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Protein

Glutathione S-transferase F12

Gene

GSTF12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transport and/or accumulation of both anthocyanins and proanthocyanidins (PA)s in the vacuole. Functions in the cytosol to maintain the regular accumulation in the vacuole of PA precursors, such as epicatechin and glycosylated epicatechin.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • cation binding Source: TAIR
  • glutathione transferase activity Source: GO_Central

GO - Biological processi

  • anthocyanin-containing compound metabolic process Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • regulation of flavonol biosynthetic process Source: TAIR
  • toxin catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processDetoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT5G17220-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F12 (EC:2.5.1.18)
Short name:
AtGSTF12
Alternative name(s):
GST class-phi member 12
Glutathione S-transferase 26
Protein TRANSPARENT TESTA 19
Gene namesi
Name:GSTF12
Synonyms:GST26, TT19
Ordered Locus Names:At5g17220
ORF Names:MKP11.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G17220
TAIRilocus:2167215 AT5G17220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Great reduction of anthocyanin pigments in the vegetative parts and brown pigments in the seed coat. Accumulation of unextractable proanthocyanidins.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi205W → L in tt19-4; accumulation of unextractable proanthocyanidins. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004135451 – 214Glutathione S-transferase F12Add BLAST214

Proteomic databases

PaxDbiQ9FE46

Expressioni

Inductioni

By the fungal pathogen Verticillium dahliae.1 Publication

Gene expression databases

ExpressionAtlasiQ9FE46 baseline and differential
GenevisibleiQ9FE46 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G17220.1

Structurei

3D structure databases

ProteinModelPortaliQ9FE46
SMRiQ9FE46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 82GST N-terminalAdd BLAST81
Domaini89 – 214GST C-terminalAdd BLAST126

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 12Glutathione bindingBy similarity2
Regioni40 – 41Glutathione bindingBy similarity2
Regioni53 – 54Glutathione bindingBy similarity2
Regioni66 – 67Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated

Phylogenomic databases

eggNOGiKOG0867 Eukaryota
COG0625 LUCA
HOGENOMiHOG000125746
InParanoidiQ9FE46
KOiK00799
OMAiAICRYFE
OrthoDBiEOG09360M18
PhylomeDBiQ9FE46

Family and domain databases

CDDicd03187 GST_C_Phi, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR034347 GST_Phi_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FE46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVKLYGQVT AACPQRVLLC FLEKGIEFEI IHIDLDTFEQ KKPEHLLRQP
60 70 80 90 100
FGQVPAIEDG DFKLFESRAI ARYYATKFAD QGTNLLGKSL EHRAIVDQWA
110 120 130 140 150
DVETYYFNVL AQPLVINLII KPRLGEKCDV VLVEDLKVKL GVVLDIYNNR
160 170 180 190 200
LSSNRFLAGE EFTMADLTHM PAMGYLMSIT DINQMVKARG SFNRWWEEIS
210
DRPSWKKLMV LAGH
Length:214
Mass (Da):24,581
Last modified:March 1, 2001 - v1
Checksum:iACB25BD7CF65FB39
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70I → F in BAD89984 (PubMed:14675436).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288189 mRNA Translation: AAG30138.1
AB117793 Genomic DNA Translation: BAD89984.1
AB005238 Genomic DNA Translation: BAB10509.1
CP002688 Genomic DNA Translation: AED92398.1
RefSeqiNP_197224.1, NM_121728.4
UniGeneiAt.24835

Genome annotation databases

EnsemblPlantsiAT5G17220.1; AT5G17220.1; AT5G17220
GeneIDi831586
GrameneiAT5G17220.1; AT5G17220.1; AT5G17220
KEGGiath:AT5G17220

Similar proteinsi

Entry informationi

Entry nameiGSTFC_ARATH
AccessioniPrimary (citable) accession number: Q9FE46
Secondary accession number(s): Q5DWV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health