Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidinol-phosphate aminotransferase, chloroplastic

Gene

HPA

Organism
Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate.

Cofactori

Pathwayi: L-histidine biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. Histidinol-phosphate aminotransferase, chloroplastic (HPA)
  8. no protein annotated in this organism
  9. no protein annotated in this organism
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00031; UER00012.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol-phosphate aminotransferase, chloroplastic (EC:2.6.1.9)
Alternative name(s):
Imidazole acetol-phosphate transaminase
Gene namesi
Name:HPA
OrganismiNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifieri4092 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi381R → H in His1-; no histidine biosynthesis. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35ChloroplastSequence analysisAdd BLAST35
ChainiPRO_000001344836 – 413Histidinol-phosphate aminotransferase, chloroplasticAdd BLAST378

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273N6-(pyridoxal phosphate)lysineBy similarity1

Expressioni

Tissue specificityi

Expressed in flowers, leaves, stems and roots.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9FEW2.
SMRiQ9FEW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01023. HisC_aminotrans_2. 1 hit.
InterProiView protein in InterPro
IPR004839. Aminotransferase_I/II.
IPR005861. HisP_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_sub2.
PfamiView protein in Pfam
PF00155. Aminotran_1_2. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01141. hisC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FEW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVIELCNTS SICIGRANPS CCSIERNQRR RIICMASSVP VQEESQQKQR
60 70 80 90 100
VTGDAFIRPH LLKLSPYQPI LPFEVLSTRL GRKPEDIVKL DANENPYGPP
110 120 130 140 150
PEVIEALGAM KFPYIYPDPE SRTLRAALAE DSGLESEYIL AGCGADELID
160 170 180 190 200
LIMRCVLDPG DMIVDCPPTF TMYEFDAAVN GAHVIKVPRN PDFSLDVERI
210 220 230 240 250
AEVVEHEKPK CIFLTSPNNP DGSIIDDETL LKILDLPILV ILDEAYVEFS
260 270 280 290 300
GMESKMKWVK KHENLIVLRT FSKRAGLAGL RVGYGAFPKS IIEFLWRAKQ
310 320 330 340 350
PYNVSVAAEV AACAALKNPA YLENVKVALV QERERLFNLL KEVPFLDPYP
360 370 380 390 400
SYSNFILCKV TSGMDAKKLK EDLATMGVMI RHYNSKELKG YVRVSVGKPE
410
HTEALMKCLK HFY
Length:413
Mass (Da):46,299
Last modified:March 1, 2001 - v1
Checksum:i587DAFE3D440A5AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278767 mRNA. Translation: CAC20728.1.

Similar proteinsi

Entry informationi

Entry nameiHIS8_NICPL
AccessioniPrimary (citable) accession number: Q9FEW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2001
Last modified: April 12, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families