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Q9FF55

- PDI14_ARATH

UniProt

Q9FF55 - PDI14_ARATH

Protein

Protein disulfide isomerase-like 1-4

Gene

PDIL1-4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.1 Publication

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei132 – 1321NucleophileBy similarity
    Sitei133 – 1331Contributes to redox potential valueBy similarity
    Sitei134 – 1341Contributes to redox potential valueBy similarity
    Active sitei135 – 1351NucleophileBy similarity
    Sitei194 – 1941Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei471 – 4711NucleophileBy similarity
    Sitei472 – 4721Contributes to redox potential valueBy similarity
    Sitei473 – 4731Contributes to redox potential valueBy similarity
    Active sitei474 – 4741NucleophileBy similarity
    Sitei536 – 5361Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: TAIR

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. protein folding Source: GOC
    3. response to oxidative stress Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciARA:AT5G60640-MONOMER.
    ARA:GQT-84-MONOMER.
    ARA:GQT-85-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide isomerase-like 1-4 (EC:5.3.4.1)
    Short name:
    AtPDIL1-4
    Alternative name(s):
    Protein disulfide isomerase 2
    Short name:
    AtPDI2
    Protein disulfide isomerase-like 2-2
    Short name:
    AtPDIL2-2
    Gene namesi
    Name:PDIL1-4
    Synonyms:PDI2, PDIL2-2
    Ordered Locus Names:At5g60640
    ORF Names:MUP24.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G60640.

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation. Golgi apparatus 1 Publication. Vacuole 1 Publication. Nucleus 1 Publication. Secretedcell wall 1 Publication

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. chloroplast Source: TAIR
    3. endoplasmic reticulum Source: TAIR
    4. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    5. extracellular region Source: UniProtKB-KW
    6. mitochondrion Source: TAIR
    7. nucleus Source: UniProtKB-SubCell
    8. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Cell wall, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, Vacuole

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype, probably due to functional redundancy.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 597572Protein disulfide isomerase-like 1-4PRO_0000400019Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi132 ↔ 135Redox-activePROSITE-ProRule annotation
    Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi471 ↔ 474Redox-activePROSITE-ProRule annotation
    Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9FF55.
    PRIDEiQ9FF55.

    Expressioni

    Tissue specificityi

    Expressed in germinating seedling, including the cotyledons and hypocotyl, in vascular tissues, in pollen grains, root tips, leaf trichomes, developing seeds and siliques.2 Publications

    Gene expression databases

    GenevestigatoriQ9FF55.

    Interactioni

    Subunit structurei

    Interacts with MEE8 and MED37A.1 Publication

    Protein-protein interaction databases

    BioGridi21429. 10 interactions.
    STRINGi3702.AT5G60640.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FF55.
    SMRiQ9FF55. Positions 5-550.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini85 – 208124Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini429 – 550122Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi594 – 5974Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG0526.
    HOGENOMiHOG000162459.
    InParanoidiQ9FF55.
    KOiK09580.
    OMAiKHATIPF.
    PhylomeDBiQ9FF55.

    Family and domain databases

    Gene3Di3.40.30.10. 4 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 4 hits.
    TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FF55-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAFRVLLLFS LTALLIFSAV SPSFAASSSD DVDDEDLSFL EDLKEDDVPG    50
    ADSLSSSTGF DEFEGGEEED PDMYNDDDDE EGDFSDLGNP DSDPLPTPEI 100
    DEKDVVVIKE RNFTDVIENN QYVLVEFYAP WCGHCQSLAP EYAAAATELK 150
    EDGVVLAKID ATEENELAQE YRVQGFPTLL FFVDGEHKPY TGGRTKETIV 200
    TWVKKKIGPG VYNLTTLDDA EKVLTSGNKV VLGYLNSLVG VEHDQLNAAS 250
    KAEDDVNFYQ TVNPDVAKMF HLDPESKRPA LVLVKKEEEK ISHFDGEFVK 300
    SALVSFVSAN KLALVSVFTR ETAPEIFESA IKKQLLLFVT KNESEKVLTE 350
    FQEAAKSFKG KLIFVSVDLD NEDYGKPVAE YFGVSGNGPK LIGYTGNEDP 400
    KKYFFDGEIQ SDKIKIFGED FLNDKLKPFY KSDPIPEKND EDVKIVVGDN 450
    FDEIVLDDSK DVLLEVYAPW CGHCQALEPM YNKLAKHLRS IDSLVITKMD 500
    GTTNEHPKAK AEGFPTILFF PAGNKTSEPI TVDTDRTVVA FYKFLRKHAT 550
    IPFKLEKPAS TESPKTAEST PKVETTETKE SPDSTTKSSQ SDSKDEL 597
    Length:597
    Mass (Da):66,357
    Last modified:March 1, 2001 - v1
    Checksum:i5A8FC7E72AA64B2B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti400 – 4001P → H in AAM65262. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005246 Genomic DNA. Translation: BAB09837.1.
    CP002688 Genomic DNA. Translation: AED97360.1.
    BT001994 mRNA. Translation: AAN72005.1.
    BT008359 mRNA. Translation: AAP37718.1.
    AY087725 mRNA. Translation: AAM65262.1.
    RefSeqiNP_851234.1. NM_180903.3. [Q9FF55-1]
    UniGeneiAt.22422.

    Genome annotation databases

    EnsemblPlantsiAT5G60640.1; AT5G60640.1; AT5G60640. [Q9FF55-1]
    GeneIDi836185.
    KEGGiath:AT5G60640.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005246 Genomic DNA. Translation: BAB09837.1 .
    CP002688 Genomic DNA. Translation: AED97360.1 .
    BT001994 mRNA. Translation: AAN72005.1 .
    BT008359 mRNA. Translation: AAP37718.1 .
    AY087725 mRNA. Translation: AAM65262.1 .
    RefSeqi NP_851234.1. NM_180903.3. [Q9FF55-1 ]
    UniGenei At.22422.

    3D structure databases

    ProteinModelPortali Q9FF55.
    SMRi Q9FF55. Positions 5-550.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21429. 10 interactions.
    STRINGi 3702.AT5G60640.1-P.

    Proteomic databases

    PaxDbi Q9FF55.
    PRIDEi Q9FF55.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G60640.1 ; AT5G60640.1 ; AT5G60640 . [Q9FF55-1 ]
    GeneIDi 836185.
    KEGGi ath:AT5G60640.

    Organism-specific databases

    TAIRi AT5G60640.

    Phylogenomic databases

    eggNOGi COG0526.
    HOGENOMi HOG000162459.
    InParanoidi Q9FF55.
    KOi K09580.
    OMAi KHATIPF.
    PhylomeDBi Q9FF55.

    Enzyme and pathway databases

    BioCyci ARA:AT5G60640-MONOMER.
    ARA:GQT-84-MONOMER.
    ARA:GQT-85-MONOMER.

    Gene expression databases

    Genevestigatori Q9FF55.

    Family and domain databases

    Gene3Di 3.40.30.10. 4 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 4 hits.
    TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
      Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
      Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
      Lu D.-P., Christopher D.A.
      Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
      d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
      BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor."
      Cho E.J., Yuen C.Y., Kang B.H., Ondzighi C.A., Staehelin L.A., Christopher D.A.
      Mol. Cells 32:459-475(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MEE8 AND MED37A/BIP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPDI14_ARATH
    AccessioniPrimary (citable) accession number: Q9FF55
    Secondary accession number(s): Q8LAM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3