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Q9FEA2

- SYE_ARATH

UniProt

Q9FEA2 - SYE_ARATH

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Protein
Glutamate--tRNA ligase, chloroplastic/mitochondrial
Gene
At5g64050, MHJ24.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671ATP By similarity
Binding sitei93 – 931Glutamate By similarity
Binding sitei263 – 2631Glutamate By similarity
Binding sitei266 – 2661ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 3055ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: UniProtKB-EC
  3. protein binding Source: TAIR
  4. tRNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. chloroplast organization Source: TAIR
  2. glutamyl-tRNA aminoacylation Source: TAIR
  3. mitochondrion organization Source: TAIR
  4. ovule development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G64050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, chloroplastic/mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Ordered Locus Names:At5g64050
ORF Names:MHJ24.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G64050.

Subcellular locationi

Plastidchloroplast. Mitochondrion Reviewed prediction 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 570Glutamate--tRNA ligase, chloroplastic/mitochondrialUniRule annotationPRO_0000119735
Transit peptidei1 – ?Chloroplast and mitochondrion Reviewed prediction

Proteomic databases

PaxDbiQ9FEA2.
PRIDEiQ9FEA2.

Expressioni

Gene expression databases

GenevestigatoriQ9FEA2.

Interactioni

Protein-protein interaction databases

IntActiQ9FEA2. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9FEA2.
SMRiQ9FEA2. Positions 54-523.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 593Glutamate binding By similarity
Regioni245 – 2495Glutamate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi301 – 3055"KMSKS" regionUniRule annotation

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
InParanoidiQ9FEA2.
KOiK01885.
OMAiKHYDGDF.
PhylomeDBiQ9FEA2.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FEA2-1 [UniParc]FASTAAdd to Basket

« Hide

MASLVYGTPW LRVRSLPELA PAFLRRRQSS LFYCSRRSFA VVACSTPVNN    50
GGSVRVRFAP SPTGNLHVGG ARTALFNYLF ARSKGGKFVL RIEDTDLERS 100
TRESEAAVLQ DLSWLGLDWD EGPGVSGDFG PYRQSERNAL YKQYAEKLLE 150
SGHVYRCFCS SEELVKMKEN AKLKQLPPVY TGKWATASDA EIEQELEKGT 200
PFTYRFRVPK EGSLKINDLI RGEVCWNLDT LGDFVVMRSN GQPVYNFCVT 250
VDDATMAISH VIRAEEHLPN TLRQALIYKA LKFPMPQFAH VSLILAPDRS 300
KLSKRHGATS VGQYREMGYL PQGMVNYLAL LGWGDGTENE FFTLEDLVEK 350
FSIERVNKSG AIFDSTKLRW MNGQHLRALP NEKLTKLVGE RWKSAGILTE 400
SEGSFVNEAV ELLKDGIELV TDSDKVLLNL LSYPLHATLA SPEAKPAVED 450
KLHEVAASLI AAYDSGEIPS ALEEGQGAWQ KWVKAFGKSL KRKGKSLFMP 500
LRVLLTGKLH GPEMGTSIVL IYKAGSPGIV VPQAGFVSME ERFKILREID 550
WEALNKDESV PLESTATVST 570
Length:570
Mass (Da):63,466
Last modified:March 1, 2001 - v1
Checksum:i5EA70E3595F53587
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741A → V in AAL06875. 1 Publication
Sequence conflicti74 – 741A → V in AAL32638. 1 Publication
Sequence conflicti74 – 741A → V in AAM47995. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF241841 mRNA. Translation: AAG29098.1.
AB008266 Genomic DNA. Translation: BAB10273.1.
CP002688 Genomic DNA. Translation: AED97834.1.
AY054215 mRNA. Translation: AAL06875.1.
AY062560 mRNA. Translation: AAL32638.1.
AY114676 mRNA. Translation: AAM47995.1.
RefSeqiNP_201210.1. NM_125801.3.
UniGeneiAt.8457.

Genome annotation databases

EnsemblPlantsiAT5G64050.1; AT5G64050.1; AT5G64050.
GeneIDi836526.
KEGGiath:AT5G64050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF241841 mRNA. Translation: AAG29098.1 .
AB008266 Genomic DNA. Translation: BAB10273.1 .
CP002688 Genomic DNA. Translation: AED97834.1 .
AY054215 mRNA. Translation: AAL06875.1 .
AY062560 mRNA. Translation: AAL32638.1 .
AY114676 mRNA. Translation: AAM47995.1 .
RefSeqi NP_201210.1. NM_125801.3.
UniGenei At.8457.

3D structure databases

ProteinModelPortali Q9FEA2.
SMRi Q9FEA2. Positions 54-523.
ModBasei Search...

Protein-protein interaction databases

IntActi Q9FEA2. 1 interaction.

Proteomic databases

PaxDbi Q9FEA2.
PRIDEi Q9FEA2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G64050.1 ; AT5G64050.1 ; AT5G64050 .
GeneIDi 836526.
KEGGi ath:AT5G64050.

Organism-specific databases

GeneFarmi 2420.
TAIRi AT5G64050.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
InParanoidi Q9FEA2.
KOi K01885.
OMAi KHYDGDF.
PhylomeDBi Q9FEA2.

Enzyme and pathway databases

BioCyci ARA:AT5G64050-MONOMER.

Gene expression databases

Genevestigatori Q9FEA2.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of Arabidopsis thaliana Glu-tRNA(Glu) synthetase mRNA."
    Chang W., Soell D.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYE_ARATH
AccessioniPrimary (citable) accession number: Q9FEA2
Secondary accession number(s): Q940P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi