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Protein

12-oxophytodienoate reductase 3

Gene

OPR3

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. It is required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program (By similarity). In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively.By similarity

Catalytic activityi

8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH.

Cofactori

FMNBy similarity

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64FMN; via amide nitrogen2 Publications1
Binding sitei106FMN2 Publications1
Active sitei190Proton donorBy similarity1
Binding sitei237FMN2 Publications1
Binding sitei283Substrate; via amide nitrogenBy similarity1
Binding sitei321FMN; via amide nitrogen2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 33FMN2 Publications3
Nucleotide bindingi342 – 343FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Flavoprotein, FMN, NADP

Enzyme and pathway databases

BRENDAi1.3.1.42. 3101.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
12-oxophytodienoate reductase 3 (EC:1.3.1.42)
Alternative name(s):
12-oxophytodienoate-10,11-reductase 3
Short name:
OPDA-reductase 3
LeOPR3
Gene namesi
Name:OPR3
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944891 – 39612-oxophytodienoate reductase 3Add BLAST396

Proteomic databases

PaxDbiQ9FEW9.
PRIDEiQ9FEW9.

Expressioni

Tissue specificityi

Expressed in roots and to a lower extent in leaves and flowers.

Inductioni

By wounding, locally and systemically.

Interactioni

Protein-protein interaction databases

DIPiDIP-61271N.
STRINGi4081.Solyc07g007870.2.1.

Structurei

Secondary structure

1396
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 13Combined sources3
Beta strandi16 – 18Combined sources3
Beta strandi21 – 24Combined sources4
Beta strandi26 – 29Combined sources4
Helixi38 – 40Combined sources3
Helixi44 – 53Combined sources10
Beta strandi59 – 61Combined sources3
Beta strandi65 – 68Combined sources4
Beta strandi74 – 76Combined sources3
Helixi83 – 98Combined sources16
Beta strandi102 – 108Combined sources7
Helixi116 – 118Combined sources3
Helixi120 – 122Combined sources3
Beta strandi126 – 128Combined sources3
Beta strandi138 – 140Combined sources3
Beta strandi146 – 148Combined sources3
Helixi157 – 159Combined sources3
Helixi160 – 176Combined sources17
Beta strandi180 – 185Combined sources6
Helixi191 – 196Combined sources6
Turni198 – 200Combined sources3
Beta strandi208 – 210Combined sources3
Helixi211 – 229Combined sources19
Helixi231 – 233Combined sources3
Beta strandi234 – 238Combined sources5
Helixi244 – 246Combined sources3
Helixi252 – 270Combined sources19
Beta strandi275 – 280Combined sources6
Turni286 – 289Combined sources4
Beta strandi290 – 292Combined sources3
Turni293 – 296Combined sources4
Helixi297 – 311Combined sources15
Beta strandi312 – 314Combined sources3
Beta strandi316 – 321Combined sources6
Helixi324 – 332Combined sources9
Beta strandi337 – 342Combined sources6
Helixi343 – 347Combined sources5
Helixi351 – 357Combined sources7
Helixi366 – 368Combined sources3
Turni376 – 378Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HS6X-ray1.90A/B1-396[»]
2HS8X-ray1.90A/B1-396[»]
2HSAX-ray1.50A/B1-396[»]
3HGOX-ray2.30A/B1-396[»]
3HGSX-ray2.00A/B1-396[»]
ProteinModelPortaliQ9FEW9.
SMRiQ9FEW9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9FEW9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 188Substrate-bindingBy similarity4
Regioni342 – 343FMN2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi394 – 396Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0134. Eukaryota.
COG1902. LUCA.
InParanoidiQ9FEW9.
KOiK05894.
OMAiHDKGGHI.
OrthoDBiEOG09360FPQ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FEW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSAQDGNN PLFSPYKMGK FNLSHRVVLA PMTRCRALNN IPQAALGEYY
60 70 80 90 100
EQRATAGGFL ITEGTMISPT SAGFPHVPGI FTKEQVREWK KIVDVVHAKG
110 120 130 140 150
AVIFCQLWHV GRASHEVYQP AGAAPISSTE KPISNRWRIL MPDGTHGIYP
160 170 180 190 200
KPRAIGTYEI SQVVEDYRRS ALNAIEAGFD GIEIHGAHGY LIDQFLKDGI
210 220 230 240 250
NDRTDEYGGS LANRCKFITQ VVQAVVSAIG ADRVGVRVSP AIDHLDAMDS
260 270 280 290 300
NPLSLGLAVV ERLNKIQLHS GSKLAYLHVT QPRYVAYGQT EAGRLGSEEE
310 320 330 340 350
EARLMRTLRN AYQGTFICSG GYTRELGIEA VAQGDADLVS YGRLFISNPD
360 370 380 390
LVMRIKLNAP LNKYNRKTFY TQDPVVGYTD YPFLQGNGSN GPLSRL
Length:396
Mass (Da):43,520
Last modified:March 1, 2001 - v1
Checksum:iC9550E54D2EAF75B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278332 mRNA. Translation: CAC21424.1.
RefSeqiNP_001233873.1. NM_001246944.2.
UniGeneiLes.3762.

Genome annotation databases

EnsemblPlantsiSolyc07g007870.2.1; Solyc07g007870.2.1; Solyc07g007870.2.
GeneIDi543763.
GrameneiSolyc07g007870.2.1; Solyc07g007870.2.1; Solyc07g007870.2.
KEGGisly:543763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278332 mRNA. Translation: CAC21424.1.
RefSeqiNP_001233873.1. NM_001246944.2.
UniGeneiLes.3762.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HS6X-ray1.90A/B1-396[»]
2HS8X-ray1.90A/B1-396[»]
2HSAX-ray1.50A/B1-396[»]
3HGOX-ray2.30A/B1-396[»]
3HGSX-ray2.00A/B1-396[»]
ProteinModelPortaliQ9FEW9.
SMRiQ9FEW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61271N.
STRINGi4081.Solyc07g007870.2.1.

Proteomic databases

PaxDbiQ9FEW9.
PRIDEiQ9FEW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc07g007870.2.1; Solyc07g007870.2.1; Solyc07g007870.2.
GeneIDi543763.
GrameneiSolyc07g007870.2.1; Solyc07g007870.2.1; Solyc07g007870.2.
KEGGisly:543763.

Phylogenomic databases

eggNOGiKOG0134. Eukaryota.
COG1902. LUCA.
InParanoidiQ9FEW9.
KOiK05894.
OMAiHDKGGHI.
OrthoDBiEOG09360FPQ.

Enzyme and pathway databases

UniPathwayiUPA00382.
BRENDAi1.3.1.42. 3101.

Miscellaneous databases

EvolutionaryTraceiQ9FEW9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPR3_SOLLC
AccessioniPrimary (citable) accession number: Q9FEW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.