Reviewed, UniProtKB/Swiss-Prot Q9FDX1 (SKIP1_ARATH)

Names and origin

Protein names

Recommended name:
    F-box protein SKIP1
Alternative name(s):
    SKP1-interacting partner 1

Gene names

Name:	SKIP1
Ordered Locus Names:	At5g57900

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1 and SKP1B/ASK2.
Subcellular location	Nucleus By similarity.
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	SCF ubiquitin ligase complex Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.1 Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1A	Q39255	2	EBI-604228,EBI-532357
SKP1B	Q9FHW7	2	EBI-604228,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	F-box protein SKIP1		PRO_0000272203
Regions
	Domain	11 – 52	42	F-box; degenerate

Sequences

	Sequence		Length	Mass (Da)
	Q9FDX1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F629B3595DCF5EC5	FASTA	300	34,565
	10 20 30 40 50 60 MEEDGSDWGG LAPEILINII SRLTIQELWT GPMFVQKSWL TVCRDPYLWS IFDLEPWFDS 70 80 90 100 110 120 YPESTHLWSP EFEQKVDLML RSVVDWSEGG LTKIRVRHCS DHALSYAADR CPNLQVLAIR 130 140 150 160 170 180 SSPNVTDASM TKIAFRCRSL KELDISYCHE ISHDTLVMIG RNCPNLRILK RNLMDWSSRH 190 200 210 220 230 240 IGSVPTEYLD ACPQDGDTEA DAIGKHMINL EHLEIQFSRL SVKGLASICE GCPKLEYLDL 250 260 270 280 290 300 FGCVHLSSRD ITSNVSRLKW LKEVKKPDVY VPRSGDVAQT ERYGHWRLYD ERFDIQAMRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase." Farras R., Ferrando A., Jasik J., Kleinow T., Oekresz L., Tiburcio A., Salchert K., del Pozo C., Schell J., Koncz C. EMBO J. 20:2742-2756(2001) [PubMed: 11387208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SKP1A/ASK1.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF263377 mRNA. Translation: AAG21976.1. AB013396 Genomic DNA. Translation: BAB08860.1. AK229464 mRNA. Translation: BAF01322.1. BT026067 mRNA. Translation: ABG48423.1.
IPI	IPI00521503.
RefSeq	NP_568870.1.
UniGene	At.7657
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FDX1. 3 interactions.
Proteomic databases
PRIDE	Q9FDX1.
Genome annotation databases
GeneID	835901.
GenomeReviews	Gene locus AT5G57900 in contig BA000015_GR.
KEGG	ath:AT5G57900.
NMPDR	fig|3702.1.peg.27753.
Organism-specific databases
TAIR	At5g57900.
Phylogenomic databases
OMA	Q9FDX1. CANLTSR.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKIP1_ARATH

Accession

Primary (citable) accession number: Q9FDX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)