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Q9FE46

- GSTFC_ARATH

UniProt

Q9FE46 - GSTFC_ARATH

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Protein

Glutathione S-transferase F12

Gene
GSTF12, GST26, TT19, At5g17220, MKP11.22
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the transport and/or accumulation of both anthocyanins and proanthocyanidins (PA)s in the vacuole. Functions in the cytosol to maintain the regular accumulation in the vacuole of PA precursors, such as epicatechin and glycosylated epicatechin.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. cation binding Source: TAIR
  2. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. anthocyanin-containing compound metabolic process Source: TAIR
  2. regulation of flavonol biosynthetic process Source: TAIR
  3. response to stress Source: UniProtKB-KW
  4. response to toxic substance Source: UniProtKB-KW
  5. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT5G17220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F12 (EC:2.5.1.18)
Short name:
AtGSTF12
Alternative name(s):
GST class-phi member 12
Glutathione S-transferase 26
Protein TRANSPARENT TESTA 19
Gene namesi
Name:GSTF12
Synonyms:GST26, TT19
Ordered Locus Names:At5g17220
ORF Names:MKP11.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G17220.

Subcellular locationi

Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: UniProtKB-SubCell
  3. nucleus Source: TAIR
  4. plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Great reduction of anthocyanin pigments in the vegetative parts and brown pigments in the seed coat. Accumulation of unextractable proanthocyanidins.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi205 – 2051W → L in tt19-4; accumulation of unextractable proanthocyanidins. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 214213Glutathione S-transferase F12PRO_0000413545Add
BLAST

Proteomic databases

PRIDEiQ9FE46.

Expressioni

Inductioni

By the fungal pathogen Verticillium dahliae.1 Publication

Gene expression databases

GenevestigatoriQ9FE46.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G17220.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FE46.
SMRiQ9FE46. Positions 3-207.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8281GST N-terminalAdd
BLAST
Domaini89 – 214126GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 122Glutathione binding By similarity
Regioni40 – 412Glutathione binding By similarity
Regioni53 – 542Glutathione binding By similarity
Regioni66 – 672Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125746.
InParanoidiQ9FE46.
KOiK00799.
OMAiFFAMDEL.
PhylomeDBiQ9FE46.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FE46-1 [UniParc]FASTAAdd to Basket

« Hide

MVVKLYGQVT AACPQRVLLC FLEKGIEFEI IHIDLDTFEQ KKPEHLLRQP    50
FGQVPAIEDG DFKLFESRAI ARYYATKFAD QGTNLLGKSL EHRAIVDQWA 100
DVETYYFNVL AQPLVINLII KPRLGEKCDV VLVEDLKVKL GVVLDIYNNR 150
LSSNRFLAGE EFTMADLTHM PAMGYLMSIT DINQMVKARG SFNRWWEEIS 200
DRPSWKKLMV LAGH 214
Length:214
Mass (Da):24,581
Last modified:March 1, 2001 - v1
Checksum:iACB25BD7CF65FB39
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701I → F in BAD89984. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF288189 mRNA. Translation: AAG30138.1.
AB117793 Genomic DNA. Translation: BAD89984.1.
AB005238 Genomic DNA. Translation: BAB10509.1.
CP002688 Genomic DNA. Translation: AED92398.1.
RefSeqiNP_197224.1. NM_121728.3.
UniGeneiAt.24835.

Genome annotation databases

EnsemblPlantsiAT5G17220.1; AT5G17220.1; AT5G17220.
GeneIDi831586.
KEGGiath:AT5G17220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF288189 mRNA. Translation: AAG30138.1 .
AB117793 Genomic DNA. Translation: BAD89984.1 .
AB005238 Genomic DNA. Translation: BAB10509.1 .
CP002688 Genomic DNA. Translation: AED92398.1 .
RefSeqi NP_197224.1. NM_121728.3.
UniGenei At.24835.

3D structure databases

ProteinModelPortali Q9FE46.
SMRi Q9FE46. Positions 3-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G17220.1-P.

Proteomic databases

PRIDEi Q9FE46.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G17220.1 ; AT5G17220.1 ; AT5G17220 .
GeneIDi 831586.
KEGGi ath:AT5G17220.

Organism-specific databases

TAIRi AT5G17220.

Phylogenomic databases

eggNOGi COG0625.
HOGENOMi HOG000125746.
InParanoidi Q9FE46.
KOi K00799.
OMAi FFAMDEL.
PhylomeDBi Q9FE46.

Enzyme and pathway databases

BioCyci ARA:AT5G17220-MONOMER.

Gene expression databases

Genevestigatori Q9FE46.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis."
    Kitamura S., Shikazono N., Tanaka A.
    Plant J. 37:104-114(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Ethylene perception via ETR1 is required in Arabidopsis infection by Verticillium dahliae."
    Pantelides I.S., Tjamos S.E., Paplomatas E.J.
    Mol. Plant Pathol. 11:191-202(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Metabolic profiling and cytological analysis of proanthocyanidins in immature seeds of Arabidopsis thaliana flavonoid accumulation mutants."
    Kitamura S., Matsuda F., Tohge T., Yonekura-Sakakibara K., Yamazaki M., Saito K., Narumi I.
    Plant J. 62:549-559(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "The Arabidopsis tt19-4 mutant differentially accumulates proanthocyanidin and anthocyanin through a 3' amino acid substitution in glutathione S-transferase."
    Li X., Gao P., Cui D., Wu L., Parkin I., Saberianfar R., Menassa R., Pan H., Westcott N., Gruber M.Y.
    Plant Cell Environ. 34:374-388(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF TRP-205.
  8. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
    Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
    Plant Physiol. Biochem. 0:0-0(2013)
    Cited for: REVIEW, NOMENCLATURE.

Entry informationi

Entry nameiGSTFC_ARATH
AccessioniPrimary (citable) accession number: Q9FE46
Secondary accession number(s): Q5DWV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi