Reviewed, UniProtKB/Swiss-Prot Q9FF10 (HPSE1_ARATH)

Names and origin

Protein names

Recommended name:
    Heparanase-like protein 1
    EC=3.2.-.-

Gene names

Ordered Locus Names:	At5g07830
ORF Names:	F13G24.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans By similarity.
Subcellular location	Lysosome membrane; Peripheral membrane protein By similarity. Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 79 family.

Ontologies

Keywords
Cellular component	Lysosome Membrane Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 543	519	Heparanase-like protein 1		PRO_0000042269
Sites
	Active site	201	1	Proton donor Potential
	Active site	320	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential
	Glycosylation	428	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FF10-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0FA2248948282FF6	FASTA	543	60,250
	10 20 30 40 50 60 MGFRVCVIVV FLGCLLLVPE KTMAQEMKRA SIVIQGARRV CETDENFVCA TLDWWPHDKC 70 80 90 100 110 120 NYDQCPWGYS SVINMDLTRP LLTKAIKAFK PLRIRIGGSL QDQVIYDVGN LKTPCRPFQK 130 140 150 160 170 180 MNSGLFGFSK GCLHMKRWDE LNSFLTATGA VVTFGLNALR GRHKLRGKAW GGAWDHINTQ 190 200 210 220 230 240 DFLNYTVSKG YVIDSWEFGN ELSGSGVGAS VSAELYGKDL IVLKDVINKV YKNSWLHKPI 250 260 270 280 290 300 LVAPGGFYEQ QWYTKLLEIS GPSVVDVVTH HIYNLGSGND PALVKKIMDP SYLSQVSKTF 310 320 330 340 350 360 KDVNQTIQEH GPWASPWVGE SGGAYNSGGR HVSDTFIDSF WYLDQLGMSA RHNTKVYCRQ 370 380 390 400 410 420 TLVGGFYGLL EKGTFVPNPD YYSALLWHRL MGKGVLAVQT DGPPQLRVYA HCSKGRAGVT 430 440 450 460 470 480 LLLINLSNQS DFTVSVSNGI NVVLNAESRK KKSLLDTLKR PFSWIGSKAS DGYLNREEYH 490 500 510 520 530 540 LTPENGVLRS KTMVLNGKSL KPTATGDIPS LEPVLRSVNS PLNVLPLSMS FIVLPNFDAS ACS

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB005249 Genomic DNA. Translation: BAB09947.1. AL133421 Genomic DNA. Translation: CAB62595.1. Different initiation. BT015345 mRNA. Translation: AAU05468.1. BT015800 mRNA. Translation: AAU93572.1.
IPI	IPI00521127.
PIR	T45608.
RefSeq	NP_196400.2.
UniGene	At.48994
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH79. Glycoside Hydrolase Family 79.
Proteomic databases
PRIDE	Q9FF10.
Genome annotation databases
GeneID	830676.
GenomeReviews	Gene locus AT5G07830 in contig BA000015_GR.
KEGG	ath:AT5G07830.
NMPDR	fig|3702.1.peg.22924.
Organism-specific databases
TAIR	At5g07830.
Phylogenomic databases
OMA	Q9FF10. VAPGGFY.
Gene expression databases
GermOnline	AT5G07830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005199. Glyco_hydro_79_N. [Graphical view]
PANTHER	PTHR14363. Glyco_hydro_79_N. 1 hit.
Pfam	PF03662. Glyco_hydro_79n. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPSE1_ARATH

Accession

Primary (citable) accession number: Q9FF10
Secondary accession number(s): Q9SDA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)