Q9FDL5 (PUR2_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	ZMO0299

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]

Taxonomic identifier

264203 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence AAG02154.1 differs from that shown. Reason: Frameshift at positions 86, 99 and 123. The sequence AAV88923.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151507
Regions
	Domain	107 – 312	206	ATP-grasp
	Nucleotide binding	133 – 193	61	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity
Experimental info
	Sequence conflict	235	1	R → S in AAG02154. Ref.1
	Sequence conflict	395	1	D → Y in AAG02154. Ref.1
	Sequence conflict	405	1	D → V in AAG02154. Ref.1
	Sequence conflict	413	1	G → E in AAG02154. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FDL5 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: C31176955B0D9FC2	FASTA	417	44,044
	10 20 30 40 50 60 MNVLLIGTGG REHALAWKIA QSPSLDTFYA TTGNPGIAKL AKPADITVTD HAAVVRFCQD 70 80 90 100 110 120 NAIDLVVIGP EAPLVDGLGN SLRAASIAVF GPDKAAAQLE GSKGFTKDLC ARYNIPTARY 130 140 150 160 170 180 QRCRSAEEAK AALDNFSTPV VIKADGLAGG KGVIIAESRL EAEQAIQDMT AGAFGKAGLE 190 200 210 220 230 240 IVVEEFMQGE EASFFAISDG ETVRPFGTAR DHKRVGDGDT GPNTGGMGAY SPATRLTADL 250 260 270 280 290 300 EKRVMQEIVT PTVQAMKEQG MPFVGILYAG LMLTSEGPKL IEYNARLGDP ECQVLMMRLE 310 320 330 340 350 360 SDILPLLYAA ATGKLQAQPE PTFSKDYAVT VVIAAQGYPA SPKKGGIITR LNEAEANGAV 370 380 390 400 410 VFQAGTTLDN QTLKANGGRV LNVTAKAADF NAARDLAYQA VNVIDYPDGF WRGDIGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Lee H.J., Kang H.S. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[2]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF212041 Genomic DNA. Translation: AAG02154.1. Frameshift. AE008692 Genomic DNA. Translation: AAV88923.2. Different initiation.
RefSeq	YP_162034.2. NC_006526.2.
3D structure databases
ProteinModelPortal	Q9FDL5.
ModBase	Search...
Protein-protein interaction databases
STRING	264203.ZMO0299.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV88923; AAV88923; ZMO0299.
GeneID	3188074.
KEGG	zmo:ZMO0299.
PATRIC	32565942. VBIZymMob102260_0286.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033463.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	CLSK2524527.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_ZYMMO

Accession

Primary (citable) accession number: Q9FDL5
Secondary accession number(s): Q5NQT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program