Reviewed, UniProtKB/Swiss-Prot Q9FBT8 (ILVC2_STRCO)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase 2
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 2
    Alpha-keto-beta-hydroxylacil reductoisomerase 2

Gene names

Name:	ilvC2
Ordered Locus Names:	SCO7154
ORF Names:	SC9A4.16c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Ketol-acid reductoisomerase 2 HAMAP MF_00435		PRO_0000151364
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FBT8-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D984A88B0BFF9655	FASTA	332	36,348
	10 20 30 40 50 60 MAELFYDADA DLSIIQGRKV AVIGYGSQGH AHALSLRDSG VDVRVGLHEG SKSKAKAEEQ 70 80 90 100 110 120 GLRVVPVAEA AAEADVIMIL VPDPIQAEVY EKDIKDNLKD GDALFFGHGL NIRYGFVKPP 130 140 150 160 170 180 AGVDVCMVAP KGPGHLVRRQ YEEGRGVPCL VAVEQDATGN AFALALSYAK GIGGTRAGVI 190 200 210 220 230 240 RTTFTEETET DLFGEQAVLA GGVTALVKAG FETLTEAGYQ PEIAYFECLH ELKLIVDLMY 250 260 270 280 290 300 EGGLEKMRWS ISETAEWGDY VTGPRIITDV TKAEMRKVLA EIQDGTFAKN WMDEYHGGLK 310 320 330 KYNEYKKQDS EHLLETTGKE LRKLMSWVDE EA

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939130 Genomic DNA. Translation: CAC01643.1.
RefSeq	NP_631213.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1102592.
GenomeReviews	Gene locus SCO7154 in contig AL645882_GR.
KEGG	sco:SCO7154.
NMPDR	fig|100226.1.peg.7088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9FBT8.
OMA	Q9FBT8. TEIQQGE.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO7154-MON.
BRENDA	1.1.1.86. 1084.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC2_STRCO

Accession

Primary (citable) accession number: Q9FBT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)