Q9FC99 (K6PF3_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 3
EC=2.7.1.11

Alternative name(s):
Phosphofructokinase 3
Phosphohexokinase 3

Gene names

Name:	pfkA3
Synonyms:	pfk3
Ordered Locus Names:	SCO1214
ORF Names:	2SCG58.14

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	6-phosphofructokinase 3 HAMAP MF_00339		PRO_0000111988
Regions
	Nucleotide binding	20 – 24	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	266	1	Substrate By similarity
	Binding site	272	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FC99 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E3050D37BDB6F9F0	FASTA	341	36,432
	10 20 30 40 50 60 MRIGVLTSGG DCPGLNAVIR SVVHRAVVDH GDEVIGFRDG WKGLLECDYL KLDLDAVGGI 70 80 90 100 110 120 LARGGTILGS SRVRPEHLRD GVERARGHVE ELGLDAIIPI GGEGTLKAAR LLSDNGLPIV 130 140 150 160 170 180 GVPKTIDNDI AVTDVTFGFD TAVTVATEAL DRLKTTAESH QRVLIVEVMG RHTGWIALHS 190 200 210 220 230 240 GMAAGAHAVV VPERPFDIDE LTAKVGERFS AGKRFAIVVA AEGAKPKAGT MDFDEGGKDV 250 260 270 280 290 300 YGHERFAGIA RQLSIELEER LGKEARPVIL GHVQRGGTPT AYDRVLATRF GWHAVEAVHR 310 320 330 340 GEFGKMTALR GTDIEMVSLA DAVESLKTVP DARYAEAECV L

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939108 Genomic DNA. Translation: CAC01496.1.
RefSeq	NP_625503.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q9FC99.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1096637.
GenomeReviews	Gene locus SCO1214 in contig AL645882_GR.
KEGG	sco:SCO1214.
NMPDR	fig|100226.1.peg.1181.
PATRIC	23731980. VBIStrCoe124346_1213.
Phylogenomic databases
HOGENOM	HBG405044.
OMA	HERFAGI.
PhylomeDB	Q9FC99.
ProtClustDB	PRK03202.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
KO	K00850.
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF3_STRCO

Accession

Primary (citable) accession number: Q9FC99

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program