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Q9FDL5 (PUR2_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:ZMO0299
OrganismZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]
Taxonomic identifier264203 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAG02154.1 differs from that shown. Reason: Frameshift at positions 86, 99 and 123.

The sequence AAV88923.2 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151507

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding133 – 19361ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Experimental info

Sequence conflict2351R → S in AAG02154. Ref.1
Sequence conflict3951D → Y in AAG02154. Ref.1
Sequence conflict4051D → V in AAG02154. Ref.1
Sequence conflict4131G → E in AAG02154. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9FDL5 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: C31176955B0D9FC2

FASTA41744,044
        10         20         30         40         50         60 
MNVLLIGTGG REHALAWKIA QSPSLDTFYA TTGNPGIAKL AKPADITVTD HAAVVRFCQD 

        70         80         90        100        110        120 
NAIDLVVIGP EAPLVDGLGN SLRAASIAVF GPDKAAAQLE GSKGFTKDLC ARYNIPTARY 

       130        140        150        160        170        180 
QRCRSAEEAK AALDNFSTPV VIKADGLAGG KGVIIAESRL EAEQAIQDMT AGAFGKAGLE 

       190        200        210        220        230        240 
IVVEEFMQGE EASFFAISDG ETVRPFGTAR DHKRVGDGDT GPNTGGMGAY SPATRLTADL 

       250        260        270        280        290        300 
EKRVMQEIVT PTVQAMKEQG MPFVGILYAG LMLTSEGPKL IEYNARLGDP ECQVLMMRLE 

       310        320        330        340        350        360 
SDILPLLYAA ATGKLQAQPE PTFSKDYAVT VVIAAQGYPA SPKKGGIITR LNEAEANGAV 

       370        380        390        400        410 
VFQAGTTLDN QTLKANGGRV LNVTAKAADF NAARDLAYQA VNVIDYPDGF WRGDIGL 

« Hide

References

« Hide 'large scale' references
[1]Lee H.J., Kang H.S.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[2]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF212041 Genomic DNA. Translation: AAG02154.1. Frameshift.
AE008692 Genomic DNA. Translation: AAV88923.2. Different initiation.
RefSeqYP_162034.2. NC_006526.2.

3D structure databases

ProteinModelPortalQ9FDL5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264203.ZMO0299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV88923; AAV88923; ZMO0299.
GeneID3188074.
KEGGzmo:ZMO0299.
PATRIC32565942. VBIZymMob102260_0286.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAYKGFLYA.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_ZYMMO
AccessionPrimary (citable) accession number: Q9FDL5
Secondary accession number(s): Q5NQT1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways