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Q9FE46 (GSTFC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase F12

Short name=AtGSTF12
EC=2.5.1.18
Alternative name(s):
GST class-phi member 12
Glutathione S-transferase 26
Protein TRANSPARENT TESTA 19
Gene names
Name:GSTF12
Synonyms:GST26, TT19
Ordered Locus Names:At5g17220
ORF Names:MKP11.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the transport and/or accumulation of both anthocyanins and proanthocyanidins (PA)s in the vacuole. Functions in the cytosol to maintain the regular accumulation in the vacuole of PA precursors, such as epicatechin and glycosylated epicatechin. Ref.2 Ref.7

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subcellular location

Cytoplasmcytosol Ref.6.

Induction

By the fungal pathogen Verticillium dahliae. Ref.5

Disruption phenotype

Great reduction of anthocyanin pigments in the vegetative parts and brown pigments in the seed coat. Accumulation of unextractable proanthocyanidins. Ref.2 Ref.7

Sequence similarities

Belongs to the GST superfamily. Phi family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 214213Glutathione S-transferase F12
PRO_0000413545

Regions

Domain2 – 8281GST N-terminal
Domain89 – 214126GST C-terminal
Region11 – 122Glutathione binding By similarity
Region40 – 412Glutathione binding By similarity
Region53 – 542Glutathione binding By similarity
Region66 – 672Glutathione binding By similarity

Experimental info

Mutagenesis2051W → L in tt19-4; accumulation of unextractable proanthocyanidins. Ref.7
Sequence conflict701I → F in BAD89984. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9FE46 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: ACB25BD7CF65FB39

FASTA21424,581
        10         20         30         40         50         60 
MVVKLYGQVT AACPQRVLLC FLEKGIEFEI IHIDLDTFEQ KKPEHLLRQP FGQVPAIEDG 

        70         80         90        100        110        120 
DFKLFESRAI ARYYATKFAD QGTNLLGKSL EHRAIVDQWA DVETYYFNVL AQPLVINLII 

       130        140        150        160        170        180 
KPRLGEKCDV VLVEDLKVKL GVVLDIYNNR LSSNRFLAGE EFTMADLTHM PAMGYLMSIT 

       190        200        210 
DINQMVKARG SFNRWWEEIS DRPSWKKLMV LAGH 

« Hide

References

« Hide 'large scale' references
[1]"Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
Wagner U., Edwards R., Dixon D.P., Mauch F.
Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[2]"TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis."
Kitamura S., Shikazono N., Tanaka A.
Plant J. 37:104-114(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Ethylene perception via ETR1 is required in Arabidopsis infection by Verticillium dahliae."
Pantelides I.S., Tjamos S.E., Paplomatas E.J.
Mol. Plant Pathol. 11:191-202(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Metabolic profiling and cytological analysis of proanthocyanidins in immature seeds of Arabidopsis thaliana flavonoid accumulation mutants."
Kitamura S., Matsuda F., Tohge T., Yonekura-Sakakibara K., Yamazaki M., Saito K., Narumi I.
Plant J. 62:549-559(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"The Arabidopsis tt19-4 mutant differentially accumulates proanthocyanidin and anthocyanin through a 3' amino acid substitution in glutathione S-transferase."
Li X., Gao P., Cui D., Wu L., Parkin I., Saberianfar R., Menassa R., Pan H., Westcott N., Gruber M.Y.
Plant Cell Environ. 34:374-388(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF TRP-205.
[8]"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
Plant Physiol. Biochem. 0:0-0(2013)
Cited for: REVIEW, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF288189 mRNA. Translation: AAG30138.1.
AB117793 Genomic DNA. Translation: BAD89984.1.
AB005238 Genomic DNA. Translation: BAB10509.1.
CP002688 Genomic DNA. Translation: AED92398.1.
RefSeqNP_197224.1. NM_121728.3.
UniGeneAt.24835.

3D structure databases

ProteinModelPortalQ9FE46.
SMRQ9FE46. Positions 3-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G17220.1-P.

Proteomic databases

PRIDEQ9FE46.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G17220.1; AT5G17220.1; AT5G17220.
GeneID831586.
KEGGath:AT5G17220.

Organism-specific databases

TAIRAT5G17220.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125746.
InParanoidQ9FE46.
KOK00799.
OMAFFAMDEL.
PhylomeDBQ9FE46.

Enzyme and pathway databases

BioCycARA:AT5G17220-MONOMER.

Gene expression databases

GenevestigatorQ9FE46.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTFC_ARATH
AccessionPrimary (citable) accession number: Q9FE46
Secondary accession number(s): Q5DWV6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names