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Protein

ATP-dependent 6-phosphofructokinase 3

Gene

pfkA3

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
Sitei104 – 1041Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Binding sitei266 – 2661Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPUniRule annotation
Nucleotide bindingi102 – 1054ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.11. 5998.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 3UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 3UniRule annotation
Short name:
Phosphofructokinase 3UniRule annotation
Alternative name(s):
Phosphohexokinase 3UniRule annotation
Gene namesi
Name:pfkA3UniRule annotation
Synonyms:pfk3
Ordered Locus Names:SCO1214
ORF Names:2SCG58.14
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No effect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341ATP-dependent 6-phosphofructokinase 3PRO_0000111988Add
BLAST

Proteomic databases

PRIDEiQ9FC99.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO1214.

Structurei

3D structure databases

ProteinModelPortaliQ9FC99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1273Substrate bindingUniRule annotation
Regioni169 – 1713Substrate bindingUniRule annotation
Regioni272 – 2754Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
InParanoidiQ9FC99.
KOiK00850.
OMAiHERFAGI.
OrthoDBiEOG644ZRM.
PhylomeDBiQ9FC99.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FC99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGVLTSGG DCPGLNAVIR SVVHRAVVDH GDEVIGFRDG WKGLLECDYL
60 70 80 90 100
KLDLDAVGGI LARGGTILGS SRVRPEHLRD GVERARGHVE ELGLDAIIPI
110 120 130 140 150
GGEGTLKAAR LLSDNGLPIV GVPKTIDNDI AVTDVTFGFD TAVTVATEAL
160 170 180 190 200
DRLKTTAESH QRVLIVEVMG RHTGWIALHS GMAAGAHAVV VPERPFDIDE
210 220 230 240 250
LTAKVGERFS AGKRFAIVVA AEGAKPKAGT MDFDEGGKDV YGHERFAGIA
260 270 280 290 300
RQLSIELEER LGKEARPVIL GHVQRGGTPT AYDRVLATRF GWHAVEAVHR
310 320 330 340
GEFGKMTALR GTDIEMVSLA DAVESLKTVP DARYAEAECV L
Length:341
Mass (Da):36,432
Last modified:February 28, 2001 - v1
Checksum:iE3050D37BDB6F9F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939108 Genomic DNA. Translation: CAC01496.1.
RefSeqiNP_625503.1. NC_003888.3.

Genome annotation databases

GeneIDi1096637.
KEGGisco:SCO1214.
PATRICi23731980. VBIStrCoe124346_1213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939108 Genomic DNA. Translation: CAC01496.1.
RefSeqiNP_625503.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ9FC99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO1214.

Proteomic databases

PRIDEiQ9FC99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1096637.
KEGGisco:SCO1214.
PATRICi23731980. VBIStrCoe124346_1213.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
InParanoidiQ9FC99.
KOiK00850.
OMAiHERFAGI.
OrthoDBiEOG644ZRM.
PhylomeDBiQ9FC99.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BRENDAi2.7.1.11. 5998.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion."
    Borodina I., Siebring J., Zhang J., Smith C.P., van Keulen G., Dijkhuizen L., Nielsen J.
    J. Biol. Chem. 283:25186-25199(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPFKA3_STRCO
AccessioniPrimary (citable) accession number: Q9FC99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2001
Last sequence update: February 28, 2001
Last modified: March 31, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.