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Q9FCC3 (BIOB_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:SCO1244
ORF Names:2SCG1.19
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Biotin synthase HAMAP-Rule MF_01694
PRO_0000381664

Sites

Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1091Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2021Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2721Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FCC3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 26369EAEAB7B91FD

FASTA40742,903
        10         20         30         40         50         60 
MDLLNTLVDK GLRRETPTRE EALAVLVTSD DDLLDVVAAA GKVRRHWFGR RVKLNYLVNL 

        70         80         90        100        110        120 
KSGLCPEDCS YCSQRLGSKA EILKYTWLKP DQASAAAAAG VSGGAKRVCL VASGRGPTDR 

       130        140        150        160        170        180 
DVDRVSDTIK AIKEQNESVE VCACLGLLSD GQAERLREAG ADAYNHNLNT SESTYGDITT 

       190        200        210        220        230        240 
THTYADRVDT VNKAHAAGLS ACSGLIAGMG ESDEDLVDVV FSLRELDPDS VPVNFLIPME 

       250        260        270        280        290        300 
GTPLAKEWHL TPQRCLRILA MTRFVCPDVE VRIAGGREVH LRTMQPLALH LANSIFLGDY 

       310        320        330        340        350        360 
LTSEGQAGHA DLELIADAGF EVEGAGEVTL PEHRASVRGG GCGSHEGGGA CGSHDGADGD 

       370        380        390        400 
AGCGSHAGGG VCAPAPAATT PRPAEEPRTD LVAVRRRGAG TDLAPNA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL939108 Genomic DNA. Translation: CAC01468.1.
RefSeqNP_625532.1. NC_003888.3.

3D structure databases

ProteinModelPortalQ9FCC3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO1244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC01468; CAC01468; CAC01468.
GeneID1096667.
KEGGsco:SCO1244.
PATRIC23732042. VBIStrCoe124346_1244.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMACKEDCIF.
OrthoDBEOG622PMP.
PhylomeDBQ9FCC3.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_STRCO
AccessionPrimary (citable) accession number: Q9FCC3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways