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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei341D-inositol 3-phosphateUniRule annotation1
Binding sitei48UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei1031D-inositol 3-phosphateUniRule annotation1
Binding sitei1361D-inositol 3-phosphateUniRule annotation1
Binding sitei1601D-inositol 3-phosphateUniRule annotation1
Binding sitei1801D-inositol 3-phosphateUniRule annotation1
Binding sitei267UDP-GlcNAcUniRule annotation1
Binding sitei272UDP-GlcNAcUniRule annotation1
Binding sitei333UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi342Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi343Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi345Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei355UDP-GlcNAcUniRule annotation1
Binding sitei363UDP-GlcNAcUniRule annotation1
Metal bindingi369MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:SCO4204
ORF Names:2SCD46.18
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001621 – 457D-inositol 3-phosphate glycosyltransferaseAdd BLAST457

Expressioni

Inductioni

Transiently induced by thiol-oxidant diamide, under control of SigR. Also induced when mycothiol is oxidized or conjugated.1 Publication

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO4204.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 41UDP-GlcNAc bindingUniRule annotation2
Regioni45 – 501D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
InParanoidiQ9FCG5.
KOiK15521.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FCG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQYVSRLGR RSPAASPRLR LNRKPRRVAM LSVHTSPLHQ PGTGDAGGMN
60 70 80 90 100
VYIVELAQRL AAINIEVEIF TRATTAALPP AVELAPGVLV RHVDAGPYEG
110 120 130 140 150
LAKEELPAQL CAFTHGVMQA WAGHRPGHYD LVHSHYWLSG HVGWLAAQRW
160 170 180 190 200
GAPLVHAMHT MAKVKNANLA DGDTPEPAAR VIGETQIVAA SDRLIANTAE
210 220 230 240 250
EADELVRHYA ADPDKVAVVH PGVNLERFRP FPKGRVPGPG QHGNARAAAR
260 270 280 290 300
ARLGLPQDAL IPLFAGRIQP LKAPDILLRA VAVLLDERPE LRSRIVVPVV
310 320 330 340 350
GGPSGSGLAK PEGLQKLAAR LGIADVVRFR PPVGQEQLAD WFRAASVLVM
360 370 380 390 400
PSYSESFGLV AIEAQAAGTP VLAAAVGGLP VAVRDGHTGR LVHGHDPAAY
410 420 430 440 450
ARVLRDFADN PDLTPRMGDA AARHAQSFGW DSAAATTADV YTAAIQSYRR

RVRSHHG
Length:457
Mass (Da):48,864
Last modified:July 24, 2013 - v2
Checksum:i8763E5E709B4F7B1
GO

Sequence cautioni

The sequence CAC04040 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939119 Genomic DNA. Translation: CAC04040.1. Different initiation.
RefSeqiNP_628379.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAC04040; CAC04040; CAC04040.
GeneIDi1099644.
KEGGisco:SCO4204.
PATRICi23738206. VBIStrCoe124346_4268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939119 Genomic DNA. Translation: CAC04040.1. Different initiation.
RefSeqiNP_628379.1. NC_003888.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO4204.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC04040; CAC04040; CAC04040.
GeneIDi1099644.
KEGGisco:SCO4204.
PATRICi23738206. VBIStrCoe124346_4268.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
InParanoidiQ9FCG5.
KOiK15521.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_STRCO
AccessioniPrimary (citable) accession number: Q9FCG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 24, 2013
Last modified: September 7, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.