Reviewed, UniProtKB/Swiss-Prot Q9FCB1 (DNLI_STRCO)

Names and origin

Protein names

Recommended name:
    Probable DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	SCO1202
ORF Names:	2SCG58.02

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Probable DNA ligase HAMAP MF_00407		PRO_0000059624
Sites
	Active site	210	1	N6-AMP-lysine intermediate By similarity
	Binding site	208	1	ATP By similarity
	Binding site	215	1	ATP By similarity
	Binding site	230	1	ATP By similarity
	Binding site	259	1	ATP By similarity
	Binding site	299	1	ATP By similarity
	Binding site	374	1	ATP By similarity
	Binding site	380	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FCB1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7A85CA68DC760FF9	FASTA	512	54,696
	10 20 30 40 50 60 MLLARLAQVS REVAATSARS RKTVLLAELF REAEAADVPV VIPYLAGRLP QGRIGVGWKV 70 80 90 100 110 120 LSRRVPPADA PTLTVRDVDA RLTRLGAVSG AGSQAERTRL VGELMGAATE DEQRFLIGLL 130 140 150 160 170 180 TGEVRQGALD AAAVEGLAAA TDAPPADVRR AVMLAGSLQT VAEALLADGP GALDRFRLTV 190 200 210 220 230 240 GQPVLPMLAH SASSVAEAVG KLGAAAVEEK LDGIRVQVHR DGGTVRIYTR TLDDITDRLP 250 260 270 280 290 300 EVTEAALALP GERFILDGEA ISLDADGRPR SFQETAGRVG SRTDVATAAR AVPVSAVFFD 310 320 330 340 350 360 VLSVDGRDLL DLPLTERHAE LARLVPEPLR VRRTLVHGPE DTGAAEEFLA RTLARGHEGV 370 380 390 400 410 420 VVKGLDAAYS AGRRGASWLK VKPVHTLDLV VLAAEWGHGR RTGKLSNLHL GARTADGSFA 430 440 450 460 470 480 MLGKTFKGMT DALLTWQTER LKELAVEEHG WGVTVRPELV VEIAYDGLQR STRYPAGVTL 490 500 510 RFARVVRYRE DKRPEDADTV DTLLAAHPGV AP

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939108 Genomic DNA. Translation: CAC01484.1.
RefSeq	NP_625491.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1096625.
GenomeReviews	Gene locus SCO1202 in contig AL645882_GR.
KEGG	sco:SCO1202.
NMPDR	fig|100226.1.peg.1169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9FCB1.
OMA	Q9FCB1. SNLHLGA.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO1202-MON.
BRENDA	6.5.1.1. 1084.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_STRCO

Accession

Primary (citable) accession number: Q9FCB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)