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Q9FC99 (PFKA3_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 3

Short name=ATP-PFK 3
Short name=Phosphofructokinase 3
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 3
Gene names
Name:pfkA3
Synonyms:pfk3
Ordered Locus Names:SCO1214
ORF Names:2SCG58.14
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Disruption phenotype

No effect. Ref.2

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341ATP-dependent 6-phosphofructokinase 3 HAMAP-Rule MF_01976
PRO_0000111988

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region272 – 2754Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site101ATP; via amide nitrogen By similarity
Binding site1621Substrate; shared with dimeric partner By similarity
Binding site2221Substrate By similarity
Binding site2661Substrate; shared with dimeric partner By similarity
Site1041Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9FC99 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: E3050D37BDB6F9F0

FASTA34136,432
        10         20         30         40         50         60 
MRIGVLTSGG DCPGLNAVIR SVVHRAVVDH GDEVIGFRDG WKGLLECDYL KLDLDAVGGI 

        70         80         90        100        110        120 
LARGGTILGS SRVRPEHLRD GVERARGHVE ELGLDAIIPI GGEGTLKAAR LLSDNGLPIV 

       130        140        150        160        170        180 
GVPKTIDNDI AVTDVTFGFD TAVTVATEAL DRLKTTAESH QRVLIVEVMG RHTGWIALHS 

       190        200        210        220        230        240 
GMAAGAHAVV VPERPFDIDE LTAKVGERFS AGKRFAIVVA AEGAKPKAGT MDFDEGGKDV 

       250        260        270        280        290        300 
YGHERFAGIA RQLSIELEER LGKEARPVIL GHVQRGGTPT AYDRVLATRF GWHAVEAVHR 

       310        320        330        340 
GEFGKMTALR GTDIEMVSLA DAVESLKTVP DARYAEAECV L 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL939108 Genomic DNA. Translation: CAC01496.1.
RefSeqNP_625503.1. NC_003888.3.

3D structure databases

ProteinModelPortalQ9FC99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO1214.

Proteomic databases

PRIDEQ9FC99.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC01496; CAC01496; CAC01496.
GeneID1096637.
KEGGsco:SCO1214.
PATRIC23731980. VBIStrCoe124346_1213.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMARMARRFQ.
OrthoDBEOG644ZRM.
PhylomeDBQ9FC99.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02483. PFK_mixed. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA3_STRCO
AccessionPrimary (citable) accession number: Q9FC99
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways