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Q9FF55

- PDI14_ARATH

UniProt

Q9FF55 - PDI14_ARATH

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Protein
Protein disulfide isomerase-like 1-4
Gene
PDIL1-4, PDI2, PDIL2-2, At5g60640, MUP24.6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei132 – 1321Nucleophile By similarity
Sitei133 – 1331Contributes to redox potential value By similarity
Sitei134 – 1341Contributes to redox potential value By similarity
Active sitei135 – 1351Nucleophile By similarity
Sitei194 – 1941Lowers pKa of C-terminal Cys of first active site By similarity
Active sitei471 – 4711Nucleophile By similarity
Sitei472 – 4721Contributes to redox potential value By similarity
Sitei473 – 4731Contributes to redox potential value By similarity
Active sitei474 – 4741Nucleophile By similarity
Sitei536 – 5361Lowers pKa of C-terminal Cys of second active site By similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. protein folding Source: GOC
  3. response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT5G60640-MONOMER.
ARA:GQT-84-MONOMER.
ARA:GQT-85-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide isomerase-like 1-4 (EC:5.3.4.1)
Short name:
AtPDIL1-4
Alternative name(s):
Protein disulfide isomerase 2
Short name:
AtPDI2
Protein disulfide isomerase-like 2-2
Short name:
AtPDIL2-2
Gene namesi
Name:PDIL1-4
Synonyms:PDI2, PDIL2-2
Ordered Locus Names:At5g60640
ORF Names:MUP24.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G60640.

Subcellular locationi

Endoplasmic reticulum lumen. Golgi apparatus. Vacuole. Nucleus. Secretedcell wall 1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. chloroplast Source: TAIR
  3. endoplasmic reticulum Source: TAIR
  4. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  5. extracellular region Source: UniProtKB-KW
  6. mitochondrion Source: TAIR
  7. nucleus Source: UniProtKB-SubCell
  8. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, probably due to functional redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 597572Protein disulfide isomerase-like 1-4
PRO_0000400019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi112 – 1121N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi132 ↔ 135Redox-active By similarity
Glycosylationi213 – 2131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi342 – 3421N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi471 ↔ 474Redox-active By similarity
Glycosylationi524 – 5241N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FF55.
PRIDEiQ9FF55.

Expressioni

Tissue specificityi

Expressed in germinating seedling, including the cotyledons and hypocotyl, in vascular tissues, in pollen grains, root tips, leaf trichomes, developing seeds and siliques.2 Publications

Gene expression databases

GenevestigatoriQ9FF55.

Interactioni

Subunit structurei

Interacts with MEE8 and MED37A.1 Publication

Protein-protein interaction databases

BioGridi21429. 10 interactions.
STRINGi3702.AT5G60640.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FF55.
SMRiQ9FF55. Positions 5-550.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 208124Thioredoxin 1
Add
BLAST
Domaini429 – 550122Thioredoxin 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi594 – 5974Prevents secretion from ER By similarity

Sequence similaritiesi

Contains 2 thioredoxin domains.

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiCOG0526.
HOGENOMiHOG000162459.
InParanoidiQ9FF55.
KOiK09580.
OMAiKHATIPF.
PhylomeDBiQ9FF55.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FF55-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAFRVLLLFS LTALLIFSAV SPSFAASSSD DVDDEDLSFL EDLKEDDVPG    50
ADSLSSSTGF DEFEGGEEED PDMYNDDDDE EGDFSDLGNP DSDPLPTPEI 100
DEKDVVVIKE RNFTDVIENN QYVLVEFYAP WCGHCQSLAP EYAAAATELK 150
EDGVVLAKID ATEENELAQE YRVQGFPTLL FFVDGEHKPY TGGRTKETIV 200
TWVKKKIGPG VYNLTTLDDA EKVLTSGNKV VLGYLNSLVG VEHDQLNAAS 250
KAEDDVNFYQ TVNPDVAKMF HLDPESKRPA LVLVKKEEEK ISHFDGEFVK 300
SALVSFVSAN KLALVSVFTR ETAPEIFESA IKKQLLLFVT KNESEKVLTE 350
FQEAAKSFKG KLIFVSVDLD NEDYGKPVAE YFGVSGNGPK LIGYTGNEDP 400
KKYFFDGEIQ SDKIKIFGED FLNDKLKPFY KSDPIPEKND EDVKIVVGDN 450
FDEIVLDDSK DVLLEVYAPW CGHCQALEPM YNKLAKHLRS IDSLVITKMD 500
GTTNEHPKAK AEGFPTILFF PAGNKTSEPI TVDTDRTVVA FYKFLRKHAT 550
IPFKLEKPAS TESPKTAEST PKVETTETKE SPDSTTKSSQ SDSKDEL 597
Length:597
Mass (Da):66,357
Last modified:March 1, 2001 - v1
Checksum:i5A8FC7E72AA64B2B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti400 – 4001P → H in AAM65262. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB005246 Genomic DNA. Translation: BAB09837.1.
CP002688 Genomic DNA. Translation: AED97360.1.
BT001994 mRNA. Translation: AAN72005.1.
BT008359 mRNA. Translation: AAP37718.1.
AY087725 mRNA. Translation: AAM65262.1.
RefSeqiNP_851234.1. NM_180903.3. [Q9FF55-1]
UniGeneiAt.22422.

Genome annotation databases

EnsemblPlantsiAT5G60640.1; AT5G60640.1; AT5G60640. [Q9FF55-1]
GeneIDi836185.
KEGGiath:AT5G60640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB005246 Genomic DNA. Translation: BAB09837.1 .
CP002688 Genomic DNA. Translation: AED97360.1 .
BT001994 mRNA. Translation: AAN72005.1 .
BT008359 mRNA. Translation: AAP37718.1 .
AY087725 mRNA. Translation: AAM65262.1 .
RefSeqi NP_851234.1. NM_180903.3. [Q9FF55-1 ]
UniGenei At.22422.

3D structure databases

ProteinModelPortali Q9FF55.
SMRi Q9FF55. Positions 5-550.
ModBasei Search...

Protein-protein interaction databases

BioGridi 21429. 10 interactions.
STRINGi 3702.AT5G60640.1-P.

Proteomic databases

PaxDbi Q9FF55.
PRIDEi Q9FF55.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G60640.1 ; AT5G60640.1 ; AT5G60640 . [Q9FF55-1 ]
GeneIDi 836185.
KEGGi ath:AT5G60640.

Organism-specific databases

TAIRi AT5G60640.

Phylogenomic databases

eggNOGi COG0526.
HOGENOMi HOG000162459.
InParanoidi Q9FF55.
KOi K09580.
OMAi KHATIPF.
PhylomeDBi Q9FF55.

Enzyme and pathway databases

BioCyci ARA:AT5G60640-MONOMER.
ARA:GQT-84-MONOMER.
ARA:GQT-85-MONOMER.

Gene expression databases

Genevestigatori Q9FF55.

Family and domain databases

Gene3Di 3.40.30.10. 4 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
    Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
    Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
    Lu D.-P., Christopher D.A.
    Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
    d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
    BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor."
    Cho E.J., Yuen C.Y., Kang B.H., Ondzighi C.A., Staehelin L.A., Christopher D.A.
    Mol. Cells 32:459-475(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MEE8 AND MED37A/BIP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPDI14_ARATH
AccessioniPrimary (citable) accession number: Q9FF55
Secondary accession number(s): Q8LAM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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