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Q9FEA2

- SYE_ARATH

UniProt

Q9FEA2 - SYE_ARATH

Protein

Glutamate--tRNA ligase, chloroplastic/mitochondrial

Gene

At5g64050

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei67 – 671ATPBy similarity
    Binding sitei93 – 931GlutamateBy similarity
    Binding sitei263 – 2631GlutamateBy similarity
    Binding sitei266 – 2661ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi301 – 3055ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: UniProtKB-EC
    3. protein binding Source: TAIR
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. chloroplast organization Source: TAIR
    2. glutamyl-tRNA aminoacylation Source: TAIR
    3. mitochondrion organization Source: TAIR
    4. ovule development Source: TAIR

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G64050-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase, chloroplastic/mitochondrial (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    Ordered Locus Names:At5g64050
    ORF Names:MHJ24.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G64050.

    Subcellular locationi

    Plastidchloroplast 1 Publication. Mitochondrion Curated

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 570Glutamate--tRNA ligase, chloroplastic/mitochondrialPRO_0000119735
    Transit peptidei1 – ?Chloroplast and mitochondrionSequence Analysis

    Proteomic databases

    PaxDbiQ9FEA2.
    PRIDEiQ9FEA2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FEA2.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9FEA2. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FEA2.
    SMRiQ9FEA2. Positions 54-523.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni57 – 593Glutamate bindingBy similarity
    Regioni245 – 2495Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi301 – 3055"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    InParanoidiQ9FEA2.
    KOiK01885.
    OMAiKHYDGDF.
    PhylomeDBiQ9FEA2.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FEA2-1 [UniParc]FASTAAdd to Basket

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    MASLVYGTPW LRVRSLPELA PAFLRRRQSS LFYCSRRSFA VVACSTPVNN    50
    GGSVRVRFAP SPTGNLHVGG ARTALFNYLF ARSKGGKFVL RIEDTDLERS 100
    TRESEAAVLQ DLSWLGLDWD EGPGVSGDFG PYRQSERNAL YKQYAEKLLE 150
    SGHVYRCFCS SEELVKMKEN AKLKQLPPVY TGKWATASDA EIEQELEKGT 200
    PFTYRFRVPK EGSLKINDLI RGEVCWNLDT LGDFVVMRSN GQPVYNFCVT 250
    VDDATMAISH VIRAEEHLPN TLRQALIYKA LKFPMPQFAH VSLILAPDRS 300
    KLSKRHGATS VGQYREMGYL PQGMVNYLAL LGWGDGTENE FFTLEDLVEK 350
    FSIERVNKSG AIFDSTKLRW MNGQHLRALP NEKLTKLVGE RWKSAGILTE 400
    SEGSFVNEAV ELLKDGIELV TDSDKVLLNL LSYPLHATLA SPEAKPAVED 450
    KLHEVAASLI AAYDSGEIPS ALEEGQGAWQ KWVKAFGKSL KRKGKSLFMP 500
    LRVLLTGKLH GPEMGTSIVL IYKAGSPGIV VPQAGFVSME ERFKILREID 550
    WEALNKDESV PLESTATVST 570
    Length:570
    Mass (Da):63,466
    Last modified:March 1, 2001 - v1
    Checksum:i5EA70E3595F53587
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741A → V in AAL06875. (PubMed:14593172)Curated
    Sequence conflicti74 – 741A → V in AAL32638. (PubMed:14593172)Curated
    Sequence conflicti74 – 741A → V in AAM47995. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241841 mRNA. Translation: AAG29098.1.
    AB008266 Genomic DNA. Translation: BAB10273.1.
    CP002688 Genomic DNA. Translation: AED97834.1.
    AY054215 mRNA. Translation: AAL06875.1.
    AY062560 mRNA. Translation: AAL32638.1.
    AY114676 mRNA. Translation: AAM47995.1.
    RefSeqiNP_201210.1. NM_125801.3.
    UniGeneiAt.8457.

    Genome annotation databases

    EnsemblPlantsiAT5G64050.1; AT5G64050.1; AT5G64050.
    GeneIDi836526.
    KEGGiath:AT5G64050.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241841 mRNA. Translation: AAG29098.1 .
    AB008266 Genomic DNA. Translation: BAB10273.1 .
    CP002688 Genomic DNA. Translation: AED97834.1 .
    AY054215 mRNA. Translation: AAL06875.1 .
    AY062560 mRNA. Translation: AAL32638.1 .
    AY114676 mRNA. Translation: AAM47995.1 .
    RefSeqi NP_201210.1. NM_125801.3.
    UniGenei At.8457.

    3D structure databases

    ProteinModelPortali Q9FEA2.
    SMRi Q9FEA2. Positions 54-523.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9FEA2. 1 interaction.

    Proteomic databases

    PaxDbi Q9FEA2.
    PRIDEi Q9FEA2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G64050.1 ; AT5G64050.1 ; AT5G64050 .
    GeneIDi 836526.
    KEGGi ath:AT5G64050.

    Organism-specific databases

    GeneFarmi 2420.
    TAIRi AT5G64050.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    InParanoidi Q9FEA2.
    KOi K01885.
    OMAi KHYDGDF.
    PhylomeDBi Q9FEA2.

    Enzyme and pathway databases

    BioCyci ARA:AT5G64050-MONOMER.

    Gene expression databases

    Genevestigatori Q9FEA2.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of Arabidopsis thaliana Glu-tRNA(Glu) synthetase mRNA."
      Chang W., Soell D.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYE_ARATH
    AccessioniPrimary (citable) accession number: Q9FEA2
    Secondary accession number(s): Q940P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3