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Protein

Eukaryotic translation initiation factor 2 subunit alpha

Gene

EIF2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-ATH-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-ATH-382556 ABC-family proteins mediated transport
R-ATH-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-ATH-72702 Ribosomal scanning and start codon recognition
R-ATH-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit alphaCurated
Short name:
eIF2-alphaCurated
Gene namesi
Name:EIF2ACurated
Ordered Locus Names:At5g05470Imported
ORF Names:K18I23.28Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G05470
TAIRilocus:2153464 AT5G05470

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004371531 – 344Eukaryotic translation initiation factor 2 subunit alphaAdd BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56Phosphoserine; by GCN21 Publication1
Modified residuei317Phosphoserine; by CK21 Publication1
Modified residuei322Phosphoserine; by CK21 Publication1

Post-translational modificationi

Phosphorylated at Ser-56 by GCN2 (Probable). Phosphorylated at Ser-317 and Ser-322 by CK2 (Probable).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FE78
PRIDEiQ9FE78

PTM databases

iPTMnetiQ9FE78

Expressioni

Gene expression databases

ExpressionAtlasiQ9FE78 baseline and differential

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G05470.1

Structurei

3D structure databases

ProteinModelPortaliQ9FE78
SMRiQ9FE78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 92S1 motifPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated

Phylogenomic databases

eggNOGiKOG2916 Eukaryota
COG1093 LUCA
HOGENOMiHOG000199476
KOiK03237
OMAiKKHGHAF
OrthoDBiEOG09360HZQ
PhylomeDBiQ9FE78

Family and domain databases

Gene3Di1.10.150.190, 1 hit
3.30.70.1130, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022967 S1_dom
IPR003029 S1_domain
IPR024055 TIF2_asu_C
IPR024054 TIF2_asu_middle_sf
IPR011488 TIF_2_asu
PANTHERiPTHR10602 PTHR10602, 1 hit
PfamiView protein in Pfam
PF07541 EIF_2_alpha, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SUPFAMiSSF110993 SSF110993, 1 hit
SSF116742 SSF116742, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FE78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPAPNLEC RMYESRYPDV DMAVMIQVKT IADMGAYVSL LEYNNIEGMI
60 70 80 90 100
LFSELSRRRI RSISSLIKVG RTEPVMVLRV DRERGYIDLS KRRVSDEDKE
110 120 130 140 150
ACEERYNKSK LVHSIMRHVA ETVGVDLEEL YVNIGWPLYK KHGHAFEAFK
160 170 180 190 200
IVVTDPDSVF DALTREVKET GPDGVEVTKV VPAVSEELKD AFLKDIRRRM
210 220 230 240 250
TPQPMKIRAD IELKCFQFDG VLHIKEAMKK AEAVGTDDCP VKIKLVAPPL
260 270 280 290 300
YVLTTHTHYK EKGIVTLNKA IEACITAIEE HKGKLVVKEG ARAVSERDDK
310 320 330 340
LLAEHMAKLR MDNEEMSGDE GSEDEEEDTG MGEVDIDGGS GIIE
Length:344
Mass (Da):38,801
Last modified:March 1, 2001 - v1
Checksum:i7D740D60FBC04514
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61R → G in AAK29673 (Ref. 1) Curated1
Sequence conflicti325Missing in AAK29673 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353096 mRNA Translation: AAK29673.1
AB005241, AB010692 Genomic DNA Translation: BAB11536.1
CP002688 Genomic DNA Translation: AED90877.1
AK117513 mRNA Translation: BAC42176.1
AF324988 mRNA Translation: AAG40340.1
BT024879 mRNA Translation: ABD85150.1
RefSeqiNP_196166.1, NM_120629.3
UniGeneiAt.48986

Genome annotation databases

EnsemblPlantsiAT5G05470.1; AT5G05470.1; AT5G05470
GeneIDi830430
GrameneiAT5G05470.1; AT5G05470.1; AT5G05470
KEGGiath:AT5G05470

Similar proteinsi

Entry informationi

Entry nameiIF2A_ARATH
AccessioniPrimary (citable) accession number: Q9FE78
Secondary accession number(s): Q9C5N6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 7, 2016
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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