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Protein

ATP-dependent 6-phosphofructokinase 3

Gene

pfkA3

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase 2 (pgi2), Glucose-6-phosphate isomerase 1 (pgi1)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfkA1), ATP-dependent 6-phosphofructokinase 2 (pfkA2), ATP-dependent 6-phosphofructokinase 3 (pfkA3)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Sitei104Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei266Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.11 5998
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 3UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 3UniRule annotation
Short name:
Phosphofructokinase 3UniRule annotation
Alternative name(s):
Phosphohexokinase 3UniRule annotation
Gene namesi
Name:pfkA3UniRule annotation
Synonyms:pfk3
Ordered Locus Names:SCO1214
ORF Names:2SCG58.14
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No effect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119881 – 341ATP-dependent 6-phosphofructokinase 3Add BLAST341

Proteomic databases

PRIDEiQ9FC99

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO1214

Structurei

3D structure databases

ProteinModelPortaliQ9FC99
SMRiQ9FC99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni272 – 275Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248869
InParanoidiQ9FC99
KOiK21071
OMAiALYDLTF
OrthoDBiPOG091H01AC
PhylomeDBiQ9FC99

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02483 PFK_mixed, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FC99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVLTSGG DCPGLNAVIR SVVHRAVVDH GDEVIGFRDG WKGLLECDYL
60 70 80 90 100
KLDLDAVGGI LARGGTILGS SRVRPEHLRD GVERARGHVE ELGLDAIIPI
110 120 130 140 150
GGEGTLKAAR LLSDNGLPIV GVPKTIDNDI AVTDVTFGFD TAVTVATEAL
160 170 180 190 200
DRLKTTAESH QRVLIVEVMG RHTGWIALHS GMAAGAHAVV VPERPFDIDE
210 220 230 240 250
LTAKVGERFS AGKRFAIVVA AEGAKPKAGT MDFDEGGKDV YGHERFAGIA
260 270 280 290 300
RQLSIELEER LGKEARPVIL GHVQRGGTPT AYDRVLATRF GWHAVEAVHR
310 320 330 340
GEFGKMTALR GTDIEMVSLA DAVESLKTVP DARYAEAECV L
Length:341
Mass (Da):36,432
Last modified:March 1, 2001 - v1
Checksum:iE3050D37BDB6F9F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939108 Genomic DNA Translation: CAC01496.1
RefSeqiNP_625503.1, NC_003888.3
WP_003977617.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAC01496; CAC01496; CAC01496
GeneIDi1096637
KEGGisco:SCO1214
PATRICifig|100226.15.peg.1213

Similar proteinsi

Entry informationi

Entry nameiPFKA3_STRCO
AccessioniPrimary (citable) accession number: Q9FC99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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