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Q9FE46

- GSTFC_ARATH

UniProt

Q9FE46 - GSTFC_ARATH

Protein

Glutathione S-transferase F12

Gene

GSTF12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Involved in the transport and/or accumulation of both anthocyanins and proanthocyanidins (PA)s in the vacuole. Functions in the cytosol to maintain the regular accumulation in the vacuole of PA precursors, such as epicatechin and glycosylated epicatechin.2 Publications

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    GO - Molecular functioni

    1. cation binding Source: TAIR
    2. glutathione transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. anthocyanin-containing compound metabolic process Source: TAIR
    2. regulation of flavonol biosynthetic process Source: TAIR
    3. response to stress Source: UniProtKB-KW
    4. response to toxic substance Source: UniProtKB-KW
    5. toxin catabolic process Source: TAIR

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Detoxification, Stress response

    Enzyme and pathway databases

    BioCyciARA:AT5G17220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase F12 (EC:2.5.1.18)
    Short name:
    AtGSTF12
    Alternative name(s):
    GST class-phi member 12
    Glutathione S-transferase 26
    Protein TRANSPARENT TESTA 19
    Gene namesi
    Name:GSTF12
    Synonyms:GST26, TT19
    Ordered Locus Names:At5g17220
    ORF Names:MKP11.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G17220.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. cytosol Source: UniProtKB-SubCell
    3. nucleus Source: TAIR
    4. plant-type vacuole membrane Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Great reduction of anthocyanin pigments in the vegetative parts and brown pigments in the seed coat. Accumulation of unextractable proanthocyanidins.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi205 – 2051W → L in tt19-4; accumulation of unextractable proanthocyanidins. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 214213Glutathione S-transferase F12PRO_0000413545Add
    BLAST

    Proteomic databases

    PRIDEiQ9FE46.

    Expressioni

    Inductioni

    By the fungal pathogen Verticillium dahliae.1 Publication

    Gene expression databases

    GenevestigatoriQ9FE46.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G17220.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FE46.
    SMRiQ9FE46. Positions 3-207.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8281GST N-terminalAdd
    BLAST
    Domaini89 – 214126GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 122Glutathione bindingBy similarity
    Regioni40 – 412Glutathione bindingBy similarity
    Regioni53 – 542Glutathione bindingBy similarity
    Regioni66 – 672Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Phi family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125746.
    InParanoidiQ9FE46.
    KOiK00799.
    OMAiFFAMDEL.
    PhylomeDBiQ9FE46.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FE46-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVKLYGQVT AACPQRVLLC FLEKGIEFEI IHIDLDTFEQ KKPEHLLRQP    50
    FGQVPAIEDG DFKLFESRAI ARYYATKFAD QGTNLLGKSL EHRAIVDQWA 100
    DVETYYFNVL AQPLVINLII KPRLGEKCDV VLVEDLKVKL GVVLDIYNNR 150
    LSSNRFLAGE EFTMADLTHM PAMGYLMSIT DINQMVKARG SFNRWWEEIS 200
    DRPSWKKLMV LAGH 214
    Length:214
    Mass (Da):24,581
    Last modified:March 1, 2001 - v1
    Checksum:iACB25BD7CF65FB39
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701I → F in BAD89984. (PubMed:14675436)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF288189 mRNA. Translation: AAG30138.1.
    AB117793 Genomic DNA. Translation: BAD89984.1.
    AB005238 Genomic DNA. Translation: BAB10509.1.
    CP002688 Genomic DNA. Translation: AED92398.1.
    RefSeqiNP_197224.1. NM_121728.3.
    UniGeneiAt.24835.

    Genome annotation databases

    EnsemblPlantsiAT5G17220.1; AT5G17220.1; AT5G17220.
    GeneIDi831586.
    KEGGiath:AT5G17220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF288189 mRNA. Translation: AAG30138.1 .
    AB117793 Genomic DNA. Translation: BAD89984.1 .
    AB005238 Genomic DNA. Translation: BAB10509.1 .
    CP002688 Genomic DNA. Translation: AED92398.1 .
    RefSeqi NP_197224.1. NM_121728.3.
    UniGenei At.24835.

    3D structure databases

    ProteinModelPortali Q9FE46.
    SMRi Q9FE46. Positions 3-207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G17220.1-P.

    Proteomic databases

    PRIDEi Q9FE46.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G17220.1 ; AT5G17220.1 ; AT5G17220 .
    GeneIDi 831586.
    KEGGi ath:AT5G17220.

    Organism-specific databases

    TAIRi AT5G17220.

    Phylogenomic databases

    eggNOGi COG0625.
    HOGENOMi HOG000125746.
    InParanoidi Q9FE46.
    KOi K00799.
    OMAi FFAMDEL.
    PhylomeDBi Q9FE46.

    Enzyme and pathway databases

    BioCyci ARA:AT5G17220-MONOMER.

    Gene expression databases

    Genevestigatori Q9FE46.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
      Wagner U., Edwards R., Dixon D.P., Mauch F.
      Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis."
      Kitamura S., Shikazono N., Tanaka A.
      Plant J. 37:104-114(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Ethylene perception via ETR1 is required in Arabidopsis infection by Verticillium dahliae."
      Pantelides I.S., Tjamos S.E., Paplomatas E.J.
      Mol. Plant Pathol. 11:191-202(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Metabolic profiling and cytological analysis of proanthocyanidins in immature seeds of Arabidopsis thaliana flavonoid accumulation mutants."
      Kitamura S., Matsuda F., Tohge T., Yonekura-Sakakibara K., Yamazaki M., Saito K., Narumi I.
      Plant J. 62:549-559(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "The Arabidopsis tt19-4 mutant differentially accumulates proanthocyanidin and anthocyanin through a 3' amino acid substitution in glutathione S-transferase."
      Li X., Gao P., Cui D., Wu L., Parkin I., Saberianfar R., Menassa R., Pan H., Westcott N., Gruber M.Y.
      Plant Cell Environ. 34:374-388(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF TRP-205.
    8. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
      Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
      Plant Physiol. Biochem. 0:0-0(2013)
      Cited for: REVIEW, NOMENCLATURE.

    Entry informationi

    Entry nameiGSTFC_ARATH
    AccessioniPrimary (citable) accession number: Q9FE46
    Secondary accession number(s): Q5DWV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3