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Protein

Glutamate--tRNA ligase, chloroplastic/mitochondrial

Gene

OVA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671ATPBy similarity
Binding sitei93 – 931GlutamateBy similarity
Binding sitei263 – 2631GlutamateBy similarity
Binding sitei266 – 2661ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 3055ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • chloroplast organization Source: TAIR
  • glutamyl-tRNA aminoacylation Source: TAIR
  • mitochondrion organization Source: TAIR
  • ovule development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G64050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.17Curated)
Alternative name(s):
AtERS1 Publication
Glutamyl-tRNA synthetaseCurated
Short name:
GluRSCurated
Protein OVULE ABORTION 31 Publication
Gene namesi
Name:OVA31 Publication
Ordered Locus Names:At5g64050
ORF Names:MHJ24.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G64050.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal. In heterozygous plants, aborted ovules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 570Glutamate--tRNA ligase, chloroplastic/mitochondrialCuratedPRO_0000119735
Transit peptidei1 – ?Chloroplast and mitochondrionCurated

Proteomic databases

PaxDbiQ9FEA2.
PRIDEiQ9FEA2.

Interactioni

Protein-protein interaction databases

BioGridi21768. 1 interaction.
IntActiQ9FEA2. 1 interaction.
STRINGi3702.AT5G64050.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FEA2.
SMRiQ9FEA2. Positions 54-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 593Glutamate bindingBy similarity
Regioni245 – 2495Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionCuratedAdd
BLAST
Motifi301 – 3055"KMSKS" regionCurated

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
InParanoidiQ9FEA2.
KOiK01885.
OMAiSYPLHAT.
PhylomeDBiQ9FEA2.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FEA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLVYGTPW LRVRSLPELA PAFLRRRQSS LFYCSRRSFA VVACSTPVNN
60 70 80 90 100
GGSVRVRFAP SPTGNLHVGG ARTALFNYLF ARSKGGKFVL RIEDTDLERS
110 120 130 140 150
TRESEAAVLQ DLSWLGLDWD EGPGVSGDFG PYRQSERNAL YKQYAEKLLE
160 170 180 190 200
SGHVYRCFCS SEELVKMKEN AKLKQLPPVY TGKWATASDA EIEQELEKGT
210 220 230 240 250
PFTYRFRVPK EGSLKINDLI RGEVCWNLDT LGDFVVMRSN GQPVYNFCVT
260 270 280 290 300
VDDATMAISH VIRAEEHLPN TLRQALIYKA LKFPMPQFAH VSLILAPDRS
310 320 330 340 350
KLSKRHGATS VGQYREMGYL PQGMVNYLAL LGWGDGTENE FFTLEDLVEK
360 370 380 390 400
FSIERVNKSG AIFDSTKLRW MNGQHLRALP NEKLTKLVGE RWKSAGILTE
410 420 430 440 450
SEGSFVNEAV ELLKDGIELV TDSDKVLLNL LSYPLHATLA SPEAKPAVED
460 470 480 490 500
KLHEVAASLI AAYDSGEIPS ALEEGQGAWQ KWVKAFGKSL KRKGKSLFMP
510 520 530 540 550
LRVLLTGKLH GPEMGTSIVL IYKAGSPGIV VPQAGFVSME ERFKILREID
560 570
WEALNKDESV PLESTATVST
Length:570
Mass (Da):63,466
Last modified:March 1, 2001 - v1
Checksum:i5EA70E3595F53587
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741A → V in AAL06875 (PubMed:14593172).Curated
Sequence conflicti74 – 741A → V in AAL32638 (PubMed:14593172).Curated
Sequence conflicti74 – 741A → V in AAM47995 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241841 mRNA. Translation: AAG29098.1.
AB008266 Genomic DNA. Translation: BAB10273.1.
CP002688 Genomic DNA. Translation: AED97834.1.
AY054215 mRNA. Translation: AAL06875.1.
AY062560 mRNA. Translation: AAL32638.1.
AY114676 mRNA. Translation: AAM47995.1.
RefSeqiNP_201210.1. NM_125801.3.
UniGeneiAt.8457.

Genome annotation databases

EnsemblPlantsiAT5G64050.1; AT5G64050.1; AT5G64050.
GeneIDi836526.
KEGGiath:AT5G64050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241841 mRNA. Translation: AAG29098.1.
AB008266 Genomic DNA. Translation: BAB10273.1.
CP002688 Genomic DNA. Translation: AED97834.1.
AY054215 mRNA. Translation: AAL06875.1.
AY062560 mRNA. Translation: AAL32638.1.
AY114676 mRNA. Translation: AAM47995.1.
RefSeqiNP_201210.1. NM_125801.3.
UniGeneiAt.8457.

3D structure databases

ProteinModelPortaliQ9FEA2.
SMRiQ9FEA2. Positions 54-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21768. 1 interaction.
IntActiQ9FEA2. 1 interaction.
STRINGi3702.AT5G64050.1.

Proteomic databases

PaxDbiQ9FEA2.
PRIDEiQ9FEA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G64050.1; AT5G64050.1; AT5G64050.
GeneIDi836526.
KEGGiath:AT5G64050.

Organism-specific databases

GeneFarmi2420.
TAIRiAT5G64050.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
InParanoidiQ9FEA2.
KOiK01885.
OMAiSYPLHAT.
PhylomeDBiQ9FEA2.

Enzyme and pathway databases

BioCyciARA:AT5G64050-MONOMER.

Miscellaneous databases

PROiQ9FEA2.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of Arabidopsis thaliana Glu-tRNA(Glu) synthetase mRNA."
    Chang W., Soell D.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Inactivation of organellar glutamyl- and seryl-tRNA synthetases leads to developmental arrest of chloroplasts and mitochondria in higher plants."
    Kim Y.K., Lee J.Y., Cho H.S., Lee S.S., Ha H.J., Kim S., Choi D., Pai H.S.
    J. Biol. Chem. 280:37098-37106(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Requirement of aminoacyl-tRNA synthetases for gametogenesis and embryo development in Arabidopsis."
    Berg M., Rogers R., Muralla R., Meinke D.
    Plant J. 44:866-878(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in Arabidopsis thaliana."
    Duchene A.-M., Giritch A., Hoffmann B., Cognat V., Lancelin D., Peeters N.M., Zaepfel M., Marechal-Drouard L., Small I.D.
    Proc. Natl. Acad. Sci. U.S.A. 102:16484-16489(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "How can organellar protein N-terminal sequences be dual targeting signals? In silico analysis and mutagenesis approach."
    Pujol C., Marechal-Drouard L., Duchene A.M.
    J. Mol. Biol. 369:356-367(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYEM_ARATH
AccessioniPrimary (citable) accession number: Q9FEA2
Secondary accession number(s): Q940P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: July 22, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.