Reviewed, UniProtKB/Swiss-Prot Q9FCA2 (DEF2_STRCO)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 2
      Short name=PDF 2
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	SCO1211
ORF Names:	2SCG58.11c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082853
Sites
	Active site	147	1	By similarity
	Metal binding	104	1	Iron By similarity
	Metal binding	146	1	Iron By similarity
	Metal binding	150	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FCA2-1 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 8ABB3827812788F2	FASTA	179	19,476
	10 20 30 40 50 60 MRQGSIPGAH GRVRPLGLLG DPVLHARCAE VTDFGPELAA LVEDLFATMY AAHGVGLAAN 70 80 90 100 110 120 QVGEAVRVFV YDCPDDEDER HLGHVVNPRL VETGGVVVRG PEGCLSLPGL EAGTERYDEA 130 140 150 160 170 VVTGFTVAGE PVTVRGTGFF ARCLQHECDH LEGRVYADRL TGRRHRKLMR QVARASWHR

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939108 Genomic DNA. Translation: CAC01493.1. Different initiation.
RefSeq	NP_625500.1.
3D structure databases
HSSP	HSSP built from PDB template 1LME based on UniProtKB P96113.
ModBase	Search...
Genome annotation databases
GeneID	1096634.
GenomeReviews	Gene locus SCO1211 in contig AL645882_GR.
KEGG	sco:SCO1211.
NMPDR	fig|100226.1.peg.1178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9FCA2.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO1211-MON.
BRENDA	3.5.1.88. 1084.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_STRCO

Accession

Primary (citable) accession number: Q9FCA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)