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Q9FC99

- PFKA3_STRCO

UniProt

Q9FC99 - PFKA3_STRCO

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Protein
ATP-dependent 6-phosphofructokinase 3
Gene
pfkA3, pfk3, SCO1214, 2SCG58.14
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Sitei104 – 1041Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei127 – 1271Proton acceptor By similarity
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarity
Binding sitei222 – 2221Substrate By similarity
Binding sitei266 – 2661Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 3 (EC:2.7.1.11)
Short name:
ATP-PFK 3
Short name:
Phosphofructokinase 3
Alternative name(s):
Phosphohexokinase 3
Gene namesi
Name:pfkA3
Synonyms:pfk3
Ordered Locus Names:SCO1214
ORF Names:2SCG58.14
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No effect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341ATP-dependent 6-phosphofructokinase 3UniRule annotation
PRO_0000111988Add
BLAST

Proteomic databases

PRIDEiQ9FC99.

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO1214.

Structurei

3D structure databases

ProteinModelPortaliQ9FC99.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1273Substrate binding By similarity
Regioni169 – 1713Substrate binding By similarity
Regioni272 – 2754Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRMARRFQ.
OrthoDBiEOG644ZRM.
PhylomeDBiQ9FC99.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FC99-1 [UniParc]FASTAAdd to Basket

« Hide

MRIGVLTSGG DCPGLNAVIR SVVHRAVVDH GDEVIGFRDG WKGLLECDYL    50
KLDLDAVGGI LARGGTILGS SRVRPEHLRD GVERARGHVE ELGLDAIIPI 100
GGEGTLKAAR LLSDNGLPIV GVPKTIDNDI AVTDVTFGFD TAVTVATEAL 150
DRLKTTAESH QRVLIVEVMG RHTGWIALHS GMAAGAHAVV VPERPFDIDE 200
LTAKVGERFS AGKRFAIVVA AEGAKPKAGT MDFDEGGKDV YGHERFAGIA 250
RQLSIELEER LGKEARPVIL GHVQRGGTPT AYDRVLATRF GWHAVEAVHR 300
GEFGKMTALR GTDIEMVSLA DAVESLKTVP DARYAEAECV L 341
Length:341
Mass (Da):36,432
Last modified:March 1, 2001 - v1
Checksum:iE3050D37BDB6F9F0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939108 Genomic DNA. Translation: CAC01496.1.
RefSeqiNP_625503.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAC01496; CAC01496; CAC01496.
GeneIDi1096637.
KEGGisco:SCO1214.
PATRICi23731980. VBIStrCoe124346_1213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939108 Genomic DNA. Translation: CAC01496.1 .
RefSeqi NP_625503.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q9FC99.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO1214.

Proteomic databases

PRIDEi Q9FC99.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC01496 ; CAC01496 ; CAC01496 .
GeneIDi 1096637.
KEGGi sco:SCO1214.
PATRICi 23731980. VBIStrCoe124346_1213.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi RMARRFQ.
OrthoDBi EOG644ZRM.
PhylomeDBi Q9FC99.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Antibiotic overproduction in Streptomyces coelicolor A3(2) mediated by phosphofructokinase deletion."
    Borodina I., Siebring J., Zhang J., Smith C.P., van Keulen G., Dijkhuizen L., Nielsen J.
    J. Biol. Chem. 283:25186-25199(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPFKA3_STRCO
AccessioniPrimary (citable) accession number: Q9FC99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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