 11 interactors.
STRINGi3702.AT5G60640.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FF55.
SMRiQ9FF55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 208Thioredoxin 1PROSITE-ProRule annotationAdd BLAST124
Domaini429 – 550Thioredoxin 2PROSITE-ProRule annotationAdd BLAST122

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi594 – 597Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
InParanoidiQ9FF55.
KOiK09580.
OMAiIPEDNEG.
OrthoDBiEOG093607A9.
PhylomeDBiQ9FF55.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9FF55-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFRVLLLFS LTALLIFSAV SPSFAASSSD DVDDEDLSFL EDLKEDDVPG
60 70 80 90 100
ADSLSSSTGF DEFEGGEEED PDMYNDDDDE EGDFSDLGNP DSDPLPTPEI
110 120 130 140 150
DEKDVVVIKE RNFTDVIENN QYVLVEFYAP WCGHCQSLAP EYAAAATELK
160 170 180 190 200
EDGVVLAKID ATEENELAQE YRVQGFPTLL FFVDGEHKPY TGGRTKETIV
210 220 230 240 250
TWVKKKIGPG VYNLTTLDDA EKVLTSGNKV VLGYLNSLVG VEHDQLNAAS
260 270 280 290 300
KAEDDVNFYQ TVNPDVAKMF HLDPESKRPA LVLVKKEEEK ISHFDGEFVK
310 320 330 340 350
SALVSFVSAN KLALVSVFTR ETAPEIFESA IKKQLLLFVT KNESEKVLTE
360 370 380 390 400
FQEAAKSFKG KLIFVSVDLD NEDYGKPVAE YFGVSGNGPK LIGYTGNEDP
410 420 430 440 450
KKYFFDGEIQ SDKIKIFGED FLNDKLKPFY KSDPIPEKND EDVKIVVGDN
460 470 480 490 500
FDEIVLDDSK DVLLEVYAPW CGHCQALEPM YNKLAKHLRS IDSLVITKMD
510 520 530 540 550
GTTNEHPKAK AEGFPTILFF PAGNKTSEPI TVDTDRTVVA FYKFLRKHAT
560 570 580 590
IPFKLEKPAS TESPKTAEST PKVETTETKE SPDSTTKSSQ SDSKDEL
Length:597
Mass (Da):66,357
Last modified:March 1, 2001 - v1
Checksum:i5A8FC7E72AA64B2B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti400P → H in AAM65262 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005246 Genomic DNA. Translation: BAB09837.1.
CP002688 Genomic DNA. Translation: AED97360.1.
BT001994 mRNA. Translation: AAN72005.1.
BT008359 mRNA. Translation: AAP37718.1.
AY087725 mRNA. Translation: AAM65262.1.
RefSeqiNP_851234.1. NM_180903.4. [Q9FF55-1]
UniGeneiAt.22422.

Genome annotation databases

EnsemblPlantsiAT5G60640.1; AT5G60640.1; AT5G60640. [Q9FF55-1]
GeneIDi836185.
GrameneiAT5G60640.1; AT5G60640.1; AT5G60640.
KEGGiath:AT5G60640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005246 Genomic DNA. Translation: BAB09837.1.
CP002688 Genomic DNA. Translation: AED97360.1.
BT001994 mRNA. Translation: AAN72005.1.
BT008359 mRNA. Translation: AAP37718.1.
AY087725 mRNA. Translation: AAM65262.1.
RefSeqiNP_851234.1. NM_180903.4. [Q9FF55-1]
UniGeneiAt.22422.

3D structure databases

ProteinModelPortaliQ9FF55.
SMRiQ9FF55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21429. 11 interactors.
STRINGi3702.AT5G60640.1.

PTM databases

iPTMnetiQ9FF55.
SwissPalmiQ9FF55.

Proteomic databases

PaxDbiQ9FF55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G60640.1; AT5G60640.1; AT5G60640. [Q9FF55-1]
GeneIDi836185.
GrameneiAT5G60640.1; AT5G60640.1; AT5G60640.
KEGGiath:AT5G60640.

Organism-specific databases

TAIRiAT5G60640.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
InParanoidiQ9FF55.
KOiK09580.
OMAiIPEDNEG.
OrthoDBiEOG093607A9.
PhylomeDBiQ9FF55.

Miscellaneous databases

PROiQ9FF55.

Gene expression databases

ExpressionAtlasiQ9FF55. baseline and differential.
GenevisibleiQ9FF55. AT.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDI14_ARATH
AccessioniPrimary (citable) accession number: Q9FF55
Secondary accession number(s): Q8LAM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.